ABSTRACT
PURPOSE: Gorham-Stout disease is a very rare disorder characterized by progressive bone erosion and angiomatous proliferation; its etiopathogenesis is still unknown, and diagnosis is still performed by exclusion criteria. The alteration of bone remodeling activity has been reported in patients; in this study, we characterized circulating osteoclast and osteogenic precursors that could be important to better understand the osteolysis observed in patients. METHODS: Flow cytometry analysis of PBMC (Peripheral Blood Mononuclear Cells) was performed to characterize circulating osteoclast and osteogenic precursors in GSD patients (n = 9) compared to healthy donors (n = 55). Moreover, ELISA assays were assessed to evaluate serum levels of bone markers including RANK-L (Receptor activator of NF-κB ligand), OPG (Osteoprotegerin), BALP (Bone Alkaline Phosphatase) and OCN (Osteocalcin). RESULTS: We found an increase of CD16-/CD14+CD11b+ and CD115+/CD14+CD11b+ osteoclast precursors in GSD patients, with high levels of serum RANK-L that could reflect the increase of bone resorption activity observed in patients. Moreover, no significant alterations were found regarding osteogenic precursors and serum levels of BALP and OCN. CONCLUSION: The analysis of circulating bone cell precursors, as well as of RANK-L, could be relevant as an additional diagnostic tool for these patients and could be exploited for therapeutic purposes.
Subject(s)
Antibodies, Viral/blood , COVID-19/blood , Chilblains/blood , Immunoglobulin A/blood , SARS-CoV-2/immunology , Adolescent , Asymptomatic Infections , COVID-19/complications , COVID-19/diagnosis , Chilblains/virology , Child , Female , Humans , Immunoglobulin G/blood , Male , Nail Diseases/blood , Nail Diseases/virology , Nasopharynx/virology , ToesABSTRACT
Foram avaliados 11 atletas de elite praticantes de maratona através do protocolo do Grupo de Medicina Esportiva do IOT-HC/FMUSP, criado para a avaliaçäo de 500 atletas jovens (8 a 18 anos), de dez modalidades esportivas (5 coletivas e 5 individuais). Os atletas responderam a questionário geral, ortopédico, nutricional e psicológico. Passaram por exame clínico e ortopédico completo. Foram submetidos a mensuraçäo antropométrica e de flexibilidade. Foram feitas a avaliaçäo e orientaçäo nutricional ao lado de provas laboratoriais, densitometria, cintilografia e eletrocardiografia. Para a análise do dicionamento cardiorrespiratório foram utilizados os testes de Harvard e Ruffier modificados, obtendo-se nas provas de pista a avaliaçäo final. Os 11 atletas mostraram-se, em sua maioria, aptos para a prática da maratona, com pequenas correçöes já transmitidas pelos avaliadores.