ABSTRACT
This work presents data on the relationship between the oligomeric state and catalytic activity of ß-like DNA polymerases isolated from three cancer cell lines upon replacement of magnesium ions with iron. It was shown that the suppression of the catalytic activity of ß-like DNA polymerases in the presence of divalent iron ions is related to enzyme oligomerization that can be responsible for the suppression of the magnetic isotope effect.
Subject(s)
DNA-Directed DNA Polymerase , Magnesium , Ions , Iron , Isotopes , Magnesium/metabolism , Magnesium/pharmacologyABSTRACT
The fundamental possibility of obtaining ultrashort (50n-100n) single-stranded polydeoxyribonucleotides by the method of radiation-chemical destruction of total yeast DNA was established and their anticancer activity was shown.
Subject(s)
DNA , Polydeoxyribonucleotides , BiotechnologyABSTRACT
We studied antidepressant and antiparkinsonian properties of N-(5-hydroxynicotinoyl)-Lglutamic acid calcium salt (Ampasse) in rodents. It was found that Ampasse in a dose of 30 mg/kg exhibited antidepressant activity in the forced swimming test in mice and in a dose of 0.1 mg/kg maximally alleviates the symptoms of parkinsonian syndrome induced by systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine in C57Bl/6 mice, and haloperidol-induced catalepsy in rats.
Subject(s)
Antidepressive Agents/therapeutic use , Calcium/chemistry , Depression/drug therapy , Glutamic Acid/chemistry , Glutamic Acid/therapeutic use , Parkinsonian Disorders/drug therapy , Animals , Catalepsy/chemically induced , Haloperidol/pharmacology , Male , Mice , Mice, Inbred C57BLABSTRACT
Dose-dependent protective effects of lanthanum nitrate solution and gel were shown on the model of experimental infection caused by a virulent strain of Shigella flexneri 2a or opportunistic bacteria Klebsiella pneumoniae in outbred and DBA mice.
Subject(s)
Lanthanum/pharmacology , Animals , Klebsiella pneumoniae/drug effects , Klebsiella pneumoniae/pathogenicity , Mice , Mice, Inbred DBA , Shigella flexneri/drug effects , Shigella flexneri/pathogenicity , Simplexvirus/drug effects , Simplexvirus/pathogenicityABSTRACT
The inhibitory effect of D and L-polynucleotides of a given length (40-50n) on the catalytic activity of DNA polymerase ß isolated from chromatin cells of acute myeloid leukemia HL-60 was evaluated. The synthesized L enantiomer was found to have a higher inhibitory activity than the synthesized and isolated D enantiomers of polynucleotides. The work also proposes a biophysical model that describes this effect.