ABSTRACT
The researches on the identification of gibberellin receptor are reviewed from the early attempts in 1960s to the identification of GIBBERELLIN INSENSITIVE DWARF1 (GID1) as the receptor in 2005. Unpublished data of the gibberellin-binding protein in the seedlings of adzuki bean (Vigna angularis) are also included, suggesting that the active principle of the gibberellin-binding protein was a GID1 homolog.
Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Carrier Proteins/metabolism , Gene Expression Regulation, Plant , Gibberellins/metabolism , Phosphoproteins/metabolism , Plant Growth Regulators/metabolism , Plant Proteins/metabolism , Receptors, Cell Surface/metabolism , Vigna/metabolism , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Carrier Proteins/genetics , Cell Membrane/metabolism , Phosphoproteins/genetics , Plant Cells/metabolism , Plant Proteins/genetics , Protein Binding , Receptors, Cell Surface/genetics , Seedlings/genetics , Seedlings/metabolism , Signal Transduction , Vigna/geneticsABSTRACT
Auxin is a fundamental plant hormone and its localization within organs plays pivotal roles in plant growth and development. Analysis of many Arabidopsis mutants that were defective in auxin biosynthesis revealed that the indole-3-pyruvic acid (IPA) pathway, catalyzed by the TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) and YUCCA (YUC) families, is the major biosynthetic pathway of indole-3-acetic acid (IAA). In contrast, little information is known about the molecular mechanisms of auxin biosynthesis in rice. In this study, we identified a auxin-related rice mutant, fish bone (fib). FIB encodes an orthologue of TAA genes and loss of FIB function resulted in pleiotropic abnormal phenotypes, such as small leaves with large lamina joint angles, abnormal vascular development, small panicles, abnormal organ identity and defects in root development, together with a reduction in internal IAA levels. Moreover, we found that auxin sensitivity and polar transport activity were altered in the fib mutant. From these results, we suggest that FIB plays a pivotal role in IAA biosynthesis in rice and that auxin biosynthesis, transport and sensitivity are closely interrelated.
Subject(s)
Indoleacetic Acids/metabolism , Oryza/genetics , Plant Growth Regulators/metabolism , Plant Proteins/physiology , Tryptophan Transaminase/physiology , Amino Acid Sequence , Biological Transport/genetics , Cloning, Molecular , Molecular Sequence Data , Oryza/enzymology , Oryza/metabolism , Phenotype , Plant Proteins/chemistry , Plant Proteins/genetics , Sequence Alignment , Sequence Analysis, Protein , Tryptophan Transaminase/chemistry , Tryptophan Transaminase/geneticsABSTRACT
Gibberellins (GAs) are phytohormones that are essential for many developmental processes in plants. It has been postulated that plants have both membrane-bound and soluble GA receptors; however, no GA receptors have yet been identified. Here we report the isolation and characterization of a new GA-insensitive dwarf mutant of rice, gid1. The GID1 gene encodes an unknown protein with similarity to the hormone-sensitive lipases, and we observed preferential localization of a GID1-green fluorescent protein (GFP) signal in nuclei. Recombinant glutathione S-transferase (GST)-GID1 had a high affinity only for biologically active GAs, whereas mutated GST-GID1 corresponding to three gid1 alleles had no GA-binding affinity. The dissociation constant for GA4 was estimated to be around 10(-7) M, enough to account for the GA dependency of shoot elongation. Moreover, GID1 bound to SLR1, a rice DELLA protein, in a GA-dependent manner in yeast cells. GID1 overexpression resulted in a GA-hypersensitive phenotype. Together, our results indicate that GID1 is a soluble receptor mediating GA signalling in rice.
Subject(s)
Gibberellins/metabolism , Oryza/genetics , Oryza/metabolism , Plant Proteins/metabolism , Protein Binding , Amino Acid Sequence , Binding, Competitive , Cloning, Molecular , Epistasis, Genetic , Genes, Plant/genetics , Gibberellins/pharmacology , Molecular Sequence Data , Mutation/genetics , Oryza/drug effects , Phenotype , Plant Proteins/chemistry , Plant Proteins/genetics , Solubility , Sterol Esterase/chemistryABSTRACT
In Arabidopsis, three receptors exist for the phytohormone gibberellin. Of the three, only a double loss-of-function mutant (atgid1a atgid1c) shows a dwarf phenotype, while other double and all single mutants show no abnormality in height. In this study we show that the expression of AtGID1b-GUS mRNA, driven by the AtGID1b promoter, is low in inflorescence stems, but may be 10% of AtGID1a-GUS mRNA, driven by the AtGID1a promoter. However, AtGID1b-GUS enzymatic activity does not exist in them. This factor strongly suggests that atgid1a atgid1c lacks sufficient AtGID1b protein for normal stem growth. In the stamens of pAtGID1c::AtGID1c-GUS transformants, we detected clear AtGID1c-GUS activity, while another atgid1a atgid1b, which has short stamens in its flowers, causes the adhesion of little pollen to stigmas thus leading to its low fertility. We then evaluated the affinity of the AtGID1-DELLA interaction by a competitive yeast three-hybrid system and also by QCM apparatus. AtGID1c showed a quite lower affinity to RGL2, the major DELLA protein in floral buds, than AtGID1a or AtGID1b. The low affinity of the AtGID1c-RGL2 interaction is likely to be responsible for the failure of AtGID1c to hold RGL2, which is required for normal stamen development. Taken together with expressional information of DELLA genes, we propose that in a double loss-of-function mutant of gibberellin receptors, the emergence of any phenotype(s) depends on the abundance of the remaining receptor and its preference to DELLA proteins existing at a target site.
Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/genetics , Gibberellins/metabolism , Plant Growth Regulators/metabolism , Receptors, Cell Surface/metabolism , Arabidopsis/growth & development , Arabidopsis Proteins/genetics , Gene Expression Regulation, Plant , Mutation , Phenotype , Plants, Genetically Modified/genetics , Plants, Genetically Modified/growth & development , Protein Binding , RNA, Plant/genetics , Receptors, Cell Surface/genetics , Signal TransductionABSTRACT
A highly sensitive quantitative method for assaying nicotianamine (NA) and 2'-deoxymugineic acid (DMA) using liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) was developed. The amino and hydroxyl groups of NA and DMA were derivatized using 9-fluorenylmethoxycarbonyl chloride. The amounts of NA and DMA in 10 mul of xylem sap from rice cultivated under iron (Fe)-sufficient and Fe-deficient conditions were quantified without concentration. In Fe-sufficient plants, the concentrations of NA and DMA were almost equal to that of Fe. In Fe-deficient plants, the concentration of NA did not change significantly, whereas that of DMA increased markedly.
Subject(s)
Azetidinecarboxylic Acid/analogs & derivatives , Chromatography, Liquid/methods , Oryza/chemistry , Spectrometry, Mass, Electrospray Ionization/methods , Azetidinecarboxylic Acid/analysis , Iron/metabolism , Xylem/chemistryABSTRACT
Arabinogalactan proteins (AGPs) are hydroxyproline-rich glycoproteins present at the plasma membrane and in extracellular spaces. A synthetic chemical, beta-glucosyl Yariv reagent (beta-GlcY), binds specifically to AGPs. We previously reported that gibberellin signaling is specifically inhibited by beta-GlcY treatment in barley aleurone protoplasts. In the present study, we found that beta-GlcY also inhibited gibberellin-induced programmed cell death (PCD) in aleurone cells. We examined the universality and specificity of the inhibitory effect of beta-GlcY on gibberellin signaling using microarray analysis and found that beta-GlcY was largely effective in repressing gibberellin-induced gene expression. In addition, >100 genes were up-regulated by beta-GlcY in a gibberellin-independent manner, and many of these were categorized as defense-related genes. Defense signaling triggered by several defense system inducers such as jasmonic acid and a chitin elicitor could inhibit gibberellin-inducible events such as alpha-amylase secretion, PCD and expression of some gibberellin-inducible genes in aleurone cells. Furthermore, beta-GlcY repressed the gibberellin-inducible Ca2+-ATPase gene which is important for gibberellin-dependent gene expression, and induced known repressors of gibberellin signaling, two WRKY genes and a NAK kinase gene. These effects of beta-GlcY were also phenocopied by the chitin elicitor and/or jasmonic acid. These results indicate that gibberellin signaling is under the regulation of defense-related signaling in aleurone cells. It is also probable that AGPs are involved in the perception of stimuli causing defense responses.
Subject(s)
Galactans/metabolism , Gibberellins/antagonists & inhibitors , Glucosides/pharmacology , Hordeum/cytology , Hordeum/metabolism , Phloroglucinol/analogs & derivatives , Signal Transduction/drug effects , Cyclopentanes/metabolism , Gene Expression Profiling , Gene Expression Regulation, Plant , Gibberellins/pharmacology , Glucosides/metabolism , Hordeum/drug effects , Oxylipins/metabolism , Phloroglucinol/metabolism , Phloroglucinol/pharmacology , Plant Proteins/genetics , Plant Proteins/metabolism , Protein Array AnalysisABSTRACT
As a new detection method of bioactive gibberellin A(4) (GA(4)) in living cells, a combined system of GA(4)-dependent interaction of V(H) and V(L) composed of a variable region fragment (Fv) of anti-GA(4) antibodies and protein-fragment complementation assay (PCA) was developed. First, when V(H) and V(L) were displayed in proximity on a phage, they could constitute a functional Fv. Thereafter, V(H) and V(L) were shown to interact with each other in a GA(4)-dependent manner. We then applied this interaction to PCA using GFP as a reporter. V(H) fused to the C-terminal half of GFP and V(L) fused to the N-terminal half of GFP were simultaneously expressed in Escherichia coli. The E. coli in which these fusion proteins were inductively produced in the presence of GA(4) showed clear GFP fluorescence, while those in the absence of GA(4) showed only scarce GFP fluorescence, demonstrating the feasibility of this system to detect GA(4) in living organisms.
Subject(s)
Antibodies/immunology , Enzyme-Linked Immunosorbent Assay/methods , Gibberellins/analysis , Immunoglobulin Heavy Chains/immunology , Immunoglobulin Light Chains/immunology , Immunoglobulin Variable Region/metabolism , Antibodies/genetics , Escherichia coli/genetics , Gibberellins/immunology , Green Fluorescent Proteins/analysis , Green Fluorescent Proteins/genetics , Immunoglobulin Heavy Chains/genetics , Immunoglobulin Light Chains/genetics , Immunoglobulin Variable Region/genetics , Peptide Library , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/immunologyABSTRACT
Immunomodulation is a means to modulate an organism's function by antibody production to capture either endogenous or exogenous antigens. This method was applied to plants to repress the function of gibberellins (GAs), a class of phytohormones responsible for plant elongation, by anti-bioactive GA antibodies. Two different antibodies were produced in Arabidopsis as single-chain variable fragment (scFv) fused to green fluorescent protein (GFP) with four different subcellular localizations: endoplasmic reticulum (ER), cytosol, apoplastic space or the outer surface of the plasma membrane. When targeting scFv-GFP to ER, plants showed the highest accumulation of scFv-GFP, with binding activity, strong GFP fluorescence in ER-derived compartments and mild but clear GA-deficient phenotypes, including a smaller leaf size, delayed bolting, shorter inflorescence length and decreased germination. Plants expressing scFv-GFP in ER responded to exogenous GA(4) and contained 15-40 times greater endogenous GA(4) than wild-type plants. They also showed increased gene expression for GA3ox1, GA20ox1 and GA20ox2, but decreased expression for GA2ox1, which are feedback and feedforward regulated by GA signalling, respectively. These results suggest that the level of free functional GA(4) decreased when trapped in the ER with scFv to the extent that mild GA-deficient phenotypes were created. A dramatic increase in the total sum of GA(4) (free plus scFv-GFP bound) was detected as a result of the up-regulation of GA biosynthesis (feedback regulated), and a decrease in GA(4) catabolism as a result of protection by scFv-GFP binding. This study demonstrates that the use of immunomodulation to inhibit the action of bioactive GAs is an effective method of creating GA-deficient plants.
Subject(s)
Arabidopsis/immunology , Arabidopsis/metabolism , Gene Expression Regulation, Plant/immunology , Gibberellins/immunology , Gibberellins/metabolism , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Gibberellins/chemistry , Immunoglobulin Variable Region/immunology , Molecular Structure , Phenotype , Plant Epidermis/cytology , Plant Epidermis/metabolism , Plant Leaves/cytology , Plant Leaves/metabolism , Plants, Genetically Modified , Recombinant ProteinsABSTRACT
Using NMR spectroscopy and simulated annealing calculations, we determined the solution structure of the disulfide-linked cyclized decapeptide ACLPWSDGPC (SD), which is bound to an anti-(gibberellin A(4)) mAb 4-B8(8)/E9 and was found to be the first peptidyl mimotope for a hydrophobic ligand. The resulting structure of the peptide showed a beta-turn-like conformation in residues three to seven and the region converges well (average rmsd 0.54 A). The binding activity and the epitopes of the peptide to the antibody were assessed using saturation transfer difference (STD)-NMR experiments. We also conducted docking simulations between the peptide and the mAb to determine how the peptide is bound to the mAb. Resonances around the beta-turn-like conformation of peptide SD (residues 3-5) showed strong STD enhancement, which agreed well with results from docking simulation between peptide SD and the mAb. Together with the commonality of amino acid residues of the mAb involved in interactions with gibberellin A(4) (GA(4)) and peptide SD, we concluded that peptide SD is bound to the antigen-binding site of mAb 4-B8(8)/E9 as a GA(4) mimic, confirming evidence for the existence of peptide mimics even for hydrophobic ligands.
Subject(s)
Biomimetics , Computer Simulation , Epitope Mapping , Gibberellins/chemistry , Peptides, Cyclic/chemistry , Peptides, Cyclic/immunology , Amino Acid Sequence , Antibodies, Monoclonal/immunology , Hydrophobic and Hydrophilic Interactions , Ligands , Magnetic Resonance Spectroscopy , Models, Molecular , Molecular Sequence Data , Molecular Structure , Peptides, Cyclic/chemical synthesis , Protein Structure, TertiaryABSTRACT
An anti-chlorpropham single-chain variable-fragment (scFv) gene was introduced into Arabidopsis in a manner to express the antibody fragment in each of four different subcellular compartments. The accumulation of scFv in transgenic plants was detected by targeting the fragment in the endoplasmic reticulum or apoplastic space, or by expressing the fragment as a glycosylphosphatidylinositol-anchored protein, while no accumulation could be detected by targeting the fragment in the cytosol. Transgenic plants accumulating the scFv gene at a high level in the endoplasmic reticulum had enhanced tolerance to chlorpropham in comparison with the non-transformants.
Subject(s)
Antibodies/genetics , Arabidopsis/physiology , Chlorpropham/pharmacology , Drug Resistance/genetics , Herbicides/pharmacology , Plants, Genetically Modified/physiology , Arabidopsis/drug effects , Arabidopsis/genetics , Genetic Vectors , Immunoglobulin Variable Region/genetics , Plants, Genetically Modified/drug effects , Plants, Genetically Modified/geneticsABSTRACT
Immunomodulation is a means to modulate an organism's function by antibody production to capture either endogenous or exogenous antigens. We have recently succeeded in obtaining gibberellin (GA)-deficient phenotypes in Arabidopsis thaliana by using anti-bioactive GA antibodies. In this study, a single-chain antibody (scFv) against GA(24), a precursor GA, was utilized to repress the biosynthesis of bioactive gibberellins. Stable accumulation of the scFv in endoplasmic reticulum (ER) was achieved by being produced as a fusion with GFP as well as KDEL ER-retention signal. The transgenic plants showed GFP fluorescence in the reticulate cortical ER network in epidermal cells. The GFP-scFv fusion produced in plants maintained its binding activity. The transgenic plants showed GA-deficient phenotypes, including reduced rosette leaf development, delayed flower induction and reduced stem elongation of the main culm, especially in the early stage of inflorescence growth. Contrarily, stem elongation of the main culm at a later stage, or that of lateral shoots was much less affected by scFv production. These phenotypes were different from anti-bioactive GA scFv-producing lines, whose stem elongation was continuously repressed throughout the inflorescence development. The GA-deficient phenotypes were recovered by treatment with GA(24) and bioactive GA(4), the latter being more effective. The transgenic lines contained conspicuously higher endogenous GA(24) and clearly less GA(4) than wild-type plants. The expression of GA 20-oxidase and GA 3-oxidase genes, which are feedback-regulated by GA signaling, were up-regulated in those plants. These results demonstrate that the scFv trapped GA(24) in ER and inhibited metabolism of GA(24) to bioactive GA(4).
Subject(s)
Antibodies/metabolism , Arabidopsis/metabolism , Gibberellins/metabolism , Plants, Genetically Modified/metabolism , Antibodies/genetics , Antibodies/immunology , Arabidopsis/genetics , Arabidopsis/growth & development , Endoplasmic Reticulum/metabolism , Gene Expression Regulation, Developmental , Gene Expression Regulation, Plant , Gibberellins/immunology , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Phenotype , Plants, Genetically Modified/genetics , Plants, Genetically Modified/growth & development , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
A glucosyltransferase gene InGTase1 was identified from the immature seeds of morning glory (Ipomoea nil), whose product shows a broad substrate-preference, including that of some phytohormones. When 2-trans-abscisic acid, indole-3-acetic acid, salicylic acid (SA) or (+/-)-jasmonic acid was reacted with InGTase1 and UDP-[(14)C]-glucose, each (14)C-labeled compound with high polarity was detected after thin layer chromatography. SA metabolites were identified as SA glucosyl ester by using (1)H NMR and GC/MS. Detailed substrate-preferences of InGTase1 were examined with some analogous compounds, which elucidated that the arm length and/or orientation of a carboxyl group of the compounds or its surrounding electron density severely affected the enzymatic activity. The broad substrate-preference will greatly contribute to the synthesis of various glucoconjugates.
Subject(s)
Glucosyltransferases/metabolism , Ipomoea nil/enzymology , Plant Growth Regulators/metabolism , Plant Proteins/metabolism , Base Sequence , Cloning, Molecular , Glucosyltransferases/genetics , Molecular Sequence Data , Plant Growth Regulators/chemistry , Plant Proteins/genetics , Sequence Alignment , Substrate Specificity , Uridine Diphosphate Glucose/metabolismABSTRACT
A highly sensitive quantitative method for analyzing nicotianamine (NA) by liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) is reported. Fluorenylmethoxycarbonylation of nicotianamine reduced its polarity and enabled its retention in a reversed-phase column. The adoption of N(epsilon)-nicotyllysine (NL) as an internal standard ensured reliable quantification by giving a linear calibration curve drawn between the NA/NL molar ratios of standard solutions injected and the NA/NL area ratios in mass chromatograms. The high sensitivity of this analytical method allowed us to measure the amount of NA. This analytical method has applications to all research concerning NA.
Subject(s)
Angiotensin-Converting Enzyme Inhibitors/analysis , Azetidinecarboxylic Acid/analogs & derivatives , Azetidinecarboxylic Acid/analysis , Calibration , Chromatography, High Pressure Liquid , Indicators and Reagents , Oryza/chemistry , Plant Leaves/chemistry , Reference Standards , Reproducibility of Results , Seeds/chemistry , Spectrometry, Mass, Electrospray Ionization , Nicotiana/chemistryABSTRACT
Arabidopsis carries three receptor genes for the phytohormone gibberellin (GA), AtGID1a, AtGID1b and AtGID1c. Expression of each gene in the rice gid1-1 mutant for GA receptors causes reversion of its severely dwarfed phenotype and GA insensitivity to a normal level, even though each loss-of-function mutant shows no clear phenotype in Arabidopsis (Nakajima et al., 2006). In this paper, we report the functional redundancy and specificity of each AtGID1 by analyzing the multiple mutants for loss of function. Seeds of the double knockout mutants atgid1a atgid1b, atgid1a atgid1c and atgid1b atgid1c germinated normally. The double knockout mutant atgid1a atgid1c showed a dwarf phenotype, while other double mutants were of normal height compared to the wild-type. The stamens of the double knockout mutant atgid1a atgid1b were significantly shorter than those of the wild-type, and this leads to low fertility. A severe disarrangement of the pattern on its seed surface was also observed. The triple knockout mutant atgid1a atgid1b atgid1c did not germinate voluntarily, and only started to grow when the seed coat was peeled off after soaking. Seedlings of the triple knockout mutants were severe dwarfs, only a few millimeters high after growing for 1 month. Moreover, the triple knockout seedlings completely lost their ability to respond to exogenously applied GA. These results show that all AtGID1s function as GA receptors in Arabidopsis, but have specific role(s) for growth and development.
Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Gibberellins/metabolism , Receptors, Cell Surface/metabolism , Arabidopsis/genetics , Arabidopsis/growth & development , Arabidopsis Proteins/genetics , DNA, Bacterial , Germination/physiology , Mutagenesis, Insertional , Phenotype , Receptors, Cell Surface/genetics , Signal Transduction/physiologyABSTRACT
GIBBERELLIN INSENSITIVE DWARF1 (GID1) encodes a soluble gibberellin (GA) receptor that shares sequence similarity with a hormone-sensitive lipase (HSL). Previously, a yeast two-hybrid (Y2H) assay revealed that the GID1-GA complex directly interacts with SLENDER RICE1 (SLR1), a DELLA repressor protein in GA signaling. Here, we demonstrated, by pull-down and bimolecular fluorescence complementation (BiFC) experiments, that the GA-dependent GID1-SLR1 interaction also occurs in planta. GA(4) was found to have the highest affinity to GID1 in Y2H assays and is the most effective form of GA in planta. Domain analyses of SLR1 using Y2H, gel filtration, and BiFC methods revealed that the DELLA and TVHYNP domains of SLR1 are required for the GID1-SLR1 interaction. To identify the important regions of GID1 for GA and SLR1 interactions, we used many different mutant versions of GID1, such as the spontaneous mutant GID1s, N- and C-terminal truncated GID1s, and mutagenized GID1 proteins with conserved amino acids replaced with Ala. The amino acid residues important for SLR1 interaction completely overlapped the residues required for GA binding that were scattered throughout the GID1 molecule. When we plotted these residues on the GID1 structure predicted by analogy with HSL tertiary structure, many residues were located at regions corresponding to the substrate binding pocket and lid. Furthermore, the GA-GID1 interaction was stabilized by SLR1. Based on these observations, we proposed a molecular model for interaction between GA, GID1, and SLR1.
Subject(s)
Gibberellins/metabolism , Oryza/metabolism , Plant Proteins/metabolism , Receptors, Cell Surface/metabolism , Alleles , Amino Acid Sequence , Dose-Response Relationship, Drug , Gibberellins/pharmacology , Models, Biological , Molecular Sequence Data , Mutagenesis , Oryza/drug effects , Plant Leaves/drug effects , Plant Leaves/metabolism , Plant Proteins/chemistry , Protein Binding/drug effects , Protein Structure, Secondary , Protein Structure, Tertiary , Receptors, Cell Surface/chemistry , Saccharomyces cerevisiae/drug effects , Solubility/drug effects , Structure-Activity Relationship , Two-Hybrid System TechniquesABSTRACT
Previously we reported the first example of peptide mimics of a small hydrophobic molecule, a phytohormone gibberellin. The second peptide mimic of gibberellin has been identified from random peptide libraries by its affinity to a type of catalyzing enzyme of gibberellins, which specifically recognizes bioactive gibberellins. These results suggest that even hydrophobic compounds can be mimicked by peptides.
Subject(s)
Gibberellins/chemistry , Mixed Function Oxygenases/chemistry , Peptides/chemistry , Catalysis , Hydrophobic and Hydrophilic Interactions , Molecular Mimicry , Peptide Library , Structure-Activity RelationshipABSTRACT
Transcripts of thionin genes encoding antimicrobial peptides were present at a high level in rice coleoptiles just after germination, and decreased to an undetectable level after about 3 d, but this decline was suppressed by co-treatment with gibberellic acid (GA(3)) and brassinolide (BL). The temporal expression patterns of key enzyme genes for the biosyntheses of gibberellins (GAs) and brassinosteroids (BRs) were correlated with the fluctuation of thionin mRNAs. Jasmonic acid (JA) replaced the effect of GA3 and BL, and its change in endogenous level was parallel to that of the thionin genes. These results strongly suggest that thionin gene expression was positively regulated by JA, whose endogenous level was synergistically regulated by GAs and BRs. In contrast, thionin gene expression in etiolated seedlings remained high while the endogenous level of JA was low, suggesting the presence of another signaling pathway in the dark to maintain the thionin level.
Subject(s)
Antimicrobial Cationic Peptides/genetics , Cholestanols/metabolism , Gene Expression Regulation, Plant , Gibberellins/biosynthesis , Plant Proteins/genetics , Steroids, Heterocyclic/metabolism , Brassinosteroids , Cholestanols/pharmacology , Cyclopentanes/pharmacology , Gene Expression Regulation, Plant/drug effects , Genes, Plant , Gibberellins/pharmacology , Oryza , Oxylipins , RNA, Messenger , Seedlings/genetics , Steroids, Heterocyclic/pharmacologyABSTRACT
Three gibberellin (GA) receptor genes (AtGID1a, AtGID1b and AtGID1c), each an ortholog of the rice GA receptor gene (OsGID1), were cloned from Arabidopsis, and the characteristics of their recombinant proteins were examined. The GA-binding activities of the three recombinant proteins were confirmed by an in vitro assay. Biochemical analyses revealed similar ligand selectivity among the recombinants, and all recombinants showed higher affinity to GA(4) than to other GAs. AtGID1b was unique in its binding affinity to GA(4) and in its pH dependence when compared with the other two, by only showing binding in a narrow pH range (pH 6.4-7.5) with 10-fold higher affinity (apparent K(d) for GA(4) = 3 x 10(-8) m) than AtGID1a and AtGID1c. A two-hybrid yeast system only showed in vivo interaction in the presence of GA(4) between each AtGID1 and the Arabidopsis DELLA proteins (AtDELLAs), negative regulators of GA signaling. For this interaction with AtDELLAs, AtGID1b required only one-tenth of the amount of GA(4) that was necessary for interaction between the other AtGID1s and AtDELLAs, reflecting its lower K(d) value. AtDELLA boosted the GA-binding activity of AtGID1 in vitro, which suggests the formation of a complex between AtDELLA and AtGID1-GA that binds AtGID1 to GA more tightly. The expression of each AtGID1 clone in the rice gid1-1 mutant rescued the GA-insensitive dwarf phenotype. These results demonstrate that all three AtGID1s functioned as GA receptors in Arabidopsis.
Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/metabolism , Gibberellins/metabolism , Receptors, Cell Surface/genetics , Amino Acid Sequence , Arabidopsis/genetics , Arabidopsis Proteins/chemistry , Arabidopsis Proteins/metabolism , Genetic Complementation Test , Molecular Sequence Data , Oryza/anatomy & histology , Oryza/genetics , Phylogeny , Plants, Genetically Modified/anatomy & histology , Plants, Genetically Modified/metabolism , Receptors, Cell Surface/chemistry , Receptors, Cell Surface/metabolism , Recombinant Fusion Proteins/metabolism , Sequence Alignment , Two-Hybrid System TechniquesABSTRACT
Gibberellins A1/3 (GA1/3) and GA20 appeared earlier in surrounding tissues (pericarps/carpel/placenta) than in developing seeds of morning glory. The content of GA1/3 became higher in seeds than in the surrounding tissues at 9 days after anthesis (DAA), while that of GA20 stayed lower in seeds even at 12 DAA, suggesting the possibility that GA20 was translocated into seeds from the surrounding tissues and converted to GA1/3. The site of biosynthesis of GA20 in the fruits was determined by RNA-blotting and in situ hybridization of GA 20-oxidase genes (InGA20ox1, InGA20ox2). InGA20ox1 was not expressed in the surrounding tissues but in seeds, while no signal due to InGA20ox2 was detected in neither tissue. The expression of InGA20ox1 started in the seed coat near the hilum and spread in the seed coat like those of GA 3-oxidase and GA-inducible alpha-amylase genes. These observations suggest that GA biosynthesis is tissue-specifically and time-dependently regulated in the fruit of morning glory.
Subject(s)
Gene Expression Regulation, Developmental/physiology , Gene Expression Regulation, Plant/physiology , Gibberellins/metabolism , Ipomoea/metabolism , Mixed Function Oxygenases/metabolism , Fruit/growth & development , Fruit/metabolism , Gene Expression Profiling , Gibberellins/chemistry , Ipomoea/enzymology , Ipomoea/growth & development , Molecular Structure , Seeds/enzymologyABSTRACT
To clarify the role of gibberellins in the seed development of Arabidopsis, we investigated the sites where gibberellins are synthesized and induce alpha-amylase genes. The spatial and temporal expression of the genes encoding gibberellin biosynthetic enzymes and alpha-amylases was examined by reverse transcription-PCR (RT-PCR) and in situ hybridization. The mRNAs of AtGA20ox2, AtGA20ox3 and AtGA3ox4 began to be detectable 5-7 d after pollination. In situ hybridization showed that these genes were expressed almost simultaneously around starch granules in the outer integument, preceding the disappearance of those granules. AtGA20ox2 and AtGA3ox4 but not AtGA20ox3 also showed their signals at the rim of the developing embryo. The alpha-amylase gene, Amy3, which responded to gibberellin, was mainly expressed in the developing seed, spatially overlapping with the expression of AtGA20ox2 and AtGA3ox4. These results suggest that gibberellins function in at least two sites of the seed: the outer integument and part of the embryo. We examined the phenotypes of a T-DNA insertion line of AtGA3ox4 and observed the following: (i) a decrease of alpha-amylase gene transcripts in young siliques; (ii) delay of starch degradation in the outer integument; (iii) disarrangement of the seed surface structure; and (iv) abnormal swelling pattern of polysaccharides after imbibition by the mature seed. These characteristics are phenotypes of plants under gibberellin starvation, because the abnormalities could be almost overcome with applied gibberellin, and the gibberellin-treated mutant was indistinguishable from the wild type. These results strongly suggest that gibberellins in the outer integument would be required for the normal formation of the Arabidopsis seed coat.