The effect of MicroRNAs variants on idiopathic recurrent pregnancy loss.

BACKGROUND: Although the importance of miRNA variants in female reproductive disorders has been frequently reported, the association between miRNA polymorphisms and recurrent pregnancy loss (RPL) has been poorly studied. In this study, we aimed to assess the correlation of four different miRNA variants to unexplained RPL. METHODS AND RESULTS: The prevalence of four SNPs including miR-21 rs1292037, miR-155-5p rs767649, miR-218-2 rs11134527, and miR-605 rs2043556 in 280 cases with iRPL and 280 controls was performed. The DNA was extracted from all subjects and the SNPs were genotyped using RFLP-PCR methods. The data revealed that rs1292037 and rs767649 were significantly associated with higher rates of iRPL in patients compared with controls while rs11134527 and rs2043556 showed no association with increased rates of iRPL among patients. The haplotypes T-A-G-G and T-A-G-A were the most frequent in both cases and controls. Three haplotypes including T-T-G-A, C-T-G-G, and T-A-A-A showed significantly different frequencies in patients in comparison to healthy females. CONCLUSION: This study suggests that rs1292037 and rs767649 could be risk factors for increased rates of iRPL.

Combined detection of ACL and ß2-GP1 in diagnosis of adverse pregnancy.

OBJECTIVE: To illustrate the significance of combined detection of anti cardiolipin (ACL) and anti-ß2-glycoprotein 1 (ß2-GP1) in the diagnosis of adverse pregnancy. METHODS: 60 postnatal women with adverse pregnancy presented to our hospital from March 2019 to March 2020 were enrolled as the experimental group, and 60 postnatal women with normal delivery in the same period were selected as the control group. The levels of ACL, ß2-GP1 were measured to analyze the significance of the integrated detection in the diagnosis of adverse pregnancy. RESULTS: No obvious differences were observed in mean age, mean gestational week, mean height, number of cases of primigravida and multiparous women, educational background, place of residence between two groups (P > 0.05). The number of cases of positive ß2-GP1-IgG and ß2-GP1-IgM tests in experimental group were remarkably higher (P < 0.05). The number of ß2-GP1-IgA positive cases in the two kinds of parturients was not notably different (P > 0.05). The ACL of the experimental parturients had a notably higher number of ß2-GP1-positive cases (P < 0.001), and the combined positive cases of the experimental parturients had notably higher serum BPA and resistin levels (P < 0.001). The incidence of fetal growth restriction, premature delivery and placental abruption of experimental group were higher than those of control group (P < 0.05). There was no stillbirth in both groups. CONCLUSIONS: The positive expression of ACL with ß2-GP1 is the culprit of adverse pregnancy, and the combined diagnosis can greatly improve the clinical screening rate of adverse pregnancy and provide more evidence for subsequent treatment.