The mechanism of abscisic acid regulation of wild Fragaria species in response to cold stress.

BACKGROUND: Abiotic stresses have increasingly serious effects on the growth and yield of crops. Cold stress, in particular, is an increasing problem. In this study, Fragaria daltoniana and F. vesca were determined to be cold-resistant and cold-sensitive species, respectively. Integrated transcriptomics and metabolomics methods were used to analyze the regulatory mechanism of abscisic acid (ABA) in F. daltoniana and F. vesca in their response to low temperature stress. RESULTS: F. daltoniana and F. vesca increased their ABA content under low temperature stress by upregulating the expression of the ABA biosynthetic pathway gene NCED and downregulating the expression of the ABA degradative gene CYP707A. Both types of regulation increased the accumulation of glucose and fructose, resulting in a reduction of damage under low temperature stress. Twelve transcription factors were found to be involved in the ABA regulatory pathway. The strong cold tolerance of F. daltoniana could be owing to its higher levels of ABA that accumulated compared with those in F. vesca under low temperature stress. In addition, the gene ABF2, which is related to the transduction of glucose signaling, was significantly upregulated in the leaves of F. daltoniana, while it was downregulated in the leaves of F. vesca under low temperature stress. This could contribute to the higher levels of glucose signal transduction in F. daltoniana. Thus, this could explain the higher peroxidase activity and lower damage to cell membranes in the leaves of F. daltoniana compared with F. vesca under low temperature stress, which endows the former with stronger cold tolerance. CONCLUSIONS: Under low temperature stress, the differences in the accumulation of ABA and the expression trends of ABF2 and ABF4 in different species of wild strawberries may be the primary reason for their differences in cold tolerance. Our results provide an important empirical reference and technical support for breeding resistant cultivated strawberry plants.

Older populations of the invader Solidago canadensis exhibit stronger positive plant-soil feedbacks and competitive ability in China.

PREMISE: The enemy release hypothesis predicts that release from natural enemies, including soil-borne pathogens, liberates invasive plants from a negative regulating force. Nevertheless, invasive plants may acquire novel enemies and mutualists in the introduced range, which may cause variable effects on invader growth. However, how soil microorganisms may influence competitive ability of invasive plants along invasion chronosequences has been little explored. METHODS: Using the invasive plant Solidago canadensis, we tested whether longer residence times are associated with stronger negative plant-soil feedbacks and thus weaker competitive abilities at the individual level. We grew S. canadensis individuals from 36 populations with different residence times across southeastern China in competition versus no competition and in three different types of soils: (1) conspecific rhizospheric soils; (2) soils from uninvaded patches; and (3) sterilized soil. For our competitor treatments, we constructed synthetic communities of four native species (Bidens parviflora, Solanum nigrum, Kalimeris indica, and Mosla scabra), which naturally co-occur with Solidago canadensis in the field. RESULTS: Solidago canadensis populations with longer residence times experienced stronger positive plant-soil feedbacks and had greater competitive responses (i.e., produced greater above-ground biomass and grew taller) in conspecific rhizospheric soils than in sterilized or uninvaded soils. Moreover, S. canadensis from older populations significantly suppressed above-ground biomass of the native communities in rhizospheric and uninvaded soils but not in sterilized soil. CONCLUSIONS: The present results suggest that older populations of S. canadensis experience stronger positive plant-soil feedbacks, which may enhance their competitive ability against native plant communities.

The invasive plant Solidago canadensis exhibits partial local adaptation to low salinity at germination but not at later life-history stages.

PREMISE: Evolutionary adaptation may enable plants to inhabit a broad range of environments. However, germination and early life-history stages have seldom been considered in estimates of evolutionary adaptation. Moreover, whether soil microbial communities can influence evolutionary adaptation in plants remains little explored. METHODS: We used reciprocal transplant experiments to investigate whether two populations of an invasive plant Solidago canadensis that occur in contrasting habitats of low versus high salinity expressed adaptation to the respective salinity levels. We germinated S. canadensis seeds collected from low-and high-salinity habitats under low- and high-salt treatments. We also raised S. canadensis seedlings from the two salinity habitats under low- and high-salt treatments and in the presence versus absence of microbial communities from the two habitats. RESULTS: Genotypes from a low-salinity habitat had higher germination rates under low-salt treatment than genotypes from a high-salinity habitat. However, both genotypes had similar germination rates under a high-salt treatment. The two genotypes also had similar seedling survival and biomass responses to low- and high-salt treatments. Nevertheless, seedling biomass was significantly higher under low salt treatment. Soil microbial communities did not influence biomass of S. canadensis under the two salt treatments. CONCLUSIONS: The results on germination rates suggest partial local adaptation to low salinity. However, there was no evidence of local adaptation to salinity at the seedling survival and growth stages. The finding that germination and seedling biomass responded to different salt treatments suggests that the two traits are important for salt tolerance.

Common mycorrhizal networks influence the distribution of mineral nutrients between an invasive plant, Solidago canadensis, and a native plant, Kummerowa striata.

Invasive species often reduce ecosystem services and lead to a serious threat to native biodiversity. Roots of invasive plants are often linked to roots of native plants by common mycorrhizal networks (CMNs) of arbuscular mycorrhizal (AM) fungi, but whether and how CMNs mediate interactions between invasive and native plant species remains largely uninvestigated. We conducted two microcosm experiments, one in which we amended the soil with mineral N and another in which we amended the soil with mineral P. In each experiment, we grew a pair of test plants consisting of Kummerowia striata (native to our research site) and Solidago canadensis (an invasive species). CMNs were established between the plants, and these were either left intact or severed. Intact CMNs increased growth and nutrient acquisition by S. canadensis while they decreased nutrient acquisition by K. striata in comparison with severed CMNs. 15N and P analyses indicated that compared to severed CMNs, intact CMNs preferentially transferred mineral nutrients to S. canadensis. CMNs produced by different species of AM fungi had slightly different effects on the interaction between these two plant species. These results highlight the role of CMNs in the understanding of interactions between the invasive species S. canadensis and its native neighbor.

Ecological mechanisms underlying the sustainability of the agricultural heritage rice-fish coculture system.

For centuries, traditional agricultural systems have contributed to food and livelihood security throughout the world. Recognizing the ecological legacy in the traditional agricultural systems may help us develop novel sustainable agriculture. We examine how rice-fish coculture (RF), which has been designated a "globally important agricultural heritage system," has been maintained for over 1,200 y in south China. A field survey demonstrated that although rice yield and rice-yield stability are similar in RF and rice monoculture (RM), RF requires 68% less pesticide and 24% less chemical fertilizer than RM. A field experiment confirmed this result. We documented that a mutually beneficial relationship between rice and fish develops in RF: Fish reduce rice pests and rice favors fish by moderating the water environment. This positive relationship between rice and fish reduces the need for pesticides in RF. Our results also indicate a complementary use of nitrogen (N) between rice and fish in RF, resulting in low N fertilizer application and low N release into the environment. These findings provide unique insights into how positive interactions and complementary use of resource between species generate emergent ecosystem properties and how modern agricultural systems might be improved by exploiting synergies between species.

Parasitism Shifts the Effects of Native Soil Microbes on the Growth of the Invasive Plant Alternanthera philoxeroides.

Soil microbes play an important role in plant invasion, and parasitic plants regulate the growth of invasive plants. However, the mechanisms by which parasitic plants regulate the effects of soil microbes on invasive plants have not been investigated. Here, we used the invasive plant Alternanthera philoxeroides and the holoparasitic plant Cuscuta grovonii to test whether and how C. grovonii parasitism shifts the effect of native soil microbes on the growth of A. philoxeroides. In a factorial setup, A. philoxeroides was grown in pots with the presence versus absence of parasitism and the presence versus absence of native soil microbes. The findings showed that native soil microbes increased the biomass and clonal growth of A. philoxeroides only in the absence of a parasite, whereas parasitism decreased the biomass and clonal growth of A. philoxeroides only in the presence of soil microbes. In addition, the presence of soil microbes increased the deleterious effects of the parasite on A. philoxeroides. These results indicate that parasitism can shift the effects of native soil microbes on the growth of the invasive plant A. philoxeroides. Our results enrich the understanding of the mechanisms underlying the success of plant invasion.

Resource availability and parasitism intensity influence the response of soybean to the parasitic plant Cuscuta australis.

Introduction: Parasitic plants can damage crop plants and consequently cause yield losses and thus threaten food security. Resource availability (e.g., phosphorus, water) has an important role in the response of crop plants to biotic attacks. However, how the growth of crop plants under parasitism are affected by environmental resource fluctuation is poorly understood. Methods: We conducted a pot experiment to test the effects of the intensity of Cuscuta australis parasitism and the availability of water and phosphorus (P) on soybean shoot and root biomass. Results and discussion: We found that low-intensity parasitism caused ~6% biomass reduction, while high-intensity parasitism caused ~26% biomass reduction in soybean. Under 5-15% water holding capacity (WHC), the deleterious effect of parasitism on soybean hosts was ~60% and ~115% higher than that under 45-55% WHC and 85-95% WHC, respectively. When the P supply was 0 µM, the deleterious effect of parasitism on soybean was 67% lower than that when the P supply was 20 µM. Besides, the biomass of C. australis was highest when both the water and the P availability were lowest. Cuscuta australis caused the highest damage to soybean hosts under 5 µM P supply, 5-15% WHC, and high-intensity parasitism. Additionally, C. australis biomass was significantly and negatively related to the deleterious effect of parasitism on soybean hosts and to the total biomass of soybean hosts under high-intensity parasitism, but not under low-intensity parasitism. Although high resource availability can promote soybean growth, the two resources have different impacts on the response of hosts to parasitism. Higher P availability decreased host tolerance to parasites, while higher water availability increased host tolerance. These results indicate that crop management, specifically water and phosphorus supply, can efficiently control C. australis in soybean. To our best knowledge, this appears to be the first study to test the interactive effect of different resources on the growth and response of host plants under parasitism.

Selectivity by host plants affects the distribution of arbuscular mycorrhizal fungi: evidence from ITS rDNA sequence metadata.

BACKGROUND: Arbuscular mycorrhizal fungi (AMF) can form obligate symbioses with the vast majority of land plants, and AMF distribution patterns have received increasing attention from researchers. At the local scale, the distribution of AMF is well documented. Studies at large scales, however, are limited because intensive sampling is difficult. Here, we used ITS rDNA sequence metadata obtained from public databases to study the distribution of AMF at continental and global scales. We also used these sequence metadata to investigate whether host plant is the main factor that affects the distribution of AMF at large scales. RESULTS: We defined 305 ITS virtual taxa (ITS-VTs) among all sequences of the Glomeromycota by using a comprehensive maximum likelihood phylogenetic analysis. Each host taxonomic order averaged about 53% specific ITS-VTs, and approximately 60% of the ITS-VTs were host specific. Those ITS-VTs with wide host range showed wide geographic distribution. Most ITS-VTs occurred in only one type of host functional group. The distributions of most ITS-VTs were limited across ecosystem, across continent, across biogeographical realm, and across climatic zone. Non-metric multidimensional scaling analysis (NMDS) showed that AMF community composition differed among functional groups of hosts, and among ecosystem, continent, biogeographical realm, and climatic zone. The Mantel test showed that AMF community composition was significantly correlated with plant community composition among ecosystem, among continent, among biogeographical realm, and among climatic zone. The structural equation modeling (SEM) showed that the effects of ecosystem, continent, biogeographical realm, and climatic zone were mainly indirect on AMF distribution, but plant had strongly direct effects on AMF. CONCLUSION: The distribution of AMF as indicated by ITS rDNA sequences showed a pattern of high endemism at large scales. This pattern indicates high specificity of AMF for host at different scales (plant taxonomic order and functional group) and high selectivity from host plants for AMF. The effects of ecosystemic, biogeographical, continental and climatic factors on AMF distribution might be mediated by host plants.

Effects of Parasitism on the Competitive Ability of Invasive and Native Species.

Parasitic plants can often seriously harm host plants and, thus, alter competitive dominance between hosts and neighbouring species. However, whether and how parasitic plants differently affect the competitive abilities of invasive and the native plants have not been tested. In this study, we used Cuscuta grovonii as the parasitic plants and three invasive plants and three native plants as host plants. Host plants grown alone or in competition with Coix lacryma-jobi were either parasitized with Cuscuta grovonii or not parasitized. Parasitism caused similar damage to invasive and native plants when grown with Cuscuta grovonii alone but caused less damage to invasive species than native species when grown in competition. Parasitism increased the competitive ability of invasive plants but did not affect the competitive ability of native plants. In the absence of parasitism, the competitive ability of host plants was significantly negatively correlated with the competitive ability of Coix lacryma-jobi, but under parasitism, there was no significant relationship of the competitive ability between host and competitor plants. Our results indicated that parasitic plants can increase the competitive tolerance of invasive plants, but have no effect on native plants. Thus, parasitism may play an important role in the process of plant invasion.

A parasite indirectly affects nutrient distribution by common mycorrhizal networks between host and neighboring plants.

Cascading effects are ubiquitous in nature and can modify ecological processes. Most plants have mutualistic associations with mycorrhizal fungi, and can be connected to neighboring plants through common mycorrhizal networks (CMNs). However, little is known about how the distribution of nutrients by CMNs to the interconnected plants is affected by higher trophic levels, such as parasitic plants. We hypothesized that parasitism would indirectly drive CMNs to allocate more nutrients to the nonparasitized neighboring plants rather than to the parasitized host plants, and that this would result in a negative-feedback effect on the growth of the parasitic plant. To test this, we conducted a container experiment, where each container housed two in-growth cores that isolated the root system of a single Trifolium pratense seedling. The formation of CMNs was either prevented or permitted using nylon fabric with a mesh width of 0.5 or 25 µm, respectively. In each container, either both T. pratense plants were not parasitized or only one was parasitized by the holoparasite Cuscuta australis. To quantify the nutrient distribution by CMNs to the host and neighboring plants, we used 15 N labeling. Growth and 15 N concentrations of C. australis and T. pratense were measured, as well the arbuscular mycorrhizal fungi-colonization rates of T. pratense. We found that parasitism by C. australis reduced the biomass of T. pratense. In the absence of the parasite, CMNs increased the 15 N concentration of both T. pratense plants, but did not affect their biomass. However, with the parasite, the difference between host and neighboring T. pratense plants in 15 N concentration and biomass were amplified by CMNs. Furthermore, CMNs decreased the negative effect of C. australis on growth of the host T. pratense plants. Finally, although CMNs did not influence the 15 N concentration of C. australis, they reduced its biomass. Our results indicate that when T. pratense was parasitized by C. australis, CMNs preferentially distributed more mineral nutrients to the nonparasitized neighboring T. pratense plant, and that this had a negative feedback on the growth of the parasite.

Irrigation and weed control alter soil microbiology and nutrient availability in North Carolina Sandhill peach orchards.

Orchard management practices such as weed control and irrigation are primarily aimed at maximizing fruit yields and economic profits. However, the impact of these practices on soil fertility and soil microbiology is often overlooked. We conducted a two-factor experimental manipulation of weed control by herbicide and trickle irrigation in a nutrient-poor peach (Prunus persica L. cv. Contender) orchard near Jackson Springs, North Carolina. After three and eight years of treatments, an array of soil fertility parameters were examined, including soil pH, soil N, P and cation nutrients, microbial biomass and respiration, N mineralization, and presence of arbuscular mycorrhizal fungi (AMF). Three general trends emerged: 1) irrigation significantly increased soil microbial biomass and activity, 2) infection rate of mycorrhizal fungi within roots were significantly higher under irrigation than non-irrigation treatments, but no significant difference in the AMF community composition was detected among treatments, 3) weed control through herbicides reduced soil organic matter, microbial biomass and activity, and mineral nutrients, but had no significant impacts on root mycorrhizal infection and AMF communities. Weed-control treatments directly decreased availability of soil nutrients in year 8, especially soil extractable inorganic N. Weed control also appears to have altered the soil nutrients via changes in soil microbes and altered net N mineralization via changes in soil microbial biomass and activity. These results indicate that long-term weed control using herbicides reduces soil fertility through reducing organic C inputs, nutrient retention and soil microbes. Together, these findings highlight the need for alternative practices such as winter legume cover cropping that maintain and/or enhance organic inputs to sustain the soil fertility.

An invasive plant promotes its arbuscular mycorrhizal symbioses and competitiveness through its secondary metabolites: indirect evidence from activated carbon.

Secondary metabolites released by invasive plants can increase their competitive ability by affecting native plants, herbivores, and pathogens at the invaded land. Whether these secondary metabolites affect the invasive plant itself, directly or indirectly through microorganisms, however, has not been well documented. Here we tested whether activated carbon (AC), a well-known absorbent for secondary metabolites, affect arbuscular mycorrhizal (AM) symbioses and competitive ability in an invasive plant. We conducted three experiments (experiments 1-3) with the invasive forb Solidago canadensis and the native Kummerowia striata. Experiment 1 determined whether AC altered soil properties, levels of the main secondary metabolites in the soil, plant growth, and AMF communities associated with S. canadensis and K. striata. Experiment 2 determined whether AC affected colonization of S. canadensis by five AMF, which were added to sterilized soil. Experiment 3 determined the competitive ability of S. canadensis in the presence and absence of AMF and AC. In experiment 1, AC greatly decreased the concentrations of the main secondary metabolites in soil, and the changes in concentrations were closely related with the changes of AMF in S. canadensis roots. In experiment 2, AC inhibited the AMF Glomus versiforme and G. geosporum but promoted G. mosseae and G. diaphanum in the soil and also in S. canadensis roots. In experiment 3, AC reduced S. canadensis competitive ability in the presence but not in the absence of AMF. Our results provided indirect evidence that the secondary metabolites (which can be absorbed by AC) of the invasive plant S. canadensis may promote S. canadensis competitiveness by enhancing its own AMF symbionts.