ABSTRACT
BACKGROUND: The omega-3 fatty acid (ω-3 fatty acid) such as eicosapentaenoic acid (EPA) is currently used in the clinic as a nutritional supplement in the treatment of poly- cystic ovarian syndrome (PCOS). The present study was designed to investigate the ef- fect of EPA on the expression levels of peroxisome proliferator-activated receptor gamma (PPARγ) and cytochrome P450 aromatase (encoded by the CYP-19) in primary cultured granulosa cells (GC) from patients undergoing in vitro fertilization (IVF), and also to compare these effects with those in GC of PCOS patients. MATERIALS AND METHODS: In this experimental study, human GC were isolated, pri- mary cultured in vitro, exposed to a range of concentrations of the EPA and in- vestigated with respect to gene expression levels of PPARγ and CYP-19 using real time-polymerase chain reaction (PCR). The participants (n=30) were the patients admitted to the IVF Center in February-March 2013 at Alzahra Hospital, Tabriz, Iran, who were divided into two groups as PCOS (n=15) and non-PCOS (n=15) women (controls). RESULTS: All doses of the EPA significantly induced PPARγ mRNA gene expression level as compared to the control recombinant follicle stimulating hormone (rFSH) alone condi- tion. High doses of EPA in the presence of rFSH produced a stimulatory effect on expres- sion level of PPARγ (2.15-fold, P=0.001) and a suppressive effect (0.56-fold, P=0.01) on the expression level of CYP-19, only in the PCOS GC. CONCLUSION: EPA and FSH signaling pathway affect differentially on the gene ex- pression levels of PPARγ and CYP-19 in PCOS GC. Altered FSH-induced PPARγ activity in PCOS GC may modulate the CYP-19 gene expression in response to EPA, and possibly modulates the subsequent steroidogenesis of these cells.
ABSTRACT
BACKGROUND: The omega-3 (ω-3) fatty acid eicosapentaenoic acid (EPA) is currently used in the clinic as a nutritional supplement to improve infertility, particularly in women with polycystic ovarian syndrome (PCOS). OBJECTIVE: The present study was designed to investigate the effect of EPA on insulin-like growth factor 1 (IGF-1) and cyclooxygenase 2 (COX-2) gene expression in primary cultured granulosa cells from patients undergoing in vitro fertilization (IVF), and also to compare this effect with those in granulosa cells of PCOS patients. MATERIALS AND METHODS: In this experimental study, human granulosa cells were isolated from follicular fluid of normal and PCOS women undergoing IVF by hyaluronidase digestions, followed by Percoll gradient centrifugation. Cells were cultured in vitro, exposed to a range of concentrations of the EPA (25-100 µM) for 24 hr, and investigated with respect to COX-2 and IGF-1 gene expression by real time-PCR. RESULTS: In both groups, all doses of the EPA significantly induced IGF-1 mRNA gene expression compared to the untreated control. High doses of EPA in the presence of recombinant (r) FSH produced a stimulatory effect on IGF-1 and a suppressive effect (p=0.01) on the COX-2 gene expression, which were more pronounced in granulosa cells from PCOS patients. CONCLUSION: EPA affect diversely the gene expression of IGF-1 and COX-2 in granulosa cells, which were more pronounced in PCOS compared to control. These findings represent the possible underlying molecular mechanisms for the positive impact of the ω-3 fatty acids on reproduction, especially in patients with PCOS.
ABSTRACT
Tooth crown discoloration may possess a heavy emotional burden and esthetic concern, especially when the anterior teeth are affected. Residue of sealers within the pulp chamber is a major contributor to the occurrence of tooth discoloration. Aim: The aim of this study was to evaluate the degree of crown discoloration when recently introduced sealer, Epiphany, is used. Methods: Forty human incisors were examined in vitro. Fifteen teeth were sealed with either AH26 or Epiphany sealer as experimental groups and root canals of the remaining 10 teeth with distilled water. Digital photographs of the buccal aspect of teeth were then examined in terms of brightness (value) and saturation (chroma) using Photoshop software at the beginning and consecutive 3, 6 and 9 months. Degree of brightness and saturation changes was defined as follows: less than 5% as slight, 10% to 15% as moderate and 15% to 20% as severe. Results: Fifteen, 14 and 10 teeth entered in Epiphany, AH26 and control groups, respectively. Significant brightness deterioration was noticed by time in both experimental groups [F (2, 8) =29.16, p<0.001], with no differences in Epiphany compared with AH26 (p=0.086). Saturation differed neither by time [F (2, 8) =0.129, p=0.881, nor by sealer type (p=0.136) during 9-month observation. At 9th month, crown segments (incisal, middle and cervical thirds) were not contrasted by the sealer type (p=0.982) or discoloration type (either brightness or saturation) (p=0.50). All changes in the experimental groups were significantly higher than the control group (p<0.001). Conclusions: Epiphany was equal to AH26 sealer in terms of relative long-term crown discoloration. One clinical correlation learned from the results of the present study is that Epiphany may safely be used interchangeably with the traditional AH26 sealer.