[To the question of the optimization of methods for detection of vinyl chloride and 1,2-dichloroethane, and their metabolites in biological fluids in workers involved in production of polyvinyl chloride].

There is considered the improvement of methodological approaches to the gas chromatographic methods- of the detection of vinyl chloride and 1,2-dichloroethane and their metabolites--chloroethanol and monochloroacetic acid in biological fluids. There were evaluated such metrological characteristics of methods, as repeatability, interlaboratoty precision, relevance and accuracy. The value of relative expanded uncertainty does not exceed 30%. There are reported optimal regimes of gas chromatographic analysis, conditions for sample preparation. The results of the contents ofthese chemical compounds and their metabolites in biological fluids from persons working in contact with chlorinated hydrocarbons are presented These techniques can be used for the detection ofthe fact of exposure to toxic substances, assessment of the level of exposure and biomonitoring.

Covalent binding of reactive intermediates to hemoglobin in the mouse as an approach to studying the metabolic pathways of 1,2-dichloroethane.

CBA mice were given 14C-labelled 1,2-dichloroethane by intraperitoneal injection. Metabolic pathways leading to the formation of electrophilic intermediates were studied through the determination, by ion-exchange chromatography, of the products of reaction of this labelled substrate with nucleophilic sites in hemoglobin and DNA. The products found in hemoglobin and the pattern of alkylation suggest that chloroacetaldehyde and S-(2-chloroethyl)glutathione are important reactive metabolites in vivo. The alkyl purines, 7-(2-oxoethyl)guanine and 7-[S-(2-cysteine)-ethyl]guanine, were found in DNA hydrolysates, as well as in the urine.