RESUMEN
The study of isolated cardiomyocytes after alkaline dissociation allowed to reveal an individual variability of the absolute number and average volume of cells, as well as characteristics of these indices in the left and right ventricle myocardia of the dead adult patients' hearts. On the basis of the analysis of these indices and of other parameters of isolated cardiomyocytes, a possibility of hyperplasia of nuclei and of muscle cells themselves during their hypertrophy is discussed.
Asunto(s)
Ventrículos Cardíacos/citología , Adulto , Recuento de Células , Núcleo Celular/ultraestructura , Ventrículos Cardíacos/patología , Ventrículos Cardíacos/ultraestructura , Humanos , HiperplasiaRESUMEN
Alkaline dissociation of cardiomyocytes was used for investigation of concentric and eccentric myocardial hypertrophy in 8 deceased patients with carions ventricular myocardial mass. For both ventricles and forms of hypertrophy the length, diameter and volume of the cells were found increased, the increase being proportional for the left ventricle (except the volume) and gross for linear enlargement of the right ventricle in eccentric hypertrophy. Absolute number of cardiomyocytes reached maximum in concentric left ventricular hypertrophy showing the same values with normal-mass ventricles in eccentric hypertrophy.
Asunto(s)
Cardiomegalia/patología , Miocardio/patología , Adulto , Anciano , Cardiomegalia/clasificación , Recuento de Células , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Data are given on the laboratory spontaneous occurrence of trypanosomiasis in young rats. It was demonstrated that the louse Polyplax spinulosa is the main mechanical vector of Trypanosoma lewisi. Unlike the case of the real host, the flea, the trypanosomes do not undergo the whole developmental cycle in the louse.
Asunto(s)
Phthiraptera/parasitología , Tripanosomiasis/transmisión , Animales , Ratas , Factores de Tiempo , Tripanosomiasis/parasitologíaAsunto(s)
Traumatismos por Electricidad/patología , Rayos Láser/efectos adversos , Piel/lesiones , Animales , Cadáver , Humanos , PorcinosAsunto(s)
Rayos Láser/efectos adversos , Textiles/efectos de la radiación , Cadáver , Medicina Legal , HumanosRESUMEN
Using a modified method for alkalic dissociation, isolated ventricular cardiomyocytes were obtained from eight patients who had died of different diseases. The absolute number of cardiomyocytes, counted in the Fuchs-Rosenthal chamber, ranged between 1.10 x 10(9) and 3.25 x 10(9) in the left ventricle, and between 0.58 x 10(9) and 1.42 x 10(9) in the right ventricle depending on myocardial mass. Mean volume of cells ranged between 75 x 10(3) and 344 x 10(3) microns3, and the content of polynuclear, predominantly binucleated, cardiomyocytes amounted to 14 to 49%. An increase in ventricular myocardium mass was associated with an increase in cardiomyocyte volume. A negative correlation was demonstrated between the absolute number of cardiomyocytes on the one hand, and their mean volume and proportion of polynuclear cells on the other.