Green synthesis of biomass-derived carbon quantum dots for photocatalytic degradation of methylene blue.

Water pollution, significantly influenced by the discharge of synthetic dyes from industries, such as textiles, poses a persistent global threat to human health. Among these dyes, methylene blue, particularly prevalent in the textile sector, exacerbates this issue. This study introduces an innovative approach to mitigate water pollution through the synthesis of nanomaterials using biomass-derived carbon quantum dots (CQDs) from grape pomace and watermelon peel. Utilizing the hydrothermal method at temperatures between 80 and 160 °C over periods ranging from 1 to 24 h, CQDs were successfully synthesized. A comprehensive characterization of the CQDs was performed using UV-visible spectroscopy, Fourier-transform infrared spectroscopy, dynamic light scattering, Raman spectroscopy, and luminescence spectroscopy, confirming their high quality. The photocatalytic activity of the CQDs in degrading methylene blue was evaluated under both sunlight and incandescent light irradiation, with measurements taken at 20 min intervals over a 2 h period. The CQDs, with sizes ranging from 1-10 nm, demonstrated notable optical properties, including upconversion and down-conversion luminescence. The results revealed effective photocatalytic degradation of methylene blue under sunlight, highlighting the potential for scalable production of these cost-effective catalytic nanomaterials for synthetic dye degradation.

Monolayer (2D) or spheroids (3D) cell cultures for nanotoxicological studies? Comparison of cytotoxicity and cell internalization of nanoparticles.

Two-dimensional (2D) cell culture monolayers are commonly used for toxicological assessments of nanomaterials. Despite their facile handling, they exhibit several constraints due to their structural and complexity differences with three-dimensional (3D) in vitro cell models, such as spheroids. Here, we conducted a comparative nanotoxicological study of fibroblasts (L929) and melanoma (B16-F10) cells, grown in 2D and 3D arrangements. The cytotoxicity, reactive oxygen species (ROS) production, genotoxicity, cell morphology complexity, and uptake of silver nanoparticles (AgNPs) and folic acid-functionalized upconversion nanoparticles (FA-UCNPs) were compared in the two culture arrangements. AgNPs cytotoxicity was higher in spheroids than in monolayer cultures. Furthermore, apoptotic cell percentages and ROS production were higher in 3D than in 2D cell cultures. More importantly, 2D cultures required twice the concentration of AgNPs than the 3D cell models to reach a considerable DNA damage index (c.a. 200). Therefore, spheroids are more sensitive to the genotoxic effects of AgNPs. FA-UCNPs exerted negligible cell toxicity in 2D and 3D cell models. Moreover, AgNPs induced disaggregation and downsizing of spheroids in a facile and concentration-dependent manner. Internalization of FA-UCNPs in spheroids was 20% higher than in the 2D cell arrangements. Collectively, our findings, demonstrated that spheroids are a more sensitive model than monolayers for the assessment of nanoparticle biocompatibility and internalization.

Nanotoxicological study of downconversion Y2 O3 :Eu3+ luminescent nanoparticles functionalized with folic acid for cancer cells bioimaging.

Luminescent lanthanide downconversion nanoparticles (DCNPs) provide a combination of high luminescence intensity, sharp emission peaks with narrow bandwidth and a large Stokes' shift, leading to high-performance biomedical applications mainly for imaging. The purpose of this study is to present a nanotoxicological study of DCNPs Y2 O3 codoped with Eu3+ and functionalized with folic acid (FA). These assessments include cytotoxicity, genotoxicity, hemocompatibility, and in vitro inflammatory studies. We demonstrated by flow cytometry and confocal microscope the internalization of FA-DCNPs in breast cancer and melanoma cells. They were synthesized by sol-gel method and coated with a thin silica shell to make them biocompatible; also they were functionalized with amino groups and FA ligands that bind to the folate receptors (FR) located on the surface of the cancer cells studied. This functionalization enables the DCNPs to be internalized into the cancer cells via endocytosis by the conjugation FA-FR. The DCNPs were characterized with transmission electron microscope, Fourier transform infrared spectroscopy and photoluminescence. The nanotoxicological assessments demonstrated that both nanoparticles (bare and functionalized) are no cytotoxic and no genotoxic at the tested concentrations (0.01-20 µg/mL) in three cell lines (breast, skin cancer, and osteoblasts). Also they are hemocompatible and do not exert nitric oxide production in vitro by macrophages. The FA-DCNPs were clearly localized into the cell cytoplasm with bright red luminescence. Thus, herein we present a complete nanotoxicological study of FA-DCNPs Y2 O3 codoped with Eu3+ and we conclude that these nanoparticles are biocompatible and can be further used for cancer cells bioimaging.