PHBV-TiO2 mats prepared by electrospinning technique: Physico-chemical properties and cytocompatibility.

One of the most important challenges in tissue engineering research is the development of biomimetic materials. In this present study, we have investigated the effect of the titanium dioxide (TiO2 ) nanoparticles on the properties of electrospun mats of poly (hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), to be used as scaffold. The morphology of electrospun fibers was observed by scanning electron microscopy (SEM). Both pure PHBV and nanocomposites fibers were smooth and uniform. However, there was an increase in fiber diameter with the increase of TiO2 concentration. Thermal properties of PHBV and nanocomposite mats were characterized by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). DSC analysis showed that the crystallization temperature for PHBV shifts to higher temperature in the presence of the nanoparticles, indicating that TiO2 nanoparticles change the process of crystallization of PHBV due to heterogeneous nucleation effect. TGA showed that in the presence of the nanoparticles, the curves are shifted to lower temperatures indicating a decreasing in thermal stability of nanocomposites compared to pure PHBV. To produce scaffolds for tissue engineering, it is important to evaluate the biocompatibility of the material. Cytotoxicity assay showed that TiO2 nanoparticles were not cytotoxic for cells at the concentration used to synthesize the mats. The proliferation of cells on the mats was evaluated by the MTT assay. Results showed that the nanocomposite samples increased cell proliferation compared to the pure PHBV. These results indicate that continuous electrospun fibrous scaffolds may be a good substrate for tissue regeneration.

Energy transfer mechanisms in pH-tuned aggregates of curcumin.

Curcumin ((1,7-bis-(4-hidroxi-3-metoxifenil)-1,6-heptadieno-3,5-diona)), is a natural yellow-orange polyphenol dye, obtained from Curcuma longa rhizomes, with vast potential in the biotechnology field, such as anti - inflammatory and cancer preventive properties, and a very environmental-sensible structure, mostly resulting in aggregate formations, with low solubility in aqueous solvents and remarkable pH dependence. Hence, it is essential to comprehend its aggregation mechanisms, optical properties and conformational equilibrium to develop curcumin-based drugs and biomaterials for several applications. With the purpose of understand such concepts and its properties, the results presented in this work, obtained through spectroscopic measurements and the Hill's modeling, show a conformational equilibrium, driven by pH, between the enol-enolate and aggregated forms in curcumin, and the respective energy transfer processes between these species. Additionally, the photophysical mechanisms for the energy transfer between aggregated forms, observed with the ellipsometry measurements, showed an "all-or-nothing" character, pointing to a very complex conformation for curcumin aggregates.

Lead detection using micro/nanocrystalline boron-doped diamond by square-wave anodic stripping voltammetry.

Monitoring heavy metal ion levels in water is essential for human health and safety. Electroanalytical techniques have presented important features to detect toxic trace heavy metals in the environment due to their high sensitivity associated with their easy operational procedures. Square-wave voltammetry is a powerful electrochemical technique that may be applied to both electrokinetic and analytical measurements, and the analysis of the characteristic parameters of this technique also enables the mechanism and kinetic evaluation of the electrochemical process under study. In this work, we present a complete optimized study on the heavy metal detection using diamond electrodes. It was analyzed the influence of the morphology characteristics as well as the doping level on micro/nanocrystalline boron-doped diamond films by means of square-wave anodic stripping voltammetry (SWASV) technique. The SWASV parameters were optimized for all films, considering that their kinetic response is dependent on the morphology and/or doping level. The films presented reversible results for the Lead [Pb (II)] system studied. The Pb (II) analysis was performed in ammonium acetate buffer at pH 4.5, varying the lead concentration in the range from 1 to 10 µg L(-1). The analytical responses were obtained for the four electrodes. However, the best low limit detection and reproducibility was found for boron doped nanocrystalline diamond electrodes (BDND) doped with 2000 mg L(-1) in B/C ratio.