Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 6 de 6
Filtrar
1.
BMC Vet Res ; 16(1): 397, 2020 Oct 20.
Artículo en Inglés | MEDLINE | ID: mdl-33081763

RESUMEN

BACKGROUND: Wounds cause structural and functional discontinuity of an organ. Wound healing, therefore, seeks to re-establish the normal morphology and functionality through intertwined stages of hemostasis, inflammation, proliferation, and tissue remodelling. Ivermectin, a macrolide, has been used as an endectoparasiticide in human and veterinary medicine practice for decades. Here, we show that ivermectin exhibits wounding healing activity by mechanisms independent of its well-known antiparasitic activity. This study aimed to evaluate the wound healing property of ivermectin cream using histochemistry and enzyme-linked immunosorbent assay techniques. RESULTS: Non-irritant dose of ivermectin cream (0.03-1%) decreased wound macroscopic indices such as exudation, edge edema, hyperemia, and granulation tissue deposition by day 9 compared to day 13 for the vehicle-treated group. This corresponded with a statistically significant wound contraction rate, hydroxyproline deposition, and a decreased time to heal rate. The levels of growth factors TGF-ß1 and VEGF were significantly elevated on day 7 but decreased on day 21. This corresponded with changes in cytokines (IL-1α, IL-4, IL-10, and TNF-α) and eicosanoids (LTB4, PGE2, and PGD2) levels on days 7 and 21.. Interestingly, low doses of ivermectin cream (0.03-0.1%) induced wound healing with minimal scarring compared to higher doses of the cream and the positive control, Silver Sulfadiazine. CONCLUSION: Ivermectin promotes wound healing partly through modulation of the inflammatory process and the levels of Transforming Growth Factor-Beta 1 and Vascular Endothelial Growth Factor. Low doses of ivermectin cream have the potential to be used in treating wounds with minimal scar tissue formation.


Asunto(s)
Ivermectina/farmacología , Piel/efectos de los fármacos , Cicatrización de Heridas/efectos de los fármacos , Animales , Ivermectina/toxicidad , Masculino , Ratas Sprague-Dawley , Sulfadiazina de Plata/farmacología , Factor de Crecimiento Transformador beta1/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo
2.
J Immunoassay Immunochem ; 41(2): 152-157, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32028862

RESUMEN

Q fever, caused by Coxiella burnetii, is an important zoonosis worldwide. Q fever is documented in many parts of the world; however, information on the disease in Ghana is scanty. This study was therefore conducted to provide evidence of exposure of sheep and goats slaughtered at the Kumasi Abattoir to Coxiella burnetii. A total of 350 serum samples collected from 175 sheep and 175 goats were analyzed for the presence of C. burnetii antibodies using a commercial ELISA kit (ID Vet). Results of the study established a seroprevalence of 28.57% in goats, 16.57% in sheep and an overall seroprevalence of 22.29% in sheep and goats; 20.57% for male sheep, 23.86% for female sheep, 26.44% for male goats and 30.68% for female goats. Results showed that goats are more at risk to the infection than sheep however sex is not a risk factor. This study confirms the existence of Q fever in sheep and goats in Ghana hence, the disease should be considered as a public health risk to workers at the abattoir and other stakeholders in the sheep and goat production chain.


Asunto(s)
Infecciones Bacterianas/inmunología , Coxiella burnetii/inmunología , Enfermedades de las Cabras/inmunología , Enfermedades de las Ovejas/inmunología , Animales , Infecciones Bacterianas/sangre , Infecciones Bacterianas/microbiología , Estudios Transversales , Ensayo de Inmunoadsorción Enzimática , Femenino , Ghana , Enfermedades de las Cabras/sangre , Enfermedades de las Cabras/microbiología , Cabras , Masculino , Factores de Riesgo , Ovinos , Enfermedades de las Ovejas/sangre , Enfermedades de las Ovejas/microbiología
3.
J Immunoassay Immunochem ; 40(3): 300-313, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30894070

RESUMEN

There is a decline in sheep production and contribution to the economy. This study described the histological pattern, immunohistochemically demonstrate bacterial and viral agents of pneumonia in sheep slaughtered in metropolitan abattoirs in Nigeria and Ghana. A total of 805 (600 in Ghana and 205 in Nigeria) sheep lungs were examined for pneumonic lesions. Sections were fixed in 10% formalin for histological and immunohistochemical analysis. Pneumonia of 8.7% was in Ghana and 13.3% in Nigeria. The histological findings included bronchopneumonia (16/70), broncho-interstitial pneumonia (9/70), interstitial pneumonia (9/70) and proliferative pneumonia (7/70). Ten (14.3%) cases were positive for PPRV, PI3V, and RSV antigens while five (7.2%) were positive for multiple viral antigens; including two (PI3V and PPRV), two (RSV and PPRV), and one (PI3V and RSV). RSV was in interstitial pneumonia, PPRV and PI3V were in interstitial and broncho-interstitial pneumonia. PPRV and PI3V antigens 3 (4.4%) were in proliferative alveolitis. Mannheimia haemolytica (2) and Pasteurella multocida (2) infection were in combination with PPRV, RSV, and PI3V. Three sheep lungs (4.4%) were negative for viruses and bacteria. This study revealed ovine pneumonia is still a problem in West Africa, and multivalent vaccine incorporating some of these agents will be useful for the control.


Asunto(s)
Neumonía/veterinaria , Enfermedades de las Ovejas/diagnóstico , Enfermedades de las Ovejas/patología , Animales , Ghana , Inmunohistoquímica , Nigeria , Neumonía/diagnóstico , Neumonía/patología , Ovinos
4.
J Immunoassay Immunochem ; 40(5): 495-501, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31317821

RESUMEN

Toxoplasmosis, caused by T. gondii, is an important zoonosis worldwide. In Ghana, information on the disease in humans abounds but scanty in animals. This study was therefore conducted to estimate the seroprevalence of T. gondii infection sheep and goats sampled from the Kumasi Abattoir in Ashanti Region, Ghana. A total of 347 serum samples collected from 170 sheep and 177 goats were analyzed for the presence of T. gondii antibodies using a commercial ELISA kit. Results of this study estimated the seroprevalence of 23.7% in goats an, 35.9% in sheep. In sheep, 24 (35.82%) out of a total of 67 male samples were positive and 37 (35.92%) out of a total of 104 female samples were positive while in goats, 6 (8.2%) bucks out of a total of 73 were positive while 36 (34.6%) does out of a total of 104 were positive. There was a significant difference in the rate of seropositivity of female goats (p-value 0.01). This study confirms the existence of T. gondii infection in small ruminants in Ghana and it showed that sheep and dogs are more at risk to T. gondii infection hence meat from such animals could be a potential risk to public health if consumed raw or undercooked.


Asunto(s)
Anticuerpos Antiprotozoarios/inmunología , Cabras/inmunología , Cabras/parasitología , Ovinos/inmunología , Ovinos/parasitología , Toxoplasma/inmunología , Animales , Anticuerpos Antiprotozoarios/sangre , Estudios Transversales , Femenino , Ghana , Humanos , Masculino , Estudios Seroepidemiológicos
5.
Transbound Emerg Dis ; 69(4): e71-e81, 2022 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-34331389

RESUMEN

Dog-mediated rabies is responsible for approximately 60,000 human deaths annually worldwide. Although dog slaughter for human consumption and its potential risk for rabies transmission has been reported, mainly in some parts of Western Africa and South-East Asia, more information on this and factors that influence dog meat consumption is required for a better understanding from places like Ghana where the practice is common. We tested 144 brain tissues from apparently healthy dogs slaughtered for human consumption for the presence of rabies viruses using a Lyssavirus-specific real-Time RT-PCR. Positive samples were confirmed by virus genome sequencing. We also administered questionnaires to 541 dog owners from three regions in Ghana and evaluated factors that could influence dog meat consumption. We interacted with butchers and observed slaughtering and meat preparation procedures. Three out of 144 (2.1%) brain tissues from apparently healthy dogs tested positive for rabies virus RNA. Two of the viruses with complete genomes were distinct from one another, but both belonged to the Africa 2 lineage. The third virus with a partial genome fragment had high sequence identity to the other two and also belonged to the Africa 2 lineage. Almost half of the study participants practiced dog consumption [49% (265/541)]. Males were almost twice (cOR = 1.72, 95% CI (1.17-2.52), p-value = .006) as likely to consume dog meat compared to females. Likewise, the Frafra tribe from northern Ghana [cOR = 825.1, 95% CI (185.3-3672.9), p-value < .0001] and those with non-specific tribes [cOR = 47.05, 95% CI (10.18-217.41), p-value < .0001] presented with higher odds of dog consumption compared to Ewes. The butchers used bare hands in meat preparation. This study demonstrates the presence of rabies virus RNA in apparently healthy dogs slaughtered for human consumption in Ghana and suggests a potential risk for rabies transmission. Veterinary departments and local assemblies are recommended to monitor and regulate this practice.


Asunto(s)
Enfermedades de los Perros , Virus de la Rabia , Rabia , Enfermedades de las Ovejas , Animales , Enfermedades de los Perros/epidemiología , Perros , Femenino , Ghana/epidemiología , Humanos , Masculino , Carne , ARN , Rabia/epidemiología , Rabia/veterinaria , Virus de la Rabia/genética , Ovinos
6.
Zoonoses Public Health ; 66(2): 232-247, 2019 03.
Artículo en Inglés | MEDLINE | ID: mdl-30680936

RESUMEN

Influenza viruses are frequently transmitted between pigs and their handlers, and among pig handlers. However, reports on socio-environmental variables as potential risk factors associated with transmission of influenza in West African swine production facilities are very scarce. Syndromic survey for influenza was therefore conducted in Ibadan, Nigeria, and Kumasi, Ghana, in order to identify and elucidate selected socio-environmental variables that may contribute to the occurrence and distribution of influenza-like illness (ILI) among swine industry workers. In addition, molecular analyses were conducted to elucidate the nature of influenza viruses circulating at the human-swine interface in these cities and better understand the dynamics of their transmission. Influenza viruses were detected by type-specific and subtype-specific RT-PCR. Sequencing and phylogenetic analyses were carried out. Socio-environmental variables were tested by both univariable and multivariable regression methods for significance at p < 0.05. Three risk factors for ILI were identified in each city. These included "frequency of visit of pig handler to pig pen or lairage" (Ibadan: risk ratio [RR] = 1.54, 95% confidence interval [CI] = 1.36-1.79, p = 0.02; Kumasi: RR = 1.28, 95% CI = 1.11-1.71, p = 0.01) and "pig handler's awareness about biosecurity measures" (Ibadan: RR = 7.09, 95% CI = 2.36-21.32, p < 0.001; Kumasi: RR = 4.84, 95% CI = 1.98-11.80, p < 0.001). Influenza A(H1N1)pdm09 viruses, with M genes closely related to those which circulated among pigs in the two cities during the same period, were detected among Nigerian and Ghanaian pig industry workers. These findings suggest the possibility of bidirectional transmission of influenza at the human-swine interface in these cities and underscore the need for more extensive molecular studies. Risk factors identified may assist in the control of human-to-human and human-to-swine transmission of influenza in the West African swine industry.


Asunto(s)
Gripe Humana/transmisión , Infecciones por Orthomyxoviridae/veterinaria , Orthomyxoviridae/genética , Enfermedades de los Porcinos/transmisión , Zoonosis/transmisión , Animales , Anticuerpos Antivirales/sangre , Ciudades/epidemiología , Femenino , Ghana/epidemiología , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Subtipo H1N1 del Virus de la Influenza A/genética , Subtipo H3N2 del Virus de la Influenza A/genética , Gripe Humana/epidemiología , Gripe Humana/virología , Masculino , Nigeria/epidemiología , Infecciones por Orthomyxoviridae/epidemiología , Infecciones por Orthomyxoviridae/transmisión , Filogenia , Reacción en Cadena de la Polimerasa , Factores de Riesgo , Porcinos , Enfermedades de los Porcinos/virología , Zoonosis/epidemiología , Zoonosis/virología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA