RESUMEN
DNA barcoding of 172 anecic Octodrilus specimens collected in NE Italy and bordering Croatia has been carried out. The Bayesian phylogenetic tree showed high support for almost all currently recognized species, however, some unexpected results also appeared. The clade representing Oc. pseudocomplanatus contains a highly advanced subclade, which morphologically resembles Oc. slovenicus. The highly supported Oc. tergestinus clade consists of four unresolved divergent lineages of which the first corresponds to Oc. istrianus and the second resembles Oc. mimus morphologically; the third and fourth clades show typical tergestinus characters. The widely distributed Oc. complanatus consists of three highly divergent subclades which are sister to a new species, Oc. zicsiniello sp. nov., hereunder described.
Asunto(s)
Código de Barras del ADN Taxonómico , Oligoquetos , Filogenia , Animales , Teorema de Bayes , Croacia , Granjas , Bosques , ItaliaRESUMEN
A new Italian earthworm morphologically close to the similarly large and anecic Eophila tellinii (Rosa, 1888) is described. Distribution of Eophila crodabepis sp. nov. extends over 750 km2 from East to West on the Asiago Plateau and Vittorio Veneto Hills, from North to South on mounts Belluno Prealps (Praderadego and Cesen), Asiago, Grappa and onto the Montello foothills. This range abuts that of Eophila tellinii in northern Friuli Venezia Giulia region. Known localities of both E. tellinii and E. crodabepis sp. nov. are mapped. mtDNA barcoding definitively separates the new western species from classical Eophila tellinii (Rosa, 1888).
Asunto(s)
Código de Barras del ADN Taxonómico/métodos , ADN Mitocondrial/genética , Oligoquetos/genética , Análisis de Secuencia de ADN/métodos , Animales , ADN Mitocondrial/química , Geografía , Italia , Oligoquetos/anatomía & histología , Oligoquetos/clasificación , Filogenia , Especificidad de la EspecieRESUMEN
[This corrects the article DOI: 10.1371/journal.pone.0151799.].