A case of polyostotic osteosarcoma with kidney metastases in a dog: histopathology and microcomputed tomographic analysis.

A 7-year- old sexually intact female Leonberg dog was evaluated for chronic lameness of the right forelimb. The bitch showed mild hyperthermia (39.3°C), a decrease in its activity, a capricious appetite, a high weight loss (4 kg in 15 days) and a right foreleg lameness. A careful clinical examination revealed a deformation of the right proximal humerus and right tibia. Radiographic examination of the right tibia, right humerus showed osteolysis of both cortical and trabecular bone with a periosteal bone proliferation in the vicinal soft tissues. The owner having refused a bone biopsy, a treatment with NSAIDs and antibiotics was prescribed. After a marked improvement during the first two weeks, an increase in lameness and activity was observed. At that time, the owner accepted the bone biopsy. Histopathologic examination evidenced an osteosarcoma but the amount of available tissue was limited. Due to the poor prognosis, he declined treatment and decided to euthanize the dog. An osteosarcoma with a large chondroid component was observed at autopsy together with ossifying kidney metastases. Histological findings revealed a grade III osteosarcoma. Conventional and undecalcified histology and X-ray microcomputed tomography findings evidenced a large and partially mineralized osteoid part with a sunburst extension in the soft tissues. This is the first time that microCT and undecalcified analyses of an osteosarcoma are presented. The osteolytic and metaplastic bone foci were easily demonstrated by this method.

Comparative anatomical study of sound production and reception systems in the common dolphin (Delphinus delphis) and the harbour porpoise (Phocoena phocoena) heads.

Magnetic resonance imaging (MRI) and computed tomography (CT) scans were used to analyse, respectively, the soft tissues and the bones of the heads of four common dolphins and three harbour porpoises. This imaging study was completed by an examination of anatomical sections performed on two odontocete heads (a subadult common dolphin and a subadult harbour porpoise). The three complementary approaches allowed to illustrate anatomical differences in the echolocation systems of the common dolphin and the harbour porpoise. We captured images confirming strong differences of symmetry of the melon and of its connexions to the MLDB (Monkeys Lips/Dorsal Bursae) between the common dolphin and the harbour porpoise. The melon of the common dolphin is asymmetrically directly connected to the right bursae cantantes at its right side, whereas the melon of the harbour porpoise is symmetrical, and separated from the two bursae cantantes by a set of connective tissues. Another striking difference comes from the bursae cantantes themselves, less deeply located in the head of the common dolphin than in the harbour porpoise.

Magnetic Resonance Imaging Study of Adipose Tissues in the Head of a Common Dolphin (Delphinus delphis): Structure Identification and Influence of a Freezing-Thawing Cycle.

Magnetic resonance imaging (MRI) was used to scan the head of a common dolphin (Delphinus delphis) in order to visualize the different adipose tissues involved in echolocation functioning and to precisely delineate their anatomical topology. MRI scans were performed on the head taken from a freshly stranded carcass and repeated after a 2-week freezing time followed by thawing. The main fatty organs of the head, that is the melon, the mandibula bulba, the bursae cantantes, and their different connections with surrounding tissues were identified and labelled. The nasal sacs, other organs of echolocation, were also identified and labelled thanks to different MRI acquisitions. The shape, the location, the type of MRI signal of each organ and of their different connections were successfully analysed on all images, and then, the images of the head fresh or after thawing were compared. No impacts of the freezing/thawing cycle on the fatty tissues of the head were identified. Different parts were distinguished in the melon on the basis of the MRI signal emitted, corresponding most likely to the internal and external melon already identified by other analytical approaches, and linked to differences in lipid composition. MRI is shown here to be a useful tool to study the functional anatomy of the organs responsible for echolocation in odontocetes, with a particularly high level of precision.

Venous Valvular Distribution in the Thoracic and Pelvic Limbs of the Horse.

Dysfunction of venous valves can lead to hemodynamic disorders causing venous stasis, which would favour the occurrence of equine laminitis. However, very few studies have investigated venous valves in the horse digit. The purpose of this study was to compare valvular density between thoracic and pelvic limbs and to study the relationship between valvular density of veins and their location, diameter and wall thickness. After dissection, valvular density was calculated based on the number of valves counted in the principal veins of 7 thoracic and 7 pelvic limbs from 7 horses. Our results showed that the valvular density was higher in thoracic limbs, which probably reflects the adaptation to the consequences of hydrostatic pressure. The superficial veins have a higher valvular density that would prevent the varicose risk in the horse. The lower valvular density in the thick veins can be explained by the high density of the smooth muscular cells contained, which would cause an important vasoconstriction via the sympathetic nervous system. The veins with a large diameter also have a lower valvular density; these veins are not exposed to important changes in hydrostatic pressure. Other valvular characteristics may also be involved in the vascular disorders that may be related to the pathophysiology of laminitis.

No need to open the jar: a comparative study of Magnetic Resonance Imaging results on fresh and alcohol preserved common carps (Cyprinus carpio (L. 1758), Cyprinidae, Teleostei).

Magnetic Resonance Imaging (MRI) examinations have been conducted both on fresh and alcohol-preserved common carps (Cyprinus carpio). The images have been compared to those of a sagittally-cut frozen animal of the same species. This work shows that the images obtained are globally similar, and that the MRI technique can be applied to investigate the gross anatomy of alcohol-preserved specimens without destroying them. Unfortunately, this kind of study does not provide precise enough anatomical data for small specimens (less than 10 cm in total length) with a 1 Tesla magnetic field. Nevertheless, leaving the specimen in the jar during MRI examinations does not affect the quality of the final images.

Anatomical and descriptive study of the radial extensor muscle (M. extensor carpi radialis).

The M. extensores carpi radiales have been studied in detail in humans. The aim of this study was to carry out a comparative anatomical study of these muscles using four species--rabbit, cat, dog and sheep--and to propose a 'systematization' in a few standard models according to the morphological variations seen. In these species, there is marked morphological evolution, with two muscles in humans, one in sheep and a more or less distinct division of the muscle in the rabbit, cat and dog. Examination of the vascularization and nerve supply enables us to determine degrees of division in species with similar muscle morphology. Thus we were able to distinguish three morphological types which allow us to infer the morphological evolution of the M. extensores carpi radiales and to estimate the point at which one muscle became two. However, there is a strong chance that some process of convergence may have occurred, and in pentadactyl species many elements represent the plesiomorphis and are therefore of little use in constructing a classification on the basis of evolution.