RESUMEN
An 8-year-old dog presented with several dermal excoriations. Lesion cytology revealed pyogranulomatous inflammation with branching, septate hyphae. A mold identified as Mycoleptodiscus indicus by morphology and sequencing was cultured from fine-needle aspirates. This is the first report of a Mycoleptodiscus species as an etiologic agent in a dog.
Asunto(s)
Ascomicetos/aislamiento & purificación , Enfermedades de los Perros/diagnóstico , Enfermedades de los Perros/microbiología , Micosis/veterinaria , Infecciones de los Tejidos Blandos/veterinaria , Animales , ADN de Hongos/química , ADN de Hongos/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Perros , Histocitoquímica , Huésped Inmunocomprometido , Masculino , Microscopía , Datos de Secuencia Molecular , Micosis/diagnóstico , Micosis/microbiología , Análisis de Secuencia de ADN , Piel/patología , Infecciones de los Tejidos Blandos/diagnóstico , Infecciones de los Tejidos Blandos/microbiologíaRESUMEN
A 4-year-old female spayed Bichon Frise dog that had been receiving cyclosporine A per os 3 times per week for 2 months to control allergic dermatitis developed lethargy, anorexia, fever, and multiple firm subcutaneous masses. Pyogranulomatous inflammation with branching nonseptate filamentous organisms approximately 2 µm in diameter, presumptively fungal organisms, was diagnosed by cytologic evaluation of fine-needle aspirates from several masses. A partially acid-fast actinomycete was cultured from 2 of the masses. The organism was identified as Nocardia abscessus (formerly Nocardia asteroides type 1) based on 16S ribosomal DNA sequencing of samples extracted from cultures and unstained cytologic smears. Immunosuppression caused by long-term administration of cyclosporine A likely predisposed the dog to disseminated infection. To our knowledge, this is the first report of N. abscessus infection in a dog. This case demonstrates that N. abscessus may be mistaken for a fungal organism based on its cytologic appearance and underscores the importance of using molecular techniques for the diagnosis of suspected fungal diseases.