RESUMEN
Extracts from encephalomyocarditis (EMS) virus infected Krebs II ascites carcinoma cells pulse-labeled during the active virus-specific synthesis and then chased were fractionated in a sucrose concentration gradient. It has shown that some radioactivity was detectable in the polysome region as well as in the regions of ribosome monomers and ribosomal subunits. An analysis of the radioactive material in a CsCl density gradient and by polyacrylamide gel electrophoresis has shown that components of the protein-synthesizing system in infected cells are bound to some proteins, the electrophoretic mobility of which corresponds to that of polypeptides found in the infected cells, namely, polypeptides G 16 (18 kdalton) and 22 (22 kdalton). The ribosomes from normal cells were also found to be associated with three labeled polypeptides, their molecular weight (89-90, 43-48 and 39-40 kdalton) being different from those of the polypeptides bound to the ribosomes from the infected cells. Thus, the presence of polypeptides G and 22 is specific for ribosomes isolated from the infected cells. The possible significance of this binding is discussed.
Asunto(s)
Carcinoma Krebs 2/metabolismo , Virus de la Encefalomiocarditis/metabolismo , Proteínas Virales/biosíntesis , Animales , Transformación Celular Viral , Ratones , Peso Molecular , Biosíntesis de Proteínas , Ribosomas/metabolismoAsunto(s)
Adamantano/análogos & derivados , Hidrocarburos Aromáticos con Puentes/análogos & derivados , Células Cultivadas/efectos de los fármacos , Virus de la Influenza A/efectos de los fármacos , Biosíntesis de Péptidos , Adamantano/farmacología , Animales , Embrión de Pollo , Relación Dosis-Respuesta a Droga , Virus de la Influenza A/metabolismoAsunto(s)
Adamantano/farmacología , Hidrocarburos Aromáticos con Puentes/farmacología , Subtipo H1N1 del Virus de la Influenza A , Virus de la Influenza A/efectos de los fármacos , Adamantano/análogos & derivados , Animales , Embrión de Pollo , Perros , Relación Dosis-Respuesta a Droga , Riñón , Proteínas Virales/antagonistas & inhibidores , Cultivo de Virus , Replicación Viral/efectos de los fármacosAsunto(s)
Antivirales/farmacología , Virus de la Influenza A/efectos de los fármacos , Polirribosomas/efectos de los fármacos , ARN Nucleotidiltransferasas/metabolismo , ARN Polimerasa Dependiente del ARN/metabolismo , Ribavirina/farmacología , Ribonucleósidos/farmacología , Replicación Viral/efectos de los fármacos , Animales , Células Cultivadas , Embrión de Pollo , Depresión Química , Inducción Enzimática/efectos de los fármacos , ARN Viral/biosíntesis , Proteínas Virales/biosíntesisRESUMEN
An anti-influenza preparation, rimantadine (alpha-methyl-1-adamantane methylamine hydrochloride) at concentrations of 10--25 mkg/ml depresses the RNA-dependent RNA polymerase induction in a culture of cells infected with influenza virus (fowl plague virus). The inhibitory effect is also observed 2 hours following cell infection. In vitro studies have demonstrated that rimantadine has no effect on the activity of virus-induced RNA-dependent RNA polymerase, as well as on that of RNA-dependent RNA polymerase associated with virus particles.