Potential of ethno-veterinary medicine in animal health care practices in the South Pacific Island countries: a review.

The South Pacific island countries (SPIC) are among the most vulnerable countries in the world to economic shocks, climate change and natural disasters. Agriculture including livestock production makes important contribution to socio-economic development in the region. However, the livestock sector is marred by many livestock diseases, which are crippling the economies as well as threatening the food security of the island nations, most of which have high dependence on imports. Limited veterinary medical facilities, high cost of conventional medicine and remoteness of some rural farming communities make it difficult for farmers to treat their diseased animals. Nevertheless, the island nations are endowed with vast reserves of medicinal plants in diverse ecosystems including mangroves, rainforests and tropical dry forests. Despite the ubiquity of traditional medicine systems, most of the plant species have been used to treat human aliments in the SPIC and very few farmers utilise herbal medicine to treat their animals. This review highlights the common diseases affecting livestock production in the SPIC, threats to ethno-veterinary knowledge, and potential plant species and their efficacies to treat animal diseases and parasites. It also discusses the common methods of preparation of veterinary herbal medicine and possible ways of making the medicine available in the formal and informal markets in the region. It is envisaged that the review will stimulate further ethno-veterinary research among livestock disease management practices in the SPIC.

Phytoremediation of Potentially Toxic Elements: Role, Status and Concerns.

Environmental contamination with a myriad of potentially toxic elements (PTEs) is triggered by various natural and anthropogenic activities. However, the industrial revolution has increased the intensity of these hazardous elements and their concentration in the environment, which, in turn, could provoke potential ecological risks. Additionally, most PTEs pose a considerable nuisance to human beings and affect soil, aquatic organisms, and even nematodes and microbes. This comprehensive review aims to: (i) introduce potentially toxic elements; (ii) overview the major sources of PTEs in the major environmental compartments; (iii) briefly highlight the major impacts of PTEs on humans, plants, aquatic life, and the health of soil; (iv) appraise the major methods for tackling PTE-caused pollution; (v) discuss the concept and applications of the major eco-technological/green approaches (comprising phytoextraction, rhizofiltration, phytostabilization, phytovolatilization, and phytorestoration); (vi) highlight the role of microbes in phytoremediation under PTE stress; and (vii) enlighten the major role of genetic engineering in advancing the phytoremediation of varied PTEs. Overall, appropriate strategies must be developed in order to stop gene flow into wild species, and biosafety issues must be properly addressed. Additionally, consistent efforts should be undertaken to tackle the major issues (e.g., risk estimation, understanding, acceptance and feasibility) in order to guarantee the successful implementation of phytoremediation programs, raise awareness of this green technology among laymen, and to strengthen networking among scientists, stakeholders, industrialists, governments and non-government organizations.

Pattern of ß-Diversity and Plant Species Richness along Vertical Gradient in Northwest Himalaya, India.

The structure and distribution patterns of Himalayan vegetation are poorly explored, and research on species composition along the elevation gradient in these mountain ranges is still deficient. The current study was undertaken to analyze the variation and pattern of plant species composition along a vertical gradient in northwestern Himalaya, India. A total of 18 sites were selected along an elevation gradient ranging from 2200 to 3900 m asl positioned at an interval of 100 m. The Renyi diversity profile, non-metric multidimensional scaling based on the Bray-Curtis dissimilarity metric and beta diversity components among the elevation belts were calculated. Furthermore, to study the influence of altitude on species richness and diversity, a generalized additive model was created. Two hundred and ten plant species representing 66 families and 147 genera were recorded. The Renyi diversity profiles show that the lower and mid-altitudes had rich species diversity. The results of the non-metric multidimensional scaling analysis show a considerable variation in the total plant species composition among the studied elevation belts. The observed multiple-site Sorensen dissimilarity index across the studied elevation belts was very high. The contribution of species replacement or the turnover component to the observed dissimilarity was much higher than the nestedness component. Furthermore, the herbaceous and tree richness showed a significant decrease with increase in elevation; however, the richness of shrubs showed a bimodal pattern. The present study increases our understanding of the trends and patterns of species richness along the vertical gradient in the Himalayan region.

Implementation of the Use of Ethnomedicinal Plants for Curing Diseases in the Indian Himalayas and Its Role in Sustainability of Livelihoods and Socioeconomic Development.

In recent times, the use of traditional herbal medicines in healthcare has declined, particularly amongst the rural population. This implies a risk of losing vital information from previous generations regarding plants and their use in traditional medicine. The objective of this study is to catalog the ways employed by inhabitants of the Garhwal Himalayas as part of their traditional approaches to healthcare. Information was gathered through snowball sampling using a questionnaire combined with informal interviews. This was supplemented by discussions with peers and practitioners prominent in this healing technique. The homogeneity within ethnomedicinal knowledge of these rural residents was tested using the informant consensus factor (Fic). The calculation of the fidelity value (FL) and the cultural importance index (CI) were made regarding the population's dependency on plants. A total of 88 plant species from 44 families and 80 genera were identified as medicines for various complaints. Leaves were the most frequently used plant part followed by fruits, seeds, roots, bark, and flowers/buds. The largest number of taxa (15 species) were used for treatment of skin ailments (with Fic score of 0.85) followed by wounds, coughs, and digestive problems. There was a significant relationship observed between the medicinal plants used and distance (time of access) and family income. The present study will provide baseline information to be established for future research. The available information could help to discover new drugs for the pharmaceutical industry. Thus, the study revealed that the plants that have high scores of FL and CI can be used to discover new drug extraction in the future for further studies.

Real-world deployment of lateral flow SARS-CoV-2 antigen detection in the emergency department to provide rapid, accurate and safe diagnosis of COVID-19.

BACKGROUND: Point-of-care (POC) SARS-CoV-2 lateral-flow antigen detection (LFD) testing in the emergency department (ED) could inform rapid infection control decisions but requirements for safe deployment have not been fully defined. METHODS: Review of LFD test results, laboratory and POC-RT-PCR results and ED-performance metrics during a two-week high SARS-CoV-2 prevalence period followed by several months of falling prevalence. AIM: Determine whether LFD testing can be safely deployed in ED to provide an effective universal SARS-CoV-2 testing capability. FINDINGS: 93% (345/371) of COVID-19 patients left ED with a virological diagnosis during the 2-week universal LFD evaluation period compared to 77% with targeted POC-RT-PCR deployment alone, on background of approximately one-third having an NHS Track and Trace RT-PCR test-result at presentation. LFD sensitivity and specificity was 70.7% and 99.1% respectively providing a PPV of 97.7% and NPV of 86.4% with disease prevalence of 34.7%. ED discharge-delays (breaches) attributable to COVID-19 fell to 33/3532 (0.94%) compared with the preceding POC-RT-PCR period (107/4114 (2.6%); p=<0.0001). Importantly, LFD testing identified 1 or 2 clinically-unsuspected COVID-19 patients/day. Three clinically-confirmed LFD false positive patients were appropriately triaged based on LFD action-card flowchart, and only 5 of 95 false-negative LFD results were inappropriately admitted to non-COVID-19 areas where no onward-transmission was identified. LFD testing was restricted to asymptomatic patients when disease prevalence fell below 5% and detected 1-3 cases/week. CONCLUSION: Universal SARS-CoV-2 LFD testing can be safely and effectively deployed in ED alongside POC-RT-PCR testing during periods of high and low disease prevalence.

Ethnomedicinal knowledge of indigenous communities and pharmaceutical potential of rainforest ecosystems in Fiji Islands.

The World Health Organization recently raised concerns about antimicrobial resistance and lack of novel antibiotics in the health sector. The success rate of drug discovery is higher when chemical constituents are sourced from natural products and when drug candidates are based on the indigenous knowledge of local communities. Tropical rainforests are an important source of medicinal plants for traditional healthcare systems. The pharmaceutical industry also recognizes the potential of rainforests in novel drug development. However, habitat degradation and loss of traditional knowledge are endangering the healing powers of nature. The islands of Fiji have a rich cultural history of traditional medicine and a number of medicinal plants are sourced from the country's rainforest ecosystems. While deforestation and forest degradation are decimating unique rainforest biodiversity and reducing access to medicinal plants in the wild, inter-generational erosion of ethnobotanical knowledge is attributed to acculturation, rural-urban migration and their effects on the transmission of oral traditions from one generation to another. Under these conditions, plants may disappear before their therapeutic value is formally identified. This review summarizes the importance of traditional medicinal knowledge and the potential for drug discovery from the tropical rainforest ecosystems of Fiji. However, there are several challenges that need to be addressed to realize the true potential of ethnopharmacology in this country.

Floristic composition and utilization of ethnomedicinal plant species in home gardens of the Eastern Himalaya.

BACKGROUND: Home gardens are popular micro land-use system and are socioeconomically linked with people for their livelihood. In the foothill region of Eastern Himalaya, very less documentations are available on species richness of the home gardens, particularly on the ethnomedicinal plants. We assumed that the home garden owners of the study site are domesticating ethnomedicinal plants which are not easily accessible to them in the wild due to distant forest. This study was planned to explore and document the diversity and population status of ethnomedicinal plants in the home gardens along with its ethnomedicinal use. METHODS: The present study was conducted in the home gardens of Cooch Behar district of West Bengal from May 2017 to May 2018. A multidisciplinary approach like collection of plant specimen, interview with structured questionnaire for documenting the utilization pattern, and quadrat methods for population study was applied. We selected 150 study sites randomly in the village cluster. The owners of the gardens were the respondents for the household survey. The study documented diversity, population size, and medicinal uses of ethnomedicinal plant species identified by the garden owners growing or being grown in their gardens. RESULTS: A total of 260 plant species were reported, of which, 53 were utilized for different ethnomedicinal applications. These 53 species were represented by 35 families and 45 genera. Most of these ethnomedicinal species were woody perennials (37.73%). Cocus nucifera dominated the list with highest number of use followed by Hibiscus rosa-sinensis. The use value of the species varied from 0.006 to 0.53, while the fidelity value (%) ranged from 2.29 to 93.75%. The leaves of the plants were mostly used for ethnomedicinal applications (19 species) followed by fruits (12 species) and bark (9 species), and the least was the root (7 species). We documented 20 different ailments/diseases cured by using these plants. In some cases, more than one species are used to cure a disease or ailment. As many as 10 species were used to cure only stomach-related problems. Some more diseases like cough and cold and jaundice were treated using six and four species, respectively. CONCLUSION: This documented list of 260 plant species including 53 ethnomedicinal ones from the home gardens of the study area indicates that these gardens are key in maintaining diversity and source of healthcare system in agricultural dominant landscape. Documenting such ecological status and traditional applications becomes a prerequisite for developing conservation and management strategies of home gardens to be included in the mainstream conservation processes.

Ethnomedicinal plants traditionally used in health care practices by inhabitants of Western Himalaya.

ETHNOPHARMACOLOGICAL RELEVANCE: Inspite of tremendous advances made in allopathic medicine, herbal practice still plays an important role in management and curing various ailments in remote and rural areas of India. However, traditional knowledge on the use of medicinal plants is eroding day by day and there is a need to document such knowledge, before it is lost forever. The aim of the present study was to document the indigenous and traditional knowledge of medicinal plants used by local inhabitants in and around Kedarnath Wildlife Sanctuary of Indian Himalaya for the advancement of biomedical research and development. MATERIALS AND METHODS: The intensive field survey was carried out at three different altitudes of Kedarnath Wildlife Sanctuary (KWLS) and its adjoining areas. The inhabitants were interviewed about the local name of plants having ethno-medicinal values, plant parts used, mode of processing/application and preparation and dosage through discussions and semi structured questionnaires. RESULTS: A total of 97 medicinal plant species belonging to 52 families and 83 genera were reported for curing various ailments like fever, cough, cold, digestive disorders, constipation, menstrual disorders etc. Out of 97 plant species reported, 21 are rare or threatened. Literature review revealed that 11 out of the 97 plant species are reported with new therapeutic uses. The most frequently utilized plant part was the root/rhizome (33%) followed by leaf (27%). In some cases whole plant was utilized. A few medicinal plants had some veterinary uses also. CONCLUSION: The study provides comprehensive information about the eroding indigenous and traditional knowledge of medicinal plants used by local inhabitants in a part of Western Himalaya, India. The identification of the active ingredients of the plants used by the local people may provide some useful leads for the development of new drugs and such new approaches of traditional knowledge regarding medicinal plants and laboratory analysis might help pharmaceutical industry in new chapters for the wellbeing of mankind.

Extra pontine osmotic demyelination syndrome.

The osmotic demyelination syndrome (ODS) has been identified as a complication of the rapid correction of hyponatremia for decades. However, in recent years, a variety of other medical conditions have been associated with the development of ODS, independent of changes in serum sodium which cause a rapid changes in osmolality of the interstitial (extracellular) compartment of the brain leading to dehydration of energy-depleted cells with subsequent axonal damage that occurs in characteristic areas. Slow correction of the serum sodium concentration and additional administration of corticosteroids seems to be a major prevention step in ODS patients. In the current report we aimed to share a rare case which we observed in our hospital. A 65 year old female admitted as altered sensorium with history of vomiting, diarrhea was managed with intravenous fluids for 2 days at a peripheral health centre. Patient was referred to our centre with encephalopathy, evaluated and found to have hyponatremia and hypokalemia rest of biochemical parameters and septic profile were normal. Patient's electrolyte disturbances were managed as per guidelines but encephalopathy persisted. Supportive treatment was continued and patient was discharged after 2 wks of stay in hospital after gaining full sensorium and neurological functions.

Influence of olfactory stimulation on nociceptive behavior in mice.

The reactions elicited by nociceptive stimulations were studied in mice exposed to the presence of different odors: positive (attractive), negative (aversive), or neutral. In a first set of experiments, the animals were not habituated to the odors before the nociceptive stimuli were applied; in this case, the olfactory environment during experienced pain had essentially no effect on the nociceptive reactions, whatever the nature of the odors. In a second set of experiments, the animals were habituated to the same odor for 20 days. The control group consisted of mice habituated to and tested in the presence of the odor of the laboratory. In that case, compared to controls, the neutral odor had no influence on nociceptive reactions. By contrast, the positive odor decreased and the negative odor increased the reactions, especially when the intensity of the nociceptive stimulus was low. Moreover, it has been shown that the reactions elicited during a second nociceptive stimulation period depend on the perception of pain animals experience during the first stimulation, which depended, in turn, on the odor associated with it. Results are discussed in terms of opioid-mediated interactions between olfaction and pain.

Ecological status and traditional knowledge of medicinal plants in Kedarnath Wildlife Sanctuary of Garhwal Himalaya, India.

BACKGROUND: Himalayan forests are the most important source of medicinal plants and with useful species for the local people. Kedarnath Wildlife Sanctuary (KWLS) is situated in the interior part of the Garhwal Himalayan region. The presented study was carried out in Madhmeshwar area of KWLS for the ecological status of medicinal plants and further focused on the ethnomedicinal uses of these plants in the study area. METHODS: Ecological information about ethnomedicinal plants were collected using random quadrats in a random sampling technique along an altitudinal gradient in the KWLS. Information on medicinal properties of plants encountered in the present study was generated by questionnaire survey and was also compared with relevant literature. RESULTS: A total of 152 medicinally important plant species were reported, in which 103 were found herbs, 32 shrubs and 17 were tree species which represented 123 genera of 61 families. A total of 18 plant species fell into the rare, endangered (critically endangered) and vulnerable status categories. CONCLUSION: The present study documented the traditional uses of medicinal plants, their ecological status and importance of these plants in the largest protected area of Garhwal Himalaya. This study can serve as baseline information on medicinal plants and could be helpful to further strengthen the conservation of this important resource.