RESUMEN
Following a suspected case of hantavirus in a patientsuffering from acute kidney injury, rodents fromthe patient's property in Yorkshire and the Humber,United Kingdom (UK) were screened for hantaviruses.Hantavirus RNA was detected via RT-PCR in two Rattusnorvegicus. Complete sequencing and phylogeneticanalysis established the virus as a Seoul hantavirus,which we have provisionally designated as strainHumber. This is the first hantavirus isolated from wildrodents in the UK and confirms the presence of a pathogenicSeoul virus in Europe.
Asunto(s)
Lesión Renal Aguda/diagnóstico , Anticuerpos Antivirales/sangre , Infecciones por Hantavirus/epidemiología , ARN Viral/análisis , Virus Seoul/aislamiento & purificación , Lesión Renal Aguda/virología , Animales , Enfermedades Transmisibles Emergentes/virología , Reservorios de Enfermedades , Infecciones por Hantavirus/diagnóstico , Infecciones por Hantavirus/virología , Humanos , Masculino , Datos de Secuencia Molecular , Filogenia , ARN Viral/genética , Ratas , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Enfermedades de los Roedores , Virus Seoul/genética , Análisis de Secuencia de ADN , Reino Unido/epidemiologíaRESUMEN
A preliminary study was conducted to determine the presence of spotted fever rickettsiae in two species of British tick (Ixodes ricinus and Dermacentor reticulatus). The 16S rRNA gene of Rickettsia spp. was detected in 39/401 (9·7%) of ticks tested, including 22/338 (6·5%) I. ricinus and 17/63 (27%) D. reticulatus. Some positive I. ricinus samples showed 100% homology with Rickettsia helvetica (10/22), and most positive D. reticulatus showed 100% homology with R. raoultii (13/17). Five other Rickettsia spp. were detected exhibiting 96-99% homology. Ticks positive for rickettsiae were collected from various hosts and from vegetation from eight counties across Great Britain. The distribution of R. helvetica in various engorged and unfed stages of I. ricinus suggests that R. helvetica is widespread. R. raoultii was found in questing adult D. reticulatus in Wales and England. This is the first evidence of potentially pathogenic spotted fever rickettsiae in British ticks.
Asunto(s)
Dermacentor/microbiología , Ixodes/microbiología , Rickettsia/aislamiento & purificación , Animales , Fiebre Botonosa/microbiología , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Femenino , Masculino , Filogenia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido Nucleico , Reino Unido , Zoonosis/microbiologíaRESUMEN
Field studies were carried out to determine whether ticks are being imported into the British Isles on migratory birds. During spring and autumn migration 2004, ticks were collected from ringed birds at 11 bird observatories and 3 inland Riparia riparia colonies. A total of 38 ticks of 4 species (Ixodes ricinus, I. frontalis, I. lividus, I. arboricola) were collected from 12 species of bird. Ticks were tested for viruses in the Flavivirus and Nairovirus genera, with no positives found. This data demonstrates that ticks are being imported into the British Isles on migratory birds with future work recommended to determine the quantity of ticks imported and to detect low prevalence pathogens.