Concordant genotype of upper and lower airways P aeruginosa and S aureus isolates in cystic fibrosis.

RATIONALE: Lower airway (LAW) infection with Pseudomonas aeruginosa and Staphylococcus aureus is the leading cause of morbidity in cystic fibrosis (CF). The upper airways (UAW) were shown to be a gateway for acquisition of opportunistic bacteria and to act as a reservoir for them. Therefore, tools for UAW assessment within CF routine care require evaluation. OBJECTIVES: The aims of the study were non-invasive assessment of UAW and LAW microbial colonisation, and genotyping of P aeruginosa and S aureus strains from both segments. METHODS: 182 patients with CF were evaluated (age 0.4-68 years, median 17 years). LAW specimens were preferably sampled as expectorated sputum and UAW specimens by nasal lavage. P aeruginosa and S aureus isolates were typed by informative single nucleotide polymorphisms (SNPs) or by spa typing, respectively. RESULTS: Of the typable S aureus and P aeruginosa isolates from concomitant UAW- and LAW-positive specimens, 31 of 36 patients were carrying identical S aureus spa types and 23 of 24 patients identical P aeruginosa SNP genotypes in both compartments. Detection of S aureus or P aeruginosa in LAW specimens was associated with a 15- or 88-fold higher likelihood also to identify S aureus or P aeruginosa in a UAW specimen from the same patient. CONCLUSIONS: The presence of identical genotypes in UAW and LAW suggests that the UAW play a role as a reservoir of S aureus and P aeruginosa in CF. Nasal lavage appears to be suitable for non-invasive UAW sampling, but further longitudinal analyses and comparison with invasive methods are required. While UAW bacterial colonisation is typically not assessed in regular CF care, the data challenge the need to discuss diagnostic and therapeutic standards for this airway compartment. TRIAL REGISTRATION NUMBER: NCT00266474.

Gait with and without forearm crutches in patients with total hip arthroplasty.

There is considerable variation between centres in the use of forearm crutches in the rehabilitation of patients with total hip arthroplasty who are capable of full weight bearing. This study aimed to compare the gait of patients with total hip arthroplasty walking with and without crutches. The gait analysis in 19 patients included the assessment of kinematics, kinetics and the kinesiological electromyographic activity of lower limb and trunk muscles. With the forearm crutches patients walked with a reduced cadence, a longer stride length and more symmetrically (P < 0.05). The activity of the gluteus medius, vastus medialis and lateralis, and erector spinae muscles of the affected and of the vastus medialis muscle of the unaffected side decreased significantly (P < 0.05). Furthermore, seven subjects displayed an abnormal activation pattern of the affected hip abductor when walking with forearm crutches, characterized by a second burst during swing (n = 5) or a tonic pattern (n = 2). It is concluded that the use of forearm crutches resulted in a symmetrical gait pattern. The reduced activity of relevant pelvi-trochanteric muscles and the disturbed activation pattern of the affected hip abductor when walking with crutches might indicate that patients should walk unaided as soon as possible to provide a more efficient muscular training under dynamic conditions.

[Deterioration of clinical findings in hip joint follow-ups in neonatal screening]. / Befundverschlechterungen bei der Verlaufsbeobachtung von Hüftgelenken aus einem neonatalen Screening.

6 per cent out of 4290 hips checked by neonatal ultrasound screening could be reexamined later on. 7 of 200 hips (i.e. 4 per cent), primarily categorized as type IIa, showed an unexpected severe deterioration to type D (n = 3) or even to type IIIa (n = 4) after a mean-time of 9 weeks. Additional 5 cases with similar unfavourable courses from IIa to IIc, D or IIIa, and even 3 initially normal hips, changing to type IIa, were seen in another screening material (not yet systematically analyzed). Reasons for possible initial misinterpretation (purely visual evaluation in most cases, uncertainties in measuring suboptimal sonographic cuts, deficiencies in technical equipment and documentation), equivocal classification of border-line sonograms as well as possible influence of additional risk factors are discussed. Routine sonographic re-examinations after 6 (to 12) weeks are recommended as obligatory for all hips.

[The gait of patients with full weightbearing capacity after hip prosthesis implantation on the treadmill with partial body weight support, during assisted walking and without crutches]. / Das Gehen von Patienten mit voll belastbarem künstlichen Hüftgelenk auf dem Laufband mit partieller Körpergewichtsentlastung, im Kreuzgang und hilfsmittelfrei.

PROBLEM: Treadmill training with partial body weight support is a new promising technique in the rehabilitation in hip arthroplasty patients. With little data on the gait pattern and extent of pelvitrochanteric muscle activation, this study analysed the gait of hip arthroplasty patients walking on the treadmill and also during floor walking with and without crutches. METHOD: Gait analysis in 19 hip arthroplasty patients capable of full weight bearing included the assessment of kinematics, kinetics and kinesiologic electromyogram of relevant trunk and lower limb muscles during treadmill walking with 15% of body weight support, and during floor walking with and without crutches at comparable walking velocities. RESULTS: Both on the treadmill and with crutches, patients walked less frequent, with a longer stride and more symmetric as compared to the unaided gait. Both techniques, however, resulted in a reduced activation of most of the pelvitrochanteric muscles, particularly when using crutches. The comparison of both supporting methods revealed a higher activity of the M. gluteus medius of the affected side on the treadmill. The amount of body weight reduction was comparable ranging from 10 to 15%. CONCLUSIONS: Treadmill training with constant body weight support enables hip arthroplasty patient to entrain a dynamic and symmetric gait pattern with a better activation of the hip abductor of the affected side as compared to walking with crutches. Nevertheless, the level of activation was less than when walking without crutches which, however, resulted in a limping gait pattern.