Automated detection of estrous behavior in tie-stall housing using a barometer and accelerometer.

Tie-stall housing inhibits movement in cows, thereby restricting the behavioral indicators used by farmers for detecting estrous. In this study, we investigated the changes in patterns of lying and standing times at estrous, and evaluated the potential for automated detection of estrous within tie-stalls using a barometer and accelerometer. On estrous days, total daily standing time was significantly longer than that during non-estrous days (P < 0.05). A practical method was developed for detecting slight altitude changes using a novel device, which consisted of a barometer and accelerometer, and was attached to the neckband. Total daily standing time predicted using this new device was found to be highly correlative with the observed measured data (r = 0.95, P < 0.01), indicating the accuracy of the device in measuring daily standing time in tie-stall housed cows. In addition, the device detected an overall increase in total daily standing time during estrous days.

Peroxisome proliferator-activated receptor-α expression is associated with histological type in human gastric carcinoma.

Gastric carcinoma is one of the most common types of cancer worldwide and a leading cause of cancer-related mortality. Gastric carcinoma is histologically subdivided into differentiated and undifferentiated carcinoma, with the latter including poorly differentiated carcinoma and signet ring cell carcinoma (SRCC). Poorly differentiated carcinoma and SRCC have a worse prognosis compared with differentiated carcinoma. Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors and the PPAR-α subtype regulates important cellular functions, including cell proliferation, energy metabolism, oxidative stress, immune responses and cell differentiation. The aim of the present study was to elucidate the associations between clinicopathological factors and PPAR-α expression in patients with gastric carcinoma. The immunohistochemical staining of specimens obtained from 57 patients showed that PPAR-α expression was slightly weaker in undifferentiated carcinoma than in differentiated carcinoma (P<0.01). PPAR-α expression also significantly differed between poorly differentiated carcinoma (both positive and negative: 14/20, 70%) and SRCC (not expressed: 0/7, 0%) (P<0.01). However, PPAR-α expression was not significantly affected by age, lymph node invasion, venous invasion, lymph node metastasis, depth of invasion or stage. Collectively, the present results demonstrated that the downregulated expression of PPAR-α may play a key role in the biological transformation of tumors. Therefore, PPAR-α appears to be an important protein related to histology and may hold promise as a prognostic marker. Further studies with a larger number of subjects are needed to elucidate the relationship between PPAR-α expression and tumor progression and to analyze long-term clinical survival.