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1.
Vet Res ; 55(1): 38, 2024 Mar 26.
Artículo en Inglés | MEDLINE | ID: mdl-38532494

RESUMEN

A novel variant of rabbit haemorrhagic disease virus, designated RHDV2/b/GI.2, was first discovered in France in 2010. Subsequently, RHDV2 rapidly spread to Africa, North America, Australia, and Asia. RHDV2 outbreaks have resulted in significant economic losses in the global rabbit industry and disrupted the balance of natural ecosystems. Our study investigated the seasonal characteristics of RHDV2 outbreaks using seasonal indices. RHDV2 is prone to causing significant outbreaks within domestic and wild rabbit populations during the spring season and is more likely to induce outbreaks within wild rabbit populations during late autumn in the Southern Hemisphere. Furthermore, based on outbreak data for domestic and wild rabbits and environmental variables, our study established two MaxEnt models to explore the relationship between RHDV2 outbreaks and the environmental factors and conducted outbreak risk predictions for RHDV2 in global domestic and wild rabbit populations. Both models demonstrated good predictive performance, with AUC values of 0.960 and 0.974, respectively. Road density, isothermality, and population density were identified as important variables in the outbreak of RHDV2 in domestic rabbits, while road density, normalized difference vegetation index, and mean annual solar radiation were considered key variables in the outbreak of RHDV2 in wild rabbits. The environmental factors associated with RHDV2 outbreaks identified in our study and the outbreak risk prediction maps generated in our study will aid in the formulation of appropriate RHDV2 control measures to reduce the risk of morbidity in domestic and wild rabbits.


Asunto(s)
Infecciones por Caliciviridae , Virus de la Enfermedad Hemorrágica del Conejo , Animales , Conejos , Filogenia , Ecosistema , Infecciones por Caliciviridae/veterinaria , Medición de Riesgo
2.
Amino Acids ; 55(10): 1317-1331, 2023 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-37670010

RESUMEN

The emergence of drug-resistant superbugs has necessitated a pressing need for innovative antibiotics. Antimicrobial peptides (AMPs) have demonstrated broad-spectrum antibacterial activity, reduced susceptibility to resistance, and immunomodulatory effects, rendering them promising for combating drug-resistant microorganisms. This study employed computational simulation methods to screen and design AMPs specifically targeting ESKAPE pathogens. Particularly, AMPs were rationally designed to target the BamA and obtain novel antimicrobial peptide sequences. The designed AMPs were assessed for their antibacterial activities, mechanisms, and stability. Molecular docking and dynamics simulations demonstrated the interaction of both designed AMPs, 11pep and D-11pep, with the ß1, ß9, ß15, and ß16 chains of BamA, resulting in misfolding of outer membrane proteins and antibacterial effects. Subsequent antibacterial investigations confirmed the broad-spectrum activity of both 11pep and D-11pep, with D-11pep demonstrating higher potency against resistant Gram-negative bacteria. D-11pep exhibited MICs of 16, 8, and 32 µg/mL against carbapenem-resistant Escherichia coli, carbapenem-resistant Pseudomonas aeruginosa, and multi-drug-resistant Acinetobacter baumannii, respectively, with a concomitant lower resistance induction. Mechanism of action studies confirmed that peptides could disrupt the bacterial outer membrane, aligning with the findings of molecular dynamics simulations. Additionally, D-11pep demonstrated superior stability and reduced toxicity in comparison to 11pep. The findings of this study underscore the efficacy of rational AMP design that targets BamA, along with the utilization of D-amino acid replacements as a strategy for developing AMPs against drug-resistant bacteria.


Asunto(s)
Péptidos Catiónicos Antimicrobianos , Péptidos Antimicrobianos , Péptidos Catiónicos Antimicrobianos/farmacología , Péptidos Catiónicos Antimicrobianos/química , Simulación del Acoplamiento Molecular , Antibacterianos/química , Carbapenémicos , Pruebas de Sensibilidad Microbiana
3.
Small ; 18(41): e2203264, 2022 10.
Artículo en Inglés | MEDLINE | ID: mdl-36070429

RESUMEN

Superhydrophobic surfaces with the "lotus effect" have wide applications in daily life and industry, such as self-cleaning, anti-freezing, and anti-corrosion. However, it is difficult to reliably predict whether a designed superhydrophobic surface has the "lotus effect" by traditional theoretical models due to complex surface topographies. Here, a reliable machine learning (ML) model to accurately predict the "lotus effect" of solid surfaces by designing a set of descriptors about nano-scale roughness and micro-scale topographies in addition to the surface hydrophobic modification is demonstrated. Geometrical and mathematical descriptors combined with gray level cooccurrence matrices (GLCM) offer a feasible solution to the puzzle of accurate descriptions of complex topographies. Furthermore, the "black box" is opened by feature importance and Shapley-additive-explanations (SHAP) analysis to extract waterdrop adhesion trends on superhydrophobic surfaces. The accurate prediction on as-fabricated superhydrophobic surfaces strongly affirms the extensionality of the ML model. This approach can be easily generalized to screen solid surfaces with other properties.


Asunto(s)
Aprendizaje Automático , Modelos Teóricos , Interacciones Hidrofóbicas e Hidrofílicas , Propiedades de Superficie
4.
BMC Vet Res ; 16(1): 119, 2020 Apr 23.
Artículo en Inglés | MEDLINE | ID: mdl-32326962

RESUMEN

BACKGROUND: Hoof disease is one of the three major diseases that often occur in dairy cows. The impact of this disease on dairy farming is second only to mastitis. Laminitis is a diffuse, aseptic, serous, non-purulent inflammation of the dermal papillae and vascular layers of the cow's hoof wall. In the pasture, laminitis occurs mostly in the laminae, that is, inside the hoof shell. No lesions can be seen on the surface. Therefore, laminitis cannot attract the attention of veterinarians. However, laminitis has become a major factor that seriously affects the health and welfare of dairy cows, making it an important cause of hindering the performance of dairy cows. METHODS: The study was conducted at a dairy farm in Harbin, Heilongjiang province, China. We selected a sample of the laminitis cows based on the veterinary diagnosis, took blood from the jugular vein and then separated the plasma, and measured the index with the Elisa kit. In this study, the markers of inflammatory and vasoactive substances status in dairy cows consisted of subclinical laminitis (SCL, n = 20), chronic laminitis (CL, n = 20) and healthy dairy cows (CON, n = 20) under the local management conditions were investigated. RESULTS: Compared with healthy cattle, HIS, IL-6, LPS, and TNF-α in subclinical laminitis group significantly increased (P < 0.05), especially HIS, LPS, TNF-α (P < 0.01); in chronic laminitis cows, COX-2, HIS, IL-6, LPS, and TNF-α increased significantly (P < 0.05), especially COX-2, HIS, TNF-α (P < 0.01). iNOS (P < 0.05), TXB2 (P < 0.01) in chronic laminitis cows had significantly increased. CONCLUSION: This study reported for the first time that pasture laminitis was divided into subclinical laminitis and clinical chronic laminitis. Through research on the inflammatory factors and vasoactive substances of dairy cows, it is found that there is a close relationship between them, which affects the metabolic cycle of dairy cows. These indicators are abnormally expressed and cause hoof microcirculation disorders.


Asunto(s)
Citocinas/sangre , Pezuñas y Garras/patología , Mediadores de Inflamación/sangre , Animales , Bovinos , China , Industria Lechera , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Vasoconstrictores/sangre
5.
Endocr Pract ; 26(6): 642-650, 2020 Jun 02.
Artículo en Inglés | MEDLINE | ID: mdl-32045288

RESUMEN

Objective: Patients with DiGeorge syndrome (DGS) are undiagnosed due to its diverse manifestations. We aimed to characterize the clinical manifestations in a group of Chinese patients of DGS with childhood-onset hypoparathyroidism (HP) as the primary referral, and to report a novel TBX1 mutation. Methods: In this single-center observational study, clinical features and biochemical indices were recorded in 26 patients with DGS and 114 patients with idiopathic HP (IHP). An in vitro functional experiment was launched to analyze the novel TBX1 missense mutation. Results: Compared with 114 patients of IHP (19.1 [13.5, 27.3] years old), 26 patients of DGS (14.9 [10.4, 20.3] years old) had the following differences: an earlier onset age of hypocalcemia; higher levels of serum parathyroid hormone, with a similar disease course; and lower doses of vitamin D preparation therapy. Among the 26 patients of DGS, only 3 of them were clinically diagnosed as this syndrome prior to genetic testing. A total of 25 patients of DGS were verified to have a TBX1 deletion and 1 case with a novel missense mutation of TBX1. The novel p.Y490C mutation in TBX1, located in the transactivation domain, was verified to decrease the transcriptional activity of the TBX1 protein. Conclusion: In this Chinese group of patients with DGS-related HP, a relatively earlier onset age and less severity of HP were found compared to that of patients with IHP. Less common extraparathyroid manifestations are clues for the diagnosis of DGS. Additionally, our discovery of a novel TBX1 missense mutation expands the mutation database of DGS. Abbreviations: DGS = DiGeorge syndrome; HP = hypoparathyroidism; IHP = idiopathic hypoparathyroidism; LCR = low copy repeat; PCR = polymerase chain reaction; PTH = parathyroid hormone; TAD = transactivation domain.


Asunto(s)
Síndrome de DiGeorge , Hipoparatiroidismo , Adulto , Niño , Síndrome de DiGeorge/complicaciones , Humanos , Hipoparatiroidismo/etiología , Proteínas de Dominio T Box , Adulto Joven
6.
Opt Express ; 27(6): 8808-8818, 2019 Mar 18.
Artículo en Inglés | MEDLINE | ID: mdl-31052693

RESUMEN

We demonstrate a practical method that is used to generate on-demand first- and higher-order cylindrical vector beams, in the 1550 nm band, directly from an all polarization maintaining mode-locked Er-fiber laser. On demand typical 1st order CVBs, including the radially and azimuthally polarized beams, can be easily achieved by properly adjusting the angle of a half-wave plate with respect to the fast axis of the vortex wave plate. The spatial beam mode can be flexibly switched with no disturbance on the time domain mode-locking output. The laser outputs the desired vector beams at 1571 nm with a spectral bandwidth at full-width at half-maximum of 32 nm. The mode-locked laser pulses have a repetition rate of 74.9 MHz. Moreover, the proposed method can be easily extended to create higher-order CVBs. Our research provides a convenient way to generate ultrafast pulses in highly flexible-controlled structured modes, which is essential for optical fabrication and light trapping applications.

7.
Opt Express ; 26(22): 28302-28311, 2018 Oct 29.
Artículo en Inglés | MEDLINE | ID: mdl-30470004

RESUMEN

We demonstrate an all polarization-maintaining (PM) fiber based dual-wavelength mode-locked Er-fiber laser. A nonlinear amplifying loop mirror (NALM) with an intracavity nonreciprocal phase shifter is used for self-started mode-locking. A short segment of PM fiber is angle-spliced to the NALM, functioning as a PM Sagnac loop filter, thus enabling dual-wavelength mode-locking. The wavelength separation is solely determined by the angle-spliced PM fiber length. Stable dual-wavelength mode-locking operation is switchable between 1570/1581 nm and 1581/1594 nm. The two-color pulse trains oscillating in the same cavity have an inherent offset repetition rate of ~1 kHz owing to cavity dispersion, allowing future high precision dual-comb applications with a simple and robust configuration.

8.
Ecotoxicol Environ Saf ; 110: 129-35, 2014 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-25232990

RESUMEN

Phytoremediation is an attractive approach for the cleanup of polycyclic aromatic hydrocarbons-contaminated soil. The joint effect of alfalfa and microorganisms, including Arthrobacter oxydans, Staphylococcus auricularis and Stenotrophomonas maltophilia, on pyrene removal was investigated. The results showed that the joint effect primarily contributed to pyrene removal, and the concentration of residual pyrene in rhizosphere soil was lower than that in non-rhizosphere soil. After joint treatment for 45d, pyrene in rhizosphere soils decreased from 11.3, 52.5 and 106.0mg/kg to 2.0-3.0, 15.0-18.7, and 41.2-44.8mg/kg, respectively. These bacteria significantly enhanced pyrene accumulation and microbial community diversity, and increased soil dehydrogenase and polyphenol oxidase activities. Pyrene was initially degraded through ring cleavage. One of the main metabolites 4-dihydroxy-phenanthrene was transformed into naphthol and 1,2-dihydroxynaphthalene, which were further degraded through salicylic acid pathway and phthalic acid pathway, separately.


Asunto(s)
Arthrobacter/metabolismo , Medicago sativa/química , Pirenos/química , Pirenos/metabolismo , Microbiología del Suelo , Contaminantes del Suelo/metabolismo , Staphylococcus/metabolismo , Stenotrophomonas maltophilia/metabolismo , Biodegradación Ambiental
9.
PLoS Negl Trop Dis ; 18(1): e0011884, 2024 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-38236812

RESUMEN

Against the backdrop of a global malaria epidemic that remains severe, China has eradicated indigenous malaria but still has to be alert to the risk of external importation. Understanding the distribution of vectors can provide an adequate and reliable basis for the development and implementation of vector control strategies. However, with the decline of malaria prevalence in recent years, the capacity of vector monitoring and identification has been greatly weakened. Here we have used new sampling records, climatic data, and topographic data to establish ecological niche models of the three main malaria vectors in China. The model results accurately identified the current habitat suitability areas for the three species of Anopheles and revealed that in addition to precipitation and temperature as important variables affecting the distribution of Anopheles mosquitoes, topographic variables also influenced the distribution of Anopheles mosquitoes. Anopheles sinensis is the most widespread malaria vector in China, with a wide region from the northeast (Heilongjiang Province) to the southwest (Yunnan Province) suitable for its survival. Suitable habitat areas for Anopheles lesteri are concentrated in the central, eastern, and southern regions of China. The suitable habitat areas of Anopheles minimus are the smallest and are only distributed in the border provinces of southern China. On this basis, we further assessed the seasonal variation in habitat suitability areas for these three major malaria vectors in China. The results of this study provide new and more detailed evidence for vector monitoring. In this new era of imported malaria prevention in China, regular reassessment of the risk of vector transmission is recommended.


Asunto(s)
Anopheles , Malaria , Animales , Malaria/epidemiología , Malaria/prevención & control , Estaciones del Año , Mosquitos Vectores , China/epidemiología , Ecosistema
10.
Discov Med ; 36(183): 827-835, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38665030

RESUMEN

OBJECTIVES: There are few follow-up studies on thyroid function in the same group for many years. Therefore, the purpose of this study was to retrospectively analyze the changes of thyroid function in a group of people for 8 years and to explore the changes of thyroid function in elderly men with normal thyroid function with age. METHODS: Reviewing the records of elderly men who underwent physical examination in the Beijing Hospital physical examination center from 2013 to 2020, 354 subjects were included in the study. According to age, they are divided into 4 groups. The differences in thyrotropin (TSH), anti-triiodothyronine (rT3), free triiodothyronine (FT3), and free thyroid hormone (FT4) among different age groups in initial time (2013) were compared. Longitudinal comparison of changes of thyroid function in the same age group for 8 years was compared too. RESULTS: At the initial time, age was negatively correlated with FT3 (r = 0.349, p < 0.001), positively correlated with rT3 and TSH (r = 0.182, p < 0.001, r = 0.212, p < 0.001), but not correlated with FT4. The results of eight years of analysis show that, for TSH, during the whole follow-up period, the TSH of the >80 years group was higher than that of the <60 years and 60-69 years groups, and the difference was statistically significant. The 70-79 age group was higher than the <60 years group at different time points, except for the age group <60 years. The other three groups showed an increasing trend with age, especially in the group of ≥80 years. For FT3, in 2013, the age ≥80 years group was significantly lower than that of the 70-79 years, 60-69 years, and <60 years old groups (p < 0.05). The analysis results at different time points in each age group showed a downward trend and then an upward trend. For FT4, there was no significant difference in FT4 among different age groups in 2013. Still, during the follow-up period, the age group ≥80 was lower than other age groups in 2019 and lower than the <60 years groups in 2014, 2015, 2019, and 2020, and the difference was statistically significant. The change rule of FT4 with the increase of age was not clear. For rT3, during the whole follow-up period, the rT3 of the >80 years group was higher than that of the <60 years and 60-69 years groups, and the difference was statistically significant. The analysis results at different time points in each age group showed a trend of rising first, then falling, and finally rising. After 2017, the rT3 of the 70-79 years and ≥80 years groups increased with age. CONCLUSIONS: The thyroid function index of elderly men changes with age. In transverse analysis, the value of TSH is the highest, and FT3 is the lowest in the group ≥80 years old. There are differences between the changes in the longitudinal analysis and the results of the horizontal analysis. Therefore, the law of thyroid function changing with age in different individuals is not the same as that of the same individual with age, which should be paid more attention in medical research and clinical diagnosis and treatment.


Asunto(s)
Envejecimiento , Pruebas de Función de la Tiroides , Glándula Tiroides , Tirotropina , Triyodotironina , Humanos , Masculino , Anciano , Glándula Tiroides/fisiología , Estudios Longitudinales , Envejecimiento/fisiología , Anciano de 80 o más Años , Triyodotironina/sangre , Tirotropina/sangre , Estudios Retrospectivos , Persona de Mediana Edad , Tiroxina/sangre , Factores de Edad
11.
Animal ; 18(3): 101085, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38364655

RESUMEN

The purpose of this study was to analyze the characteristics of occurrence and spread of highly pathogenic avian influenza H5N1 (HPAI-H5N1) globally, understand its spatiotemporal characteristics, investigate the risk factors influencing outbreaks, and identify high-risk areas for disease occurrence. We collected the data on global poultry HPAI-H5N1 outbreaks from January 2005 to April 2023, and conducted a thorough analysis of the spatial and temporal characteristics of the disease through time series decomposition and directional distribution analysis. Additionally, an ecological niche model was established to explore the major factors influencing the occurrence of HPAI-H5N1 and to pinpoint high-risk areas. Our findings revealed that HPAI-H5N1 outbreaks were cyclical, and seasonal, exhibiting a rising trend, with a predominant northwest-southeast transmission direction. The ecological niche model highlighted that species factors and economic trade factors are critical in influencing the outbreak of HPAI-H5N1. Variables such as chicken and duck density, population density, isothermality, and road density, contributed to importantly risk of outbreaks. High-risk areas for HPAI-H5N1 occurrence were primarily identified in Europe, West Africa, Southeast Asia, and Southeast China. This study provided valuable insights into the spatial and temporal distribution characteristics and risk factors of global poultry HPAI-H5N1 outbreaks. The identification of high-risk areas provides essential information that can be used to develop more effective prevention and control policies.


Asunto(s)
Subtipo H5N1 del Virus de la Influenza A , Gripe Aviar , Enfermedades de las Aves de Corral , Animales , Gripe Aviar/epidemiología , Gripe Aviar/prevención & control , Aves de Corral , Brotes de Enfermedades/veterinaria , Factores de Riesgo , Enfermedades de las Aves de Corral/epidemiología
12.
Free Radic Biol Med ; 213: 430-442, 2024 03.
Artículo en Inglés | MEDLINE | ID: mdl-38301977

RESUMEN

Hepatic ischemia-reperfusion injury (IRI) results in significant postoperative liver dysfunction, and the intricate mechanism of IRI poses challenges in developing effective therapeutic drugs. Mitigating the damage caused by hepatic IRI and promoting the repair of postoperative liver injury have become focal points in recent years, holding crucial clinical significance. Adipose mesenchymal stem cell derived exosomes (ADSCs-Exo) and metformin (Met) can play a mitochondrial protective role in the treatment of hepatic IRI, but whether there is a synergistic mechanism for their intervention is not yet known. Combining the unique advantages of exosomes as drug carriers, the aim of this study was to investigate the protective effects and mechanisms of the constructed Met and ADSCs-Exo complex (Met-Exo) on the liver IRI combined with partial resection injury in rat and hypoxic reoxygenation injury of rat primary hepatocytes (HCs). In this study, firstly, we detected that mitochondrial morphology and function were severely affected in hepatic tissues after hepatic IRI combined with partial resection, and then verified by in vitro experiments that Met-Exo could promote mitochondrial biosynthesis and fusion-associated protein expression and inhibit mitochondrial fission-related protein expression by modulating the AMPK/SIRT1 signalling pathway. This indicates that ADSCs-Exo can not only play a targeting role as a drug carrier but also has a great potential to act as a vehicle to act synergistically with drugs in the treatment of tissue and organ damage, which provides a new therapeutic strategy and experimental basis for the treatment of liver injury in medical science and clinical veterinary.


Asunto(s)
Metformina , Enfermedades Mitocondriales , Daño por Reperfusión , Ratas , Animales , Proteínas Quinasas Activadas por AMP/genética , Proteínas Quinasas Activadas por AMP/metabolismo , Metformina/farmacología , Sirtuina 1/genética , Sirtuina 1/metabolismo , Hígado/metabolismo , Daño por Reperfusión/tratamiento farmacológico , Daño por Reperfusión/genética , Daño por Reperfusión/metabolismo , Transducción de Señal , Enfermedades Mitocondriales/metabolismo
14.
Parasit Vectors ; 16(1): 297, 2023 Aug 26.
Artículo en Inglés | MEDLINE | ID: mdl-37633953

RESUMEN

BACKGROUND: Mosquitoes play an absolute role in the spread of epidemic arbovirus diseases. Worldwide, Aedes aegypti and Aedes albopictus are the main vectors responsible for the spread of these mosquito-borne diseases. Aedes vexans, a mosquito species native to China, also carries mosquito-borne viruses, such as dengue fever virus and Japanese encephalitis virus, but research on this mosquito has been inadequate. Mapping the potential distribution range of and monthly change in the distribution of these three Aedes species is of particular importance for mosquito surveillance, eradication and disease control. METHODS: Monitoring data were collected for the three Aedes species in China. Long-term temperature and precipitation data (2001-2021) and land cover data were used to represent various climate and environmental conditions. An ecological niche model was developed using a maximum entropy modeling method to predict the current optimum habitat areas for the three Aedes species and to obtain important variables influencing their monthly distribution. RESULTS: The distribution model for the three Aedes species performed well, with an area under the receiver operating characteristic curve value of 0.991 for Ae. aegypti, 0.928 for Ae. albopictus and 0.940 for Ae. vexans. Analysis of the distribution change and mapping of the optimum habitat range for each Aedes species for each month demonstrated that temperature, precipitation and construction land were important factors influencing the distribution of these three Aedes species. CONCLUSIONS: In China, Aedes aegypti is mainly concentrated in a few tropical regions and along the Yunnan border; Aedes albopictus is widely distributed throughout most of the country, except for the arid and semi-arid regions of northwest China; and Aedes vexans is mainly found in the northern regions. Our results provide a basis for the timing and location of surveillance efforts for high-priority mosquitoes.


Asunto(s)
Aedes , Virus de la Encefalitis Japonesa (Especie) , Animales , China , Mosquitos Vectores , Clima Desértico
15.
Sci Rep ; 12(1): 15614, 2022 09 16.
Artículo en Inglés | MEDLINE | ID: mdl-36114368

RESUMEN

African swine fever (ASF) is a tick-borne infectious disease initially described in Shenyang province China in 2018 but is now currently present nationwide. ASF has high infectivity and mortality rates, which often results in transportation and trade bans, and high expenses to prevent and control the, hence causing huge economic losses and a huge negative impact on the Chinese pig farming industry. Ecological niche modeling has long been adopted in the epidemiology of infectious diseases, in particular vector-borne diseases. This study aimed to establish an ecological niche model combined with data from ASF incidence rates in China from August 2018 to December 2021 in order to predict areas for African swine fever virus (ASFV) distribution in China. The model was developed in R software using the biomod2 package and ensemble modeling techniques. Environmental and topographic variables included were mean diurnal range (°C), isothermality, mean temperature of wettest quarter (°C), precipitation seasonality (cv), mean precipitation of warmest quarter(mm), mean precipitation of coldest quarter (mm), normalized difference vegetation index, wind speed (m/s), solar radiation (kJ /day), and elevation/altitude (m). Contribution rates of the variables normalized difference vegetation index, mean temperature of wettest quarter, mean precipitation of coldest quarter, and mean precipitation of warmest quarter were, respectively, 47.61%, 28.85%, 10.85%, and 7.27% (according to CA), which accounted for over 80% of contribution rates related to variables. According to model prediction, most of areas revealed as suitable for ASF distribution are located in the southeast coast or central region of China, wherein environmental conditions are suitable for soft ticks' survival. In contrast, areas unsuitable for ASFV distribution in China are associated with arid climate and poor vegetation, which are less conducive to soft ticks' survival, hence to ASFV transmission. In addition, prediction spatial suitability for future ASFV distribution suggests narrower areas for ASFV spread. Thus, the ensemble model designed herein could be used to conceive more efficient prevention and control measure against ASF according to different geographical locations in China.


Asunto(s)
Virus de la Fiebre Porcina Africana , Fiebre Porcina Africana , Fiebre Porcina Africana/epidemiología , Fiebre Porcina Africana/prevención & control , Algoritmos , Animales , China/epidemiología , Ecosistema , Porcinos
16.
Colloids Surf B Biointerfaces ; 201: 111653, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33667866

RESUMEN

Blood compatibility is an eternal topic of biomedical materials. The effect of heparin-mimicking polymers (HMPs) on blood compatibility has been well studied, especially the synergistic effect of sugar unit and sulfonate/sulfate unit. However, carboxylic groups also play an important role in HMPs. In this work, copolymers of sodium 4-vinyl-benzenesulfonate (SS) and 2-methacrylamido glucopyranose (MAG) (poly(SS-co-MAG)) and poly(acrylate acid) (PAA) were self-assembled on Au surfaces with different feed ratios. When self-assembly of poly(SS-co-MAG) alone, the optimized feed ratio of SS and MAG for vascular cell selectivity was 1:1 (PS1M1); at this ratio the Au-PS1M1 surface showed the highest human umbilical vein endothelial cells (HUVECs) density and the lowest human umbilical vein smooth muscle cells (HUVSMCs) density. When self-assembly of PAA alone (surface designated as Au-PAA), the proliferation of both HUVECs and HUVSMCs was inhibited. Compared with either PS1M1 or PAA alone, the surfaces modified with both PAA and PS1M1 at the feed ratio of 1:1 (material designated as Au-PSM/PAA-2) showed enhanced promoting effect on HUVECs as well as enhanced inhibiting effect on HUVSMCs, indicating stronger vascular cell selectivity of carboxylic groups in the presence of sugar and sulfonate units.


Asunto(s)
Heparina , Polímeros , Materiales Biocompatibles/farmacología , Células Endoteliales de la Vena Umbilical Humana , Humanos , Miocitos del Músculo Liso , Propiedades de Superficie
17.
Ying Yong Sheng Tai Xue Bao ; 32(1): 211-221, 2021 Jan.
Artículo en Zh | MEDLINE | ID: mdl-33477229

RESUMEN

Water shortage and soil salinization are two main limiting factors for cotton production in southern Xinjiang. We examined the effects of soil matrix potential (SMP) regulation at various growth stages on cotton growth, soil water and salt distribution, to provide theoretical basis for water saving, salts control, and efficient production in cotton fields. The mulched drip irrigation experiments were conducted to monitor cotton growth, aboveground biomass, cotton yield, soil water and salt distribution and other indicators. Three SMP thresholds, i.e.,W1(-20 kPa), W2(-30 kPa) and W3(-40 kPa) were set at the seedling stage (A), seedling stage + budding stage (B), and seedling stage + budding stage + flowering stage (C), with SMP of -50 kPa at 20 cm soil depth below the emitter as the CK. The results showed that plant height, leaf area index (LAI) and aboveground biomass followed the order of WC>WB>WA>CK, when SMP were changed at various growth stages. Plant height, LAI and aboveground biomass increased with increasing SMP thresholds, with the values under W1C and W2C being significantly higher than the other treatments. The effective bolls per plant, single boll weight and lint percentage all increased with the increases of SMP thresholds. The yields of W1C and W2C were similar, which were significantly higher than those of other treatments. However, the water use efficiency of W2C was significantly higher than that of W1C. Controlling the SMP threshold at -20 or -30 kPa at different growth stages could improve soil moisture status of the primary cotton root zone. All treatments presented shallow salt accumulation (0-40 cm) at the harvest stage, with the bare land having greater salt accumulation than the inner film. The higher the SMP threshold was, the less salt was accumulated in the primary root zone (0-40 cm) under film. The salt accumulation (0-40 cm) under W1C and W2C were reduced by 24% compared with other treatments. Considering the efficient production of cotton, water saving and salt control, it was recommended to maintain the SMP threshold of -30 kPa during irrigation at various growth stages in cotton fields without leaching salts during the local off-crop period.


Asunto(s)
Suelo , Agua , Riego Agrícola , Biomasa , Hojas de la Planta/química , Agua/análisis
18.
J Colloid Interface Sci ; 603: 501-510, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34197993

RESUMEN

Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure. In this study, the effects of heparin-like polymers and lotus leaf-like topography on surface vascular cell behavior are considered. By combining multicomponent thermo-curing and replica molding, a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography is obtained. Heparin-like polymers with different chemical compositions are grafted onto PDMS-Br surfaces using visible-light-induced graft polymerization. Compared with unmodified PDMS-Br, surfaces modified by sulfonate-containing polymers are more friendly to vascular cells, while those modified by a glyco-polymer are much more resistant to vascular cells. The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces. In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins. The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.


Asunto(s)
Polímeros , Adsorción , Heparina , Siliconas , Propiedades de Superficie
19.
PLoS One ; 15(5): e0232756, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32407323

RESUMEN

Mitogen-activated protein kinase (MAPK) is a form of serine/threonine protein kinase that activated by extracellular stimulation acting through the MAPK cascade (MAPKKK-MAPKK-MAPK). The MAPK cascade gene family, an important family of protein kinases, plays a vital role in responding to various stresses and hormone signal transduction processes in plants. In this study, we identified 14 CmMAPKs, 6 CmMAPKKs and 64 CmMAPKKKs in melon genome. Based on structural characteristics and a comparison of phylogenetic relationships of MAPK gene families from Arabidopsis, cucumber and watermelon, CmMAPKs and CmMAPKKs were categorized into 4 groups, and CmMAPKKKs were categorized into 3 groups. Furthermore, chromosome location revealed an unevenly distribution on chromosomes of MAPK cascade genes in melon, respectively. Eventually, qRT-PCR analysis showed that all 14 CmMAPKs had different expression patterns under drought, salt, salicylic acid (SA), methyl jasmonate (MeJA), red light (RL), and Podosphaera xanthii (P. xanthii) treatments. Overall, the expression levels of CmMAPK3 and CmMAPK7 under different treatments were higher than those in control. Our study provides an important basis for future functional verification of MAPK genes in regulating responses to stress and signal substance in melon.


Asunto(s)
Cucumis melo/enzimología , Cucumis melo/genética , Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Estudio de Asociación del Genoma Completo , Sistema de Señalización de MAP Quinasas/genética , Acetatos/farmacología , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Cromosomas de las Plantas/genética , Cucumis melo/efectos de los fármacos , Ciclopentanos/farmacología , Sequías , Exones/genética , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Genes de Plantas , Intrones/genética , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Proteínas Quinasas Activadas por Mitógenos/química , Proteínas Quinasas Activadas por Mitógenos/genética , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Oxilipinas/farmacología , Filogenia , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/genética , Dominios Proteicos , Ácido Salicílico/farmacología , Plantones/efectos de los fármacos , Plantones/enzimología , Plantones/genética , Cloruro de Sodio/farmacología , Estrés Fisiológico/efectos de los fármacos , Estrés Fisiológico/genética
20.
J Mater Chem B ; 2020 Sep 18.
Artículo en Inglés | MEDLINE | ID: mdl-32945818

RESUMEN

Heparin-like polymers are promising synthetic materials with biological functionalities, such as anticoagulant ability, growth factor binding to regulate cellular functions, and inflammation mediation, similar to heparin. The biocompatibility of heparin-like polymers with well-defined chemical structures has inspired many researchers to design heparin-like surfaces to explore their biological applications. The concept of the recombination of functional heparin structural units (sulfonate- and glyco-containing units) was proven to be successful in designing heparin-mimicking surfaces. However, besides surface structural units, topographic patterning is also an important contributor to the biological activity of the surfaces modified with heparin-like polymers. In this work, both surface structural units and topographic patterning were taken into account to investigate the vascular cell behaviors on the silicone surfaces. A facile method for the production of patterned bromine-containing polydimethylsiloxane surface (PDMS-Br) was developed from a one-step multicomponent thermocuring procedure and replica molding using a nanohole-arrayed silicon template. Different structural units of heparin-like polymers, i.e. homopolymer of sulfonate-containing sodium 4-vinylbenzenesulfonate (pSS), homopolymer of glyco-containing 2-(methacrylamido)glucopyranose (pMAG), and copolymers of MAG and SS (pSG), were then introduced on the flat and patterned PDMS-Br surface using visible light-induced graft polymerization. For the flat surfaces, compared with the PDMS-Br surface, pSS-grafted and pSG-grafted surfaces significantly increased cell densities of both human umbilical vein endothelial cells (HUVECs) and human umbilical vein smooth muscle cells (HUVSMCs), indicating that they are "vascular cell-friendly". In contrast, the pMAG-grafted surface showed decreased cell attachment of both HUVECs and HUVSMCs, indicating that the pMAG-grafted surface is "vascular cell-resistant". Moreover, surface topographic patterning enhanced the cell responses to the corresponding flat surfaces. That is to say, surface patterning can make the "vascular cell-friendly" surface still friendly, and the "vascular cell-resistant" surface much more resistant. The combination of surface structural units and topographic patterning shows promise in the preparation of new heparin-like surfaces with improved cell compatibility that is suitable for blood-compatible biomaterials.

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