Application of sodium bicarbonate in extraction and determination of synthetic colorant in processed grain products.

As the only standard of its kind, GB5009.35-2016 provides the determination of water-soluble synthetic colorants in processed grain products with high starch content for the purpose of food safety risk monitoring. However, it's only applicable to candy products and liquid foods as beverages, but not solid grain products. Extraction is a critical and essential step in the overall analytical process for determination. This paper provides an improved method for extraction of synthetic colorants in food products presenting high starch content. The samples were successively extracted with methanol-water (4:6, v/v) containing 2.7% sodium bicarbonate, and the target analytes were purified by solid phase extraction column. The obtained eluent was concentrated in constant volume, separated by ODS-SP C18 column and determined by diode array detector. The limits of detection were in the range of 2.21 ~ 8.62 ng/mL for 6 synthetic colors. The average recoveries at the spiked levels of 10, 30, 50 µg/kg varied in the range of 79.3 ~ 101.4% with RSD (n = 6) around 0.2 ~ 6.7%. The developed sodium bicarbonate based extraction method was successfully applied to speciation analysis of water soluble azo synthetic colorant in starchy food, such as millet, grits, brown rice, rice flour, cornmeal and cornflakes. Supplementary Information: The online version contains supplementary material available at 10.1007/s13197-021-05199-x.

Supercontinuum generation of 314.7 W ranging from 390 to 2400 nm by tapered photonic crystal fiber.

To consider both high-power handling and blue-extended supercontinuum (SC) generation, a long-tapered photonic crystal fiber is pumped by a high-power laser source. An SC ranging from 390 to 2400 nm with 314.7 W output power is obtained. A spectral component below 960 nm accounts for 36.1% of the total output power, exceeding 113.5 W, with a spectral flatness within 16 dB. To the best of our knowledge, this is the first time an SC coverage of all visible wavelengths with more than 300 W output power has been achieved. This result increases the output power of the SC covering the visible range by a factor of three.

High-power femtosecond cylindrical vector beam optical parametric oscillator.

We report on high-power femtosecond cylindrical vector beam (CVB) generation from a Gaussian-pumped optical parametric oscillator (OPO). By introducing a half waveplate and a vortex half-wave plate of m = 1 to realize intracavity polarization modulation to the resonant Gaussian signal, the OPO could deliver broadband signal beam in CVB profile, i.e., radially and azimuthally polarized beam profile. The central wavelength of the generated CVB signals can be tuned continuously from 1405 to 1601 nm, while the corresponding pulse durations are all around 150 fs. A maximum average output power of 614 mW at 1505 nm is obtained. Moreover, our OPO cavity design can be extended to generate high order CVB by simply changing the vortex half-wave plate with different orders. Such a high-power CVB OPO configuration has the advantages of flexible control and wide tuning range, making it a practical tool for applications in super-resolution imaging, optical communication and quantum correlations.

Pulse dynamics manipulation by the phase bias in a nonlinear fiber amplifying loop mirror.

Pulse dynamics controlling is of great importance for high quality pulse generation in ultra-short pulse fiber lasers. The pulse quality characteristics in terms of pulse duration, energy, chirp profile, tunability, as well as noise feature substantially depend on intracavity pulse propagation dynamics. Here we found that a nonlinear amplifying loop mirror mode-locked thulium-doped fiber laser can switch among enabling operation conventional soliton, stretched-pulse soliton and dissipative soliton regimes only by manipulating an intracavity phase bias device. This provides a simple approach to tailoring ultra-short laser characteristics to different applications.

Active f-to-2f interferometer for record-low jitter carrier-envelope phase locking.

The f-to-2f interferometer plays a key role for carrier-envelope phase (CEP) measurement and subsequent stabilization. The CEP measurement typically relies on the application of two optical nonlinearities, namely supercontinuum generation and second-harmonic generation. Then the cascadation of these nonlinearities often leads to signal levels on the order of a few photons per pulse. We experimentally demonstrate that the introduction of optical gain into the infrared arm of an f-to-2f interferometer can mitigate this detection bottleneck and improve signal-to-noise ratios by 20 dB compared to purely passive schemes. We further show that this measure allows for residual phase jitters between the carrier and envelope of about 10 mrad, corresponding to record-breaking timing jitters in the single attosecond regime. Moreover, the method appears generally applicable to a wide range of oscillators in the near-infrared and may enable stable CEP locking of mode-locked oscillators that so far have resisted stabilization. Finally, we propose a parametric variant of the active f-to-2f interferometer that can be used for laser amplifiers with kilohertz repetition rates and below.

Dual-comb spectroscopy with a single free-running thulium-doped fiber laser.

We demonstrate dual-comb spectroscopy in the vicinity of 2 µm wavelength based on a single dual-wavelength dual-comb Thulium-doped fiber laser. The shared laser cavity ensures passively maintained mutual coherence between the two combs due to common mode environmental noise rejection. In a proof-of-principle experiment, the absorption characteristics caused by the water in the optical path that composes the dual-comb spectrometer are measured. The retrieved spectral positions of the water absorption dips match with the HITRAN database.

Dielectric-mirror-less femtosecond optical parametric oscillator with ultrabroad-band tunability.

We demonstrate a high average power, widely tunable, dielectric-mirror-less optical parametric oscillator (OPO) based on MgO:PPLN (MgO-doped periodically poled lithium niobate), which is synchronously pumped by a 1040 nm femtosecond fiber laser. The OPO does not require any dielectric coating mirrors. By exploiting the four-prism sequence system, combined with the gold mirrors, the oscillating laser pulses could span the spectral regions in both the signal and idler, and the output pulses of OPO can be tuned across 1367-1914 nm in the signal, and 2152-4480 nm in the idler as well. This device can deliver as much as 1.2 W of average power at 1482 nm in the signal and up to 411 mW at 3487 nm in the idler, respectively. The ultrabroad-band spectra tunability, along with the high average output property, makes the dielectric-mirror-less OPO an attractive alternative to conventional OPOs.

Intensity noise suppression in mode-locked fiber lasers by double optical bandpass filtering.

We show that the relative intensity noise (RIN) of a mode-locked fiber laser can be suppressed below a -140 dB/Hz level for the entire >20 Hz offset frequency range by a proper combination of intra-cavity and extra-cavity optical bandpass filters. When a 12-nm-bandwidth intra-cavity filter and a 16-nm-bandwidth extra-cavity filter are employed for a polarization-maintaining-nonlinear-amplifying-loop-mirror (PM-NALM)-based Er-fiber laser, the RIN spectrum level is suppressed by ∼30 dB in the low offset frequency range. The resulting integrated rms RIN is only 0.0054% [1 Hz-1 MHz]-to our knowledge, one of the lowest integrated RIN performances for any mode-locked laser reported so far. Besides the simplicity, this double-filtering approach has an additional advantage: unlike active pump-laser feedback methods, it does not have any resonant peaks in the stabilized RIN spectrum. In addition to the RIN suppression, with intra-cavity bandpass filtering, the integrated rms timing jitter is also reduced from 7.29-fs (no-filter) to 2.95-fs (12-nm intra-cavity filter) [10 kHz-1 MHz] in the soliton PM-NALM laser.

Ultra-flat supercontinuum generated from high-power, picosecond telecommunication fiber laser source.

An ultra-flat, high-power supercontinuum generated from a picosecond telecommunication fiber laser was presented. The pulse from a carbon nanotube mode-locked oscillator was amplified using an Er-Yb codoped fiber amplifier. The output of the system achieved an average power of 2.7 W, with the center wavelength at 1564 nm and a FWHM of 6 nm in the spectral domain. By passing this amplified high-power pulse through a 4.6 m highly nonlinear photonic crystal fiber, an ultra-flat supercontinuum spanning 1600-2180 nm is generated. And the average power of the supercontinuum achieves 1 W.

A 3.7-kW Oscillating-Amplifying Integrated Fiber Laser Featuring a Compact Oval-Shaped Cylinder Package.

Combining the advantages of high efficiency, environmental robustness, and anti-reflection behavior, oscillating-amplifying integrated fiber lasers have become popular for use in high-power laser structures in industrial applications, wherein the size of the laser source matters. Here, an oscillating-amplifying integrated fiber laser in an oval-shaped cylinder package has been proposed and demonstrated, the footprint for which only occupies an area of 0.024 m2 apart from the pump diode, which is much smaller than in traditional planar fiber laser packages. Numerical simulations have been carried out, which have revealed that an oval-shaped cylinder package can effectively suppress the high-order mode in large mode area fiber setups and thereby benefit the integration of fusion points and the unpackaged elements at the same time. Over 3.7 kW of transverse mode instability (TMI)-free output power has been obtained, with a slope efficiency higher than 80%. With a custom-made chirped and tilted fiber Bragg grating (CTFBG), the Raman suppression ratio is improved to reach 38 dB at peak output power. The oval-shaped design has been verified to assist with the realization of TMI suppression and improve the integration of high-power fiber lasers. To the best of our knowledge, this fiber laser has among the smallest footprints of the various fiber sources at such high-power operating levels.

A Simple and Affordable Method to Create Nonsense Mutation Clones of p53 for Studying the Premature Termination Codon Readthrough Activity of PTC124.

(1) Background: A premature termination codon (PTC) can be induced by a type of point mutation known as a nonsense mutation, which occurs within the coding region. Approximately 3.8% of human cancer patients have nonsense mutations of p53. However, the non-aminoglycoside drug PTC124 has shown potential to promote PTC readthrough and rescue full-length proteins. The COSMIC database contains 201 types of p53 nonsense mutations in cancers. We built a simple and affordable method to create different nonsense mutation clones of p53 for the study of the PTC readthrough activity of PTC124. (2) Methods: A modified inverse PCR-based site-directed mutagenesis method was used to clone the four nonsense mutations of p53, including W91X, S94X, R306X, and R342X. Each clone was transfected into p53 null H1299 cells and then treated with 50 µM of PTC124. (3) Results: PTC124 induced p53 re-expression in H1299-R306X and H1299-R342X clones but not in H1299-W91X and H1299-S94X clones. (4) Conclusions: Our data showed that PTC124 more effectively rescued the C-terminal of p53 nonsense mutations than the N-terminal of p53 nonsense mutations. We introduced a fast and low-cost site-directed mutagenesis method to clone the different nonsense mutations of p53 for drug screening.

PTC124 Rescues Nonsense Mutation of Two Tumor Suppressor Genes NOTCH1 and FAT1 to Repress HNSCC Cell Proliferation.

(1) Background: PTC124 (Ataluren) is an investigational drug for the treatment of nonsense mutation-mediated genetic diseases. With the exception of the TP53 tumor suppressor gene, there has been little research on cancers with nonsense mutation. By conducting a database search, we found that another two tumor suppressor genes, NOTCH1 and FAT1, have a high nonsense mutation rate in head and neck squamous cell carcinoma (HNSCC). PTC124 may re-express the functional NOTCH1 or FAT1 in nonsense mutation NOTCH1 or FAT1 in HSNCC (2) Methods: DOK (with NOTCH1 Y550X) or HO-1-u-1 (with FAT1 E378X) HNSCC cells were treated with PTC124, and the NOTCH1 or FAT1 expression, cell viability, and NOTCH1- or FAT1-related downstream gene profiles were assayed. (3) Results: PTC124 was able to induce NOTCH1 or FAT1 expression in DOK and HO-1-u-1 cells. PTC124 was able to upregulate NOTCH downstream genes HES5, AJUBA, and ADAM10 in DOK cells. PTC124 enhanced DDIT4, which is under the control of the FAT1-YAP1 pathway, in HO-1-u-1 cells. FLI-06 (a NOTCH signaling inhibitor) reversed PTC124-mediated cell growth inhibition in DOK cells. PTC124 could reverse TT-10 (a YAP signaling activator)-mediated HO-1-u-1 cell proliferation. (4) Conclusions: PTC124 can rescue nonsense mutation of NOTCH1 and FAT1 to repress HNSCC cell proliferation.

P63 and P73 Activation in Cancers with p53 Mutation.

The members of the p53 family comprise p53, p63, and p73, and full-length isoforms of the p53 family have a tumor suppressor function. However, p53, but not p63 or p73, has a high mutation rate in cancers causing it to lose its tumor suppressor function. The top and second-most prevalent p53 mutations are missense and nonsense mutations, respectively. In this review, we discuss possible drug therapies for nonsense mutation and a missense mutation in p53. p63 and p73 activators may be able to replace mutant p53 and act as anti-cancer drugs. Herein, these p63 and p73 activators are summarized and how to improve these activator responses, particularly focusing on p53 gain-of-function mutants, is discussed.

Comparative evaluation of thermal decomposition behavior and thermal stability of powdered ammonium nitrate under different atmosphere conditions.

In order to analyze the thermal decomposition characteristics of ammonium nitrate (AN), its thermal behavior and stability under different conditions are studied, including different atmospheres, heating rates and gas flow rates. The evolved decomposition gases of AN in air and nitrogen are analyzed with a quadrupole mass spectrometer. Thermal stability of AN at different heating rates and gas flow rates are studied by differential scanning calorimetry, thermogravimetric analysis, paired comparison method and safety parameter evaluation. Experimental results show that the major evolved decomposition gases in air are H2O, NH3, N2O, NO, NO2 and HNO3, while in nitrogen, H2O, NH3, NO and HNO3 are major components. Compared with nitrogen atmosphere, lower initial and end temperatures, higher heat flux and broader reaction temperature range are obtained in air. Meanwhile, higher air gas flow rate tends to achieve lower reaction temperature and to reduce thermal stability of AN. Self-accelerating decomposition temperature of AN in air is much lower than that in nitrogen. It is considered that thermostability of AN is influenced by atmosphere, heating rate and gas flow rate, thus changes of boundary conditions will influence its thermostability, which is helpful to its safe production, storage, transportation and utilization.