Water Quality Assessment of Tributaries of Batang Baleh in Sarawak Using Cluster Analysis.

Assessment of river water quality is essential as it provides the knowledge required to make informed decisions. Therefore, water quality was determined at 15 tributary stations located along the Batang Baleh, Sarawak. Results of the study indicate that all tributaries were well-aerated (≈ 7.7 mg/L) with pH (≈ 7.3) and conductivity (≈ 37.3 µS/cm) values falling within acceptable ranges. However, there were tributaries that showed very high turbidity (> 1000 NTU) and suspended solids (> 800 mg/L) which were contributed by the soil erosion from logging activities in the watershed. Tributary stations associated with logging activities also showed significantly higher total phosphorus and organic nitrogen. Cluster analysis demonstrated that water quality at tributary stations along the Batang Baleh exhibited a longitudinal variation from upstream to downstream regions, particularly, dissolved oxygen, five-day biochemical oxygen demand, and nitrite-nitrate nitrogen, which were found higher in upstream region and steadily decreased towards the downstream region. Two stations located at Sg. Serani and Sg. Melatai were distinct from the other stations with the highest concentrations of turbidity, total suspended solids, organic nitrogen, and total phosphorus. Thus, there is an urgent need to reduce the pollutants in the tributaries of Batang Baleh for the health of the sensitive aquatic organisms.

Type and Quantity of Shipborne Garbage at Selected Tropical Beaches.

Marine debris is widely distributed at the coastal area of the global oceans; however, shipborne garbage source studies are still lacking to document the pollution in Malaysia Territorial Water. Thus, this study has adopted a standard method of beach marine debris survey at five beaches and inspected 115 vessels to assess the type and amount of debris from shipping source stranded on the beach. This study found that vessel visiting Malaysian ports observed the MARPOL 73/78 Annex V requirements; however, identified objects from shipping activity (1.3%; 2 items/km) found on the beaches indicate that there are vessels disposing of garbage illegally at sea. Therefore, there is a need to promote the use of biodegradable material and introduce environmental education to increase awareness on the vessel.

Baseline trace metals in water and sediment of the Baleh River-a tropical river in Sarawak, Malaysia.

Quantitative indices are classically employed to evaluate the contamination status of metals with reference to the baseline concentrations. The baselines vary considerably across different geographical zones. It is imperative to determine the local geochemical baseline to evaluate the contamination status. No study has been done to establish the background concentrations in tropical rivers of this region. This paper reports the background concentrations of metals in water and sediment of the Baleh River, Sarawak, derived based on the statistical methods where the areas possibly disturbed are distinguished from the undisturbed area. The baseline levels of six elements in water determined were Al (0.34 mg/L), Fe (0.51 mg/L), Mn (0.12 mg/L), Cu (0.01 mg/L), Pb (0.03 mg/L), and Zn (0.05 mg/L). Arsenic and selenium were below the detection limit. For sediment, the background values were established according to statistical methods including (mean + 2σ), iterative 2σ, cumulative distribution frequency, interquartile, and calculation distribution function. The background values derived using the iterative 2σ algorithm and calculated distribution function were relatively lower. The baseline levels calculated were within the range reported in the literatures mainly from tropical and sub-tropical regions. The upper limits proposed for nine elements in sediment were Al (100,879 mg/kg), Cr (75.45 mg/kg), Cu (34.59 mg/kg), Fe (37,823 mg/kg), Mn (793 mg/kg), Ni (22.88 mg/kg), Pb (27.26 mg/kg), Zn (70.64 mg/kg), and Hg (0.33 mg/kg). Quantitative indices calculated suggest low risk of contamination at the Baleh River.

A novel computer-aided multivariate water quality index.

A computer-aided multivariate water quality index is developed based on partial least squares (PLS) regression. The index is termed as the partial least squares water quality index (PLS-WQI). Briefly, a training set was computationally generated based on the guideline of National Water Quality Standards for Malaysia (NWQS) to predict the water quality. The index is benchmarked with the well-established index developed by the Department of Environment, Malaysia (DOE-WQI). The PLS-WQI is a continuous variable with the value closer to I indicating good water quality and closer to V indicating poor water quality. Unlike other conventional indexing methods, the algorithm calculates the index in a multivariate manner. The algorithm allows rapid processing of a large dataset without tedious calculation; it can be an efficient tool for spatial and temporal routine monitoring of water quality. Although the algorithm is designed based on the guideline of NWQS, it can be easily adapted to accommodate other guidelines. The algorithm was evaluated and demonstrated on the simulated and real datasets. Results indicate that the algorithm is robust and reliable. Based on six parameters, the overall ratings derived are inversely correlated to DOE-WQI. When the number of parameter is increased, the overall ratings appear to provide better insights into the water quality.

Expression trend of selected ribosomal protein genes in nasopharyngeal carcinoma.

BACKGROUND: Ribosomal proteins are traditionally associated with protein biosynthesis until recent studies that implicated their extraribosomal functions in human diseases and cancers. Our previous studies using GeneFishing™ DEG method and microarray revealed underexpression of three ribosomal protein genes, RPS26, RPS27, and RPL32 in cancer of the nasopharynx. Herein, we investigated the expression pattern and nucleotide sequence integrity of these genes in nasopharyngeal carcinoma to further delineate their involvement in tumourigenesis. The relationship of expression level with clinicopathologic factors was also statistically studied. METHODS: Quantitative Polymerase Chain Reaction was performed on nasopharyngeal carcinoma and their paired normal tissues. Expression and sequence of these three genes were analysed. RESULTS: All three ribosomal protein genes showed no significant difference in transcript expressions and no association could be established with clinicopathologic factors studied. No nucleotide aberrancy was detected in the coding regions of these genes. CONCLUSION: There is no early evidence to substantiate possible involvement of RPS26, RPS27, and RPL32 genes in NPC tumourigenesis.

Fish biodiversity and assemblages along the altitudinal gradients of tropical mountainous forest streams.

Knowledge of the fundamental aspects of ecology such as the patterns of fish species distribution and biodiversity in the forest streams is the first and basic step to develop effective conservation strategies. Yet, studies on altitudinal changes of fish composition and assemblages in Bornean forest streams are scarce despite being one of the hotspots of biodiversity conservation. Hence, surveys on freshwater fish composition along the altitudinal gradients of the Baleh River Basin in Sarawak, Borneo were conducted from April 2014 to August 2015. The Baleh River Basin was divided into seven altitudinal groups with a total of 72 stations. Group elevation ranged from 53 to 269 m above sea level. The fish samples and environmental parameters were taken concurrently during samplings. A total of 3565 specimens belonging to six orders, 14 families, and 76 species were found in the present study. The most dominant family in the Baleh River Basin was Cyprinidae (74.4%), followed by Gastromyzontidae (16.2%) while the most dominant species was Tor tambra (12.9%), followed by Lobocheilos ovalis (12.3%). Fish abundance significantly higher at high altitude sites than those at low altitude sites except for Mengiong River which has the lowest fish abundance despite with high elevation. Species richness was found significantly lower in midstream segment. Noticeable altitudinal gradient of fish assemblages was observed along the Baleh River except a discontinuity at the midstream segment which is attributable to the poorer quality inflow from the Mengiong River coupled with the meandering feature of the segment. Fish abundance was significantly and positively correlated with elevation, water pH and conductivity while negatively correlated with turbidity. Anthropogenic activities in the Baleh River Basin had altered the environmental variables thus disrupted the altitudinal gradient of fish assemblages. This phenomenon is apparent when the Canonical Correspondence Analysis (CCA) revealed that the first axis (CCA1) explained 42.5% of the variation and has positive loading on dissolved oxygen (DO) and negative loading on water conductivity; whereas CCA2 explained 37.5% of the variation and positively loaded on elevation, water pH, and DO. The results demonstrated that Gastromyzon fasciatus preferred more oxygenated water than Protomyzon sp., G. sp 1, and G. punctulatus although they are all from Gastromyzontidae family that inhabiting high altitude sites. Barbonymus schwanenfeldii was also found most abundant with elevated dissolved oxygen value. On the other hand, Rasbora volzii and R. hosii inhabiting lower altitude sites with less oxygenated and more acidic water.

Distribution of Major and Trace Elements in a Tropical Hydroelectric Reservoir in Sarawak, Malaysia.

This paper reports the metals content in water, sediment, macroalgae, aquatic plant, and fish of Batang Ai Hydroelectric Reservoir in Sarawak, Malaysia. The samples were acid digested and subjected to atomic absorption spectrometry analysis for Na, K, Mn, Cr, Ni, Zn, Mg, Fe, Sn, Al, Ca, As, Se, and Hg. The total Hg content was analysed on the mercury analyser. Results showed that metals in water, sediment, macroalgae, aquatic plant, and fish are distinguishable, with sediment and biota samples more susceptible to metal accumulation. The distributions of heavy metals in water specifically Se, Sn, and As could have associated with the input of fish feed, boating, and construction activities. The accumulation of heavy metals in sediment, macroalgae, and aquatic plant on the other hand might be largely influenced by the redox conditions in the aquatic environment. According to the contamination factor and the geoaccumulation index, sediment in Batang Ai Reservoir possesses low risk of contamination. The average metal contents in sediment and river water are consistently lower than the literature values reported and well below the limit of various guidelines. For fishes, trace element Hg was detected; however, the concentration was below the permissible level suggested by the Food and Agriculture Organization.