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1.
J Asian Nat Prod Res ; 26(8): 918-929, 2024 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-38629733

RESUMEN

Bicyclol is a hepatoprotective agent widely used for treating chronic hepatitis and drug-induced liver injuries in clinics. The purpose of the study was to elucidate the contribution of CYP450 enzymes to the metabolism of bicyclol using the relative activity factor approach. After incubation with human liver microsomes and recombinant human liver CYP450 enzymes, the calculated contribution of CYP3A4 and 2C19 to the metabolism of bicyclol was 85.6-90.3% and 9.2-9.7%, respectively. The metabolism was interrupted in the presence of CYP3A4 and 2C19 selective inhibitors. These findings help to predict or avoid metabolic drug-drug interactions or toxicity in clinical applications of bicyclol.


Asunto(s)
Compuestos de Bifenilo , Sistema Enzimático del Citocromo P-450 , Microsomas Hepáticos , Humanos , Microsomas Hepáticos/metabolismo , Compuestos de Bifenilo/farmacología , Estructura Molecular , Sistema Enzimático del Citocromo P-450/metabolismo , Citocromo P-450 CYP3A/metabolismo
2.
Clin Oral Investig ; 27(8): 4493-4501, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-37219608

RESUMEN

OBJECTIVES: The aim of this study was to evaluate the clinical outcomes of diode laser as an adjunct to nonsurgical periodontal therapy (NSPT) for residual periodontal pockets in mandibular second molars. MATERIALS AND METHODS: Sixty-seven mandibular second molars (154 residual periodontal pockets) were recruited into the study and randomly assigned to the Laser + NSPT group and the NSPT group. The Laser + NSPT group underwent NSPT adjunct with diode laser radiation (wavelength: 810 nm, power: 1.5 W, 40 s maximum), while the NSPT group underwent nonsurgical periodontal therapy alone. Clinical parameters were measured at baseline (T0) and 4(T1), 12(T2), and 24(T3), weeks after treatment. RESULTS: Periodontal pocket depth (PPD), clinical attachment loss (CAL), and bleeding on probing (BOP) in both groups showed significant improvements at the end of study compared to baseline. The reductions of PPD, CAL, and BOP in the Laser + NSPT group were significantly greater than NSPT group. At T3, the Laser + NSPT group had a mean PPD of 3.06 ± 0.86 mm, CAL of 2.58 ± 0.94 mm and BOP of 15.49%, while the NSPT group had a mean PPD of 4.46 ± 1.57 mm, CAL of 3.03 ± 1.25 mm and BOP of 64.29%. CONCLUSIONS: The diode laser as an adjunct to nonsurgical periodontal therapy may contribute to clinical outcomes for residual periodontal pockets. However, the approach may cause reduction of keratinized tissue width. TRIAL REGISTRATION NUMBER: This study was registered in the Chinese Clinical Trial Registry ChiCTR2200061194. CLINICAL RELEVANCE: Diode laser as an adjunct to nonsurgical periodontal therapy may contribute to the clinical outcomes for residual periodontal pockets in mandibular second molars.


Asunto(s)
Periodontitis Crónica , Terapia por Láser , Terapia por Luz de Baja Intensidad , Humanos , Periodontitis Crónica/radioterapia , Bolsa Periodontal/radioterapia , Láseres de Semiconductores/uso terapéutico , Raspado Dental
3.
Oral Dis ; 25(7): 1789-1797, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31283861

RESUMEN

BACKGROUND: Porphyromonas gingivalis is the main pathogen of periodontal disease affecting over half of the worldwide adult population. Recent studies have shown that P. gingivalis is related to the development of non-alcoholic fatty liver disease (NAFLD), a global major chronic liver disease, especially in developed countries. However, how P. gingivalis contributes to the pathogenesis of NAFLD has not been fully clarified. We aimed to conduct a preliminary exploration of the underlying mechanism of P. gingivalis infection in the development of NAFLD. METHODS: Human hepatocellular cells HepG2 were incubated with/without oleic acid (OA) and tested for lipid accumulation upon stimulation by lipopolysaccharide (LPS) derived from P. gingivalis or Escherichia coli. Intracellular lipid droplet formation was analyzed and quantified by Oil Red O staining. The involvement of signaling pathway molecules and pro-inflammatory cytokines related to NF-κB and MAPKs were examined with Western blot and quantitative real-time PCR (qRT-PCR) analyses and further evaluated with inhibitor treatment and RNA interference. RESULTS: HepG2 cells accumulated more intracellular lipids when stimulated with P. gingivalis LPS, as compared to cells treated with E. coli LPS or control. Further pathway analysis demonstrated that after stimulation with P. gingivalis LPS, cells displayed significantly upregulated MyD88 expression, increased phosphorylation of p65 and JNK, and more release of pro-inflammatory cytokines, such as IL-1, IL-8, and TNF-α. In addition, suppression of phosphorylation of p65 and JNK by inhibitors and RNA interference resulted in a reduction in lipid accumulation upon P. gingivalis LPS treatment. CONCLUSIONS: These results suggest that P. gingivalis-derived LPS may contribute to intracellular lipid accumulation and inflammatory reaction of HepG2 cells via the activation of NF-κB and JNK signaling pathways. This study offers a possible explanation to the functional involvement of P. gingivalis infection in the pathological progression of NAFLD. These findings may help design new treatment strategies in NAFLD.


Asunto(s)
Lipopolisacáridos , Sistema de Señalización de MAP Quinasas , FN-kappa B , Enfermedad del Hígado Graso no Alcohólico/patología , Periodontitis/microbiología , Porphyromonas gingivalis , Adulto , Infecciones por Bacteroidaceae , Western Blotting , Humanos , Enfermedad del Hígado Graso no Alcohólico/microbiología , Porphyromonas gingivalis/aislamiento & purificación , Reacción en Cadena en Tiempo Real de la Polimerasa
4.
Yi Chuan ; 38(6): 532-542, 2016 06 20.
Artículo en Inglés | MEDLINE | ID: mdl-27655315

RESUMEN

Rice flowering regulation is an extremely complex process, which is controlled by genetic factors and external environment. Photoperiodic regulatory pathway is pivotal to control flowering in rice, in which florigen genes Hd3a and RTF1 are at the core and they are regulated by upstream Hd1-dependent, Ehd1-dependent, as well as both Hd1- and Ehd1-independent pathways. The three pathways bring a variety of light signal information together to Hd3a and RTF1 for further integration, and then transmit the signals in the form of florigen to the downstream flowering related genes. In this review, we summarize the research progress of photoperiod regulated genes on flowering time in rice, including the photoreceptors and circadian rhythm genes, the florigens, its upstream, downstream and interacting genes. We hope to provide a reference for in-depth study of rice flowering regulation.


Asunto(s)
Flores/genética , Genes Reguladores/genética , Oryza/genética , Regulación de la Expresión Génica de las Plantas/genética , Genes de Plantas/genética , Fotoperiodo , Proteínas de Plantas/genética
5.
PLoS Pathog ; 9(8): e1003545, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23990780

RESUMEN

Nuclear hormone receptors respond to small molecules such as retinoids or steroids and regulate development. Signaling in the conserved p38/PMK-1 MAP kinase pathway regulates innate immunity. In this study, we show that the Caenorhabditis elegans nuclear receptor DAF-12 negatively regulates the defense against pathogens via the downstream let-7 family of microRNAs, which directly target SKN-1, a gene downstream of PMK-1. These findings identify nuclear hormone receptors as components of innate immunity that crosstalk with the p38/PMK-1 MAP kinase pathway.


Asunto(s)
Proteínas de Caenorhabditis elegans/inmunología , Caenorhabditis elegans/inmunología , Inmunidad Innata/fisiología , Sistema de Señalización de MAP Quinasas/inmunología , MicroARNs/inmunología , Receptores Citoplasmáticos y Nucleares/inmunología , Animales , Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/genética , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/inmunología , Sistema de Señalización de MAP Quinasas/genética , MicroARNs/genética , Proteínas Quinasas Activadas por Mitógenos/genética , Proteínas Quinasas Activadas por Mitógenos/inmunología , Receptores Citoplasmáticos y Nucleares/genética , Factores de Transcripción/genética , Factores de Transcripción/inmunología
6.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 35(12): 1463-8, 2015 Dec.
Artículo en Zh | MEDLINE | ID: mdl-26882609

RESUMEN

OBJECTIVE: To observe the effects of acupuncture on neurofunction and neuropsychological factors of chronic alcoholic peripheral neuropathy (CAPN) patients. METHODS: Totally 120 CAPN patients were assigned to the common treatment group, acupuncture group A, and acupuncture group B according to random digit table, 40 in each group. All patients recieved conventional drug therapy. Besides, patients in the acupuncture group A were additionally needled at Pishu (BL20), Weishu (BL21), Xuehai (SP10), Yinlingquan (SP9), Zusanli (ST36), Yanglingquan (GB34), Jiexi (ST41), Xuanzhong (GB39), Xiangu (ST43),Taixi (KI3), Quchi (LI11), Waiguan (SJ5), Hegu (LI4), and so on. On these bases patients in the acupuncture group B were needled at Sishencong (EX-HN1), Yintang (EX-HN3), Neiguan (PC6), Taichong (LR3), Sanyinjiao (SP6), and Taiyang (EX-HN5). Acupuncture was performed once a day, 14 times as a course; and then once on every other day, 14 times in total for 4 weeks. All treatment lasted for 8 successive weeks. Neuropathy Impairment Score in the Lower Limbs (NIS-LL), Neurological Severity Score (NSS), Hamilton Depression Scale (HAMD), and Hamilton Anxiety Scale (HAMA) were assessed, motor nerve conduction velocity (MCV) and sensory nerve conduction velocity (SCV) were detected before and after treatment. RESULTS: After 8 weeks of treatment the scores of NIS-LL and NSS significantly decreased in the 3 groups, with statistical difference as compared with before treatment (P < 0.05). Scores of NIS-LL and NSS decreased more in acupuncture groups A and B than in the common treatment group (P < 0.05), and more obvious in acupuncture group B (P < 0.05). Compared with the same group before treatment, MCV and SCV of median nerve, ulnar nerve, common peroneal nerve and tibial nerve increased in acupuncture treatment group A and B after 8-week treatment (P < 0.05). MCV of median nerve, MCV and SCV of common peroneal nerve and tibial nerve significantly increased in the common treatment group (P < 0.05). Compared with the common treatment group, SCV of median nerve, MCV and SCV of ulnar nerve, common peroneal nerve and tibial nerve obviously increased in acupuncture treatment groups A and B after treatment (P < 0.05). MCV of ulnar nerve, MCV and SCV of common peroneal nerve and tibial nerve obviously increased more in acupuncture treatment group A than in acupuncture treatment group B (P < 0.05). At week 8 after treatment scores of HAMD and HAMA were obviously lowered in acupuncture groups A and B, with statistical difference as compared with before treatment (P < 0.05). The scores of HAMD were also decreased in the common treatment group, as compared with before treatment (P < 0.05). At week 8 after treatment scores of HAMD and HAMA were obviously lowered more in acupuncture treatment group B than in acupuncture treatment group A (P < 0.05). CONCLUSION: Acupuncture therapy could effectively improve the neurofunction of CAPN patients, and improve complicated anxiety and depression by additionally needling at Sishencong (EX-HN1), Yintang (EX-HN3), Taichong (LR3), Sanyinjiao (SP6), and Taiyang (EX-HN5).


Asunto(s)
Terapia por Acupuntura , Trastornos Relacionados con Alcohol/terapia , Enfermedades del Sistema Nervioso Periférico/terapia , Puntos de Acupuntura , Ansiedad , Depresión , Trastorno Depresivo , Medicamentos Herbarios Chinos , Humanos
7.
Mol Oral Microbiol ; 38(4): 309-320, 2023 08.
Artículo en Inglés | MEDLINE | ID: mdl-37216657

RESUMEN

INTRODUCTION: MicroRNAs (miRNAs), a type of non-coding RNA, have been demonstrated to be essential posttranscriptional modulators in oral diseases and inflammatory responses. However, the specific role of miR-27a-5p in periodontitis requires further investigation. In this study, we used both cellular and animal models to determine how miR-27a-5p affects the pathogenesis of periodontitis and its associated biological functions. METHODS: Quantitative real-time polymerase chain reaction and western blotting were used to analyze the expression of cytokines, phosphatase and tensin homolog deleted on chromosome ten (PTEN), and miR-27a-5p transcription. Investigation of alveolar bone resorption and inflammation of the periodontium in ligature-induced periodontitis in mice was performed using micro-computed tomography (micro-CT), hematoxylin-eosin (HE) staining, and tartrate-resistant acid phosphatase (TRAP) staining. The binding of miR-27a-5p and PTEN was predicted using the TargetScan database and experimentally confirmed using dual luciferase reporter gene assays. RESULTS: The inflamed gingiva showed lower levels of miR-27a-5p. Macrophages from miR-27a-5p-/- mice produced much higher quantities of pro-inflammatory cytokines owing to the stimulation of Porphyromonas gingivalis lipopolysaccharide, and miR-27a-5p-/- mice with ligature-induced periodontitis also exhibited more severe alveolar bone resorption and damage to the periodontium. Target validation assays identified PTEN as a direct target of bona. Blocking PTEN expression partially reduced inflammation, both in vitro and in vivo. CONCLUSIONS: miR-27a-5p alleviated the inflammatory response in periodontitis by targeting PTEN.


Asunto(s)
Resorción Ósea , MicroARNs , Periodontitis , Ratones , Animales , Tensinas/genética , Microtomografía por Rayos X , MicroARNs/genética , MicroARNs/metabolismo , Inflamación , Fosfohidrolasa PTEN/genética , Fosfohidrolasa PTEN/metabolismo , Citocinas/genética , Periodontitis/genética , Cromosomas/metabolismo , Resorción Ósea/genética
8.
Arch Biochem Biophys ; 522(1): 9-16, 2012 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-22521737

RESUMEN

Periodontal ligament (PDL) cells are fibroblasts that play key roles in tissue integrity, periodontal inflammation and tissue regeneration in the periodontium. The periodontal tissue destruction in periodontitis is mediated by host tissue-produced inflammatory cytokines, including interleukin-1ß (IL-1ß). Here, we report the expression of G protein-coupled receptor 30 (GPR30, also known as G protein-coupled estrogen receptor 1 GPER) in human PDL cells and its regulation by IL-1ß. IL-1ß-induced GPR30 expression in human PDL cells leads to the activation of multiple signaling pathways, including MAPK, NF-κB and PI3K. In contrast, genistein, an estrogen receptor ligand, postpones the activation of MAPKs induced by IL-1ß. Moreover, the inhibition of GPR30 by G15, a GPR30-specific antagonist, eliminates this delay. Thus, genistein plays a role in the regulation of MAPK activation via GPR30, and GPR30 represents a novel target regulated by steroid hormones in PDL cells.


Asunto(s)
Genisteína/farmacología , Interleucina-1beta/fisiología , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Ligamento Periodontal/efectos de los fármacos , Receptores de Estrógenos/fisiología , Receptores Acoplados a Proteínas G/fisiología , Adolescente , Secuencia de Bases , Células Cultivadas , Cartilla de ADN , Activación Enzimática , Femenino , Humanos , Masculino , Ligamento Periodontal/citología , Ligamento Periodontal/enzimología , Reacción en Cadena en Tiempo Real de la Polimerasa , Receptores de Estrógenos/antagonistas & inhibidores , Receptores Acoplados a Proteínas G/antagonistas & inhibidores
9.
J Oral Sci ; 64(3): 212-217, 2022 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-35644563

RESUMEN

PURPOSE: This study investigated and tested a novel cone-beam computed tomography (CBCT) scanning technique capable of obtaining clear contours of soft tissues in the esthetic area. METHODS: Twenty-three periodontally healthy participants underwent this novel CBCT scanning technique. Soft tissue morphological parameters were measured on the CBCT images obtained. Intraoral clinical data were also collected at the same locations, and the accuracy of the CBCT method was tested. RESULTS: The median (interquartile range [IQR]) of the supracrestal gingival tissue thickness as 0.91 (0.73-1.13) mm, and the thickness of the central incisors was significantly greater than that of the canines (P < 0.05). The median (IQR) of keratinized tissue thickness was 0.73 (0.55-0.91) mm, which also showed a significantly greater thickness in the central incisors than in the canines (P < 0.05). Bland-Altman analysis suggested that CBCT could be accurate for measuring soft tissues in the esthetic area. CONCLUSION: The novel CBCT technique described yields clear contours of soft tissues in the esthetic area without the need for auxiliary tools. Moreover, measurements of soft tissue morphological parameters on CBCT appear to be accurate.


Asunto(s)
Tomografía Computarizada de Haz Cónico , Diente , Tomografía Computarizada de Haz Cónico/métodos , Estética , Encía/diagnóstico por imagen , Humanos
10.
J Integr Plant Biol ; 52(11): 981-95, 2010 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-20977656

RESUMEN

Following the idea of partial root-zone drying (PRD) in crop cultivation, the morphological and physiological responses to partial root osmotic stress (PROS) and whole root osmotic stress (WROS) were investigated in rice. WROS caused stress symptoms like leaf rolling and membrane leakage. PROS stimulated stress signals, but did not cause severe leaf damage. By proteomic analysis, a total of 58 proteins showed differential expression after one or both treatments, and functional classification of these proteins suggests that stress signals regulate photosynthesis, carbohydrate and energy metabolism. Two other proteins (anthranilate synthase and submergence-induced nickel-binding protein) were upregulated only in the PROS plants, indicating their important roles in stress resistance. Additionally, more enzymes were involved in stress defense, redox homeostasis, lignin and ethylene synthesis in WROS leaves, suggesting a more comprehensive regulatory mechanism induced by osmotic stress. This study provides new insights into the complex molecular networks within plant leaves involved in the adaptation to osmotic stress and stress signals.


Asunto(s)
Oryza/metabolismo , Ósmosis/fisiología , Hojas de la Planta/metabolismo , Proteómica/métodos , Transducción de Señal , Estrés Fisiológico , Secuencia de Aminoácidos , Electroforesis en Gel Bidimensional , Espectrometría de Masas , Datos de Secuencia Molecular , Oryza/anatomía & histología , Proteínas de Plantas/química , Proteínas de Plantas/clasificación , Proteínas de Plantas/metabolismo , Raíces de Plantas/fisiología
11.
J Integr Plant Biol ; 51(9): 879-88, 2009 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-19723247

RESUMEN

In order to explore the relevant molecular genetic mechanisms of photosynthetic rate (PR) and chlorophyll content (CC) in rice (Oryza sativa L.), we conducted a series of related experiments using a population of recombinant inbred lines (Zhenshan97B x IRAT109). We found a significant correlation between CC and PR (R= 0.19**) in well-watered conditions, but no significant correlation during water stress (r= 0.08). We detected 13 main quantitative trait loci (QTLs) located on chromosomes 1, 2, 3, 4, 5, 6, and 10, which were associated with CC, including six QTLs located on chromosomes 1, 2, 3, 4, and 5 during water stress, and seven QTLs located on chromosomes 2, 3, 4, 6, and 10 in well-watered conditions. These QTLs explained 47.39% of phenotypic variation during water stress and 56.19% in well-watered conditions. We detected four main QTLs associated with PR; three of them (qPR2, qPR10, qPR11) were located on chromosomes 2, 10, and 11 during water stress, and one (qPR10) was located on chromosome 10 in well-watered conditions. These QTLs explained 34.37% and 18.41% of the phenotypic variation in water stress and well-watered conditions, respectively. In total, CC was largely controlled by main QTLs, and PR was mainly controlled by epistatic QTL pairs.


Asunto(s)
Clorofila/metabolismo , Oryza/genética , Oryza/metabolismo , Fotosíntesis/fisiología , Sitios de Carácter Cuantitativo/genética , Deshidratación , Regulación de la Expresión Génica de las Plantas/genética , Regulación de la Expresión Génica de las Plantas/fisiología , Oryza/fisiología , Fotosíntesis/genética
13.
Yi Chuan Xue Bao ; 33(9): 824-32, 2006 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-16980129

RESUMEN

Photosynthesis of carbohydrate is the primary source of grain yield in rice (Oryza sativa L.). It is important to genetically analyze the morphological and the physiological characteristics of functional leaves, especially flag leaf, in rice improvement. In this study, a recombinant inbred population derived from a cross between an indica (O. sativa L. ssp. indica) cultivar and a japonica (O. sativa L. ssp. japonica) cultivar was employed to map quantitative traits loci (QTLs) for the morphological (i.e., leaf length, width, and area) and physiological (i.e., leaf color rating and stay-green) characteristics of flag leaf and their relationships with yield and yield traits in 2003 and 2004. A total of 17 QTLs for morphological traits (flag leaf length, width, and area), 6 QTLs for degree of greenness and 14 QTLs for stay-green-related traits (retention-degrees of greenness, relative retention of greenness, and retention of the green area) were resolved, and 10 QTLs were commonly detected in both the years. Correlation analysis revealed that flag leaf area increased grain yield by increasing spikelet number per panicle. However, the physiological traits including degree of greenness and stay-green traits were not or negatively correlated to grain yield and yield traits, which may arise from the negative relation between degree of greenness and flag leaf size and the partial sterility occurred in a fraction of the lines in this population. The region RM255-RM349 on chromosome 4 controlled the three leaf morphological traits simultaneously and explained a large part of variation, which was very useful for genetic improvement of grain yield. The region RM422-RM565 on chromosome 3 was associated with the three stay-green traits simultaneously, and the use of this region in genetic improvement of grain yield needs to be assessed by constructing near-isogenic lines.


Asunto(s)
Grano Comestible/genética , Epistasis Genética , Genes de Plantas/fisiología , Oryza/genética , Hojas de la Planta/genética , Sitios de Carácter Cuantitativo/genética , Mapeo Cromosómico , Cromosomas de las Plantas , Productos Agrícolas/genética , Fenotipo , Fotosíntesis
14.
Yi Chuan Xue Bao ; 33(6): 532-41, 2006 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-16800384

RESUMEN

Abscisic acid (ABA) is one of the important plant hormones, which plays a critical role in seed development and adaptation to abiotic stresses. The sensitivity of rice (Oryza sativa L.) to exogenous ABA at seed germination and seedling stages was investigated in the recombinant inbred line (RIL) population derived from a cross between irrigated rice Zhenshan 97 and upland rice IRAT109, using relative germination vigor (RGV), relative germination rate (RGR) and leaf rolling scores of spraying (LRS) or culturing (LRC) with ABA as sensitivity indexes. The phenotypic correlation analysis revealed that only RGV at germination stage was positively correlated to ABA sensitivity at seedling stage. QTL detection using composite interval mapping (CIM) and mixed linear model was conducted to dissect the genetic basis of ABA sensitivity, and the single-locus QTLs detected by both methods are in good agreement with each other. Five single QTLs and six pairs of epistatic QTLs were detected for ABA sensitivity at germination stage. Eight single QTLs and five pairs of epistatic QTLs were detected for ABA sensitivity at seedling stage. Two QTLs were common between LRS and LRC; and one common QTL was detected for RGV, LRS and LRC simultaneously. These results indicated that both single and epistatic loci were involved in the ABA sensitivity in rice, and the genetic basis of ABA sensitivity at seed germination and seedling stage was largely different.


Asunto(s)
Ácido Abscísico/farmacología , Germinación/efectos de los fármacos , Germinación/genética , Oryza/genética , Sitios de Carácter Cuantitativo , Plantones/genética , Cromosomas de las Plantas , Genotipo , Oryza/efectos de los fármacos , Fenotipo , Carácter Cuantitativo Heredable , Plantones/efectos de los fármacos , Semillas/efectos de los fármacos , Sensibilidad y Especificidad
15.
Yi Chuan Xue Bao ; 33(5): 458-67, 2006 May.
Artículo en Inglés | MEDLINE | ID: mdl-16722341

RESUMEN

QTL underlying related traits at the late developmental stage under two different nitrogen levels were investigated in rice using a population of chromosome segment substitution lines (CSSL) derived from a cross between Teqing and Lemont. A total of 31 QTLs referring 5 traits, that is, plant height (PH), panicle number per plant (PN), chlorophyll content (CC), shoot dry weight (SDW) and grain yield per plant (YD), were detected. Under normal N level, 3 QTLs were detected for each trait, while under low N level, 5, 4, 5 and 2 QTLs were detected for PH, PN, CC and SDW respectively. Most of the QTLs were located on chromosome 2, 3, 7, 11 and 12. QTLs controlling different traits or the same trait under different N levels were mapped on the same or adjacent intervals, forming several clusters in rice chromosomes. More than two traits were controlled by QTLs on one of four intervals (RM30-RM439, RM18-RM478, RM309-RM270, and RM235-RM17), suggesting that there were some pleiotropic effects. It was supposed that some QTLs only detected at low N level might be associated with the ability to tolerate the low N stress in rice.


Asunto(s)
Clorofila/metabolismo , Cromosomas de las Plantas/efectos de los fármacos , Nitrógeno/farmacología , Oryza/genética , Sitios de Carácter Cuantitativo/genética , Mapeo Cromosómico , Oryza/crecimiento & desarrollo , Sitios de Carácter Cuantitativo/fisiología
16.
Yi Chuan Xue Bao ; 33(6): 542-50, 2006 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-16800385

RESUMEN

Four flowering related traits, spikelet number per panicle (SNP), percentage of single exserted stigma (PSES), dual exserted stigma (PDES) and total exserted stigma (PES) of a RI population with 185 lines under water stress and non-stress conditions for 2 years, were investigated in a drought tolerance screening facility. ANOVA results showed high significance between years, lines, and water stress treatments, together with interactions among them in pairs. Highest phenotypic correlation was found between PSES and PES (r = 0.9752***), followed by PDES and PES (r = 0.7150***), and PSES and PDES (r = 0.5424***). Based on a linkage map of 203 SSR markers, six main effect QTLs were detected for SNP and three or four main effect QTLs were associated with PSES, PDES and PES under stress or non-stress conditions. There were one to nine pairs of epistatic QTLs influencing SNP and stigma exsertion. The contribution rates of additive and epistatic effects seemed to be in a low magnitude for most cases (0.76%-9.92%) while a few QTLs or QTL pairs explained more than 10% of total variance. Some main effect QTL and epistasis were commonly detected among PSES, PDES and PES, explaining the high positive correlation between them. Few QTLs were detected under both water stress and non-stress condition, implying that drought had severe impact on the genetic behaviors of both spikelet number and stigma exsertion.


Asunto(s)
Deshidratación/genética , Epistasis Genética , Flores/genética , Oryza/genética , Sitios de Carácter Cuantitativo , Productos Agrícolas/genética , Oryza/fisiología , Semillas
17.
Int J Mol Med ; 38(1): 131-8, 2016 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-27246196

RESUMEN

MicroRNAs (miRNAs or miRs) are small, non-coding RNA molecules that play significant roles in numerous diseases. However, there is limited information regarding the plasma expression of miRNAs in patients with primary biliary cirrhosis (PBC) as well as the potential role of miRNAs in the development of PBC. miRNA microarray analysis was performed using plasma obtaind from three patients with PBC and three healthy controls. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to confirm the differential expression of miRNAs in the plasma and peripheral blood mononuclear cells (PBMCs) isolated from 20 patients with PBC, 20 patients with chronic hepatitis B (CHB) and 20 healthy controls. These miRNAs in PBMCs and plasma were validated by linear regression analyses. The T cell subset frequency was analyzed by flow cytometry. Correlations between altered miRNA expression and the frequency of the T cell subsets were determined by linear regression analyses. The co-expression of miRNAs and IL-17A was examined using fluorescence in situ hybridization (FISH) and immunohistochemistry. The microarray analysis identified sixteen miRNAs that were differentially expressed. Four miRNAs were validated by RT-qPCR. The expression pattern of miR-572 and miR-92a in the PBMCs correlated with the expression pattern in plasma. We also found that miR-92a expression closely correlated with the frequency of a subset of IL-17-producing T helper cells (Th17), and that miR-92a was co-expressed with IL-17A in patients with PBC. Taken together, these findings revealed that plasma from patients with PBC has a unique miRNA expression profile. Moreover, miR-92a may play an important role in the pathogenesis of PBC by regulating Th17 cell differentiation.


Asunto(s)
Regulación de la Expresión Génica , Cirrosis Hepática Biliar/genética , Cirrosis Hepática Biliar/inmunología , MicroARNs/genética , Células Th17/metabolismo , Adulto , Femenino , Citometría de Flujo , Perfilación de la Expresión Génica , Humanos , Interleucina-17/metabolismo , Cirrosis Hepática Biliar/sangre , Recuento de Linfocitos , Subgrupos Linfocitarios/inmunología , Masculino , MicroARNs/metabolismo , Análisis de Secuencia por Matrices de Oligonucleótidos , Reacción en Cadena en Tiempo Real de la Polimerasa , Reproducibilidad de los Resultados
18.
Cell Signal ; 28(9): 1292-1303, 2016 09.
Artículo en Inglés | MEDLINE | ID: mdl-27234131

RESUMEN

CD36, a scavenger receptor, plays an important role in the progression of atherosclerosis through its interaction with oxidized low-density lipoprotein (ox-LDL). Porphyromonas gingivalis (P. gingivalis, Pg) has been shown to promote macrophage-derived foam cell formation by affecting the expression of CD36. However, the regulatory role of CD36 in macrophages infected with Pg remains largely unknown. Therefore, the aim of the present study was to explore the molecular mechanism of Pg induced CD36 expression in macrophages. Our results showed that Pg promoted ox-LDL uptake by macrophages and the formation of foam cells. Pg infection increased CD36 mRNA and protein levels in ox-LDL-untreated macrophages. Moreover, small interferon RNA (siRNA) targeting CD36 significantly reduced foam cell formation induced by Pg. Additionally, Pg stimulated nuclear translocation of p65, which directly bound to the promoters of CD36 to facilitate its transcription. Inhibition of p65, NF-κB or ERK1/2 blocked Pg-induced CD36 production; whereas, overexpression of NF-κB subunits p65 and p50 upregulated CD36. Furthermore, Ras inhibitors significantly attenuated ERK1/2 activation and CD36 expression. Taken together, the data indicated that stimulation of the ERK/NF-κB pathway by Pg led to transactivation of the CD36 promoters, thereby upregulating CD36 expression in the infected macrophages. These findings may help design new treatment strategies in atherosclerosis.


Asunto(s)
Infecciones por Bacteroidaceae/genética , Infecciones por Bacteroidaceae/microbiología , Antígenos CD36/genética , Sistema de Señalización de MAP Quinasas , Macrófagos Peritoneales/microbiología , FN-kappa B/metabolismo , Porphyromonas gingivalis/fisiología , Regulación hacia Arriba/genética , Animales , Infecciones por Bacteroidaceae/patología , Antígenos CD36/metabolismo , Femenino , Células Espumosas/metabolismo , Células Espumosas/microbiología , Células Espumosas/patología , Células HEK293 , Humanos , Macrófagos Peritoneales/metabolismo , Macrófagos Peritoneales/patología , Ratones , Modelos Biológicos , Regiones Promotoras Genéticas/genética , Unión Proteica , Subunidades de Proteína/metabolismo , Células RAW 264.7
19.
Yi Chuan Xue Bao ; 29(9): 791-7, 2002 Sep.
Artículo en Zh | MEDLINE | ID: mdl-12561226

RESUMEN

QTLs of three milling yield traits, including brown rice (BR,%), milled rice (MR,%) and head milled rice (HR,%), were mapped using a set of 212 Lemont/Teqing RI population, an RFLP linkage map with 182 markers and a mixed model approach(QTLMapper V1.0). The population showed continuous distributions with transgressive segregation on both sides for all traits while HR had a wider variation than BR and MR. One and four main effect QTLs were detected for MR and HR. Two QTLs for HR(QHr6 and QHr7) had large additive effects. Twelve, five and sixteen pairs of digenic epistatic loci were associated with BR, MR and HR respectively. Epistasis was more important than main effect QTLs according to the mapping result. A complex relationship was observed for epistatic pairs mapped in same trait or among different traits by sharing intervals.


Asunto(s)
Mapeo Cromosómico/métodos , Oryza/genética , Sitios de Carácter Cuantitativo/genética , Cromosomas de las Plantas/genética , Regulación de la Expresión Génica de las Plantas , Oryza/crecimiento & desarrollo
20.
Cell Signal ; 24(10): 1889-98, 2012 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-22683306

RESUMEN

The uptake of oxidized low density lipoprotein (ox-LDL) by macrophages usually leads to the formation of lipid-laden macrophages known as "foam cells," and this process plays an important role in the development of atherosclerosis. Ox-LDL activates mitogen-activated protein kinase (MAP) kinases and nuclear factor (NF)-κB, and activations of p38 and NF-κB are important for the formation of foam cells. MAP kinase phosphatase (MKP) 5 is a member of the dual specificity phosphatases (DUSPs) family that can selectively dephosphorylate activated MAPKs to regulate innate and adaptive immune responses. However, the role of MKP5 in the formation of foam cells remains unknown. Here, we found that stimulation of ox-LDL induces the expression of MKP5 in macrophages. MKP5 deficiency blocked the uptake of ox-LDL and the formation of foam cells. Further analysis revealed that deletion of MKP5 reduced the ox-LDL-induced activation of NF-κB. Also, MKP5 deficiency markedly inhibited the production of TNF-α, but enhanced the levels of TGF-ß1 in ox-LDL-stimulated macrophages. Moreover, inhibition of NF-κB by p65 RNAi significantly reduced foam cell formation in macrophages from WT mice relative to MKP5-deficient mice. Thus, MKP5 has an essential role in the formation of foam cells through activation of NF-κB, and MKP5 represents a novel target for the therapeutic intervention of atherosclerosis.


Asunto(s)
Fosfatasas de Especificidad Dual/inmunología , Células Espumosas/inmunología , Lipoproteínas LDL/inmunología , FN-kappa B/inmunología , Animales , Células Cultivadas , Fosfatasas de Especificidad Dual/genética , Quinasas MAP Reguladas por Señal Extracelular/genética , Quinasas MAP Reguladas por Señal Extracelular/inmunología , Femenino , Células Espumosas/citología , Células Espumosas/metabolismo , Regulación de la Expresión Génica , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Macrófagos/citología , Macrófagos/efectos de los fármacos , Macrófagos/inmunología , Macrófagos/metabolismo , Ratones , Ratones Endogámicos C57BL , Interferencia de ARN , Receptores Depuradores/genética , Factor de Crecimiento Transformador beta1/inmunología , Factor de Necrosis Tumoral alfa/inmunología , Regulación hacia Arriba , eIF-2 Quinasa/genética , eIF-2 Quinasa/inmunología
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