RESUMEN
Hepatocyte growth factor (HGF) serves key roles in cell motility, proliferation and immunoregulatory functions. However, the effect of HGF on macrophages is unclear. The present study aimed to elucidate the effect of HGF on the phenotypic alterations of intestinal lamina propria mononuclear cells (LPMCs). Colitis was induced in a mouse model using dextran sodium sulfate (DSS). Subsequently, LPMCs were isolated from the mice with chronic colitis and the expression levels of cytokineencoding genes in the LPMCs were determined. CD11bpositive macrophages isolated from LPMCs were cultured with HGF, and alterations in the levels of M1 or M2 markers were evaluated by reverse transcriptionquantitative polymerase chain reaction (RTqPCR) and flow cytometry. In addition, the cytokine levels were assessed using RTqPCR and ELISA. HGF shifted the phenotype of macrophages from M1 to M2like, as determined by increased mRNA expression levels of arginase1, CD206 and IL10, and reduced mRNA expression levels of CD86 and IL6 in mice with DSSinduced colitis. Moreover, HGF could ameliorate DSSinduced colitis owing to its immunosuppressive effect on immune cells. These findings indicated that HGF treatment may not only promote the regeneration of epithelial cells but also lead to tissue repair by phenotypic alteration of M1 macrophages to M2like macrophages.
Asunto(s)
Colitis , Factor de Crecimiento de Hepatocito , Ratones , Animales , Factor de Crecimiento de Hepatocito/genética , Dextranos , Colitis/inducido químicamente , Colitis/tratamiento farmacológico , Macrófagos , Modelos Animales de Enfermedad , Fenotipo , ARN Mensajero , Sulfato de Dextran/efectos adversos , Ratones Endogámicos C57BLRESUMEN
A 51-year-old man was referred to our hospital for the further examination of main pancreatic duct interruption. Imaging findings showed a 25-mm-diameter mass lesion located in the pancreatic head. Endoscopic ultrasonography (EUS)-guided fine-needle aspiration (FNA) was performed on the mass. Cytology suggested adenocarcinoma, but the histological diagnosis was not confirmed. We made a comprehensive diagnosis of resectable pancreatic cancer. The mass shrank after preoperative adjuvant chemotherapy, and the patient underwent surgery. The final pathological diagnosis was type 2 autoimmune pancreatitis (AIP). Two years after surgery, AIP had not recurred in the remaining pancreas.