Recommendations for measuring and standardizing light for laboratory mammals to improve welfare and reproducibility in animal research.

Light enables vision and exerts widespread effects on physiology and behavior, including regulating circadian rhythms, sleep, hormone synthesis, affective state, and cognitive processes. Appropriate lighting in animal facilities may support welfare and ensure that animals enter experiments in an appropriate physiological and behavioral state. Furthermore, proper consideration of light during experimentation is important both when it is explicitly employed as an independent variable and as a general feature of the environment. This Consensus View discusses metrics to use for the quantification of light appropriate for nonhuman mammals and their application to improve animal welfare and the quality of animal research. It provides methods for measuring these metrics, practical guidance for their implementation in husbandry and experimentation, and quantitative guidance on appropriate light exposure for laboratory mammals. The guidance provided has the potential to improve data quality and contribute to reduction and refinement, helping to ensure more ethical animal use.

Divergent G-protein selectivity across melanopsins from mice and humans.

Melanopsin is an opsin photopigment and light-activated G-protein-coupled receptor; it is expressed in photoreceptive retinal ganglion cells (mRGCs) and can be employed as an optogenetic tool. Mammalian melanopsins can signal via Gq/11 and Gi/o/t heterotrimeric G proteins, but aspects of the mRGC light response appear incompatible with either mode of signalling. We use live-cell reporter assays in HEK293T cells to show that melanopsins from mice and humans can also signal via Gs. We subsequently show that this mode of signalling is substantially divergent between species. The two established structural isoforms of mouse melanopsin (which differ in the length of their C-terminal tail) both signalled strongly through all three G-protein classes (Gq/11, Gi/o and Gs), whereas human melanopsin showed weaker signalling through Gs. Our data identify Gs as a new mode of signalling for mammalian melanopsins and reveal diversity in G-protein selectivity across mammalian melanopsins.

Using a bistable animal opsin for switchable and scalable optogenetic inhibition of neurons.

There is no consensus on the best inhibitory optogenetic tool. Since Gi/o signalling is a native mechanism of neuronal inhibition, we asked whether Lamprey Parapinopsin ("Lamplight"), a Gi/o-coupled bistable animal opsin, could be used for optogenetic silencing. We show that short (405 nm) and long (525 nm) wavelength pulses repeatedly switch Lamplight between stable signalling active and inactive states, respectively, and that combining these wavelengths can be used to achieve intermediate levels of activity. These properties can be applied to produce switchable neuronal hyperpolarisation and suppression of spontaneous spike firing in the mouse hypothalamic suprachiasmatic nucleus. Expressing Lamplight in (predominantly) ON bipolar cells can photosensitise retinas following advanced photoreceptor degeneration, with 405 and 525 nm stimuli producing responses of opposite sign in the output neurons of the retina. We conclude that bistable animal opsins can co-opt endogenous signalling mechanisms to allow optogenetic inhibition that is scalable, sustained and reversible.

Land-use Suitability is Not an Intrinsic Property of a Land Parcel.

Agricultural production has economic, environmental, social and cultural consequences beyond farm boundaries, but information about these impacts is not readily available to decision makers. This study applied the land use suitability concept by carrying out an assessment of a region that has the potential for intensification of agricultural production, but where eutrophication of river and estuary receiving environments due to nitrogen enrichment is a significant issue. The assessment evaluated three indicators for each farmable land parcel in the region: productive potential (the inherent productive and economic potential of the parcel), relative contribution (the potential for the parcel to contribute nitrogen to receiving environments compared to other land parcels), and pressure (the load of nitrogen delivered to receiving environments compared to the loads that ensure environmental objectives are achieved). The assessment indicated that land with high suitability for land-use intensification in Southland is limited because areas with high productive potential and low relative contribution rarely coincide with receiving environments with low pressure. Existing data, methods and models can be used to calculate the indicators under different choices for regional land-use intensity and receiving environment objectives. However, the spatial resolution and accuracy that is achievable may preclude using assessment outputs to make land use decisions at small spatial scales such as individual farms. The study highlighted that land use suitability is not an intrinsic property of a land parcel because it is dependent on choices about land use elsewhere in the landscape and the environmental objectives, and that land use suitability is inherently subjective because of decisions that concern how indicators are combined and weighted.

Quantifying the Extent of Anthropogenic Eutrophication of Lakes at a National Scale in New Zealand.

Quantifying environmental changes relative to ecosystem reference conditions (baseline or natural states) can inform assessment of anthropogenic impacts and the development of restoration objectives and targets. We developed statistical models to predict current and reference concentrations of total nitrogen (TN) and total phosphorus (TP) in surface waters for a nationally representative sample of ≥1033 New Zealand lakes. The lake-specific nutrient concentrations reflected variation in factors including anthropogenic nutrient loads, hydrology, geology, elevation, climate, and lake and catchment morphology. Changes between reference and current concentrations were expressed to quantify the magnitude of anthropogenic eutrophication. Overall, there was a clear increase in lake trophic status, with the most common trophic status being oligotrophic under a reference state and mesotrophic under current conditions. The magnitude of departure from reference state varied considerably within the sample; however, on average, the mean TN concentration approximately doubled between reference and current states, whereas the mean TP concentration increased approximately 4-fold. This study quantified the extent of water quality degradation across lake types at a national scale, thereby informing ecological restoration objectives and the potential to reduce anthropogenic nutrient loads, while also providing a modeling framework that can be applied to lakes elsewhere.

Managing Diffuse Phosphorus at the Source versus at the Sink.

Judicious phosphorus (P) management is a global grand challenge and critical to achieving and maintaining water quality objectives while maintaining food production. The management of point sources has been successful in lowering P inputs to aquatic environments, but more difficult is reducing P discharges associated with diffuse sources, such as nonpoint runoff from agriculture and urban landscapes, as well as P accumulated in soils and sediments. Strategies for effective diffuse-P management are imperative. Many options are currently available, and the most cost-effective and practical choice depends on the local situation. This critical review describes how the metrics of P quantity in kg ha-1 yr-1 and P form can influence decision-making and implementation of diffuse-P management strategies. Quantifying the total available pool of P, and its form, in a system is necessary to inform effective decision-making. The review draws upon a number of " current practice" case studies that span agriculture, cities, and aquatic sectors. These diverse examples from around the world highlight different diffuse-P management approaches, delivered at the source in the catchment watershed or at the aquatic sink. They underscore workable options for achieving water quality improvement and wider P sustainability. The diffuse-P management options discussed in this critical review are transferable to other jurisdictions at the global scale. We demonstrate that P quantity is typically highest and most concentrated at the source, particularly at farm scale. The most cost-effective and practically implementable diffuse-P management options are, therefore, to reduce P use, conserve P, and mitigate P loss at the source. Sequestering and removing P from aquatic sinks involves increasing cost, but is sometimes the most effective choice. Recovery of diffuse-P, while expensive, offers opportunity for the circular economy.

Temperature and Nitrogen Effects on Phosphorus Uptake by Agricultural Stream-Bed Sediments.

Climate change will likely increase the growing season, temperatures, and ratio of nitrogen (N) to phosphorus (P) loss from land to water. However, it is unknown how these factors influence P concentrations in streams. We sought to evaluate differences in biotic and abiotic processes affecting stream sediment P dynamics under different temperature and N-enrichment regimes. Three sediments of varying P composition and sorption characteristics were placed into a fluvarium. Synthetic runoff water, with or without added N, was added to the flume's reservoir, and the solution was maintained at 19 or 26°C. Water and sediment samples were taken with time since runoff was introduced. The rate and magnitude of P uptake by sediment was greater at 19°C compared with 26°C, and also when N was added compared with no N added. Analysis of sediment samples indicated that P uptake via abiotic processes was greater at 19 than at 26°C. The addition of N stimulated P uptake by the microbial biomass at 19°C, but microbial uptake was potentially inhibited at 26°C. Because microbial biomass is a temporary store of P, these data suggest that more P may be available with increasing temperatures during the growing season, especially under baseflow, implying that strategies to mitigate P losses from land to water should be strengthened to prevent potential water quality impairment.

Effects of Lime and Organic Amendments Derived from Varied Source Materials on Cadmium Uptake by Potato.

Repeated applications of Cd-rich phosphate fertilizers have resulted in elevated concentrations of this toxic element in some New Zealand soils. Exceedance of the food safety standard for Cd (0.1 mg kg fresh weight) has been reported for potato ( L.). Composts may efficiently sorb Cd in soil and therefore reduce its phytoavailability, leading to reduced uptake by plants. We aimed to determine the potential of various composts, shredded corn stover, and lime at two different rates to reduce the transfer of Cd from a soil (containing 1.45 mg kg Cd) to potato (var. 'Nadine'). In the control, the peeled tubers, skins, leaves, and stems had Cd concentrations of 0.04, 0.09, 0.26, and 0.53 mg kg dry weight, respectively. There was a 71% reduction in tuber Cd concentrations in potatoes grown in soil amended with 5% (w/w) shredded corn stover, although it significantly decreased potato biomass. Potatoes grown in soil amended with pig manure compost, mushroom compost, sawdust-animal waste compost, and municipal compost at rates of either 2.5 or 5% (w/w) reduced tuber Cd concentrations by 58 to 66%, 46 to 63%, 52 to 53%, and 29 to 49%, respectively. Lime (1.3%) application in soil reduced tuber Cd concentrations by 50%. Composts significantly increased tuber biomass. Further work is warranted to identify the key components of composts that result in reduced Cd uptake by plants.

Global change pressures on soils from land use and management.

Soils are subject to varying degrees of direct or indirect human disturbance, constituting a major global change driver. Factoring out natural from direct and indirect human influence is not always straightforward, but some human activities have clear impacts. These include land-use change, land management and land degradation (erosion, compaction, sealing and salinization). The intensity of land use also exerts a great impact on soils, and soils are also subject to indirect impacts arising from human activity, such as acid deposition (sulphur and nitrogen) and heavy metal pollution. In this critical review, we report the state-of-the-art understanding of these global change pressures on soils, identify knowledge gaps and research challenges and highlight actions and policies to minimize adverse environmental impacts arising from these global change drivers. Soils are central to considerations of what constitutes sustainable intensification. Therefore, ensuring that vulnerable and high environmental value soils are considered when protecting important habitats and ecosystems, will help to reduce the pressure on land from global change drivers. To ensure that soils are protected as part of wider environmental efforts, a global soil resilience programme should be considered, to monitor, recover or sustain soil fertility and function, and to enhance the ecosystem services provided by soils. Soils cannot, and should not, be considered in isolation of the ecosystems that they underpin and vice versa. The role of soils in supporting ecosystems and natural capital needs greater recognition. The lasting legacy of the International Year of Soils in 2015 should be to put soils at the centre of policy supporting environmental protection and sustainable development.

Extreme phosphorus losses in drainage from grazed dairy pastures on marginal land.

With the installation of artificial drainage and large inputs of lime and fertilizer, dairy farming can be profitable on marginal land. We hypothesized that this will lead to large phosphorus (P) losses and potential surface water impairment if the soil has little capacity to sorb added P. Phosphorous was measured in drainage from three "marginal" soils used for dairying: an Organic soil that had been developed out of scrub for 2 yr and used for winter forage cropping, a Podzol that had been developed into pasture for 10 yr, and an intergrade soil that had been in pasture for 2 yr. Over 18 mo, drainage was similar among all sites (521-574 mm), but the load leached to 35-cm depth from the Organic soil was 87 kg P ha (∼89% of fertilizer-P added); loads were 1.7 and 9.0 kg ha from the Podzol and intergrade soils, respectively. Soil sampling to 100 cm showed that added P leached throughout the Organic soil profile but was stratified and enriched in the top 15 cm of the Podzol. Poor P sorption capacity (<5%) in the Organic soil, measured as anion storage capacity, and tillage (causing mineralization and P release) in the Organic and intergrade soils were thought to be the main causes of high P loss. It is doubtful that strategies would successfully mitigate these losses to an environmentally acceptable level. However, anion storage capacity could be used to identify marginal soils with high potential for P loss for the purpose of managing risk.

The use of alum to decrease phosphorus losses in runoff from grassland soils.

Phosphorus (P) loss from land can impair surface water quality. Aluminum sulfate (alum)-treated, compared with untreated, manure or slurry decreases P loss when applied to land; our hypothesis was that alum may also decrease P loss when directly applied to grassland grazed by dairy cows. A rainfall simulation showed that alum decreased mean concentrations of filterable reactive P (FRP) by 25 to 70% and total P (TP) by 20 to 40%, depending on soil P, Al, and Fe concentration and alum application rate. Using these factors, we predicted that FRP losses would be significantly less from alum-treated grasslands than from untreated grasslands for 70 to 96 d. A 14-mo field trial compared runoff P losses from plots that received 0, 25, and 50 kg Al ha applied within a week of grazing by dairy cattle in spring. Runoff-weighted concentrations (and loads) of FRP and TP decreased in alum-treated plots by 47 to 52% and 25 to 34%, respectively. At US$157 to US$944 kg P mitigated, cost-effectiveness was estimated as medium to low compared with existing strategies for mitigating P loss in dairy farms but could be improved if applied to critical source areas of P loss. However, additional work, such as determining the need for repeat applications, is required before alum can be recommended to decrease P losses from grazed grassland.

Bayesian network for point and diffuse source phosphorus transfer from dairy pastures in South otago, new zealand.

Many factors affect the magnitude of nutrient losses from dairy farm systems. Bayesian Networks (BNs) are an alternative to conventional modeling that can evaluate complex multifactor problems using forward and backward reasoning. A BN of annual total phosphorus (TP) exports was developed for a hypothetical dairy farm in the south Otago region of New Zealand and was used to investigate and integrate the effects of different management options under contrasting rainfall and drainage regimes. Published literature was consulted to quantify the relationships that underpin the BN, with preference given to data and relationships derived from the Otago region. In its default state, the BN estimated loads of 0.34 ± 0.42 kg TP ha for overland flow and 0.30 ± 0.19 kg TP ha for subsurface flow, which are in line with reported TP losses in overland flow (0-1.1 kg TP ha) and in drainage (0.15-2.2 kg TP ha). Site attributes that cannot be managed, like annual rainfall and the average slope of the farm, were found to affect the loads of TP lost from dairy farms. The greatest loads (13.4 kg TP ha) were predicted to occur with above-average annual rainfall (970 mm), where irrigation of farm dairy effluent was managed poorly, and where Olsen P concentrations were above pasture requirements (60 mg kg). Most of this loading was attributed to contributions from overland flow. This study demonstrates the value of using a BN to understand the complex interactions between site variables affecting P loss and their relative importance.

Difficulties in using land use pressure and soil quality indicators to predict water quality.

Intensive agriculture can impair river water quality. Soil quality monitoring has been used to measure the effect of land use intensification on water quality at a point and field scales but not at the catchment scale. Other farm scale land use pressures, like stocking rate and the value of land, which relate to land use intensity are now publicly available, nationally. We therefore tested whether point scale soil quality measures, together with newly available farm scale land use pressures (land valuation and stocking rate) and existing catchment and climatic characteristics could help predict the behaviour of water quality data across 192 catchments in New Zealand. We used a generalised additive model to make predictions of the change in nitrogen fractions (r2 = 0.65-0.71), phosphorus fractions (r2 = 0.51-0.70), clarity and turbidity (r2 = 0.42-0.46), and E. coli (r2 = 0.35) over 15 years. The state and trend of water quality was strongly related to a refined farm scale land use classification, and to catchment and climatic characteristics (e.g. slope, elevation, and rainfall). Relationships with point scale soil quality measures and the land use pressures were weak. The weak relationship with land use pressures may be caused by using a single snapshot in time (2022), which cannot account for lag times in water quality response but leaves room for additional temporal data to improve predictive power. The weak relationship to soil quality measures was probably caused by limited data points (n = 667 sites) that were unrepresentative of land use, and areas of catchment subject to processes like runoff or leaching. While national soil quality measures might be useful for evaluating environmental risk at the field or farm scale, without a large increase in sampling, they were not relevant at the catchment scale. Additional analyses should be performed to determine how many samples would be needed to detect a change using an environmentally focused soil test that can guide water quality management.

A review of the development and implementation of the critical source area concept: A reflection of Andrew Sharpley's role in improving water quality.

Critical source areas (CSAs) are small areas of a field, farm, or catchment that account for most contaminant loss by having both a high contaminant availability and transport potential. Most work on CSAs has focused on phosphorus (P), largely through the work in the 1990s initiated by Dr. Sharpley and colleagues who recognized the value in targeting mitigation efforts. The CSA concept has been readily grasped by scientists, farmers, and policymakers across the globe. However, experiences and success have been mixed, often caused by the variation in where and how CSAs are defined. For instance, analysis of studies from 1990 to 2023 shows that the proportion of the annual contaminant load coming from a CSA decreases from field to farm to catchment scale. This finding is consistent with increased buffering of CSAs and greater contribution of other sources with scale, or variation in the definition of CSAs. We therefore argue that the best application of CSAs to target mitigation actions should be at small areas that truly account for most contaminant loss. This article sheds light on the development and utilization of CSAs, paying tribute to Dr. Sharpley's remarkable contributions to the improvement of water quality, and reflecting upon where the CSA concept has succeeded or not in reducing contaminant (largely P) loss.

A global dataset on phosphorus in agricultural soils.

Numerous drivers such as farming practices, erosion, land-use change, and soil biogeochemical background, determine the global spatial distribution of phosphorus (P) in agricultural soils. Here, we revised an approach published earlier (called here GPASOIL-v0), in which several global datasets describing these drivers were combined with a process model for soil P dynamics to reconstruct the past and current distribution of P in cropland and grassland soils. The objective of the present update, called GPASOIL-v1, is to incorporate recent advances in process understanding about soil inorganic P dynamics, in datasets to describe the different drivers, and in regional soil P measurements for benchmarking. We trace the impact of the update on the reconstructed soil P. After the update we estimate a global averaged inorganic labile P of 187 kgP ha-1 for cropland and 91 kgP ha-1 for grassland in 2018 for the top 0-0.3 m soil layer, but these values are sensitive to the mineralization rates chosen for the organic P pools. Uncertainty in the driver estimates lead to coefficients of variation of 0.22 and 0.54 for cropland and grassland, respectively. This work makes the methods for simulating the agricultural soil P maps more transparent and reproducible than previous estimates, and increases the confidence in the new estimates, while the evaluation against regional dataset still suggests rooms for further improvement.

Factors controlling shallow subsurface dissolved reactive phosphorus concentration and loss kinetics from poorly drained saturated grassland soils.

Shallow subsurface pathways dominate dissolved reactive phosphorus (DRP) losses in grassland soils that are: poorly drained, shallow, or have a perched water table in wetter months causing saturation-excess runoff. Saturated conditions can lead to anoxia, which can accelerate phosphorus (P) loss. Two scales of investigation were utilized in this study. First, at the field scale, soil cores were extracted to 2.5 m, subdivided and samples extracted using water extractable P (WEP) and sodium-bicarbonate-dithionite extractable P (NaBD-P). Second, at the laboratory scale, detailed incubation studies using field-moist grassland topsoils from sites in Ireland and New Zealand examined the kinetics of WEP under anoxic (WEPanox ) and oxic (WEPox ) conditions with imposed temperature and soil P fertilizer input treatments. Results from soil-core samples showed that redox-sensitive NaBD-P concentrations were depleted where artificial drainage lines were installed (100 cm deep), but WEP concentrations available to shallow flow were enriched in topsoil. The laboratory scale incubation experiment investigated the influence of temperature (3 vs. 18 °C), anoxia (designed to simulate saturation following a rainfall event), and superphosphate fertilizer (10 to 60 kg P ha-1  yr-1 ) on WEP concentrations over 24 h in three grassland topsoils (clay, silt, and sandy loam textures). Concentrations increased with fertilizer rate, temperature, and-in two soils-anoxic conditions. This was commensurate with nitrate (NO3 - ) depletion and the reductive dissolution of iron and manganese. The release of P during anoxia was complete within 24 h. The results highlighted late winter to spring as the riskiest period for topsoil P losses in shallow subsurface flow due to wet soil conditions, increasing temperatures, and low soil NO3 - concentrations. This knowledge highlights the necessity to consider and refine tests used to assess topsoil P loss risk, where in the landscape P losses are likely, and what strategies can be used to mitigate losses.

The genomic basis of temporal niche evolution in a diurnal rodent.

Patterns of diel activity-how animals allocate their activity throughout the 24-h daily cycle-play key roles in shaping the internal physiology of an animal and its relationship with the external environment.1,2,3,4,5 Although shifts in diel activity patterns have occurred numerous times over the course of vertebrate evolution,6 the genomic correlates of such transitions remain unknown. Here, we use the African striped mouse (Rhabdomys pumilio), a species that transitioned from the ancestrally nocturnal diel niche of its close relatives to a diurnal one,7,8,9,10,11 to define patterns of naturally occurring molecular variation in diel niche traits. First, to facilitate genomic analyses, we generate a chromosome-level genome assembly of the striped mouse. Next, using transcriptomics, we show that the switch to daytime activity in this species is associated with a realignment of daily rhythms in peripheral tissues with respect to the light:dark cycle and the central circadian clock. To uncover selection pressures associated with this temporal niche shift, we perform comparative genomic analyses with closely related rodent species and find evidence of relaxation of purifying selection on striped mouse genes in the rod phototransduction pathway. In agreement with this, electroretinogram measurements demonstrate that striped mice have functional differences in dim-light visual responses compared with nocturnal rodents. Taken together, our results show that striped mice have undergone a drastic change in circadian organization and provide evidence that the visual system has been a major target of selection as this species transitioned to a novel temporal niche.

Streambank erosion and phosphorus loading to surface waters: Knowns, unknowns, and implications for nutrient loss reduction research and policy.

To monitor and meet water quality objectives, it is necessary to understand and quantify the contribution of nonpoint sources to total phosphorus (P) loading to surface waters. However, the contribution of streambank erosion to surface water P loads remains unclear and is typically unaccounted for in many nutrient loading assessments and policies. As a result, agricultural contributions of P are overestimated, and a potentially manageable nonpoint source of P is missed in strategies to reduce loads. In this perspective, we review and synthesize the results of a special symposium at the 2022 ASA-CSSA-SSSA annual meeting in Baltimore, MD, that focused on streambank erosion and its contributions to P loading of surface waters. Based on discussions among researchers and policy experts, we overview the knowns and unknowns, propose next steps to understand streambank erosion contribution to P export budgets, and discuss implications of the science of streambank erosion for policy and nutrient loss reduction strategies.

Survey of Invasive Mosquito Surveillance and Control Capacity in Southeastern USA Reveals Training and Resource Needs.

Several invasive mosquito species that are nuisances or of medical and veterinary importance have been introduced into the Southeastern region of the USA, posing a threat to other species and the local ecosystems and/or increasing the risk of pathogen transmission to people, livestock, and domestic pets. Prompt and effective monitoring and control of invasive species is essential to prevent them from spreading and causing harmful effects. However, the capacity for invasive mosquito species surveillance is highly variable among mosquito control programs in the Southeast, depending on a combination of factors such as regional geography and climate, access to resources, and the ability to interact with other programs. To facilitate the development of invasive mosquito surveillance in the region, we, the Mosquito BEACONS (Biodiversity Enhancement and Control of Non-native Species) working group, conducted a survey on the capacities of various public health agencies and pest control agencies engaged in mosquito surveillance and control in seven Southeastern states (Alabama, Florida, Georgia, Louisiana, Mississippi, North Carolina, and South Carolina). Ninety control programs completed the survey, representing an overall response rate of 25.8%. We report key findings from our survey, emphasizing the training and resource needs, and discuss their implications for future invasive mosquito surveillance and control capacity building. By increasing communication and collaboration opportunities (e.g., real-time sharing of collection records, coordinated multistate programs), the establishment of Mosquito BEACONS and the implementation of this survey can accelerate knowledge transfer and improve decision support capacity in response to or in preparation for invasive mosquito surveillance and can establish infrastructure that can be used to inform programs around the world.

A review of the cost-effectiveness and suitability of mitigation strategies to prevent phosphorus loss from dairy farms in New Zealand and Australia.

The loss of phosphorus (P) from land to water is detrimental to surface water quality in many parts of New Zealand and Australia. Farming, especially pasture-based dairying, can be a source of P loss, but preventing it requires a range of fully costed strategies because little or no subsidies are available and the effectiveness of mitigation strategies varies with different farm management systems, topography, stream density, and climate. This paper reviews the cost-effectiveness of mitigation strategies for New Zealand and Australian dairy farms, grouping strategies into (i) management (e.g., decreasing soil test P, fencing streams off from stock, or applying low-water-soluble P fertilizers), (ii) amendments (e.g., alum or red mud [Bauxite residue]), and (iii) edge-of-field mitigations (e.g., natural or constructed wetlands). In general, on-farm management strategies were the most cost-effective way of mitigating P exports (cost range, $0 to $200 per kg P conserved). Amendments, added to tile drains or directly to surface soil, were often constrained by supply or were labor intensive. Of the amendments examined, red mud was cost effective where cost was offset by improved soil physical properties. Edge-of-field strategies, which remove P from runoff (i.e., wetlands) or prevent runoff (i.e., irrigation runoff recycling systems), were generally the least cost effective, but their benefits in terms of improved overall resource efficiency, especially in times of drought, or their effect on other contaminants like N need to be considered. By presenting a wide range of fully costed strategies, and understanding their mechanisms, a farmer or farm advisor is able to choose those that suit their farm and maintain profitability. Further work should examine the potential for targeting strategies to areas that lose the most P in time and space to maximize the cost-effectiveness of mitigation strategies, quantify the benefits of multiple strategies, and identify changes to land use that optimize overall dairy production, but minimize catchment scale, as versus farm scale, nutrient exports.