RESUMEN
Increasing evidence suggests that synaptic dysfunction is a key pathophysiological hallmark in neurodegenerative disorders, including Alzheimer's disease. Understanding the role of brain-derived neurotrophic factor (BDNF) in synaptic plasticity and synaptogenesis, the impact of the BDNF Val66Met polymorphism in Alzheimer's disease-relevant endophenotypes - including episodic memory and hippocampal volume - and the technological progress in measuring synaptic changes in humans all pave the way for a 'synaptic repair' therapy for neurodegenerative diseases that targets pathophysiology rather than pathogenesis. This article reviews the key issues in translating BDNF biology into synaptic repair therapies.
Asunto(s)
Factor Neurotrófico Derivado del Encéfalo/fisiología , Factor Neurotrófico Derivado del Encéfalo/uso terapéutico , Terapia Molecular Dirigida/métodos , Enfermedades Neurodegenerativas/tratamiento farmacológico , Neurogénesis/fisiología , Plasticidad Neuronal/fisiología , Sinapsis/fisiología , Animales , Factor Neurotrófico Derivado del Encéfalo/genética , Humanos , Neurogénesis/genética , Plasticidad Neuronal/genética , Polimorfismo de Nucleótido Simple/fisiología , Receptor trkB/agonistas , Receptor trkB/metabolismoRESUMEN
The somatosensory and sympathetic innervation of the vertebrate head is derived principally from the neurons of trigeminal and superior cervical ganglia (SCG), respectively. During development, the survival of both populations of neurons and the terminal growth and branching of their axons in the tissues they innervate is regulated by the supply of nerve growth factor (NGF) produced by these tissues. NGF is derived by proteolytic cleavage of a large precursor protein, proNGF, which is recognised to possess distinctive biological functions. Here, we show that proNGF promotes profuse neurite growth and branching from cultured postnatal mouse SCG neurons. In marked contrast, proNGF does not promote the growth of trigeminal neurites. Studies using compartment cultures demonstrated that proNGF acts locally on SCG neurites to promote growth. The neurite growth-promoting effect of proNGF is not observed in SCG neurons cultured from p75(NTR)-deficient mice, and proNGF does not phosphorylate the NGF receptor tyrosine kinase TrkA. These findings suggest that proNGF selectively promotes the growth of neurites from a subset of NGF-responsive neurons by a p75(NTR)-dependent mechanism during postnatal development when the axons of these neurons are ramifying within their targets in vivo.
Asunto(s)
Factor de Crecimiento Nervioso/metabolismo , Neuritas/metabolismo , Receptores de Factor de Crecimiento Nervioso/metabolismo , Animales , Células Cultivadas , Regulación del Desarrollo de la Expresión Génica , Ratones , Factores de Crecimiento Nervioso/metabolismo , Neuronas/citología , Neuronas/metabolismo , ARN Interferente Pequeño/metabolismo , Receptor trkA/metabolismo , Transducción de Señal , Factores de Tiempo , Nervio Trigémino/metabolismoRESUMEN
Formation of specific neuronal connections often involves competition between adjacent axons, leading to stabilization of the active terminal, while retraction of the less active ones. The underlying molecular mechanisms remain unknown. We show that activity-dependent conversion of pro-brain-derived neurotrophic factor (proBDNF) to mature (m)BDNF mediates synaptic competition. Stimulation of motoneurons triggers proteolytic conversion of proBDNF to mBDNF at nerve terminals. In Xenopus nerve-muscle cocultures, in which two motoneurons innervate one myocyte, proBDNF-p75(NTR) signaling promotes retraction of the less active terminal, whereas mBDNF-tyrosine-related kinase B (TrkB) p75NTR (p75 neurotrophin receptor) facilitates stabilization of the active one. Thus, proBDNF and mBDNF may serve as potential "punishment" and "reward" signals for inactive and active terminals, respectively, and activity-dependent conversion of proBDNF to mBDNF may regulate synapse elimination.
Asunto(s)
Factor Neurotrófico Derivado del Encéfalo/metabolismo , Neuronas Motoras/metabolismo , Unión Neuromuscular/metabolismo , Precursores de Proteínas/metabolismo , Transducción de Señal/fisiología , Proteínas de Xenopus/metabolismo , Animales , Factor Neurotrófico Derivado del Encéfalo/genética , Células Cultivadas , Técnicas de Cocultivo , Neuronas Motoras/citología , Fibras Musculares Esqueléticas/citología , Fibras Musculares Esqueléticas/metabolismo , Unión Neuromuscular/genética , Precursores de Proteínas/genética , Receptor trkB/genética , Receptor trkB/metabolismo , Receptores de Factor de Crecimiento Nervioso/genética , Receptores de Factor de Crecimiento Nervioso/metabolismo , Proteínas de Xenopus/genética , Xenopus laevisRESUMEN
During development, mammalian neuromuscular junctions (NMJs) transit from multiple-innervation to single-innervation through axonal competition via unknown molecular mechanisms. Previously, using an in vitro model system, we demonstrated that the postsynaptic secretion of pro-brain-derived neurotrophic factor (proBDNF) stabilizes or eliminates presynaptic axon terminals, depending on its proteolytic conversion at synapses. Here, using developing mouse NMJs, we obtained in vivo evidence that proBDNF and mature BDNF (mBDNF) play roles in synapse elimination. We observed that exogenous proBDNF promoted synapse elimination, whereas mBDNF infusion substantially delayed synapse elimination. In addition, pharmacological inhibition of the proteolytic conversion of proBDNF to mBDNF accelerated synapse elimination via activation of p75 neurotrophin receptor (p75(NTR)). Furthermore, the inhibition of both p75(NTR) and sortilin signaling attenuated synapse elimination. We propose a model in which proBDNF and mBDNF serve as potential "punishment" and "reward" signals for inactive and active terminals, respectively, in vivo.
Asunto(s)
Factor Neurotrófico Derivado del Encéfalo/fisiología , Regulación del Desarrollo de la Expresión Génica/genética , Unión Neuromuscular/metabolismo , Precursores de Proteínas/fisiología , Transducción de Señal/fisiología , Análisis de Varianza , Animales , Animales Recién Nacidos , Axones/metabolismo , Factor Neurotrófico Derivado del Encéfalo/deficiencia , Femenino , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Proteínas del Tejido Nervioso/metabolismo , Unión Neuromuscular/efectos de los fármacos , Unión Neuromuscular/crecimiento & desarrollo , Neuronas/citología , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Terminales Presinápticos/metabolismo , Inhibidores de Proteínas Quinasas/farmacología , Pirazoles/farmacología , Pirimidinas/farmacología , Receptor trkB/genética , Receptor trkB/metabolismo , Receptores de Factor de Crecimiento Nervioso/deficiencia , Transducción de Señal/efectos de los fármacos , Médula Espinal/citologíaRESUMEN
The G4C2 hexanucleotide repeat expansion in C9ORF72 is the major genetic cause of both amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) (C9-ALS/FTD). Despite considerable efforts, the development of mouse models of C9-ALS/FTD useful for therapeutic development has proven challenging due to the intricate interplay of genetic and molecular factors underlying this neurodegenerative disorder, in addition to species differences. This study presents a robust investigation of the cellular pathophysiology and behavioral outcomes in a previously described AAV mouse model of C9-ALS expressing 66 G4C2 hexanucleotide repeats. Despite displaying key molecular ALS pathological markers including RNA foci, dipeptide repeat (DPR) protein aggregation, p62 positive stress granule formation as well as mild gliosis, the AAV-(G4C2)66 mouse model in this study exhibits negligible neuronal loss, no motor deficits, and functionally unimpaired TAR DNA-binding protein-43 (TDP-43). While our findings indicate and support that this is a robust and pharmacologically tractable model for investigating the molecular mechanisms and cellular consequences of (G4C2) repeat driven DPR pathology, it is not suitable for investigating the development of disease associated neurodegeneration, TDP-43 dysfunction, gliosis, and motor performance. Our findings underscore the complexity of ALS pathogenesis involving genetic mutations and protein dysregulation and highlight the need for more comprehensive model systems that reliably replicate the multifaceted cellular and behavioral aspects of C9-ALS.
RESUMEN
Pro- and mature neurotrophins often elicit opposing biological effects. For example, mature brain-derived neurotrophic factor (mBDNF) is critical for long-term potentiation induced by high-frequency stimulation, whereas proBDNF facilitate long-term depression induced by low-frequency stimulation. Because mBDNF is derived from proBDNF by endoproteolytic cleavage, mechanisms regulating the cleavage of proBDNF may control the direction of BDNF regulation. Using methods that selectively detect proBDNF or mBDNF, we show that low-frequency stimulation induced predominant proBDNF secretion in cultured hippocampal neurons. In contrast, high-frequency stimulation preferentially increased extracellular mBDNF. Inhibition of extracellular, but not intracellular cleavage of proBDNF greatly reduced high-frequency stimulation-induced extracellular mBDNF. Moreover, high-frequency, but not low-frequency stimulation selectively induced the secretion of tissue plasminogen activator, a key protease involved in extracellular proBDNF to mBDNF conversion. Thus, high-frequency neuronal activity controls the ratio of extracellular proBDNF/mBDNF by regulating the secretion of extracellular proteases. Our study demonstrates activity-dependent control of extracellular proteolytic cleavage of a secretory protein, and reveals an important mechanism that controls diametrically opposed functions of BDNF isoforms.
Asunto(s)
Factor Neurotrófico Derivado del Encéfalo/metabolismo , Espacio Extracelular/metabolismo , Neuronas/fisiología , Animales , Western Blotting , Estimulación Eléctrica , Espacio Intracelular/metabolismo , Neuronas/citología , Neuronas/metabolismo , Isoformas de Proteínas/metabolismo , Precursores de Proteínas/metabolismo , Ratas , Activador de Tejido Plasminógeno/deficiencia , Activador de Tejido Plasminógeno/metabolismoRESUMEN
Brain-derived neurotrophic factor (BDNF) serves a pleiotropic role in the central nervous system, ranging from promoting neuronal survival and differentiation during development and synaptic modulation in the adult. An important, yet unanswered question is how BDNF could serve such diverse functions, sometimes in the same cell. At least two modes of BDNF actions have been elucidated so far based on BDNF signaling kinetics and/or the activity status of the responding neurons. Acute and gradual increases in extracellular BDNF concentrations elicit, respectively, transient and sustained activation of TrkB receptor and its downstream signaling, leading to differential molecular and cellular functions. In cultured neurons, sustained TrkB activation promotes neuronal dendritic arborization and spinogenesis, whereas transient TrkB activation facilitates dendritic growth and spine morphogenesis. In hippocampal slices, slow delivery of BDNF facilitates LTP, whereas fast application of BDNF enhances basal synaptic transmission in schaffer collateral synapses. High-frequency stimulation of neurons converts BDNF-induced TrkB signaling from a transient to a sustained mode. These initial insights lay the foundation for future investigation of the BDNF-TrkB pathway, and analogous signaling pathways to gain a comprehensive understanding to enable translational research. © 2018 Wiley Periodicals, Inc. Develop Neurobiol 78: 647-659, 2018.
Asunto(s)
Factor Neurotrófico Derivado del Encéfalo/metabolismo , Receptor trkB/metabolismo , Animales , Humanos , Neuronas/metabolismo , Transducción de Señal , Factores de TiempoRESUMEN
Although brain-derived neurotrophic factor (BDNF) has emerged as a key regulator of activity-dependent synaptic plasticity, a conceptually challenging question is how this diffusible molecule achieves local and synapse-specific modulation. One hypothesis is that neuronal activity enhances BDNF signaling by selectively modulating TrkB receptors at active neurons or synapses without affecting receptors on neighboring, less-active ones. Growing evidence suggests that neuronal activity facilitates cell-surface expression of TrkB. BDNF secreted from active synapses and neurons recruits TrkB from extrasynaptic sites into lipid rafts, microdomains of membrane that are enriched at synapses. Postsynaptic rises in cAMP concentrations facilitate translocation of TrkB into the postsynaptic density. Finally, neuronal activity promotes BDNF-induced TrkB endocytosis, a signaling event important for many long-term BDNF functions. These mechanisms could collectively underlie synapse-specific regulation by BDNF.
Asunto(s)
Encéfalo/fisiología , Plasticidad Neuronal/fisiología , Transporte de Proteínas/fisiología , Receptor trkB/metabolismo , Sinapsis/metabolismo , Animales , Factor Neurotrófico Derivado del Encéfalo/metabolismo , Humanos , Neuronas/metabolismoRESUMEN
Although late-phase long-term potentiation (L-LTP) is implicated in long-term memory, its molecular mechanisms are largely unknown. Here we provide evidence that L-LTP can be divided into two stages: an induction stage (I) and a maintenance stage (II). Both stages require mature brain-derived neurotrophic factor (mBDNF), but involve distinct underlying mechanisms. Stage I requires secretion of existing proBDNF followed by extracellular cleavage by tPA/plasmin. Stage II depends on newly synthesized BDNF. Surprisingly, mBDNF at stage II is derived from intracellular cleavage of proBDNF by furin/PC1. Moreover, stage I involves BDNF-TrkB signaling mainly through MAP kinase, whereas all three signaling pathways (phospholipase C-γ, PI3 kinase, and MAP kinase) are required for the maintenance of L-LTP at stage II. These results reveal the molecular basis for two temporally distinct stages in L-LTP, and provide insights on how BDNF modulates this long-lasting synaptic alternation at two critical time windows.
Asunto(s)
Neuronas/metabolismo , Biosíntesis de Proteínas/fisiología , eIF-2 Quinasa/metabolismo , Análisis de Varianza , Animales , Factor Neurotrófico Derivado del Encéfalo/farmacología , Células Cultivadas , Espinas Dendríticas/efectos de los fármacos , Espinas Dendríticas/metabolismo , Estimulación Eléctrica/métodos , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales Postsinápticos Excitadores/fisiología , Proteínas Fluorescentes Verdes/genética , Hipocampo/citología , Humanos , Técnicas In Vitro , Potenciación a Largo Plazo/efectos de los fármacos , Potenciación a Largo Plazo/fisiología , Ratones , Mutación/genética , Neuronas/citología , Neuronas/efectos de los fármacos , Técnicas de Placa-Clamp/métodos , Biosíntesis de Proteínas/efectos de los fármacos , Ratas , Tacrolimus/análogos & derivados , Tacrolimus/farmacología , Proteína 1A de Unión a Tacrolimus/genética , Proteínas de Unión a Tacrolimus/genética , Proteínas de Unión a Tacrolimus/metabolismo , Factores de Tiempo , Transfección/métodos , eIF-2 Quinasa/genéticaRESUMEN
Late-phase long term potentiation (L-LTP) is thought to be the cellular basis for long-term memory (LTM). While LTM as well as L-LTP is known to depend on transcription and translation, it is unclear why brain-derived neurotrophic factor (BDNF) could sustain L-LTP when protein synthesis is inhibited. The persistently active protein kinase ζ (PKMζ) is the only molecule implicated in perpetuating L-LTP maintenance. Here, in mouse acute brain slices, we show that inhibition of PKMζ reversed BDNF-dependent form of L-LTP. While BDNF did not alter the steady-state level of PKMζ, BDNF together with the L-LTP inducing theta-burst stimulation (TBS) increased PKMζ level even without protein synthesis. Finally, in the absence of de novo protein synthesis, BDNF maintained TBS-induced PKMζ at a sufficient level. These results suggest that BDNF sustains L-LTP through PKMζ in a protein synthesis-independent manner, revealing an unexpected link between BDNF and PKMζ.
Asunto(s)
Factor Neurotrófico Derivado del Encéfalo/metabolismo , Potenciación a Largo Plazo , Proteína Quinasa C/metabolismo , Animales , Regulación Enzimológica de la Expresión Génica , Ratones , Ratones Endogámicos C57BL , Neuronas/citología , Neuronas/metabolismo , Proteína Quinasa C/biosíntesis , Factores de TiempoRESUMEN
Postsynaptic cells generate positive and negative signals that retrogradely modulate presynaptic function. At developing neuromuscular synapses, prolonged stimulation of muscle cells induces sustained synaptic depression. We provide evidence that pro-brain-derived neurotrophic factor (BDNF) is a negative retrograde signal that can be converted into a positive signal by metalloproteases at the synaptic junctions. Application of pro-BDNF induces a dramatic decrease in synaptic efficacy followed by a retraction of presynaptic terminals, and these effects are mediated by presynaptic pan-neurotrophin receptor p75 (p75(NTR)), the pro-BDNF receptor. A brief stimulation of myocytes expressing cleavable or uncleavable pro-BDNF elicits synaptic potentiation or depression, respectively. Extracellular application of metalloprotease inhibitors, which inhibits the cleavage of endogenous pro-BDNF, facilitates the muscle stimulation-induced synaptic depression. Inhibition of presynaptic p75(NTR) or postsynaptic BDNF expression also blocks the activity-dependent synaptic depression and retraction. These results support a model in which postsynaptic secretion of a single molecule, pro-BDNF, may stabilize or eliminate presynaptic terminals depending on its proteolytic conversion at the synapses.
Asunto(s)
Factor Neurotrófico Derivado del Encéfalo/fisiología , Unión Neuromuscular/crecimiento & desarrollo , Terminales Presinápticos/ultraestructura , Precursores de Proteínas/fisiología , Receptor de Factor de Crecimiento Nervioso/fisiología , Transmisión Sináptica , Animales , Factor Neurotrófico Derivado del Encéfalo/metabolismo , Células Musculares/fisiología , Precursores de Proteínas/metabolismo , Sinapsis , XenopusRESUMEN
BACKGROUND: Proneurotrophins and mature neurotrophins elicit opposite effects via the p75 neurotrophin receptor (p75(NTR)) and Trk tyrosine kinase receptors, respectively; however the molecular roles of proneurotrophins in the CNS are not fully understood. RESULTS: Based on two rare single nucleotide polymorphisms (SNPs) of the human brain-derived neurotrophic factor (BDNF) gene, we generated R125M-, R127L- and R125M/R127L-BDNF, which have amino acid substitution(s) near the cleavage site between the pro- and mature-domain of BDNF. Western blot analyses demonstrated that these BDNF variants are poorly cleaved and result in the predominant secretion of proBDNF. Using these cleavage-resistant proBDNF (CR-proBDNF) variants, the molecular and cellular roles of proBDNF on the CNS neurons were examined. First, CR-proBDNF showed normal intracellular distribution and secretion in cultured hippocampal neurons, suggesting that inhibition of proBDNF cleavage does not affect intracellular transportation and secretion of BDNF. Second, we purified recombinant CR-proBDNF and tested its biological effects using cultured CNS neurons. Treatment with CR-proBDNF elicited apoptosis of cultured cerebellar granule neurons (CGNs), while treatment with mature BDNF (matBDNF) promoted cell survival. Third, we examined the effects of CR-proBDNF on neuronal morphology using more than 2-week cultures of basal forebrain cholinergic neurons (BFCNs) and hippocampal neurons. Interestingly, in marked contrast to the action of matBDNF, which increased the number of cholinergic fibers and hippocampal dendritic spines, CR-proBDNF dramatically reduced the number of cholinergic fibers and hippocampal dendritic spines, without affecting the survival of these neurons. CONCLUSION: These results suggest that proBDNF has distinct functions in different populations of CNS neurons and might be responsible for specific physiological cellular processes in the brain.
Asunto(s)
Factor Neurotrófico Derivado del Encéfalo/metabolismo , Sistema Nervioso Central/citología , Neuritas/metabolismo , Precursores de Proteínas/metabolismo , Sustitución de Aminoácidos/genética , Animales , Especificidad de Anticuerpos/efectos de los fármacos , Apoptosis/efectos de los fármacos , Transporte Biológico/efectos de los fármacos , Factor Neurotrófico Derivado del Encéfalo/química , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Sistema Nervioso Central/metabolismo , Biología Computacional , Medios de Cultivo , Hipocampo/efectos de los fármacos , Hipocampo/metabolismo , Humanos , Espacio Intracelular/efectos de los fármacos , Espacio Intracelular/metabolismo , Ratones , Proteínas Mutantes/metabolismo , Neuritas/efectos de los fármacos , Polimorfismo de Nucleótido Simple/genética , Potasio/farmacología , Precursores de Proteínas/química , Procesamiento Proteico-Postraduccional/efectos de los fármacos , Estructura Secundaria de Proteína , Ratas , Proteínas Recombinantes/farmacologíaRESUMEN
Pro-brain-derived neurotrophic factor (proBDNF) and mature BDNF utilize distinct receptors to mediate divergent neuronal actions. Using new tools to quantitate endogenous BDNF isoforms, we found that mouse neurons secrete both proBDNF and mature BDNF. The highest levels of proBDNF and p75 were observed perinatally and declined, but were still detectable, in adulthood. Thus, BDNF actions are developmentally regulated by secretion of proBDNF or mature BDNF and by local expression of p75 and TrkB.
Asunto(s)
Factor Neurotrófico Derivado del Encéfalo/metabolismo , Neuronas/metabolismo , Precursores de Proteínas/metabolismo , Animales , Anticuerpos Monoclonales , Factor Neurotrófico Derivado del Encéfalo/genética , Factor Neurotrófico Derivado del Encéfalo/inmunología , Supervivencia Celular/fisiología , Células Cultivadas , Regulación del Desarrollo de la Expresión Génica , Técnicas de Sustitución del Gen , Hipocampo/citología , Ratones , Ratones Mutantes , Neuronas/citología , Precursores de Proteínas/genética , Precursores de Proteínas/inmunología , Receptor de Factor de Crecimiento Nervioso/metabolismo , Receptor trkB/metabolismoRESUMEN
While Trk receptors can be activated in a neurotrophin-independent manner through "transactivation" by GPCR ligands, its physiological significance in the brain remains unknown. Huang et al. have now identified a novel mechanism of TrkB transactivation. They show that zinc ions can transactivate TrkB independent of neurotrophins and that such a transactivation is important for mossy fiber long-term potentiation (LTP).
Asunto(s)
Plasticidad Neuronal/fisiología , Receptor trkB/biosíntesis , Sinapsis/metabolismo , Activación Transcripcional/fisiología , Zinc/metabolismo , Animales , Humanos , Receptor trkB/genética , Sinapsis/genéticaRESUMEN
The metabotropic glutamate receptor 3 (GRM3, mGluR3) is important in regulating synaptic glutamate. Here, we report the existence of three splice variants of GRM3 in human brain arising from exon skipping events. The transcripts are expressed in prefrontal cortex, hippocampus and cerebellum, and in B lymphoblasts. We found no evidence for alternative splicing of GRM2. The most abundant GRM3 variant lacks exon 4 (GRM3Delta4). In silico translation analysis of GRM3Delta4 predicts a truncated protein with a conserved extracellular ligand binding domain, absence of a seven-transmembrane domain, and a unique 96-amino acid C-terminus. When expressed in rat hippocampal neurons, GRM3Delta4 is translated into a 60 kDa protein. Immunostaining and cell fractionation data indicate that the truncated protein is primarily membrane-associated. An antibody developed against the GRM3Delta4 C-terminus detects a protein of approximately 60 kDa in human brain lysates and in B lymphoblasts, suggesting translation of GRM3Delta4 in vivo. The existence of the GRM3Delta4 isoform is relevant in the light of the reported association of non-coding single nucleotide polymorphisms (SNPs) in GRM3 with schizophrenia, and with the potential of GRM3 as a therapeutic target for several neuropsychiatric disorders.