RESUMEN
The primary test for the laboratory confirmation of rubella is IgM serology. It is important to distinguish IgM reactivity caused by primary infection from that caused by reinfection or persistence, especially in pregnant women; as termination of pregnancy is considered when primary rubella is diagnosed during the first trimester. In this study, the performance of rubella IgM enzyme immunoassay (IgM-EIA) and rubella IgG avidity assay were compared using well-defined panels of sera from persons vaccinated against rubella and commercial rubella IgM and IgG enzyme immunoassay kits (Dade Behring, Marburg, Germany). The sensitivity and specificity of rubella IgM-EIA were found to be 77.4 and 97.9%, respectively, while the results for rubella IgG avidity assay were 100 and 100%. IgG avidity assay showed higher positive and negative predictive values than the IgM-EIA (100 and 100% compare to 96.9 and 82.9%). In conclusion, the rubella IgG avidity assay is more sensitive and specific than IgM-EIA for differential detection of primary rubella infection from rubella reinfection.
Asunto(s)
Ensayo de Inmunoadsorción Enzimática/métodos , Inmunoglobulina G/inmunología , Inmunoglobulina M/sangre , Juego de Reactivos para Diagnóstico , Vacuna contra la Rubéola/administración & dosificación , Virus de la Rubéola/inmunología , Rubéola (Sarampión Alemán)/inmunología , Vacunación , Afinidad de Anticuerpos , Humanos , Inmunoglobulina G/sangre , Rubéola (Sarampión Alemán)/sangre , Factores de TiempoRESUMEN
BACKGROUND: Prediction of the onset of menstruation (menarche age) using height, weight and Body Mass Index (BMI) is a major health procedure. The present study was conducted to determine the relationship between anthropometric indices and menarche age in 488 girls 11-17 years in southern Iran (Kish Island) in 2011. METHODS: Data was collected using questionnaires as well as measurements of the children's height and weight. This data was analyzed using t-test and logistic regression. RESULTS: Median age of menarche of menstruated girls as inferred from the age of menarche cumulative distribution was 12.9 years. Mean (SD) BMI in menstruated and non-menstruated girls were 21.97 (4.5) and 19.17 (3.7), respectively. Mean (SD) weight and height of the menstruated girls were 53.65 (12.3) kg and 156.06 (5.5) cm, respectively which are higher than respective figures of the non-menstruated participants 43.70 (10.7) kg and 150.21 (6.3) cm, respectively. Our results revealed a significant correlation between BMI and menarche age. CONCLUSION: Menarche age and BMI are significantly correlated with higher BMI related to lower menarche age.