RESUMEN
Spondylodiscitis caused by Campylobacter species is a rare disease which is most often caused by Campylobacter fetus. We report a case of culture-negative spondylodiscitis and a psoas abscess due to Campylobacter jejuni in a 68-year-old woman, as revealed by 16S rRNA gene and Campylobacter-specific PCRs from biopsied tissue.
Asunto(s)
Infecciones por Campylobacter/diagnóstico , Campylobacter jejuni/genética , Discitis/diagnóstico , Anciano , Infecciones por Campylobacter/microbiología , Discitis/microbiología , Femenino , Humanos , Técnicas de Diagnóstico Molecular , Reacción en Cadena de la Polimerasa , ARN Bacteriano/genética , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADNRESUMEN
Visceral pentastomiasis caused by Armillifer armillatus larvae was diagnosed in 2 dogs in The Gambia. Parasites were subjected to PCR; phylogenetic analysis confirmed relatedness with branchiurans/crustaceans. Our investigation highlights transmission of infective A. armillatus ova to dogs and, by serologic evidence, also to 1 human, demonstrating a public health concern.
Asunto(s)
Enfermedades de los Perros/transmisión , Enfermedades Parasitarias en Animales/transmisión , Enfermedades Parasitarias/transmisión , Pentastomida/fisiología , Serpientes/parasitología , Zoonosis/transmisión , Adulto , Crianza de Animales Domésticos , Animales , Anticuerpos/sangre , Niño , Enfermedades de los Perros/diagnóstico , Enfermedades de los Perros/parasitología , Perros , Femenino , Gambia , Humanos , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Óvulo/fisiología , Enfermedades Parasitarias/diagnóstico , Enfermedades Parasitarias/parasitología , Enfermedades Parasitarias en Animales/diagnóstico , Enfermedades Parasitarias en Animales/parasitología , Pentastomida/genética , Pentastomida/crecimiento & desarrollo , Pentastomida/inmunología , ARN Ribosómico 18S/genética , Análisis de Secuencia de ADN , Zoonosis/parasitologíaRESUMEN
A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-beta-lactamase (MBL) production. Molecular analysis detected a blaVIM-1 gene in the chromosome of one isolate and a blaVIM-2 gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory EDTA combination disk test represents a valid alternative to the molecular investigation of MBL genes, making MBL detection possible in routine diagnostic laboratories.
Asunto(s)
Hospitales Universitarios/estadística & datos numéricos , Infecciones por Pseudomonas/epidemiología , Pseudomonas aeruginosa/efectos de los fármacos , Pseudomonas aeruginosa/enzimología , beta-Lactamasas/metabolismo , Antibacterianos/farmacología , Ceftazidima/farmacología , Farmacorresistencia Bacteriana , Alemania/epidemiología , Humanos , Imipenem/farmacología , Pruebas de Sensibilidad Microbiana , Infecciones por Pseudomonas/microbiología , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/aislamiento & purificación , beta-Lactamasas/genéticaRESUMEN
Tobramycin and colistin represent 2 standard antimicrobial agents in the treatment of cystic fibrosis (CF) patients who are chronically colonized with Pseudomonas aeruginosa. In this study, we determined the rate of resistance to tobramycin and colistin in 1844 isolates of P. aeruginosa obtained from 22 CF patients under alternate therapy with inhaled tobramycin and colistin. Resistance to tobramycin was observed in 27.5% of isolates. In contrast, all isolates were susceptible to colistin. Molecular typing of selected isolates suggested that only 1 clone occurred over time in each patient. To conclude, resistance to tobramycin in P. aeruginosa isolates from CF patients under antimicrobial therapy may occur while colistin resistance remains uncommon.
Asunto(s)
Antibacterianos/farmacología , Colistina/farmacología , Fibrosis Quística/complicaciones , Farmacorresistencia Bacteriana , Infecciones por Pseudomonas/microbiología , Pseudomonas aeruginosa/efectos de los fármacos , Tobramicina/farmacología , Adulto , Técnicas de Tipificación Bacteriana , Enfermedad Crónica , Análisis por Conglomerados , Fibrosis Quística/tratamiento farmacológico , Dermatoglifia del ADN , Femenino , Genotipo , Humanos , Masculino , Infecciones por Pseudomonas/tratamiento farmacológico , Pseudomonas aeruginosa/clasificación , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/aislamiento & purificaciónRESUMEN
The objective of this study was to characterize the antimicrobial resistance patterns of 100 clinical isolates of Enterobacter spp. with special regard to the occurrence of extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance by qnr-determinants. The rate of ESBL- and qnr-positive isolates was 7% and 14%, respectively. Thirteen isolates harbored a qnrA1, and a further isolate harbored a qnrB4 gene. Moreover, qnr-determinants were significantly associated with ESBL-expression. No carbapeneme or tigecycline resistance was detected in the collective tested. To conclude, these data confirm the increase of multiple antimicrobial resistance mechanisms in Enterobacter spp.
Asunto(s)
Antibacterianos/farmacología , Enterobacter/efectos de los fármacos , Quinolonas/farmacología , beta-Lactamasas/genética , Proteínas Bacterianas/genética , Farmacorresistencia Bacteriana/genética , Enterobacter/genética , Enterobacter/aislamiento & purificación , Infecciones por Enterobacteriaceae/tratamiento farmacológico , Infecciones por Enterobacteriaceae/microbiología , Alemania/epidemiología , Humanos , Pruebas de Sensibilidad Microbiana , PlásmidosRESUMEN
Skin and soft tissue infections caused by Panton-Valentine leukocidin-producing strains of Staphylococcus aureus are emerging among travelers returning from the tropics. Here, we here present data on 15 affected individuals. Intrafamiliar spread was documented in one case, and occupational transmission was assumed in another. Spa typing of the strains revealed a broad spectrum of variants, but some were clonally related. Methicillin-resistant Staphylococcus aureus (MRSA) was found in three cases.