Impacts of fungal entomopathogens on survival and immune responses of Aedes albopictus and Culex pipiens mosquitoes in the context of native Wolbachia infections.

Microbial control of mosquitoes via the use of symbiotic or pathogenic microbes, such as Wolbachia and entomopathogenic fungi, are promising alternatives to synthetic insecticides to tackle the rapid increase in insecticide resistance and vector-borne disease outbreaks. This study evaluated the susceptibility and host responses of two important mosquito vectors, Ae. albopictus and Cx. pipiens, that naturally carry Wolbachia, to infections by entomopathogenic fungi. Our study indicated that while Wolbachia presence did not provide a protective advantage against entomopathogenic fungal infection, it nevertheless influenced the bacterial / fungal load and the expression of select anti-microbial effectors and phenoloxidase cascade genes in mosquitoes. Furthermore, although host responses from Ae. albopictus and Cx. pipiens were mostly similar, we observed contrasting phenotypes with regards to susceptibility and immune responses to fungal entomopathogenic infection in these two mosquitoes. This study provides new insights into the intricate multipartite interaction between the mosquito host, its native symbiont and pathogenic microbes that might be employed to control mosquito populations.

Learning and behavior in hatchling Trachemys scripta exposed to bisphenol-a during embryonic development.

Steroids play an integral role in orchestrating embryonic development, and they can affect a suite of phenotypic traits, including learning and behavior. Endocrine disrupting compounds (EDCs) can alter steroid-dependent phenotypic traits during embryonic development. Bisphenol-A (BPA) is an EDC that disrupts the action of estrogen, and recent work indicates that BPA can affect learning and behavior similarly to estrogen. We exposed red-eared slider turtle (Trachemys scripta) eggs to BPA during embryonic development and tested hatchlings for effects on learning and behavior in modified T-mazes over the course of two weeks. We found that behavioral patterns changed within a day and over the course of the experiment, but we found no effect of BPA treatment. Further, we found that hatchling turtles were highly consistent in their behaviors. These behaviors varied among individuals, suggesting that there are discrete behavioral types in T. scripta hatchlings. The highly repetitive nature of behaviors in the hatchlings might explain the innate biases that we observed and warrants further study.