Rosai-Dorman disease mimicking lymphoma on 18F-FDG PET/CT.

Rosai-Dorman Disease (RDD) is an uncommon benign disorder of proliferative histiocytes. RDD is usually self-remitting yet has been shown to mimic lympho-proliferative disorders clinically as well as on imaging studies. We report a-47-year-old female who was referred to our department with the presenting complaint of bulky abdominopelvic nodal masses suspicious of lymphoma where 18F-FDG PET/CT played a role in the evaluation and staging.

Are polyploid giant cancer cells in high grade serous carcinoma of the ovary blastomere-like cancer stem cells?

Polyploid giant cancer cells, either multinucleated or mononucleated, in high grade serous carcinoma of the ovary have been previously recognized. Different theories including degenerative changes or an important step in the development of high grade serous carcinoma have been proposed. Here we investigate possible explanations for the presence of polyploid giant cancer cells in high grade serous carcinoma. We reviewed 33 cases of ovarian high grade serous carcinoma (12 stage I, 7 stage II, and 14 stage III). We counted the number of polyploid giant cancer cells in 20 consecutive 10× fields. In 11 cases where polyploid giant cancer cells were easily found, immunohistochemistry for Ki67, p53, and OCT 3/4 was performed. Patients with polyploid giant cancer cells were older than those without. Polyploid giant cancer cells were more frequent in stage I lesions (75%) than in stages II or III (57% in both) and less frequent in metastases compared with primary ovarian tumors. Mitotic figures were present in regular sized cells but were absent in polyploid giant cancer cells. OCT3/4 was negative in all cases assessed. In 8 cases, more than 70% of the mononuclear cells were positive for Ki-67, similar to the percentage of Ki-67 positive cells in polyploid giant cancer cells. p53 had a perfect correlation in regular sized cancer cells and in polyploid giant cancer cells. Polyploid giant cancer cells are neither degenerative cells nor traditional cancer stem cells but most probably represent an intermediate step between stem cells and mature tumor cells formed by endoreplication.

Histology of the normal ovary in premenopausal patients.

Detailed descriptions of ovarian histology are rare. We reviewed in detail 57 cases of normal ovaries in premenopausal patients, when the ovaries are active and primordial follicles are found. We also proposed updated definitions to more clearly distinguish inclusion cysts, which do not have a known relationship with any disease process, from endosalpingiosis, a lesion closely associated with low grade serous neoplasia of the ovary. The most interesting findings were the significant variation in the histologic features including the variation in the amount and the distribution of primordial follicles, follicular cysts, and endosalpingiosis, within the ovary and between both ovaries in the same patient, the frequent presence of primordial follicles in the medulla, specifically in cases of multiple follicular cysts, and the frequent presence of endosalpingiosis. We believe that to confirm a pathologic process in the ovary, we need to become familiar with the histologic features of the normal ovary and their variations.

Navigating the Challenges Associated With a Diagnosis of Autoimmune Pancreatitis and IgG4-Related Sclerosing Cholangitis.

CONTEXT.­: The pancreatobiliary tract exhibits a spectrum of heterogeneous fibroinflammatory conditions that may be the result of a primary immune-mediated mechanism, or a reaction to neoplasm. This often results in significant overlap regarding clinical presentation, symptoms, radiographic findings, serology, and histopathology between inflammatory and neoplastic lesions of the pancreas, which can lead to inadvertent surgical intervention. Among the multitude of primary fibroinflammatory pancreatic diseases, autoimmune pancreatitis, including type 1 and type 2 autoimmune pancreatitis, and immunoglobulin G4-related sclerosing cholangitis (IgG4-RSC) are particularly challenging and require a multidisciplinary perspective to reliably make a diagnosis. This is of particular significance because these diseases typically have a favorable prognosis and readily respond to steroid therapy. OBJECTIVE.­: To present a multimodal approach to highlight distinctive and overlapping qualities that will aid in the diagnosis of these entities. DATA SOURCES.­: The review and analysis of literature describing autoimmune pancreatitis types 1 and 2 and IgG4-RSC. CONCLUSIONS.­: Diagnosis of autoimmune pancreatitis types 1 and 2 and IgG4-RSC requires a multimodal approach that relies on clinical, radiographic, serologic, histopathologic, and immunohistochemical correlation.

FIP1L1-PDGFRA fusion gene in T-lymphoblastic lymphoma: A case report.

BACKGROUND: T-lymphoblastic lymphoma (T-LBL) is an aggressive malignancy of T-lymphoid precursors, rarely co-occurring with myeloid/lymphoid neoplasms with eosinophilia (M/LNs-Eo), with consequent rearrangement of tyrosine kinase (TK)-related genes. The FIP1L1-PDGFRA fusion gene is the most frequent molecular abnormality seen in eosinophilia-associated myeloproliferative disorders, but is also present in acute myeloid leukemia (AML), T-lymphoblastic leukemia/lymphoma (TLL), or both simultaneously. T-LBL mainly affects children and young adults, involving lymph node, bone marrow, and thymus. It represents about 85% of all immature lymphoblastic lymphomas, whereas immature B-cell lymphomas comprise approximately 15% of all cases of LBL. CASE: In this case report, we present an example of T cell lymphoblastic lymphoma with coexistent eosinophelia, treated successfully with a tyrosine-kinase inhibitor (TKI). CONCLUSION: FIP1L1-PDGFRA-positive T-LBL and myeloproliferative disorders have excellent response to low-dose treatment with (TKI) imatinib. Most patients achieve rapid and complete hematologic and molecular remission within weeks.

Kazachstania slooffiae, an emerging pathogen to watch for in humans?

In an 80-year-old man with long-term dysphagia, an upper endoscopy was performed and biopsy samples collected for microbiological and pathological tests, showing fungal structures. Kazachstania slooffiae was isolated in microbiological cultures that were later confirmed with DNA sequencing. Susceptibility tests were performed, and antifungal treatment was initiated with a clinical, pathological, and microbiological response.

Giant cell-rich solitary fibrous tumour of the lacrimal gland with prominent angiomatoid cystic changes and an underlying NAB2ex3-STAT6ex18 fusion.

Giant cell-rich solitary fibrous tumour (GCR-SFT) is a rare variant of SFT with a predilection for the orbital region. Despite its hypervascularity, extensive angiomatoid cystic changes are unusual in GCR-SFT and may pose a diagnostic challenge. A 47-year-old man presented with a right eye proptosis and a protruding tumour of several years' duration with recently accelerated tumour growth. MRI revealed a cystic-solid heterogeneous mass arising from the lacrimal gland and displacing the globe. A subtotal excision of the mass was performed due to unanticipated hypervascularity and intraoperative bleeding. Pathologically, a vascular neoplasm was initially suspected. The diagnosis of GCR-SFT was made following careful evaluation and demonstration of CD34 and STAT6 expression. Molecular studies revealed a pathognomonic but rare NAB2ex3-STAT6ex18 fusion variant as well as a TP53 mutation suggestive of aggressive phenotype. The patient had a complete resolution of the proptosis but the clinical picture remains guarded due to incomplete resection.

Primary Mediastinal Large B-cell Lymphoma : Impact of Chemotherapy Choice.

OBJECTIVE/BACKGROUND: Data generated from retrospective studies on primary mediastinal B-cell lymphoma (PMBCL) outcome are valuable as no prospective phase 3 trials have been conducted in this rare type of lymphoma. METHODS: Our goal was to assess the long-term outcome of 41 patients with PMBCL who were treated at the Kuwait Cancer Center. We evaluated two types of multidrug treatment, R-CHOP (rituximab, vincristine, doxorubicin, cyclophosphamide, and prednisone) and DA-EPOCH-R (etoposide, prednisone, vincristine, cyclophosphamide, doxorubicin, and rituximab), and determined overall survival and complete response (CR) as primary endpoints. RESULTS: In our cohort, 27 (66%) cases were treated with R-CHOP and 14 (34%) cases were treated with DA-EPOCH-R. The overall median follow-up time was 34 months. Among the patients treated with R-CHOP, 23 out of 27 (92.6%) patients achieved CR; similarly, 10 out of 14 patients (85.7%) in the DA-EPOCH-R group achieved CR after initial treatment. There were no differences in OS between patients treated with R-CHOP versus DA-EPOCH-R. CONCLUSION: The findings of this study indicate that combined chemotherapy and immunotherapy results in excellent long-term outcome of patients with PMBCL. At our center, we prefer R-CHOP to DA-EPOCH-R for low-risk patients with nonbulky disease.

Clinicopathological Analysis of Uterine Allografts Including Proposed Scoring of Ischemia Reperfusion Injury and T-cell-mediated Rejection-Dallas UtErus Transplant Study: A Pilot Study.

BACKGROUND: Uterus transplantation (UTx) enables pregnancy in infertile women. This study describes the histopathological changes of ischemia reperfusion injury and mostly acute T-cell-mediated rejection (TCMR) in UTx and proposes modification toward a working formulation grading system with associated treatments. METHODS: Protocol and indication biopsies from 11 living and 2 deceased donor UTx recipients were analyzed. Serving as a control were 49 age-matched nontransplanted uteri. All posttransplant histopathological specimens were evaluated in a blinded fashion by 3 pathologists. Response to treatment was assessed by follow-up biopsies. Serial serum donor-specific antibody (DSA) responses were also recorded. RESULTS: Changes attributed to ischemia reperfusion resolved within 2 wk of UTx in most of the patients. For TCMR grading, perivascular inflammation, focal capillary disruption, and interstitial hemorrhage were added to interface inflammation, intercellular edema, stromal inflammation, and epithelial apoptotic bodies. Of the 173 protocol biopsies, 98 were classified as negative for TCMR; 34 as indeterminate-borderline; 35 as mild; 3 as moderate; and 3 as severe, 1 of which occurred in a DSA-positive recipient and also showed microvascular injury. Corticosteroids successfully treated all moderate-to-severe TCMR episodes. Mild TCMR was treated by increasing existing baseline immunosuppression. Indeterminate-borderline episodes were not treated. Neither ischemia-reperfusion injury nor TCMR with DSA adversely affected embryo transfer. CONCLUSIONS: Relying on a modified histopathological grading system, we developed a treatment strategy resulting in resolution of TCMR and successful pregnancies.

Gene of the month: TLE 1.

TLE 1 is the human homologue belonging to a family of four genes and is located on chromosome 9q21. It consists of 19 exons. Although it does not bind directly to DNA, it acts as a repressor of several signalling pathways via transcription factors. TLE1 protein has several physiological roles in embryogenesis, haematopoiesis, general differentiation, and both neuronal and eye development. Much attention was focused on its expression in the tumour cell nuclei of synovial sarcoma (SS). However, several other soft tissue tumours that do and do not share morphological similarity with SS also display nuclear immunoreactivity for TLE1; hence, caution in interpretation is advocated.

Gene of the month: TFE 3.

Transcription factor enhancer 3 (TFE3), on the short arm of chromosome Xp11.23 and its protein, belongs to the microphthalmia transcription family (MiTF) of transcription factors. It shares close homology with another member of the family, MiTF which is involved in melanocyte development. When a cell is stressed and/or starved, TFE3 protein translocates into the nucleus. TFE3 gene fusions with multiple different partner genes occur in several tumours with resultant nuclear expression of TFE3 protein. The main tumours associated with TFE3 gene fusions are: renal cell carcinoma, alveolar soft part sarcoma, a subset of epithelioid haemangioendotheliomas (EHE), some perivascular epithelioid cell tumours and rare examples of ossifying fibromyxoid tumour and malignant chondroid syringoma. TFE3 immunohistochemistry is of use in routine diagnostic practice with the aforementioned tumours harbouring TFE3 fusions leading to nuclear staining. In addition, there are tumours lacking TFE3 fusions but also display TFE3 nuclear immunolabeling, and these include: granular cell tumour, solid pseudopapillary neoplasm of the pancreas and ovarian sclerosing stromal tumour.

Gene of the month: GLIS1-3.

The GLIS 1-3 genes belong to a family of transcription factors, the Krüppel-like zinc finger proteins. The GLIS proteins function primarily as activators of transcription (GLIS 1 and 3), while GLIS 2 functions as a repressor. Collectively, the GLIS proteins are involved in a variety of diseases in several organs ranging from Alzheimer's disease, facial dysmorphism, neonatal diabetes mellitus, breast and colon cancers and leukaemia. In particular, loss-of-function mutations in GLIS2 are responsible for an autosomal recessive cystic kidney disease called nephronophthisis, which is characterised by tubular atrophy, interstitial fibrosis and corticomedullary cysts.Of diagnostic value in current practice are the presence of GLIS 3 and 1 fusions with PAX8 in almost 100% of hyalinising trabecular tumours of the thyroid gland. This enables its separation from papillary thyroid cancer.

Promising effect of PDL1 inhibitors in the front-line management of primary aggressive central nervous system lymphoma: A case report.

Primary central nervous system lymphoma (PCNSL) is a rare lymphoma that involves the central nervous system. The standard treatment involves chemotherapy with high-dose methotrexate. To the best of our knowledge, this is the first reported case of employing checkpoint inhibitor, nivolumab, alone to treat a patient with PCNSL who could not tolerate the induction therapy. In aggressive cases of PCNSL where chemotherapy may become futile, stand-alone checkpoint inhibitors should be considered as the front-line treatment protocol.

Encefalitis asociada a infección por SARS-CoV-2 / Encephalitis associated with SARS-CoV-2 infection / Encefalite associada à infecção por SARS-CoV-2

El virus SARS-CoV-2 produce una enfermedad conocida como COVID-19 y puede producir complicaciones neurológicas como la encefalitis, la cual consiste en la inflamación a nivel del parénquima cerebral. Su pronto diagnóstico es crucial para poder asegurar la supervivencia de los individuos, ya que puede llevar al paciente al ingreso en unidad de cuidados intensivos. El tratamiento consiste en el soporte vital, la disminución de la inflamación y de la presión intracraneal, aunque estas medidas en ocasiones no son suficientes debido a que posee una alta tasa de mortalidad. OBJETIVO: identificar las principales características clínicas de la encefalitis asociada a la infección por SARS-CoV-2. METODOLOGIA: se realizó una revisión sistemática bajo la metodología PRISMA, utilizando diversos motores de búsqueda como PubMed, ScienceDirect, Web of Science y Scopus de los últimos cinco años en idioma inglés y español. RESULTADOS: se encontraron 63 artículos identificados en las bases de datos: PubMed; 18, Scielo con un total de 3, Sciencedirect con 3 y Google Scholar; 39. De estos artículos encontrados, 15 artículos estaban duplicados, 13 artículos eliminados por título y resumen, esto realizado luego de tomar en cuenta criterios de exclusión y relevancia del artículo mismo, se eliminaron 25 artículos luego de analizar el texto completo, obteniendo finalmente 10 artículos a emplear dentro del presente estudio. CONCLUSION: se concluyó que el SARS-CoV-2 tiene repercusión a nivel del sistema nervioso central, dando como resultado la presencia de patologías como encefalitis, la cual tiene una baja incidencia entre los pacientes, pero una mortalidad para nada despreciable.

The SARS-CoV-2 virus produces a disease known as COVID-19 and can produce neurological complications such as encephalitis, which consists of inflammation at the level of the brain parenchyma. Early diagnosis is crucial to ensure the survival of individuals, as it can lead to admission to the intensive care unit. Treatment consists of life support, reduction of inflammation and intracranial pressure, although these measures are sometimes not sufficient due to a high mortality rate. Objective. To identify the main clinical features of encephalitis associated with SARS-CoV-2 infection. Methodology. A systematic review was carried out under the PRISMA methodology, using different search engines such as PubMed, ScienceDirect, Web of Science and Scopus from the last five years in English and Spanish. Results. We found 63 articles identified in the databases: PubMed; 18, Scielo with a total of 3, Sciencedirect with 3 and Google Scholar; 39. Of these articles found, 15 articles were duplicates, 13 articles eliminated by title and abstract, this done after taking into account exclusion criteria and relevance of the article itself, 25 articles were eliminated after analyzing the full text, finally obtaining 10 articles to be used within the present study. Conclusion. It was concluded that SARS-CoV-2 has repercussions at the level of the central nervous system, resulting in the presence of pathologies such as encephalitis, which has a low incidence among patients, but not negligible mortality.

O vírus SARS-CoV-2 causas uma doença conhecida como COVID-19 e pode levar a complicações neurológicas, como a encefalite, que consiste em uma inflamação no nível do parênquima cerebral. O diagnóstico precoce é fundamental para garantir a sobrevivência dos indivíduos, pois pode levar à internação na unidade de terapia intensiva. O tratamento consiste em suporte à vida, redução da inflamação e redução da pressão intracraniana, embora essas medidas às vezes não sejam suficientes devido à alta taxa de mortalidade. Objetivo. Identificar as principais características clínicas da encefalite associada à infecção pelo SARS-CoV-2. Metodologia. Foi realizada uma revisão sistemática de acordo com a metodologia PRISMA, usando vários mecanismos de busca, como PubMed, ScienceDirect, Web of Science e Scopus, dos últimos cinco anos, em inglês e espanhol. Resultados. Sessenta e três artigos foram identificados nos seguintes bancos de dados: PubMed; 18, Scielo com um total de 3, Sciencedirect com 3 e Google Scholar; 39. Desses artigos encontrados, 15 eram duplicatas, 13 artigos foram eliminados pelo título e resumo, o que foi feito após levar em conta os critérios de exclusão e a relevância do artigo em si, 25 artigos foram eliminados após a análise do texto completo, obtendo-se finalmente 10 artigos a serem usados no presente estudo. Conclusões. Concluiu-se que o SARS-CoV-2 tem repercussões em nível do sistema nervoso central, resultando na presença de patologias como a encefalite, que tem baixa incidência entre os pacientes, mas mortalidade não desprezível.

Mullerian adenosarcoma of the cervix with heterologous elements and sarcomatous overgrowth.

Cervical adenosarcomas are exceedingly infrequent tumors that occur most often in women of reproductive age. Adenosarcomas comprise benign epithelial elements and malignant stromal elements. The malignant stromal elements can either be homologous, such as fibroblasts or smooth muscle, or heterologous, like cartilage, striated muscle, or bone. We report a case of adenosarcoma of the cervix with heterologous elements and sarcomatous overgrowth in a 38-year-old woman.

Cervical dysplasia in adolescents.

BACKGROUND: Although the incidence of cervical dysplasia in adolescents is increasing, a paucity of data exists regarding the outcomes of adolescents with Pap test abnormalities. We determined the natural history and outcome of adolescents with low-grade squamous intraepithelial lesions (LSIL) and high-grade squamous intraepithelial lesions (HSIL). METHODS: A review of all women aged 18 years or younger with a cytologic diagnosis of LSIL or HSIL between 1997 and 2003 was performed. Follow-up cytologic and histologic samples were evaluated. The most significant abnormality was recorded for each patient. Rates of regression, persistence, and progression were calculated. RESULTS: A total of 646 adolescents were identified. Follow-up was available for 477 teenagers with LSIL and for 55 with HSIL. Among adolescents with LSIL, 146 (35%) had negative follow-up. Low-grade abnormalities (atypical squamous cells of undetermined significance, LSIL, and cervical intraepithelial neoplasia grade 1) were seen in 199 (47%), whereas high-grade abnormalities were documented in 77 (18%). After 36 months, 62% had regressed, whereas 31% had progressive dysplasia. For the HSIL cohort, negative follow-up was documented in 12 (21.8%) adolescents, and 15 (27.3%) had low-grade abnormalities, whereas more than one half (50.9%) were found to have a high-grade abnormality. At 36 months, 31% of HSIL subjects had progressed to cervical intraepithelial neoplasia 3. CONCLUSION: Adolescents with LSIL and HSIL cytology are at significant risk for progression to high-grade cervical abnormalities. The rate of development of high-grade cervical abnormalities in adolescents is similar to adults. Adolescents with cytologic abnormalities mandate close follow-up. LEVEL OF EVIDENCE: II-3.

Avaliação da citotoxicidade in vitro de diferentes pastas endodônticas utilizadas em odontopediatria / In vitro cytotoxic effects of different endodontic pastes used in pediatric dentistry

Objetivo: O presente estudo teve como objetivo avaliar o potencial citotóxico de três diferentes pastas endodônticas em cultura celular de osteoblastos humanos. Material e Métodos: As pastas endodônticas Calen PMCC (SSWhite Artigos Dentários LTDA, Rio de Janeiro, Brasil), Feapex (Fórmula e Ação, São Paulo, Brasil) e CTZ (Lenzafarm, Belo Horizonte, Brasil) foram preparadas e eluídas em meio de cultura celular durante 24 horas em estufa a 37°C e 5% de CO2. Foram realizadas quatro diluições distintas desses meios nas concentrações 1: 1, 1: 2, 1: 4 e 1: 8. Culturas de células de Osteoblastos Humanos da linhagem Saos-2 foram expostas a estas diluições durante 24 horas. O controle negativo foi realizado expondo as células ao meio de cultura sem contato com nenhuma pasta endodôntica. A citotoxicidade desses meios foi avaliada utilizando o ensaio MTT e os resultados foram transformados em porcentagens de células viáveis em relação ao grupo controle negativo. A análise estatística foi realizada com nível de significância de 5%, utilizando ANOVA seguido do teste de Tukey. Resultados: A viabilidade celular foi significativamente alterada de acordo com o material testado (p <0,05) e sua concentração (p <0,05). Em todas as concentrações testadas, a pasta Feapex apresentou maior viabilidade celular comparada aos demais materiais (p <0,05). Embora não tenha sido observada diferença estatisticamente significante entre a pasta Calen PMCC e a pasta CTZ nas concentrações de 1: 1 e 1: 2 (p> 0,05), a pasta CTZ apresentou maior citotoxicidade nas concentrações de 1: 4 e 1: 8 (p <0,05 ). De forma geral, a citotoxicidade diminuiu com o aumento da diluição do material. Conclusão: De acordo com os resultados do presente estudo, a pasta endodôntica Feapex parece ser a melhor opção para utilização entre as pastas analisadas, pois apresentou menor citotoxicidade que as pastas Calen PMCC e CTZ

Objective: The aim of the present study was to evaluate the cytotoxic potential of three different endodontic filling materials on human osteoblast cell cultures. Material and Methods: The endodontic pastes Calen PMCC (SSWhite Dental Articles LTDA, Rio de Janeiro, Brazil), Feapex (Formula and Action, São Paulo, Brazil) and CTZ (Lenzafarm, Belo Horizonte, Brazil) were eluted in cell culture medium during 24 hours in an oven at 37°C and 5% CO2. Four distinct dilutions of these media were performed at the concentrations 1:1, 1:2, 1:4 and 1:8. Cell cultures of Saos-2 Human Osteoblast-like were exposed to these dilutions for 24 hours. The negative control group was performed by exposing the cells to culture medium without contact with any endodontic paste. The cytotoxic potential of these media was evaluated using the MTT assay and the results were transformed into viable cell percentages in relation to the negative control group. Statistical analysis was submitted with a significance level of 5%, using univariate Analysis of Variance (ANOVA) followed by Tukey's test. Results: Cell viability was significantly altered according to the material tested (p<0.05) and its concentration (p<0.05). Feapex samples presented higher cell viability than the other materials in all concentrations tested (p<0.05). Although no statistically significant difference was observed between Calen PMCC paste and CTZ paste at concentrations of 1: 1 and 1: 2 (p>0.05), CTZ paste showed a higher cytotoxicity at concentrations of 1: 4 and 1: 8 (p<0.05). In general, cytotoxicity decreases with increasing material dilution. Conclusion: According to the results of the present study, Feapex endodontic paste seems to be the better option for use among the analyzed pastes, since it presented lower cytotoxicity than Calen PMCC and CTZ pastes

Benchmark for Algorithms Segmenting the Left Atrium From 3D CT and MRI Datasets.

Knowledge of left atrial (LA) anatomy is important for atrial fibrillation ablation guidance, fibrosis quantification and biophysical modelling. Segmentation of the LA from Magnetic Resonance Imaging (MRI) and Computed Tomography (CT) images is a complex problem. This manuscript presents a benchmark to evaluate algorithms that address LA segmentation. The datasets, ground truth and evaluation code have been made publicly available through the http://www.cardiacatlas.org website. This manuscript also reports the results of the Left Atrial Segmentation Challenge (LASC) carried out at the STACOM'13 workshop, in conjunction with MICCAI'13. Thirty CT and 30 MRI datasets were provided to participants for segmentation. Each participant segmented the LA including a short part of the LA appendage trunk and proximal sections of the pulmonary veins (PVs). We present results for nine algorithms for CT and eight algorithms for MRI. Results showed that methodologies combining statistical models with region growing approaches were the most appropriate to handle the proposed task. The ground truth and automatic segmentations were standardised to reduce the influence of inconsistently defined regions (e.g., mitral plane, PVs end points, LA appendage). This standardisation framework, which is a contribution of this work, can be used to label and further analyse anatomical regions of the LA. By performing the standardisation directly on the left atrial surface, we can process multiple input data, including meshes exported from different electroanatomical mapping systems.

Atypical squamous cells of undetermined significance in girls and women.

OBJECTIVE: To estimate the outcome of adolescents with atypical squamous cells of undetermined significance (ASC-US) on cytology. METHODS: A review of ASC-US cytology in girls and women aged 10-19 years between 1995 and 1999 was performed. The cytologic and histologic follow-up of each patient was evaluated. The outcome was recorded as the most significant (highest grade) subsequent cervical smear or biopsy. RESULTS: Overall, 535 of 7897 (6.8%) cervical cytologic specimens were reported as ASC-US. The study group consisted of 398 patients for whom pathologic follow-up was available. The mean duration of follow-up was 19 months. Follow-up consisted of repeat cytology in 251 (63%) patients and colposcopy with cervical biopsies and/or endocervical curettage in 147 (37%) of the adolescents. Two hundred fifty-three (64%) adolescents had no pathologic abnormalities on follow-up. Persistent ASC-US was identified in 65 (16%), low-grade squamous intraepithelial lesions/cervical intraepithelial neoplasia (CIN) 1 was found in 44 (11%) and high-grade squamous intraepithelial lesions/CIN 2 or 3 occurred in 36 (9%) of the adolescents. No cases of invasive carcinoma were found. CONCLUSIONS: Among adolescents with ASC-US, the rate of squamous intraepithelial lesions/CIN is similar to that of adults. Although the optimal management of ASC-US in adolescents is unknown, these patients warrant close follow-up. LEVEL OF EVIDENCE: III

ThinPrep evaluation of fluid samples aspirated from cystic ovarian masses.

The cytological evaluation of ovarian cystic fluid using ThinPrep has not been reported. To determine the diagnostic accuracy of ThinPrep cytology in distinguishing between benign and nonbenign ovarian cystic lesions, we examined 65 fluid samples aspirated during intraoperative consultation with subsequent histologic correlation. One ThinPrep slide was prepared from each sample aspirated from surgically removed ovarian cystic masses and reviewed blindly by a panel of three cytopathologists. The parameters used in cytological evaluation were cellularity, cell types, cellular arrangement, and background. Four samples were acellular and excluded from the study. The consensus cytologic diagnoses were compiled for 61 cases which were assigned to one of the following diagnostic categories: negative for malignant cells (40 cases), atypical cytology (13 cases), and suspicious or positive for malignancy (8 cases). Histologic correlation of the cytological benign/negative cases showed that 26/40 (65%) were histologically benign and 14/40 were false-negative (35%, 5 carcinomas and 9 borderline tumors) with 10 of these cases being mucinous tumors. Most false-negative cytologic samples (11/14 or 79%) did not have an epithelial component. Of the 21 cytological nonbenign diagnoses (atypical/suspicious/positive), 15 (71%) were confirmed on histology (10 carcinomas and 5 borderline tumors). However, a nonbenign cytologic diagnosis was rendered in 6 histologically benign cases, including 2 serous cystadenomas, 1 mucinous cystadenoma, 1 serous cystadenofibroma, 1 endometriosis, and 1 corpus luteal cyst. The diagnostic sensitivity by ThinPrep evaluation of ovarian cystic masses is 81% (26/32) for benign and 52% (15/29) for nonbenign lesions. Our results concluded that ThinPrep examination of ovarian cystic fluid is not accurate in distinguishing benign from malignant cysts, given the significant number of false-negative diagnoses. Major contributing factors include sparse cellularity of the fluid samples and mucinous differentiation of the tumors.