RESUMEN
We describe the isolation and cultural characteristics of a Gram-negative bacillus that is very similar to the presumed etiologic agent of cat scratch disease. The organism was isolated from a tibial lesion of a male patient who had been hospitalized for severe necrotizing pancreatitis. The significance of the isolate in this patient remains uncertain.
Asunto(s)
Enfermedad por Rasguño de Gato/microbiología , Bacterias Gramnegativas/aislamiento & purificación , Pancreatitis/complicaciones , Tibia/microbiología , Enfermedad Aguda , Anciano , Biopsia , Enfermedad por Rasguño de Gato/complicaciones , Humanos , Masculino , NecrosisRESUMEN
Six isolates of Propionibacterium acnes recovered from cases of chronic infectious endophthalmitis following extracapsular cataract extraction were tested for antibiotic susceptibility. All isolates were susceptible to penicillin, cefazolin, and vancomycin with a macrobroth dilution method. Minimum bactericidal concentrations testing at 72 h revealed that six of six isolates were killed by < or = 1.0 micrograms of vancomycin/ml, one of six isolates by < or = 1.0 micrograms of penicillin/ml, and zero of six isolates by < or = 1.0 micrograms cefazolin/ml.
Asunto(s)
Antibacterianos/farmacología , Endoftalmitis/microbiología , Propionibacterium acnes/efectos de los fármacos , Cefazolina/farmacología , Enfermedad Crónica , Humanos , Pruebas de Sensibilidad Microbiana , Penicilinas/farmacología , Propionibacterium acnes/aislamiento & purificación , Sensibilidad y Especificidad , Vancomicina/farmacologíaRESUMEN
We established growth curves for Propionibacterium acnes isolates recovered from eyes with chronic postoperative endophthalmitis. The growth curve plotted the average of the duplicate bacterial concentration against time. The generation time for P. acnes calculated from the growth curves was approximately 5.1 hours. The growth of P. acnes is slower than other anaerobic bacteria. This may account for its delayed appearance in culture of ocular specimens. It may also explain treatment failure if the concentration of an antibiotic injected into the vitreous does not remain at an effective level during the critical replicative phase of the organism.
Asunto(s)
Propionibacterium acnes/crecimiento & desarrollo , Extracción de Catarata/efectos adversos , División Celular , Enfermedad Crónica , Endoftalmitis/microbiología , Endoftalmitis/cirugía , Infecciones Bacterianas del Ojo/microbiología , Humanos , Técnicas Microbiológicas , Complicaciones Posoperatorias , Propionibacterium acnes/aislamiento & purificación , Cuerpo Vítreo/microbiologíaRESUMEN
Fifty-three of 54 isolates of fungi were correctly identified with an acridinium ester-labelled probe for Histoplasma capsulatum (Accuprobe; Gen-Probe, San Diego, Calif.). One isolate of Aspergillus niger was incorrectly identified as H. capsulatum. Age of the culture, medium for isolation, and morphologic state did not affect the results.