RESUMEN
OBJECTIVE: To explore the role of microRNA-19a-3p in regulating invasion, metastasis and EMT (epithelial mesenchymal transition) of prostate cancer (PCa) cells, as well as its underlying mechanism. MATERIALS AND METHODS: MicroRNA-19a-3p mimic and negative control plasmid were first constructed. After transfection of microRNA-19a-3p mimic or negative control in DU145 cells, expression levels of microRNA-19a-3p and SOX4 were detected by quantitative Real-time-polymerase chain reaction (qRT-PCR) and Western blot. The regulatory effects of microRNA-19a-3p on migration and invasion of DU145 cells were detected by wound healing assay and transwell assay, respectively. Protein levels of matrix metalloproteinase-2 (MMP2), matrix metalloproteinase-9 (MMP9), N-cadherin, Vimentin, alpha-smooth muscle actin (α-SMA) and E-cadherin in DU145 cells transfected with microRNA-19a-3p mimic or negative control were detected by Western blot. RESULTS: Overexpression of microRNA-19a-3p inhibited protein level of SOX4 in DU145 cells. The migration and invasion of DU145 cells were inhibited after transfection of microRNA-19a-3p mimic. Protein levels of MMP2, MMP9, N-cadherin, Vimentin and α-SMA were downregulated, whereas E-cadherin was upregulated after microRNA-19a-3p overexpression. CONCLUSIONS: MicroRNA-19a-3p inhibits migration, invasion and EMT of PCa cells via inhibiting SOX4.
Asunto(s)
Movimiento Celular , MicroARNs/metabolismo , Neoplasias de la Próstata/metabolismo , Factores de Transcripción SOXC/metabolismo , Línea Celular Tumoral , Transición Epitelial-Mesenquimal , Regulación Neoplásica de la Expresión Génica , Humanos , Masculino , MicroARNs/genética , Invasividad Neoplásica , Metástasis de la Neoplasia , Neoplasias de la Próstata/genética , Neoplasias de la Próstata/patología , Factores de Transcripción SOXC/genética , Transducción de SeñalRESUMEN
OBJECTIVE: To study the chemical constitutents in Peucedanum medium var. gracile distributed in Guangxi. METHOD: Chromatography and spectral analysis were used to isolated and elucidate the constituents. RESULT: Eight compounds were isolated from the root of P. medium var. gracile and elucidated as isoimperatorin, phellopterin, bergapten, oxypeucedanin hydrate, byakangelicin beta-sitosterol, hamaudol and mannitol. CONCLUSION: Seven compounds were obtained from this plant for the first time.