Levels of T-2 toxin and its metabolites, and the occurrence of Fusarium fungi in spring barley in the Czech Republic.

Mycotoxins have been widely studied by many research groups but further multidisciplinary research is needed to better understand and clarify many issues. This study describes the use of high-performance liquid chromatography coupled with ion trap mass spectrometry (HPLC-MS) to measure T-2 toxin and its metabolites, such as HT-2 toxin, neosolaniol (NEO) and diacetoxyscirpenol (DAS), as well as masked glucosylated mycotoxins in Fusarium-infected Czech spring barley. In total, 152 spring barley samples from the 2018 harvest were analyzed by the ELISA screening method for the presence of T-2 toxin. The most contaminated samples (15), which exceeded the recommended maximum level set by the EU for the sum of T-2 and HT-2 toxin in unprocessed cereals (200 µg/kg), were analyzed by HPLC-MS/MS and microbiological testing. Isolated fungi were evaluated microscopically and identified by polymerase chain reaction (PCR) assays. The prevalence of Fusarium species in spring barley across the Czech Republic in 2018 showed a predominance of F. poae (12 barley samples) and F. tricinctum (9 barley samples). Other strains (F. sporotrichioides and F. langsethiae) were present at a lower frequency, in 1 and 2 samples, respectively. The average concentration of T-2 plus HT-2 toxin was 107.7 µg/kg, while NEO and DAS were found in a few samples at values close to their limit of quantification. HT-2 glucoside was identified in all samples.

Influence of school backpack load on plantar foot pressure during walking in 9-11 years old girls.

OBJECTIVE: This study aimed to assess the relative load of the midfoot and the metatarsals of both feet when schoolchildren walked with backpacks of different loads. METHODS: A group of 12 healthy girls (9.9 ± 0.8 years; 33.8 ± 6.7 kg, 1.40 ± 0.10 m) walked barefoot to assess plantar pressures during gait without load (L0%) and with a loaded backpack equal to 10% (L10%) and 20% (L20%) of their body weight. A Footscan® system (RSscan International, Belgium) was used to determine the contact area and relative pressure impulse in the midfoot and metatarsals on the dominant (DL) and non-dominant legs (NL). RESULTS: The effect of load was significant for the contact area of the midfoot for both NL (p = 0.013) and DL (p = 0.001). In the metatarsals, there was significantly greater relative impulse during L10% compared to L0% in the first (p = 0.041) and second (p = 0.050) metatarsals of the DL. Comparing the NL and DL showed significantly greater relative impulse on the DL in the fourth metatarsal during L10% (p = 0.023), greater contact area in the fifth metatarsal during L0% (p = 0.050), and greater impulse in the midfoot during L20% (p = 0.028) on the NL. CONCLUSIONS: The school backpack load influences relative plantar pressure distribution, especially in the midfoot. Further, our findings suggest greater propulsion of the DL and supporting function of the NL.

Gait Variability and Complexity during Single and Dual-Task Walking on Different Surfaces in Outdoor Environment.

Nowadays, gait assessment in the real life environment is gaining more attention. Therefore, it is desirable to know how some factors, such as surfaces (natural, artificial) or dual-tasking, influence real life gait pattern. The aim of this study was to assess gait variability and gait complexity during single and dual-task walking on different surfaces in an outdoor environment. Twenty-nine healthy young adults aged 23.31 ± 2.26 years (18 females, 11 males) walked at their preferred walking speed on three different surfaces (asphalt, cobbles, grass) in single-task and in two dual-task conditions (manual task-carrying a cup filled with water, cognitive task-subtracting the number 7). A triaxial inertial sensor attached to the lower trunk was used to record trunk acceleration during gait. From 15 strides, sample entropy (SampEn) as an indicator of gait complexity and root mean square (RMS) as an indicator of gait variability were computed. The findings demonstrate that in an outdoor environment, the surfaces significantly impacted only gait variability, not complexity, and that the tasks affected both gait variability and complexity in young healthy adults.

Analysis of Whole-Body Coordination Patterning in Successful and Faulty Spikes Using Self-Organising Map-Based Cluster Analysis: A Secondary Analysis.

This study investigated the whole-body coordination patterning in successful and faulty spikes using self-organising map-based cluster analysis. Ten young, elite volleyball players (aged 15.5 ± 0.7 years) performed 60 volleyball spikes in a real-game environment. Adopting the cluster analysis, based on a self-organising map, whole-body coordination patterning was explored between successful and faulty spikes of individual players. The self-organising maps (SOMs) portrayed whole body, lower and upper limb coordination dissimilarities during the jump phase and the ball impact phases between the successful and faulty spikes. The cluster analysis illustrated that the whole body, upper limb and lower limb coordination patterning of each individual's successful spikes were similar to their faulty spikes. Range of motion patterning also demonstrated no differences in kinematics between spike outcomes. Further, the upper limb angular velocity patterning of the players' successful/faulty spikes were similar. The SPM analysis portrayed significant differences between the normalized upper limb angular velocities from 35% to 45% and from 76% to 100% of the spike movement. Although the lower limb angular velocities are vital for achieving higher jumps in volleyball spikes, the results of this study portrayed that the upper limb angular velocities distinguish the differences between successful and faulty spikes among the attackers. This confirms the fact that volleyball coaches should shift their focus toward the upper limb velocity and coordination training for higher success rates in spiking for volleyball attackers.

Postural Control Strategies and Balance-Related Factors in Individuals with Traumatic Transtibial Amputations.

Mechanisms behind compromised balance control in people with transtibial amputation need to be further explored, as currently little is known specifically about postural control strategies in people with traumatic transtibial amputation (tTTA). The aim of this study is to assess automatic and voluntary postural control strategies in individuals with unilateral tTTA compared to those in control subjects and to define the effect of balance-related factors on these strategies. Automatic posture reactions and volitional motion toward given direction using standardized posturographic protocols (NeuroCom) of the Motor Control Test (MCT) and Limits of Stability (LOS) were assessed in eighteen participants with tTTA and eighteen age-matched controls. Compared to the controls, the participants with tTTA bore less weight on the prosthetic leg (p < 0.001) during the MCT and had reduced inclination toward the prosthetic leg (p < 0.001) within the LOS. In the tTTA group, the weight-bearing symmetry and the inclination toward the prosthetic leg (p < 0.05) was positively correlated with prosthesis use duration (p < 0.05). The current study indicates that decreased utilization of the prosthetic leg in tTTAs represents adaptive postural control strategy, but as prosthesis use duration increased, the engagement of the prosthetic leg improved.

Kinematic differences between successful and faulty spikes in young volleyball players.

This study was designated to investigate the kinematical differences between successful and faulty spikes, in order to identify the best strategies leading to better spike performance. Simulating a real-game condition, 13 elite youth attackers performed 6 spikes in the presence of 2 blocks. The kinematic variables of the spike performances were recorded using 6 optoelectronic cameras (Vicon Motion systems, Oxford, UK). The paired sample t-test was used to compare the kinematic variables recorded during the delivery of successful and faulty spikes. Among the successful trials, both the angular velocities of the knees (≈12.4%) and hips (≈13.3%), and the vertical velocity of the centre of mass at take-off (≈6.5%) and arm swing (≈8.2%) were considerably higher during the plant phase. Consequently, the jump (≈4.3%) and spike (≈1.5%) heights, as well as the wrist velocity (≈5.5%), were significantly higher during the jump phase of successful spikes. In successful spike performances, the attackers adopted higher hip and knee angular velocities, combined with efficient arm swings, to produce higher take-off velocities and reach higher jump heights. This approach provides them with the better position regarding the ball and the blockers to find the best path and hit the ball with higher arm velocities.

Acute Effect of Ankle Kinesio and Athletic Taping on Ankle Range of Motion During Various Agility Tests in Athletes With Chronic Ankle Sprain.

BACKGROUND: Taping is a preventive measure commonly used for protecting and strengthening the ankle joint to prevent further musculoskeletal damage. Ankle taping prevents excessive range of motion (ROM) of the ankle joint and allows the improvement of proprioception to adjust balance. Appropriate ankle stability is essential for various activities, such as sprinting, turning, cutting, and jumping, which are associated with agility. AIM: To assess the acute effect of Kinesio taping and athletic taping on the ankle ROM of athletes with chronic ankle sprain during various agility tests that include sprinting, turning, and cutting actions. METHODS: Twenty-five physically active volunteers with chronic ankle sprain performed the Illinois, 5-0-5, 10-m shuttle, hexagon, compass drill, and T agility tests in 3 ankle conditions (nontaped, Kinesio taped, and athletic taped), in random order. Ankle ROM was recorded using the Vicon motion capture system. RESULTS: In comparison with the nontaped ankle condition, in the ankle Kinesio-taping condition, the results showed a significant increase of ankle ROM in the sprinting part of the Illinois, 5-0-5, 10-m shuttle, and T agility tests (P ≤ .01), whereas in the ankle athletic-taping condition, no significant difference was found in ankle ROM during all agility tests. CONCLUSION: In sports that need linear sprinting, Kinesio taping seems to be a suitable intervention for the improvement of sports performance as it provides increased ankle ROM.

Acute Effect of Ankle Kinesio™ Taping on Lower-Limb Biomechanics During Single-Legged Drop Landing.

CONTEXT: Chronic ankle instability is documented to be followed by a recurrence of giving away episodes due to impairments in mechanical support. The application of ankle Kinesiotaping (KT) as a therapeutic intervention has been increasingly raised among athletes and physiotherapists. OBJECTIVES: This study aimed to investigate the impacts of ankle KT on the lower-limb kinematics, kinetics, dynamic balance, and muscle activity of college athletes with chronic ankle instability. DESIGN: A crossover study design. PARTICIPANTS: Twenty-eight college athletes with chronic ankle sprain (11 females and 17 males, 23.46 [2.65] y, 175.36 [11.49] cm, 70.12 [14.11] kg) participated in this study. SETTING: The participants executed 3 single-leg drop landings under nontaped and ankle Kinesio-taped conditions. Ankle, knee, and hip kinematics, kinetics, and dynamic balance status and the lateral gastrocnemius, medial gastrocnemius, tibialis anterior, and peroneus longus muscle activity were recorded and analyzed. RESULTS: The application of ankle KT decreased ankle joint range of motion (P = .039) and angular velocities (P = .044) in the sagittal plane, ground reaction force rate of loading (P = .019), and mediolateral time to stability (P = .035). The lateral gastrocnemius (0.002) and peroneus longus (0.046) activity amplitudes also experienced a significant decrease after initial ground contact when the participants' ankles were taped, while the application of ankle KT resulted in an increase in the peroneus longus (0.014) activity amplitudes before initial ground contact. CONCLUSIONS: Ankle lateral supports provided by KT potentially decreases mechanical stresses applied to the lower limbs, aids in dynamic balance, and lowers calf muscle energy consumption; therefore, it could be offered as a suitable supportive means for acute usage in athletes with chronic ankle instability.

Effect of COL5A1, GDF5, and PPARA Genes on a Movement Screen and Neuromuscular Performance in Adolescent Team Sport Athletes.

Stastny, P, Lehnert, M, De Ste Croix, M, Petr, M, Svoboda, Z, Maixnerova, E, Varekova, R, Botek, M, Petrek, M, Lenka, K, and Cieszczyk, P. Effect of COL5A1, GDF5, and PPARA genes on a movement screen and neuromuscular performance in adolescent team sport athletes. J Strength Cond Res 33(8): 2057-2065, 2019-The risk of injury increases with adolescents' chronological age and may be related to limited muscle function neuromuscular, genetic, and biomechanical factors. The purpose of this study was to determine whether COL5A1, PPARA, and GDF5 genes are associated with muscle functions and stretch-shortening cycle performance in adolescent athletes. One hundred forty-six youth players (14.4 ± 0.2 years) from various team sports (basketball n = 54, soccer n = 50, handball n = 32) underwent a manual test for muscle function, maturity estimation, functional bend test (FBT), passive straight leg raise (SLR) test, leg stiffness test, test of reactive strength index (RSI), and gene sampling for COL5A1, PPARA, and GDF5. The χ test did not show any differences in allele or genotype frequency between participants before and after peak height velocity. Multivariate analysis of variance showed that COL5A1 rs12722 CT heterozygotes had worse score in FBT (p < 0.001), worse score in SLR (p = 0.003), and lower maturity offset (p = 0.029, only in females) than TT homozygotes. Male GDF5 rs143383 GG homozygotes showed better score in SLR than AA and AG genotypes (p = 0.003), and AA and AG genotypes in both sex had greater RSI than GG homozygotes (p = 0.016). The PPARA rs4253778 CC homozygotes had greater RSI than GG and GC genotypes (p = 0.004). The CT genotype in COL5A1 rs12722 is possible predictor of functional movement disruption in the posterior hip muscle chain, causing shortening in FBT and SLR, which includes hamstrings function. CT genotype in COL5A1 rs12722 should be involved in programs targeting hamstring and posterior hip muscle chain.

Comparison of gait kinematics between professional ballet dancers and non-dancers.

OBJECTIVES: The aims of the study were to assess the kinematics of the lower limbs and pelvis during normal walking in professional ballet dancers and to investigate relationships between movements of segments of the lower limbs and pelvis. METHODS: Thirty one professional ballet dancers and twenty eight controls completed five walking trials at their preferred speed. Kinematic data in the basic anatomical planes for ankle, knee, and hip joints as well as for the pelvis were collected with an optoelectronic motion system. RESULTS: The female ballet dancers had in comparison with the controls significantly larger (p < 0.01) knee flexion in the swing phase and hip abduction in the preswing phase. Compared to the control group, the male ballet dancers had significantly larger dorsiflexion in the final stance and the total pelvic tilt range of motion. The number of significant correlations between kinematic parameters was higher in the female ballet dancers. CONCLUSIONS: It can be concluded that specific movement techniques and compensatory strategies used in ballet dance can alter relationships between movements of segments of the lower limbs during normal walking. The relationships between movements in the joints of the lower limbs and pelvis are stronger in women.

The Effects of Nordic Walking and Slope of the Ground on Lower Limb Muscle Activity.

Psurny, M, Svoboda, Z, Janura, M, Kubonova, E, Bizovska, L, Martinez Lemos, RI, and Abrantes, J. The effects of Nordic walking and slope of the ground on lower limb muscle activity. J Strength Cond Res 32(1): 217-222, 2018-Nordic walking (NW) has proven to be a simple and safe mode of exercise that can be used in various types of sport, recreation, and rehabilitation activities. The aim of this study was to assess the effect of Nordic walking and slope of the ground on lower limb muscle activity. The experimental group consisted of 22 healthy men (aged 22.8 ± 1.4 years). The subjects walked on a treadmill at a self-selected speed. Two walking conditions (NW and walking) and 2 ground slopes (level ground and uphill walking at an 8% incline) were used. The surface electromyographic signals of the gastrocnemius lateralis, tibialis anterior, vastus medialis, rectus femoris, biceps femoris and gluteus medius were recorded. Nordic walking resulted in increased activity of some lower limb muscles, particularly during the first half of the stance phase, and decreased muscle activity during the first half of the swing phase. Uphill walking elicited increased muscle activity compared with level walking, particularly during the stance phase and the second half of the swing phase during both walking and NW, and the change was more pronounced during walking. We concluded that NW increased muscle activity in the lower extremities compared with walking, particularly on level ground. Increasing the ground slope enhanced the muscle activity to a much greater extent than NW.

Reliability of Ankle⁻Foot Complex Isokinetic Strength Assessment Using the Isomed 2000 Dynamometer.

For quantifying muscle strength in clinical and research practice, establishing the reliability of measurements, specifically to the procedures used, is essential for credible findings. The objective was to establish the reliability of isokinetic measurement of ankle plantar and dorsal flexors (PF/DF) and invertors and evertors (INV/EV) on an IsoMed 2000 dynamometer. Twenty healthy subjects (10 males, 10 females, mean age: 23.1 ± 3.1 years) completed an isokinetic measurement session. The intraclass correlation coefficient (ICC) and standard error of measurement were assessed for peak torque and work of ankle PF/DF (concentric and eccentric) and INV/EV (concentric) for the preferred and nonpreferred limb. Standardized isokinetic measurements of reciprocal PF/DF and INV/EV muscle actions were associated with ICC ranging from 0.77 to 0.98 for the majority of observed parameters. The exception was work in the eccentric mode in the ankle DF and peak torque in the concentric mode in the ankle INV on the preferred limb, where ICC ranged from 0.64 to 0.71. The IsoMed 2000 isokinetic dynamometer can be reliably employed in future studies for reciprocal ankle PF/DF and INV/EV assessment in healthy adult subjects after implementation of a familiarization session.

Advanced conservative treatment of complete acute rupture of the lateral ankle ligaments: Verifying by stabilometry.

BACKGROUND: The aim of this study was to investigate the result of the specific conservative treatment of acute lateral ankle ligaments rupture and verify the effect of this therapy by stabilometry. METHODS: 17 young athletes were examined after acute lateral ankle sprain (grade III). Diagnosis was based on musculoskeletal ultrasound examinations. Pressure plate evaluated postural stability after conservative treatment at regular intervals during 1year. RESULTS: There were no significant differences in postural stability in double-leg stance between limbs. In single-leg stance, COP confidence ellipse (p=0,011) and COP excursion in sagittal plane (p=0,000) were significantly higher for the injured leg when compared with the uninjured leg only one week after removing the cast. CONCLUSIONS: After conservative treatment of grade III injuries with STABHA, immobilization with full weight bearing on the injured leg for 6 weeks and rehabilitation, stabilometry results showed that none of the patients had impaired postural stability or were at risk of functional ankle instability in the monitored period.

A combination of additives can synergically decrease acrylamide content in gingerbread without compromising sensory quality.

BACKGROUND: The present study tested whether replacement of the leavening agent ammonium carbonate by sodium hydrogen carbonate in combination with calcium cation and acidifying agent will synergically decrease acrylamide (AA) content in gingerbread. RESULTS: The type of leavening agent and the presence of Ca2+ and citric acid accounted for 33.6%, 13.2% and 53.2% of the explained variability of the AA content, respectively (P < 0.01). The AA content in gingerbread produced with (NH4 )2 CO3 alone was 186.5 µg kg-1 . Irrespective of other tested additives, NaHCO3 decreased (P < 0.05) AA content to 42% compared to (NH4 )2 CO3 . Combination of NaHCO3 + CaCl2 + citric acid in dough reduced (P < 0.05) AA content below the limit of detection (25 µg kg-1 ). The AA content in gingerbread (y; µg kg-1 ) decreased with an increasing number of additives used (x) according to the equation y = 158.8 - 47.94x (r2 = 0.42; P < 0.0001). A comprehensive sensory analysis did not indicate any significant deterioration (P > 0.05) in the organoleptic quality of gingerbread produced using calcium cation and citric acid. CONCLUSION: The present study demonstrates that the combination of additives NaHCO3 /Ca2+ /citric acid synergically decreases AA content in gingerbread without compromising the sensory quality. © 2016 Society of Chemical Industry.

The effect of brisk walking on postural stability, bone mineral density, body weight and composition in women over 50 years with a sedentary occupation: a randomized controlled trial.

BACKGROUND: To assess the effect of brisk walking on postural stability, bone mineral density (BMD) and body composition in women over 50 years of age with a sedentary occupation. METHODS: A 10-week walking intervention based on self-regulated brisk walking (BW) to or from work of 30-35 min at least 5 times per week. The research included a total of 104 women (58 women in intervention group). The mean center of pressure (COP) velocity in medial-lateral and anterior-posterior directions, mean total COP velocity with eyes open and closed, BMD of the distal forearm and the calcaneus, body weight, fat mass, and lean body mass were assessed. RESULTS: The BW intervention was completed by 76 % of participants. A significant effect (time × group interaction) was confirmed only in the mean COP velocity in the anterior-posterior direction with eyes closed (F = 7.41, P = 0.008). The effect of BW was not confirmed in BMD, body weight, or body composition. The results indicate that the effect of the intervention is influenced by baseline body mass index in body weight, fat mass and visceral adipose tissue. CONCLUSIONS: BW prevents the deterioration of postural stability with eyes closed, which can have a direct effect on reducing the risk of falls under worse spatial orientation and visibility. The presented intervention model is insufficient for weight loss, changes in BMD, or body composition, and its effect should be assessed during a longer period of time. TRIAL REGISTRATION: German Clinical Trials Register DRKS00007638 , registered March 10, 2015 (retrospectively registered).

Evaluation of Explosive Power Performance in Ski Jumpers and Nordic Combined Competitive Athletes: A 19-Year Study.

Between 1992 and 2010, a total of 334 males participated in this study that assessed the differences and relationships between anthropometric variables and lower limb muscle strength in young and adult ski jumpers (n = 207) and Nordic combined (NC, n = 127) athletes. All athletes completed a maximal vertical jump from an in-run position and a maximal relative isometric force (MRIF) of the knee extensor measurement in a laboratory setting. The body mass index (BMI) in young competitors was lower than in adult groups (NC: p < 0.001; ski jumping [SJ]: p < 0.001). Similarly, the MRIF in both limbs was lower for both disciplines in the groups of young competitors. The vertical jump height (VJH) was lower for young competitors than for adults (NC: p ≤ 0.05; SJ: p < 0.001). When comparing SJ and NC athletes, BMI was lower in SJ athletes. In addition, the adult SJ competitors exhibited greater values of bilateral MRIF (p ≤ 0.05) and VJH (p < 0.01). There was a strong positive correlation in MRIF between the left and right lower limbs (p < 0.001) for all groups of SJ and NC athletes; therefore, it was determined to be sufficient to measure the MRIF on a single limb. Application of the new training methods (e.g., less emphasis on maximum resistance exercises) resulted in improved explosive power in ski jumpers even at lower-body weights. These changes are in accordance with the change in ski jump techniques.

Effect of repeated therapeutic horse riding sessions on the trunk movement of the rider.

OBJECTIVE: To assess the rider's movement during walking the horse in repeated therapeutic horse riding sessions and to determine the relationship between movements of the horse's back and the rider's trunk. METHOD: A total of 12 healthy females (age: 23.3±2.8 years; height: 167.3±4.2 cm; weight: 59.2±5.3 kg) participated in 10 therapeutic horse riding sessions. Two English Thoroughbreds with similar body constitution (aged 19 and 14 years) were used in the experiment. Nine markers were placed on the rider's body and the horse's back, and four video cameras with a 25 Hz frequency were used. Collected data were processed with APAS software. RESULTS: The mediolateral displacements of C7, Th12, and L5 were gradually decreasing in each of the first three sessions. Statistically significant differences (p<0.05) were found between individual sessions in the displacement of C7, Th12 and L5 in the mediolateral and vertical directions as well as in the shoulder and pelvic lateral tilt and rotation. These differences did not show any general tendencies regarding the riders' increasing experiences with riding. The relationships between the displacement of C7, Th12, and L5 and the sacral tuber on the horse's back in the vertical direction were statistically significant (p<0.01) during all sessions. CONCLUSIONS: The displacement of C7, Th12 and L5 in the mediolateral direction decreased during riding sessions. A significant relationship was found between the vertical movement of the horse's back and the rider's trunk. These relationships differed between the horses.

Ski-jumpers' performance in a laboratory setting: a 29-year longitudinal study.

This study assesses the longitudinal changes in anthropometric and motor parameters of ski jumpers. Male ski jumpers (n = 329) at various competitive levels participated in this study. These competitors were divided into two groups by age (18 years and younger, and over 18 years), and then divided into seven even intervals within those groups. Basic anthropometric parameters, maximal relative isometric knee extensor force, reaction time, knee extension time, and vertical jump height were measured. The conditions, instruments, and systems of measurement were consistent throughout the study. A reduced body mass index (BMI) in the adult jumpers was significant (P < .01) in the first three (1982-1993) and in the last three (1998-2010) intervals. Adults had an increase of maximal relative isometric knee extensor force (P < .01) in the last three intervals (1998-2010). They had greater maximal relative isometric knee extensor force (with exception of the first interval, 1982-1985) and vertical jump height than younger competitors (P < .01). Both young and adult jumpers exhibited the increase of strength and power in the lower limbs and a decrease in BMI during the whole observed period.

Does the Dumbbell-Carrying Position Change the Muscle Activity in Split Squats and Walking Lunges?

The forward walking lunge (WL) and split squat (SSq) are similar exercises that have differences in the eccentric phase, and both can be performed in the ipsilateral or contralateral carrying conditions. This study aimed to determine the effects of dumbbell-carrying position on the kinematics and electromyographic (EMG) amplitudes of the gluteus medius (Gmed), vastus medialis (VM), vastus lateralis (VL), and biceps femoris during WLs and SSqs. The resistance-trained (RT) and the non-resistance-trained (NT) groups (both n = 14) performed ipsilateral WLs, contralateral WLs, ipsilateral SSqs, and contralateral SSqs in a randomized order in a simulated training session. The EMG amplitude, expressed as a percentage of the maximal voluntary isometric contraction (%MVIC), and the kinematics, expressed as the range of motion (ROM) of the hip and knee, were measured during 5 repetition maximum for both legs. The repeated measure analyses of variance showed significant differences between the RT and NT groups. The NT group showed a smaller knee flexion ROM (p < 0.001, η = 0.36) during both types of WLs, whereas the RT group showed a higher eccentric Gmed amplitude (p < 0.001, η = 0.46) during all exercises and a higher eccentric VL amplitude (p < 0.001, η = 0.63) during contralateral WLs. Further differences were found between contralateral and ipsilateral WLs in both the RT (p < 0.001, η = 0.69) and NT groups (p < 0.001, η = 0.80), and contralateral WLs resulted in higher eccentric Gmed amplitudes. Contralateral WLs highly activated the Gmed (90% MVIC); therefore, this exercise can increase the Gmed maximal strength. The ipsilateral loading condition did not increase the Gmed or VM activity in the RT or NT group.

Utilization of oil extracted from spent coffee grounds for sustainable production of polyhydroxyalkanoates.

Spent coffee grounds (SCG), an important waste product of the coffee industry, contain approximately 15 wt% of coffee oil. The aim of this work was to investigate the utilization of oil extracted from SCG as a substrate for the production of poly(3-hydroxybutyrate) (PHB) by Cupriavidus necator H16. When compared to other waste/inexpensive oils, the utilization of coffee oil resulted in the highest biomass as well as PHB yields. Since the correlation of PHB yields and the acid value of oil indicated a positive effect of the presence of free fatty acids in oil on PHB production (correlation coefficient R (2) = 0.9058), superior properties of coffee oil can be probably attributed to the high content of free fatty acids which can be simply utilized by the bacteria culture. Employing the fed-batch mode of cultivation, the PHB yields, the PHB content in biomass, the volumetric productivity, and the Y P/S yield coefficient reached 49.4 g/l, 89.1 wt%, 1.33 g/(l h), and 0.82 g per g of oil, respectively. SCG are annually produced worldwide in extensive amounts and are disposed as solid waste. Hence, the utilization of coffee oil extracted from SCG is likely to improve significantly the economic aspects of PHB production. Moreover, since oil extraction decreased the calorific value of SCG by only about 9 % (from 19.61 to 17.86 MJ/kg), residual SCG after oil extraction can be used as fuel to at least partially cover heat and energy demands of fermentation, which should even improve the economic feasibility of the process.