1.
Biosci Biotechnol Biochem
; 88(1): 70-73, 2023 Dec 19.
Artículo
en Inglés
| MEDLINE
| ID: mdl-37793878
RESUMEN
We have devised a method for the direct screening of efficient short hairpin (sh)RNA molecules in human cells, eliminating the need for the time-consuming process of cloning in Escherichia coli. Our screening suggested that single mismatches to shRNAs can significantly alter their activity.