Dracaena sanderiana endophytic bacteria interactions: Effect of endophyte inoculation on bisphenol A removal.

Bisphenol A (BPA) is one of the most abundant endocrine-disrupting compounds which is found in the aquatic environment. However, actual knowledge regarding the effect of plant-bacteria interactions on enhancing BPA removal is still lacking. In the present study, Dracaena sanderiana endophytic bacteria interactions were investigated to evaluate the effect of bacterial inoculation on BPA removal under hydroponic conditions. Two plant growth-promoting (PGP) bacterial strains, Bacillus thuringiensis and Pantoea dispersa, which have high BPA tolerance and can utilize BPA for growth, were used as plant inocula. P. dispersa-inoculated plants showed the highest BPA removal efficiency at 92.32 ±â€¯1.23% compared to other inoculated and non-inoculated plants. This was due to a higher population of the endophytic inoculum within the plant tissues which resulted in maintained levels of indole-3-acetic acid (IAA) for the plant's physiological needs and lower levels of reactive oxygen species (ROS). In contrast, B. thuringiensis-inoculated plants had a lower BPA removal efficiency. However, individual B. thuringiensis possessed a significantly higher BPA removal efficiency compared to P. dispersa. This study provides convincing evidence that not all PGP endophytic bacteria-plant interactions could improve the BPA removal efficiency. Different inocula and inoculation times should be investigated before using plant inoculation to enhance phytoremediation.

Air-borne xylene degradation by Bougainvillea buttiana and the role of epiphytic bacteria in the degradation.

The efficiency of xylene removal from contaminated air by thirteen perennial plants was studied. The results showed that Bougainvillea buttiana had the highest xylene removal efficiency. Different parts of B. buttiana such as stems, epicuticular waxes, and plant stomata (including microorganism-associated plant leaves) can uptake xylene 53.1±1.9%, 32.3±0.9, and 14.6±0.0%, respectively. Metabolite products found in treated plants may result from stress or defense compounds triggered by exposure to xylene. Moreover, possible degradation products in B. buttiana stems were analyzed after treatment with xylene at 100 ppm. Various metabolites in B. buttiana stems such as 2,6-dimethoxyphenol, 4-hydroxy-3,5-dimethoxy benzoic acid, 1-isopropyl-4-methylbenzene, p-tolualdehyde, 2,5-dimethoxy-4-methylbenzaldehyde, 2,4-dihydroxy-2,5-dimethyl-3(2H)-furanone, 3-methyl-2-butenal, dihydroxy acetone, propanedial, and many organic acids are related to the xylene degradation pathway. In addition, microorganism-associated B. buttiana leaves especially Enterobacter cloacae LSRC11, Staphylococcus sp. A1 and Pseudomonas aeruginosa enhanced the plant resulting in quicker xylene removal.

Phytodegradation of Ethanolamines by Cyperus alternifolius: Effect of Molecular Size.

Our screening of plants showed that Cyperus alternifolius (Umbrella papyrus) had the highest efficiency removal in real wastewater containing monoethanolamine-higher than Echinodorus cordifolius (Creeping Burrhead), Thalia geniculata (Alligator Flag), Acorus calamus (Sweet Flag), and Dracaena sanderiana (Lucky Bamboo). Therefore, this research studied the degradation of monoethanolamine (MEA), diethanolamine (DEA), and triethanolamine (TEA) by C. alternifolius. Plants could degrade TEA into DEA, then into MEA, and then further into acetic acid. The accumulation of ethanolamines was found mainly in plant stems, which had the highest biomass. This demonstrated that the molecular size is closely related to a diffusion coefficient that affects the removal rate through plant bodies. A smaller molecular weight-MEA (MW = 61.08 g mol(-1))-was taken up the fastest, followed by DEA (MW = 105.14 g mol(-1)) and TEA (MW = 149.19 g mol(-1)), the highest molecular weight. The plants' toxicity when exposed to ethanolamines elucidated that MEA had the highest toxicity, followed by DEA and TEA. In addition, the application of C. alternifolius in monoethanolamine-contaminated wastewater revealed that plant could completely uptake MEA at day 5 from an initial MEA concentration of 18 mM. The result indicated that C. alternifolius has the potential to remove ethanolamines and can be applied to ethanolamine-contaminated wastewater.

Phosphorus removal from domestic wastewater by Nelumbo nucifera Gaertn. and Cyperus alternifolius L.

Domestic wastewater is a source of phosphorus contamination that causes eutrophication when it contaminates aquatic environments. Nelumbo nucifera Gaertn. and Cyperus alternifolius L. were applied for phosphorus removal from domestic wastewater. From the study, phosphorus in domestic wastewater was removed from the initial concentration of 1.038 ± 0.001 mgL(-1) to 0.094 ± 0.001 and 0.048 ± 0.004 mgL(-1) by N. nucifera and C. alternifolius, respectively, within 5 days. In addition, total Kjeldahl nitrogen (TKN) and chemical oxygen demand (COD) also decreased when wetland systems were applied the same as treatment with conventional method (chemical + activated sludge process). However, the plant removed TDS better than the conventional method. During 5 cycles of exposure, the two plants still survived and were healthy. The weight of plants increased after the experiment from 4060 ± 0.05 g to 4820 ± 0.17 g of N. nucifera, and from 4000 ± 0.00 g to 4600 ± 0.14 g of C. alternifolius. Phosphorus content also increased in both plants after the experiment. However, in the wetland system, phosphorus was removed mainly by the soil, followed by the plants, and then microorganisms. The domain group in the microbial community of both wetland systems was Pseudomas sp.

A constructed wetland model for synthetic reactive dye wastewater treatment by narrow-leaved cattails (Typha angustifolia Linn.).

Textile wastewater is contaminated by reactive dye causing unattractive levels of wastewater color, high pH and high salt content when discharged into public water systems. Decolorization of textile wastewater by plant, phytoremediation, is an alternative, sustainable method which is suitable for long term operation. Narrow-leaved cattails are one species of wetland plant with efficiency for decolorizing and remediating textile wastewater. In addition, chemical oxygen demand (COD) can be lowered and dye residue can be removed. The plant also showed a good salt tolerance even after being exposed to a salt solution for 15 days. The narrow-leaved cattails were set up in a constructed wetland model with a vertical flow system operating from bottom to top for synthetic reactive dye wastewater (SRDW) removal. Narrow-leaved cattails could achieve the removal of SRDW at approximately 0.8 g(SRDW) m(-2) day(-1). Decolorization of SRDW by this plant was approximately 60%. The advantage of this method is that it is suitable for textile wastewater management and improvement of wetland. These plants could lower COD, remove dye, sodium and total dissolved solids (TDS) whereas other biological and chemical methods could not remove TDS and dye in the same time. These results suggested that the spongy cell structure of this plant has the ability to absorb large amounts of water and nutrients. Physico-chemical analysis revealed increasing amounts of sulfur, silicon, iron and calcium in the plant leafs and roots after exposure to wastewater. Proteins or amide groups in the plant might help in textile dye removal. Regarding decolorization, this plant accumulates dye in the intercellular space and still grows in this SRDW condition. Hence, it can be noted here that narrow-leaved cattails are efficient for textile dye wastewater treatment.

Calcium acetate-induced reduction of cadmium accumulation in Oryza sativa: Expression of auto-inhibited calcium-ATPase and cadmium transporters.

Calcium (Ca) signalling has an essential role in regulating plant responses to various abiotic stresses. This study applied Ca in various forms (Ca acetate and CaCl2 ) and concentrations to reduce cadmium (Cd) concentration in rice and propose a possible mechanism through which Ca acts to control the Cd concentration in rice. The results showed that supplementation of Cd-contaminated soil with Ca acetate reduced the Cd concentration in rice after exposure for 7 days in both hydroponic and soil conditions. The possible involvement of the auto-inhibited Ca2+ -ATPase gene (ACA) might act to control the primary signal of the Cd stress response. The messages from ACA3 and ACA13 tended to up-regulate the low-affinity cation transporter (OsLCT1) and down-regulate Cd uptake and the Cd translocation transporter, including the genes, natural resistance-associated macrophage protein 5 (Nramp5) and Zn/Cd-transporting ATPase 2 (HMA2), which resulted in a reduction in the Cd concentration in rice. After cultivation for 120 days, the application of Ca acetate into Cd-contaminated soil inhibited Cd uptake of rice. Increasing the Ca acetate concentration in the soil lowered the Cd concentration in rice shoots and grains. Moreover, Ca acetate maintained rice productivity and quality whereas both aspects decreased under Cd stress.

Synthetic reactive dye wastewater treatment by narrow-leaved cattails (Typha angustifolia Linn.): effects of dye, salinity and metals.

Narrow-leaved cattails were studied in synthetic reactive dye wastewater (SRDW) under caustic conditions. The effects of the toxic dye were expressed in terms of relative plant growth rate and the appearance of symptoms such as necrosis, and chronic or acute wilting. The dye toxicity was 25.33 mg l(-1) which was close to approximate the concentration of dye residue from the textile effluent in the public stream. The system pH and % color removal were decreased, indicating that narrow-leaved cattail can treat wastewater. The average system pH decreased from 9 to 7. The maximum color removal was approximately 60% when cultured under soil conditions. The SEM image of narrow-leaved cattail root after treatment with SRDW indicated that the root cortex was damaged and the crystalline sodium salts deposited in the root cells which caused evaporation and transpiration decreased in SRDW. The salinity under caustic conditions also affects the growth of the plants. The maximum sodium removal was approximately 44% and was found in the SRDW under soil conditions within 14 days. A small amount of sodium could enhance the relative growth rate. However, the sodium removal of the plants was limited after the third week of treatment. It should be noted that narrow-leaved cattails are known to avoid the textile dye and salt stress conditions during SRDW treatment through special mechanisms such as salt accumulation in the roots or shedding of older leaves. In addition, elements such as silicon, calcium and iron in plants might help the plant to detoxify by forming complexes with dye molecules.

Application of 'waste' metal hydroxide sludge for adsorption of azo reactive dyes.

The capacity and mechanism of metal hydroxide sludge in removing azo reactive dyes from aqueous solution was investigated with different parameters, such as charge amount of dyes, system pH, adsorbent particle size, and adsorbent dosage. The three anionic dyes used were CI Reactive Red 2, CI Reactive Red 120, and CI Reactive Red 141, increasing in number of sulfonic groups, respectively. Only 0.2% (w/v) of powdered sludge (<75microm) achieved color removal from 30 mg l(-1) reactive dye solutions within 5 min without pH adjustment. The larger the charge amount of the dyes, the greater the adsorption (>90%) on the metal hydroxide sludge. The system pH played a significant role in the adsorption on metal hydroxides and formation of dye-metal complexes. The optimum system pH for dye adsorption was 8-9 which was close to the pH(zpc) of the sludge while the precipitation of dye-metal complexes occurred at system pH 2. The maximum adsorption capacity (Q degrees ) of the sludge for the reactive dyes was 48-62 mg dye g(-1) adsorbent. The Langmuir and Freundlich models showed that the higher charged dyes had a higher affinity of adsorption. The smaller particle size and the greater amount of adsorbent showed the faster process, due to an increase in surface area of adsorbent. Desorption studies elucidated that metal hydroxide sludge had a tendency for ion exchange adsorption of sulfonated azo reactive dyes. Leaching data showed that the treated water was nontoxic at a system pH above 5 or a solution pH above 2.

Evaluation of metal hydroxide sludge for reactive dye adsorption in a fixed-bed column system.

The capacity and performance of small-scale column, containing coarse particles of metal hydroxide sludge, were evaluated using 30mgl(-1) dye solutions of C.I. Reactive Red 141. The studied bed depths were 2.5-20cm and the studied flow rates were 1.1, 2.2 and 3.3mlmin(-1)cm(-2). At the breakthrough point of 0.1C(t)/C(0), the breakthrough volume was increased with increasing bed depth or decreasing flow rate, due to an increase in empty bed contact time (EBCT). The data followed the bed depth service time model, and the adsorption capacity was 24-26mgcm(-3) or 27-29mgdyesg(-1) adsorbent. The minimum bed depths should be higher 1.02, 2.04 and 2.59cm with flow rates of 1.1, 2.2 and 3.3mlmin(-1)cm(-2), respectively, while the ratio of bed depth to diameter should not be higher than 6. With EBCT above 5min, the usage rate of metal hydroxide sludge was 1.3gl(-1). Using the bed depth of 5cm and the flow rate of 0.55mlmin(-1)cm(-2), 87% of dominant colour, 78% of COD, and 99% of SS could be removed from the textile wastewater, and the leachate of toxic heavy metals was under the standard limitations.

Phytoremediation of bisphenol A and total dissolved solids by the mangrove plant, Bruguiera gymnorhiza.

Bruguiera gymnorhiza, an evergreen mangrove tree, is tolerant of bisphenol A (BPA) and has potential BPA removal capability. BPA is highly toxic to plants at high concentrations, wherein they exhibit damaged symptoms such as chlorosis, necrosis, and wilting. The LD50 of BPA toxicity for this plant was statistically estimated to be 39.97 mg L(-1). B. gymnorhiza can reduce COD from 15408 +/- 246 to 49 +/- 30 mg L(-1) by (approximately 99% reduction of the initial value) and can reduce content to levels below the industrial effluent standard of Thailand (<120 mg L(-1)) within 48 days. This plant can completely remove BPA from the solution within 51 days of treatment. Polysaccharides and organic acids were found in the solution and were caused by plant response to the toxicity of BPA. In addition, B. gymnorrhiza can also reduce total dissolved solids (TDS) and salinity in real wastewater. Therefore, B. gymnorrhiza has potential for removal of BPA and TDS in contaminated in wastewater.

Phytoremediaton of diethylene glycol contaminated wastewater by Echinodorus cordifolius.

Diethylene glycol (DEG) is one of several diols used as a raw material in the production of plasticizers and polyester resins. It has been associated with a number of mass poisonings in several countries. Conventional methods of remediation of DEG contaminated wastewaters are still not very effective. This paper presents an alternative method for remediation of DEG-contaminated waters using the plant Echinodorus cordifolius. The effects of DEG on E. cordifolius were studied along with the plant's efficiency at treating DEG-contaminated wastewater in a constructed wetland. We found that DEG was toxic to the plants with an LD50 of 6238 mg L(-1). The plants exhibited decreased water uptake and showed wilting, chlorosis and necrosis. SEM images showed injury to the cortex tissue. In the constructed wetland, E. cordifolius plants were able to remove and degrade DEG from wastewater, decreasing the pH from 12 to 6.8 and the COD and TDS by approximately 98% and 67%, respectively, in 7 days, while accumulating Ca in the cells.

Diethylene glycol removal by Echinodorus cordifolius (L.): the role of plant-microbe interactions.

This work presents the use of the plant Echinodorus cordifolius for remediating diethylene glycol (DEG) contaminated waters. The potential of this plant for treating DEG wastewater in a remediation system was observed. We found that E. cordifolius was able to remove DEG from wastewater, decrease the pH to neutral and remove approximately 95% of the chemical oxygen demand within 12 days. The plants can grow well in DEG wastewater, as indicated by their root and leaf biomass, which was found to be statistically similar to control. Wilting, chlorosis and necrosis were observed in DEG-treated plants, but the relative water content was not significantly different between control and treated plants, suggesting that the plants were able to take up and tolerate DEG present in the wastewater. Plant roots changed to black colour during experimental period. The fluorescence in situ hybridisation and bacterial enrichment confirmed that 4.30 × 10(5) cells/g of sulphate reducing bacteria and 9.30 × 10(8) cells/g of acid-producing bacteria were found associated with the plant roots. Furthermore, volatile fatty acids were found in non-sterile soil treatments, indicating that soil microorganisms are associated with DEG remediation. These results demonstrated that plants and bacteria have the ability to form a relationship to remove the organic contaminant DEG.

Bisphenol A removal by the Dracaena plant and the role of plant-associating bacteria.

Dracaena sanderiana and Dracaena fragrans plants, as representatives of native, tropical, evergreen plants with fibrous root systems, were evaluated for bisphenol A (BPA) tolerance and uptake capability. D. sanderiana demonstrated significantly higher BPA removal capability than D. fragrans. Therefore, it was chosen for further study. D. sanderiana tolerated BPA toxicity levels up to 80 microM, while higher BPA concentrations damaged the plant. In the sterile hydroponic system with an initial BPA concentration of 20 microM, the plant could uptake approximately 50% of the BPA. The plant's ability to translocate BPA was confirmed by the detection of BPA that accumulated at the roots and stems, but not at the leaves of the plant. Upon BPA exposure, the D. sanderiana secreted extracellular plant mucilage as a protective barrier to the toxic compound. In the non-sterile treatment, the BPA dissipation was contributed not only by the D. sanderiana plant, but also by the co-existing microbes. The BPA reached 85% of the initial concentration at 20 microM. Among the six plant-associating bacterial isolates, Bacillus cereus strain BPW4 and Enterobacter sp. strain BPW5 colonized the D. sanderiana root surface and facilitated BPA dissipation in the hydroponic treatment system. In addition, the success of the BPA treatment in the hazardous waste landfill leachate demonstrated the potential application of D. sanderiana plant in the phytoremediation of BPA contaminated wastewater or industrial leachate.

Nickel adsorption by sodium polyacrylate-grafted activated carbon.

A novel sodium polyacrylate grafted activated carbon was produced by using gamma radiation to increase the number of functional groups on the surface. After irradiation the capacity for nickel adsorption was studied and found to have increased from 44.1 to 55.7 mg g(-1). X-ray absorption spectroscopy showed that the adsorbed nickel on activated carbon and irradiation-grafted activated carbon was coordinated with 6 oxygen atoms at 2.04-2.06 A. It is proposed that this grafting technique could be applied to other adsorbents to increase the efficiency of metal adsorption.