Atypical spindle cell lipomatous tumour presenting as a large retroperitoneal mass - a case report and review of the literature.

Atypical spindle cell lipomatous tumour (ASCLT) is a benign neoplasm that presents a variable proportion of atypical spindle and adipocytic cells, frequently expressing CD34, and embedded in myxoid or collagenous matrix. An important feature is a constant lack of either MDM2 or CDK4 amplification. It typically arises in the extremities. The retroperitoneum is a rare site of involvement. We report a case of a retroperitoneal ASCLT in a 62-year-old male. A differential diagnosis of ASCLT from the other mesenchymal, spindle-cell, and lipomatous tumours is crucial for optimal treatment and significantly influences the prognosis. A diagnosis should be warranted by the immunohistochemistry and molecular findings.

Development and Validation of Multi-Residue Method for Drugs Analysis in Human Feces by Liquid Chromatography-Tandem Mass Spectrometry.

The use of veterinary drugs in animal production is a common practice to secure animal and human health. However, residues of administrated drugs could be present in animal food products. Levels of drugs in food of animal origin are regulated within the European Union. In recent years, residues have been detected not only in food, but also in the environmental elements such as water or soil, meaning that humans are involuntarily exposed to these substances. This article presents a multiclass method for the analysis of various therapeutic groups of pharmaceuticals in human feces. Pharmaceuticals are extracted from feces with an acid extraction solvent, and after filtration the extract was analyzed by HPLC-MS/MS. A limit of detection of 10 ng/g was achieved for 9 pharmaceuticals, with linearity over 0.99 and repeatability and reproducibility lower than 20%. The method was satisfactorily applied in 25 feces samples of individuals that had declared not to be under medical treatment for the last two months. Results indicate the presence of six different compounds at concentration between 10 and 456 ng/g. This preliminary study showed the involuntary exposure of human gut microbiota to active substances such as pharmaceuticals.

Capacity of underwater optical wireless communication systems over salinity-induced oceanic turbulence channels with ISI.

Point-to-point underwater optical wireless communication (UOWC) links are mainly impaired by scattering due to impurities and turbidity in the open water, resulting in a significant inter-symbol interference (ISI) that limits seriously both channel capacity and the maximum practical information rate. This paper conducts, for the first time, the channel capacity analysis of UOWC systems in the presence of ISI and salinity-induced oceanic turbulence when the undersea optical channel is accurately modeled by linear discrete-time filtering of the input symbols. In this way, novel upper and lower bounds on channel capacity and mutual information are developed for non-uniform on-off keying (OOK) modulation when different constraints are imposed on the channel input. The results show that the capacity-achieving distribution, which is computed through numerical optimization, is discrete and depends on the optical signal-to-noise-ratio (SNR). Moreover, a non-uniform input distribution significantly improves the channel capacity of such systems affected by ISI and oceanic turbulence, especially at low optical SNR. Monte Carlo techniques are employed to test the developed bounds for different undersea optical channels with one, two and three casual ISI coefficients.

Interferon gamma, TGF-ß1 and RANTES expression in upper airway samples from SARS-CoV-2 infected patients.

Upper respiratory tract is the primary site of SARS-CoV-2 replication. Releasing of pro and anti-inflammatory mediators plays an important role in the immunopathogenesis of Coronavirus Disease 2019 (COVID-19). The aim of this study was to evaluate the early inflammatory response in upper airway by measuring of IFN-γ, TGF-ß1 and RANTES at mRNA level. Forty five SARS-CoV-2 infected patients were enrolled, whose were divided in two groups: asymptomatic and symptomatic. Twenty healthy persons, SARS-CoV-2 negative were included as controls. Higher IFN-γ expression was detected in SARS-CoV-2 infected patients in comparison with controls (p = 0.0393). IFN-γ expression was increased in symptomatic patients (p = 0.0405). TGF-ß1 and RANTES expressions were lower in SARS-CoV-2 infected patients than controls (p < 0.0001; p = 0.0011, respectively). A significant correlation between IFN-γ and TGF-ß1 was observed in SARS-CoV-2 asymptomatic patients (r = +0.61, p = 0.0014). The findings suggest that imbalance between IFN-γ and TGF-ß1 expression could be an impact in clinical expression of SARS-CoV-2 infection.

Impact of angular pointing error on BER performance of underwater optical wireless links.

Even in clear ocean water, underwater optical wireless communication (UOWC) is impaired not only by absorption and scattering, but also by oceanic turbulence and dynamic pointing errors which result in a fading channel, degrading the bit error rate (BER) performance. In this paper, for the first time, we quantify analytically the trade-off between geometric loss and misalignment in underwater scattering channels. A novel geometric loss model is developed which is used to compute the average BER in the presence of absorption and scattering over salinity-induced oceanic turbulence channels. Our findings suggest that UOWC systems are less sensitive to angular pointing errors due to jitter since scattering is able to alleviate such a fading effect at the expense of a higher attenuation due to geometric spread. Monte Carlo simulation results are further included to verify the developed BER expression which is valid over a wide range of signal-to-noise-ratio (SNR). Finally, the impact of inter-symbol interference (ISI) is also quantified by measuring the optical power penalty.

Secure communication for FSO links in the presence of eavesdropper with generic location and orientation.

When the beam waist at the receiver is significantly larger than the receiver size, free-space optical (FSO) links may be vulnerable to some optical tapping risks at the physical layer. In this paper, we conduct a new framework for the analysis of the secrecy performance in terms of the secrecy outage probability (SOP) of FSO systems affected by gamma-gamma (GG) turbulence-induced fading channels with pointing errors. As a key feature, we evaluate the SOP in the presence of an external eavesdropper with generic location and orientation. For that reason, a new misalignment error model is proposed to consider a non-orthogonal optical beam with respect to the photodetector plane at the eavesdropper's receiver, where the effective area is determined by a rotated ellipse. New approximate and asymptotic solutions at high signal-to-noise-ratio (SNR) for the secrecy performance are obtained in closed-form, which are verified by exact Monte Carlo simulations. By using the developed expressions, we analyze in greater detail some effects such as the SNR of the eavesdropper's channel, the normalized beamwidth at the receiver-side, and the location and orientation of the eavesdropper on the secrecy performance for different turbulence conditions.

Antimicrobial activity of apitoxin from Apis mellifera in Salmonella enterica strains isolated from poultry and its effects on motility, biofilm formation and gene expression.

Salmonella is a major global food-borne pathogen. One of the main concerns related to Salmonella and other food-borne pathogens is their capacity to acquire antimicrobial resistance and produce biofilms. Due to the increased resistance to common antimicrobials used to treat livestock animals and human infections, the discovery of new antimicrobial substances is one of the main challenges in microbiological research. An additional challenge is the development of new methods and substances to inhibit and destruct biofilms. We determined the antimicrobial and antibiofilm activities of apitoxin in 16 Salmonella strains isolated from poultry. In addition, the effect of apitoxin on Salmonella motility and the expression of biofilm- and virulence-related genes was evaluated. The minimum inhibitory concentrations (MIC) of apitoxin ranged from 1,024-256 µg/mL, with 512 µg/mL being the most common. Sub-inhibitory concentrations of apitoxin significantly reduced biofilm formation in 14 of the 16 Salmonella strains tested, with significant increases in motility. MIC concentrations of apitoxin destroyed the pre-formed biofilm by 27.66-68.22% (47.00% ±â€¯10.91). The expression of biofilm- and virulence-related genes and small RNAs was differentially regulated according to the strain and the presence of apitoxin. The transcription of the small RNAs dsrA and csrB, related to antimicrobial resistance, was upregulated in the presence of apitoxin. We suggest that apitoxin is a potential antimicrobial substance that could be used in combination with other substances to develop new drugs and sanitizers against food-borne pathogens.

Tracing Recombinant Bovine Somatotropin Ab(Use) Through Gene Expression in Blood, Hair Follicles, and Milk Somatic Cells: A Matrix Comparison.

The use of recombinant bovine somatotropin (rbST) in dairy cattle is forbidden in the European Union. Due to the very low circulating concentration of rbST in treated animals, its direct detection is still a challenge. Therefore, the use of indirect methods to detect the ab(use) of rbST in dairy cattle appears as a good alternative. In the past few years, gene expression demonstrated its utility in screening the use of illicit substances in both humans and animals. In this study, a comparison of three types of matrices (milk somatic cells, blood, and hair follicles) was carried out to evaluate their potential use for routine control of rbST using 15 gene-expression profiles. A total of six rbST-treated cows and three control cows were included in the study. A subcutaneous injection containing 500 mg of rbST was administered to the treated group. Samples of the three matrices were collected before rbST administration, and at three and nine days after treatment. The quality of RNA extracted was higher in the blood and hair-follicle samples than in the milk somatic cells. In the three matrices, there were significant differences in the expression of some genes, with milk somatic cells and blood presenting the the best matrices. On this note, the cyclin D1 (CCND1), interleukin 1 beta (IL-1ß), tumor necrosis factor (TNF), and insulin-like growth factor 1 receptor (IGF-1R) genes showed potential as biomarkers of rbST treatment. Therefore, blood, somatic cells, and follicle hair should be considered as promising sources of RNA, and can be used in gene-expression assays to routinely control the illicit use of rbST.

Salmonella and Campylobacter biofilm formation: a comparative assessment from farm to fork.

It takes several steps to bring food from the farm to the fork (dining table), and contamination with food-borne pathogens can occur at any point in the process. Campylobacter spp. and Salmonella spp. are the main microorganisms responsible for foodborne disease in the EU. These two pathogens are able to persist throughout the food supply chain thanks to their ability to form biofilms. Owing to the high prevalence of Salmonella and especially of Campylobacter in the food supply chain and the huge efforts of food authorities to reduce these levels, it is of great importance to fully understand their mechanisms of persistence. Diverse studies have evaluated the biofilm-forming capacity of foodborne pathogens isolated at different steps of food production. Nonetheless, the principal obstacle of these studies is to reproduce the real conditions that microorganisms encounter in the food supply chain. While there are a wide number of Salmonella biofilm studies, information on Campylobacter biofilms is still limited. A comparison between the two microorganisms could help to develop new research in the field of Campylobacter biofilms. Therefore, this review evaluates relevant work in the field of Salmonella and Campylobacter biofilms and the applicability of the data obtained from these studies to real working conditions. © 2018 Society of Chemical Industry.

Posttransfusion Increase of Hematocrit per se Does Not Improve Circulatory Oxygen Delivery due to Increased Blood Viscosity.

BACKGROUND: Blood transfusion is used to treat acute anemia with the goal of increasing blood oxygen-carrying capacity as determined by hematocrit (Hct) and oxygen delivery (DO2). However, increasing Hct also increases blood viscosity, which may thus lower DO2 if the arterial circulation is a rigid hydraulic system as the resistance to blood flow will increase. The net effect of transfusion on DO2 in this system can be analyzed by using the relationship between Hct and systemic blood viscosity of circulating blood at the posttransfusion Hct to calculate DO2 and comparing this value with pretransfusion DO2. We hypothesized that increasing Hct would increase DO2 and tested our hypothesis by mathematically modeling DO2 in the circulation. METHODS: Calculations were made assuming a normal cardiac output (5 L/min) with degrees of anemia ranging from 5% to 80% Hct deficit. We analyzed the effects of transfusing 0.5 or more units of 300 cc of packed red blood cells (PRBCs) at an Hct of 65% and calculated microcirculatory DO2 after accounting for increased blood viscosity and assuming no change in blood pressure. Our model accounts for O2 diffusion out of the circulation before blood arriving to the nutritional circulation and for changes in blood flow velocity. The immediate posttransfusion DO2 was also compared with DO2 after the transient increase in volume due to transfusion has subsided. RESULTS: Blood transfusion of up to 3 units of PRBCs increased DO2 when Hct (or hemoglobin) was 60% lower than normal, but did not increase DO2 when administered before this threshold. CONCLUSIONS: After accounting for the effect of increasing blood viscosity on blood flow owing to increasing Hct, we found in a mathematical simulation of DO2 that transfusion of up to 3 units of PRBCs does not increase DO2, unless anemia is the result of an Hct deficit greater than 60%. Observations that transfusions occasionally result in clinical improvement suggest that other mechanisms possibly related to increased blood viscosity may compensate for the absence of increase in DO2.

Identification and quantification of 12 pharmaceuticals in water collected from milking parlors: Food safety implications.

The introduction of drug residues into the food chain and their presence in drinking water has been recently investigated. The aim of this work was to monitor the presence of 19 active drugs in water samples collected from milking parlors of dairy farms located in Galicia (northwest Spain), one of the main Spanish milking areas. Overall, 65% of the samples tested positive for at least one of the compounds analyzed. A total of 12 drugs were measured, with concentrations ranging between 17 and 3,941 ng/L. Considering that a mixture of compounds may contribute to the overall effect of each compound and might increase or reduce its toxicity, it should be noted that 29% of the samples tested contained more than one pharmaceutical. To date, the effects of the continuous consumption of these mixtures of drugs in water or milk are unknown; however, antimicrobials may affect the human gut microbiota or have toxic effects in sensitive individuals.

Novel approximation of misalignment fading modeled by Beckmann distribution on free-space optical links.

A novel accurate and useful approximation of the well-known Beckmann distribution is presented here, which is used to model generalized pointing errors in the context of free-space optical (FSO) communication systems. We derive an approximate closed-form probability density function (PDF) for the composite gamma-gamma (GG) atmospheric turbulence with the pointing error model using the proposed approximation of the Beckmann distribution, which is valid for most practical terrestrial FSO links. This approximation takes into account the effect of the beam width, different jitters for the elevation and the horizontal displacement and the simultaneous effect of nonzero boresight errors for each axis at the receiver plane. Additionally, the proposed approximation allows us to delimit two different FSO scenarios. The first of them is when atmospheric turbulence is the dominant effect in relation to generalized pointing errors, and the second one when generalized pointing error is the dominant effect in relation to atmospheric turbulence. The second FSO scenario has not been studied in-depth by the research community. Moreover, the accuracy of the method is measured both visually and quantitatively using curve-fitting metrics. Simulation results are further included to confirm the analytical results.

Impact of nonzero boresight pointing error on ergodic capacity of MIMO FSO communication systems.

A thorough investigation of the impact of nonzero boresight pointing errors on the ergodic capacity of multiple-input/multiple-output (MIMO) free-space optical (FSO) systems with equal gain combining (EGC) reception under different turbulence models, which are modeled as statistically independent, but not necessarily identically distributed (i.n.i.d.) is addressed in this paper. Novel closed-form asymptotic expressions at high signal-to-noise ratio (SNR) for the ergodic capacity of MIMO FSO systems are derived when different geometric arrangements of the receive apertures at the receiver are considered in order to reduce the effect of nonzero inherent boresight displacement, which is inevitably present when more than one receive aperture is considered. As a result, the asymptotic ergodic capacity of MIMO FSO systems is evaluated over log-normal (LN), gamma-gamma (GG) and exponentiated Weibull (EW) atmospheric turbulence in order to study different turbulence conditions, different sizes of receive apertures as well as different aperture averaging conditions. It is concluded that the use of single-input/multiple-output (SIMO) and MIMO techniques can significantly increase the ergodic capacity respect to the direct path link when the inherent boresight displacement takes small values, i.e. when the spacing among receive apertures is not too big. The effect of nonzero additional boresight errors, which is due to the thermal expansion of the building, is evaluated in multiple-input/single-output (MISO) and single-input/single-output (SISO) FSO systems. Simulation results are further included to confirm the analytical results.

Ergodic capacity analysis for DF strategies in cooperative FSO systems.

This paper focuses on the ergodic capacity analysis in the context of cooperative free-space optical (FSO) systems when the line of sight is available. Novel asymptotic closed-form expressions for the ergodic capacity corresponding to two different decode-and-forward (DF) strategies are obtained for a cooperative FSO communication system. Here, the atmospheric turbulence is modeled by a gamma-gamma distribution of parameters α and ß which allows to study a wide range of turbulence conditions (moderate-to-strong) as well as the effect of the misalignment with zero boresight. It is demonstrated that cooperative communications are able to achieve not only a better performance in terms of the error rate performance as well as outage probability than direct transmission, but also in terms of the channel capacity in the context of FSO systems without much increase in hardware. In this way, a 3-way FSO communication setup is considered, in which the cooperative protocol can be applied to achieve a greater ergodic capacity compared to a direct transmission. It can be concluded that a greater and robust capacity strongly dependent on the relay location is achieved compared to a direct transmission without cooperative communication when line of sight is available. Here, the line of sight is taken into account in order to achieve a significant robustness under different turbulence conditions and more severe pointing errors regardless of the relay location. Simulation results are further demonstrated to confirm the accuracy and usefulness of the derived results.

Novel space-time trellis codes for free-space optical communications using transmit laser selection.

In this paper, the deployment of novel space-time trellis codes (STTCs) with transmit laser selection (TLS) for free-space optical (FSO) communication systems using intensity modulation and direct detection (IM/DD) over atmospheric turbulence and misalignment fading channels is presented. Combining TLS and STTC with rate 1 bit/(s · Hz), a new code design criterion based on the use of the largest order statistics is here proposed for multiple-input/single-output (MISO) FSO systems in order to improve the diversity order gain by properly chosing the transmit lasers out of the available L lasers. Based on a pairwise error probability (PEP) analysis, closed-form asymptotic bit error-rate (BER) expressions in the range from low to high signal-to-noise ratio (SNR) are derived when the irradiance of the transmitted optical beam is susceptible to moderate-to-strong turbulence conditions, following a gamma-gamma (GG) distribution, and pointing error effects, following a misalignment fading model where the effect of beam width, detector size and jitter variance is considered. Obtained results show diversity orders of 2L and 3L when simple two-state and four-state STTCs are considered, respectively. Simulation results are further demonstrated to confirm the analytical results.

On the capacity of MISO FSO systems over gamma-gamma and misalignment fading channels.

In this work, the ergodic capacity performance for multiple-input/single-output (MISO) free-space optical (FSO) communications system with equal gain combining (EGC) reception is analyzed over gamma-gamma and misalignment fading channels, which are modeled as statistically independent, but not necessarily identically distributed (i.n.i.d.). Novel and analytical closed-form ergodic capacity expression is obtained in terms of H-Fox function by using the well-known inequality between arithmetic and geometric mean of positive random variables (RV) in order to obtain an approximate closed-form expression of the distribution of the sum of M gamma-gamma with pointing errors variates. In addition, we present an asymptotic ergodic capacity expression at high signal-to-noise ratio (SNR) for the ergodic capacity of MISO FSO systems. It can be concluded that the use of MISO technique can significantly reduce the effect of the atmospheric turbulence as well as pointing errors and, hence, provide significant capacity gain over the direct path link (DL). The impact of pointing errors on the MISO FSO system is also analyzed, which only depends on the number of laser sources and pointing error parameters. Moreover, it can be also concluded that the ergodic capacity performance is dramatically reduced as a consequence of the severity of pointing error effects. Simulation results are further demonstrated to confirm the analytical results.

Impact of relay placement on diversity order in adaptive selective DF relay-assisted FSO communications.

The impact of relay placement on diversity order in adaptive selective decode-and-forward (DF) cooperative strategies is here investigated in the context of free-space optical (FSO) communications over atmospheric turbulence channels with pointing errors when line of sight is available. The irradiance of the transmitted optical beam here considered is susceptible to moderate-to-strong turbulence conditions, following a gamma-gamma (GG) distribution together with a misalignment fading model where the effect of beam width, detector size and jitter variance is considered. Novel closed-form approximate bit error-rate (BER) expressions are obtained for a cooperative FSO communication setup with N relays, assuming that these relays are located in an area similar to an annulus around source or destination node. An analytical expression is here found that determines the best selection criterion based on the knowledge of the channel state information (CSI) of source-relay or relay-destination links in order to significantly increase the diversity order corresponding to the cooperative strategy under study. It is concluded that the highest diversity order is achieved when the relation ß(SR(min)) > ß(SD) + ß(R(min)D) is satisfied, wherein ß(SR(min)), ß(R(min)D) and ß(SD) are parameters corresponding to the atmospheric turbulence conditions of source-relay and relay-destination link with the greatest scintillation index, and source-destination link, respectively.

Transmit alternate laser selection with time diversity for FSO communications.

In this paper, a new transmit alternate laser selection (TALS) scheme for FSO communication systems using intensity modulation and direct detection (IM/DD) over atmospheric turbulence and misalignment fading channels is presented when limited time diversity is available in the turbulent channel. Assuming channel state information (CSI) at the transmitter and receiver and a time diversity order (TDO) limited, we propose the transmit diversity technique based on the rotating selection of TDO out of the available L lasers corresponding to the optical paths with greater values of scintillation. Implementing repetition coding with blocks of TDO information bits, each information bit will be retransmitted TDO times using the TDO largest order statistics in an alternating way. Closed-form asymptotic bit error-rate (BER) expressions are derived when the irradiance of the transmitted optical beam is susceptible to moderate-to-strong turbulence conditions, following a gamma-gamma (GG) distribution, and pointing error effects, following a misalignment fading model where the effect of beam width, detector size and jitter variance is considered. Fully exploiting the potential time-diversity TDO available in the turbulent channel, a significant diversity gain is achieved, providing a diversity order of (2L + 1 - TDO)TDO/2.

Adaptive selective relaying in cooperative free-space optical systems over atmospheric turbulence and misalignment fading channels.

In this paper, a novel adaptive cooperative protocol with multiple relays using detect-and-forward (DF) over atmospheric turbulence channels with pointing errors is proposed. The adaptive DF cooperative protocol here analyzed is based on the selection of the optical path, source-destination or different source-relay links, with a greater value of fading gain or irradiance, maintaining a high diversity order. Closed-form asymptotic bit error-rate (BER) expressions are obtained for a cooperative free-space optical (FSO) communication system with Nr relays, when the irradiance of the transmitted optical beam is susceptible to either a wide range of turbulence conditions, following a gamma-gamma distribution of parameters α and ß, or pointing errors, following a misalignment fading model where the effect of beam width, detector size and jitter variance is considered. A greater robustness for different link distances and pointing errors is corroborated by the obtained results if compared with similar cooperative schemes or equivalent multiple-input multiple-output (MIMO) systems. Simulation results are further demonstrated to confirm the accuracy and usefulness of the derived results.