RESUMEN
ÏRS138, a bacteriophage of the family Siphoviridae that lyses Ralstonia solanacearum, was isolated. The genomic DNA of ÏRS138 was 41,941 bp long with a GC content of 65.1 % and contained 56 putative open reading frames. The ÏRS138 genome could be divided into three regions based on similarities to other genomes: (1) a region containing genes encoding a putative transcriptional regulator and an integrase, similar to the prophage genes in Ralstonia solanacearum K60-1; (2) a region encoding proteins related to structural modules and virion morphogenesis, similar to genes in the Pseudomonas phages of the family Siphoviridae; and (3) a region highly similar to the genomes of other Ralstonia solanacearum strains.
Asunto(s)
Bacteriófagos/genética , Bacteriófagos/aislamiento & purificación , Genoma Viral , Ralstonia solanacearum/virología , Siphoviridae/genética , Siphoviridae/aislamiento & purificación , Composición de Base , ADN Viral/química , ADN Viral/genética , Orden Génico , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Análisis de Secuencia de ADN , Homología de SecuenciaRESUMEN
Filamentous bacteriophage RS611 (ÏRS611), which infects the phytopathogen Ralstonia solanacearum, had a circular single-stranded DNA genome that was characterized as an Ff-type phage belonging to the family Inoviridae. The ÏRS611 genome was composed of 6386 bases with a G + C content of 62.1 % and contained 11 putative open reading frames. The ÏRS611 genome showed high similarity to those of Ralstonia phages RSS0 and RSS1. However, approximately 900-nucleotide deletions were found in the region corresponding to open reading frames 10 and 11 of ÏRSS0 and ÏRSS1.
Asunto(s)
Virus ADN/genética , ADN Viral/química , ADN Viral/genética , Genoma Viral , Inoviridae/genética , Inovirus/genética , Ralstonia solanacearum/virología , Composición de Base , Virus ADN/aislamiento & purificación , ADN Circular/genética , Inoviridae/clasificación , Inoviridae/aislamiento & purificación , Inovirus/clasificación , Inovirus/aislamiento & purificación , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Análisis de Secuencia de ADN , Eliminación de Secuencia , Homología de Secuencia , SinteníaRESUMEN
A filamentous bacteriophage (Ï), ÏRS603, which is infectious to the phytopathogen Ralstonia solanacearum was isolated. ÏRS603 was found to have a circular single-stranded DNA genome composed of 7679 nucleotides and to contain 13 putative open reading frames (ORFs). The ÏRS603 genome showed strong similarity with those of Ralstonia phages ÏRSM1 and ÏRSM3, as reported by Askora et al. The ÏRS603 genome had no ORFs corresponding to ORFs 2, 3, 13 and 14 (integrase) of ÏRSM3. ÏRS603 had an ORF that was homologous to other Ralstonia phages ÏRSS0 and ÏRSS1; however, ÏRSM1 and ÏRSM3 did not.