Response of soil microbial activities and ammonia oxidation potential to environmental factors in a typical antimony mining area.

Mining, smelting and tailing deposition activities can cause metal(loid) contamination in surrounding soils, threatening ecosystems and human health. Microbial indicators are sensitive to environmental factors and have a crucial role in soil ecological risk assessment. Xikuangshan, the largest active antimony (Sb) mine in the world, was taken as the research area. The soil properties, metal(loid) contents and microbial characteristics were investigated and their internal response relationships were explored by multivariate statistical analysis. The assessment of the single pollution index and Nemerow synthetic pollution index (PN) showed that the soils were mainly polluted by Sb, followed by Cd and As, in which sampling site S1 had a slight metal(loid) pollution and the other sampling sites suffered from severe synthetic metal(loid) pollution. The microbial characteristics were dissimilar among sampling points at different locations from the mining area according to hierarchical cluster analysis. The correlation analysis indicated that fluorescein diacetate hydrolase, acid phosphatase, soil basal respiration and microbial biomass carbon were negatively correlated with PN, indicating their sensitivity to combined metal(loid) contamination; that dehydrogenase was positively correlated with pH; and that urease, potential ammonia oxidation and abundance of ammonia-oxidizing bacteria and archaea were correlated with N (nitrogen) contents. However, ß-glucosidase activity had no significant correlations with physicochemical properties and metal(loid) contents. Principal components analysis suggested bioavailable Sb and pH were the dominant factors of soil environment in Xikuangshan Sb mining area. Our results can provide a theoretical basis for ecological risk assessment of contaminated soil.

Behavior of Sb and As in the hydrogeochemistry of adjacent karst underground river systems and the responses of such systems to mining activities.

Through the investigation of Qinglong mining area and adjacent karst underground river system, mining activities and water-rock interactions are found to control the hydrogeochemical evolution of karst underground water. Along the flow direction of the karst underground river, the hydro-chemical type is converted from HCO3-Ca type to SO4-Ca type. The concentrations of Sb and As also gradually decrease. Using PHREEQC to calculate the SI shows that: in the karst underground river system, both gypsum and fluorite are unsaturated, indicating a high degree of water-rock interaction. LogPCO2 is negatively correlated with pH, indicating that the karst underground river systems are both open systems. The dissolution of carbonate minerals and the alternate adsorption of ions are the main water-rock interactions that lead to the rapid decline of Sb and As concentrations. This research also applies principal component analysis to identify the types of pollution in adjacent karst underground river systems. The results show that the LongBaiwei underground river was mainly affected by coal mining activities, and Fe was more prominent; the ShuiYa underground river was more significantly affected by the leachate from the antimony tailings yard. This study provides a scientific basis for the evolution of the water environment as well as strategies for pollution prevention and control in typical karst underground river systems owing to the influence of mining activities.