Evaluation of the marginal and internal gaps of partially crystallized versus fully crystallized zirconia-reinforced lithium silicate CAD-CAM crowns: An in vitro comparison of the silicone replica technique, direct view, and 3-dimensional superimposition analysis.

STATEMENT OF PROBLEM: The crystallization process of lithium disilicate crowns has been reported to cause dimensional change, but whether the fit of chairside computer-aided design and computer-aided manufactured (CAD-CAM) zirconia-reinforced lithium silicate crowns is affected is unclear. PURPOSE: The purpose of this in vitro study was to evaluate with a 3-dimensional superimposition analysis technique the marginal and internal adaptation of fully crystallized versus partially crystallized zirconia-reinforced lithium silicate ceramic CAD-CAM fabricated crowns. Additionally, the silicone replica technique and direct viewing of marginal gap values were compared with the 3-dimensional superimposition analysis technique. MATERIAL AND METHODS: The marginal and internal adaptation of a fully crystallized zirconia-reinforced lithium silicate (CELTRA DUO) were compared with those of a partially crystallized zirconia-reinforced lithium silicate (VITA SUPRINITY) after crystallization. Sixteen crowns (n=8) were fabricated with a chairside CAD-CAM system. The crowns and die and crown assembly were scanned with an optical scanner for the 3-dimensional superimposition analysis. Four hundred sixty-eight measurements were made for each crown, 78 in each 2-dimensional section. Marginal discrepancy was measured by using the direct viewing technique. The internal adaptation of the shoulder area, axial space, and occlusal space was measured by using the silicone replica technique. Both gap values were compared with the 3-dimensional superimposition analysis results by using the independent t test. The 2-way ANOVA was used to detect the effect of each variable (group and site) (α=.05). RESULTS: The VITA SUPRINITY crowns showed statistically higher marginal discrepancy values than the CELTRA DUO crowns in both 3-dimensional superimposition analysis and the direct viewing method, and the lingual aspect recorded the highest marginal discrepancy mean value when compared with other aspects. The 3-dimensional superimposition analysis and the direct viewing method were statistically similar (P=.076). The VITA SUPRINTY crowns showed higher internal gap mean values than the CELTRA DUO crowns in both 3-dimensional superimposition analysis and silicone replica techniques. The occlusal space recorded the highest mean value in both groups. Assessment by 3-dimensional superimposition analysis and silicone replica techniques showed statistical difference in internal gap values (P=.04). CONCLUSIONS: CELTRA DUO showed better precision fit values than VITA SUPRINITY. Three-dimensional superimposition analysis is a reliable method of evaluating marginal and internal adaptation.

Influence of speed sintering on the fit and fracture strength of 3-unit monolithic zirconia fixed partial dentures.

STATEMENT OF PROBLEM: Speed sintering has been introduced to enable single-visit monolithic zirconia prostheses. However, the fit and fracture load of zirconia 3-unit monolithic fixed partial dentures (FPDs) after speed sintering are unknown. PURPOSE: The purpose of this in vitro study was to test the properties of zirconia 3-unit monolithic FPDs after speed sintering and to compare the properties with conventional sintering. MATERIAL AND METHODS: A calibrated operator digitized an in vitro model with a complete coverage preparation of a maxillary right second premolar and second molar (n=12) by using the CEREC AC Omnicam (Dentsply Sirona) scanner. Twelve zirconia FPDs were designed (CEREC SW 4.1.1), and for each data set (n=12), 1 FPD was designed and milled 4 times (MCXL Premium; CEREC Zirconia; Dentsply Sirona), resulting in 4 identical monolithic FPDs (N=48). The FPDs were divided into 2 groups according to the sintering procedure (n=24): speed sintering (group S) by using the SpeedFire (Dentsply Sirona) and the conventional sintering (group C) by using the inFire HTC speed (Dentsply Sirona). All the FPDs were glazed by using glaze-spray and fired according to the sintering group. The SpeedFire (Dentsply Sirona) was used for group S, and the VACUMAT 6000M (VITA Zahnfabrik) was used for group C. The fit of the FPDs was evaluated with the replica technique by using polyvinyl siloxane and analyzed according to the measurement areas: marginal gap, chamfer area, axial wall, and occlusal area. Subsequently, groups S and C were further subdivided, and 12 specimens per group underwent artificial aging by thermomechanical loading in a mastication machine (50 N for 1.2×106 times at a frequency of 1.7 Hz and a thermal change in distilled water from 5 °C to 50 °C every 120 seconds), resulting in additional subgroups: group SA and group CA. For all the FPDs (groups S, C, SA, and CA), a fracture load measurement was conducted. The Kolmogorov-Smirnov test was used to examine the values of the fit and fracture load for normal distribution. The Mann-Whitney U test for the fit and a 2-way ANOVA for the fracture load were used to detect the differences among the groups (α=.05). RESULTS: Group S showed a better marginal (P=.018) and occlusal (P<.001) fit than group C. For the fracture load values, no significant difference was found because of the sintering procedure (P=.070) or the interaction of the sintering procedure and artificial aging (P=.484). Artificial aging showed an impact (P=.024) with significantly lower values after aging. CONCLUSIONS: Speed-sintered FPDs had equal and better values for the fit and fracture load than conventional sintering.

3 D printed replica of the human temporal bone intended for teaching gross anatomy.

The anatomy of the human temporal bone is complex and, therefore, poses unique challenges for students. Furthermore, temporal bones are frequently damaged from handling in educational settings due to their inherent fragility. This report details the production of a durable physical replica of the adult human temporal bone, manufactured using 3D printing technology. The physical replica was printed from a highly accurate virtual 3D model generated from CT scans of an isolated temporal bone. Both the virtual and physical 3D models accurately reproduced the surface anatomy of the temporal bone. Therefore, virtual and physical 3D models of the temporal bone can be used for educational purposes in order to supplant the use of damaged or otherwise fragile human temporal bones.

Evaluation of the marginal fit of CAD-CAM zirconia copings: Comparison of 2D and 3D measurement methods.

PURPOSE: The purpose of this in vitro study was to compare the marginal fit of zirconia copings by using 2 different measurement methods: a triple optical scan method and a silicone replica method. MATERIAL AND METHODS: Sixty zirconia copings fabricated by computer-aided design and computer-aided manufacture (CAD-CAM) systems were studied for the marginal fit. For the replica method, the thickness of the light-body silicone layer of the discrepancy was assessed using light microscopy and image analysis software. The triple-scan optical method was performed to obtain a digital three-dimensional map of the marginal fit and analyzed marginal fit measurement values. For each method, the reliability of the measurement was tested, and a nonparametric analysis was then performed to compare the marginal fit values as measured by the 2 evaluation methods (α=.05). RESULTS: Intraclass correlation coefficients and repeatability coefficients revealed good repeatability for both of the evaluation methods. However, the triple-scan method produced a smaller marginal fit than the replica method (P<.001) for the entire group studied. CONCLUSIONS: Although both methods showed good repeatability, the triple-scan method was more reliable.

Marginal and internal fit of pressed lithium disilicate inlays fabricated with milling, 3D printing, and conventional technologies.

STATEMENT OF PROBLEM: The subtractive and additive computer-aided design and computer-aided manufacturing (CAD-CAM) of lithium disilicate partial coverage restorations is poorly documented. PURPOSE: The purpose of this in vitro study was to compare the marginal and internal fit accuracy of lithium disilicate glass-ceramic inlays fabricated with conventional, milled, and 3-dimensional (3D) printed wax patterns. MATERIAL AND METHODS: A dentoform mandibular first molar was prepared for a mesio-occlusal ceramic inlay. Five groups of 15 inlays were obtained through conventional impression and manual wax pattern (group CICW); conventional impression, laboratory scanning of the stone die, CAD-CAM milled wax blanks (group CIDW) or 3D printed wax patterns (group CI3DW); and scanning of the master preparation with intraoral scanner and CAD-CAM milled (group DSDW) or 3D printed wax patterns (group DS3DW). The same design was used to produce the wax patterns in the last 4 groups. The replica technique was used to measure marginal and internal adaptation by using stereomicroscopy. Mixed-model ANOVA was used to assess differences according to the groups and discrepancy location (α=.05). RESULTS: Group DSDW showed the smallest marginal discrepancy (24.3 µm) compared with those of groups CICW (45.1 µm), CIDW (33.7 µm), CI3DW (39.8 µm), and DS3DW (39.7 µm) (P<.001). No statistically significant differences were detected among groups CICW, CIDW, CI3DW, and DS3DW relative to the marginal discrepancy. The internal discrepancy was significantly larger than the marginal discrepancy within all groups (P<.001). CONCLUSIONS: Lithium disilicate glass-ceramic inlays produced from digital scans and subtractive milling of wax patterns resulted in better marginal and internal fit accuracy than either conventional impression/fabrication or additive 3D manufacturing. Three-dimensional printed wax patterns yielded fit values similar to those of the conventionally waxed inlays.

Architecture of the rat nephron-arterial network: analysis with micro-computed tomography.

Among solid organs, the kidney's vascular network stands out, because each nephron has two distinct capillary structures in series and because tubuloglomerular feedback, one of the mechanisms responsible for blood flow autoregulation, is specific to renal tubules. Tubuloglomerular feedback and the myogenic mechanism, acting jointly, autoregulate single-nephron blood flow. Each generates a self-sustained periodic oscillation and an oscillating electrical signal that propagates upstream along arterioles. Similar electrical signals from other nephrons interact, allowing nephron synchronization. Experimental measurements show synchronization over fields of a few nephrons; simulations based on a simplified network structure that could obscure complex interactions predict more widespread synchronization. To permit more realistic simulations, we made a cast of blood vessels in a rat kidney, performed micro-computed tomography at 2.5-µm resolution, and recorded three-dimensional coordinates of arteries, afferent arterioles, and glomeruli. Nonterminal branches of arcuate arteries form treelike structures requiring two to six bifurcations to reach terminal branches at the tree tops. Terminal arterial structures were either paired branches at the tops of the arterial trees, from which 52.6% of all afferent arterioles originated, or unpaired arteries not at the tree tops, yielding the other 22.9%; the other 24.5% originated directly from nonterminal arteries. Afferent arterioles near the corticomedullary boundary were longer than those farther away, suggesting that juxtamedullary nephrons have longer afferent arterioles. The distance separating origins of pairs of afferent arterioles varied randomly. The results suggest an irregular-network tree structure with vascular nodes, where arteriolar activity and local blood pressure interact.

Variability in coronary artery anatomy affects consistency of cardiac damage after myocardial infarction in mice.

Low reliability and reproducibility in heart failure models are well established. The purpose of the present study is to explore factors that affect model consistency of myocardial infarction (MI) in mice. MI was induced by left coronary artery (LCA) ligation. The coronary artery was casted with resin and visualized with fluorescent imaging ex vivo. LCA characteristics and MI size were analyzed individually in each animal, and MI size was correlated with left ventricular (LV) function by echocardiography. Coronary anatomy varies widely in mice, posing challenges for surgical ligation and resulting in inconsistent MI size postligation. The length of coronary arterial trunk, level of bifurcation, number of branches, and territory supplied by these branches are unique in each animal. When the main LCA trunk is ligated, this results in a large MI, but when a single branch is ligated, MI size is variable due to differing levels of LCA ligation and area supplied by the branches. During the ligation procedure, nearly 40% of LCAs are not grossly visible to the surgeon. In these situations, the surgeon blindly sutures a wider and deeper area of tissue in an attempt to catch the LCA. Paradoxically, these situations have greater odds of resulting in smaller MIs. In conclusion, variation in MI size and LV function after LCA ligation in mice is difficult to avoid. Anatomic diversity of the LCA in mice leads to inconsistency in MI size and functional parameters, and this is independent of potential technical modifications made by the operator.NEW & NOTEWORTHY In the present study, we demonstrate that left coronary artery diversity in mice is one of the primary causes of variable myocardial infarction size and cardiac functional parameters in the left coronary artery ligation model. Recognition of anatomic diversity is essential to improve reliability and reproducibility in heart failure research.

Impact of Yttrium-90 Microsphere Density, Flow Dynamics, and Administration Technique on Spatial Distribution: Analysis Using an In Vitro Model.

PURPOSE: To investigate material density, flow, and viscosity effects on microsphere distribution within an in vitro model designed to simulate hepatic arteries. MATERIALS AND METHODS: A vascular flow model was used to compare distribution of glass and resin surrogates in a clinically derived flow range (60-120 mL/min). Blood-mimicking fluid (BMF) composed of glycerol and water (20%-50% vol/vol) was used to simulate a range of blood viscosities. Microsphere distribution was quantified gravimetrically, and injectate solution was dyed to enable quantification by UV spectrophotometry. Microsphere injection rate (5-30 mL/min) and the influence of contrast agent dilution of injection solution (0%-60% vol/vol) were also investigated. RESULTS: No significant differences in behavior were observed between the glass and resin surrogate materials under any tested flow conditions (P = .182; n = 144 injections). Microspheres tend to align more consistently with the saline injection solution (r2 = 0.5712; n = 144) compared with total BMF flow distribution (r2 = 0.0104; n = 144). The most predictable injectate distribution (ie, greatest alignment with BMF flow, < 5% variation) was demonstrated with > 10-mL/min injection rates of pure saline solution, although < 20% variation with glass microsphere distribution was observed with injection solution containing as much as 30% contrast medium when injected at > 20 mL/min. CONCLUSIONS: Glass and resin yttrium-90 surrogates demonstrated similar distribution in a range of clinically relevant flow conditions, suggesting that microsphere density does not have a significant influence on microsphere distribution. Injection parameters that enhanced the mixing of the spheres with the BMF resulted in the most predictable distribution.

Simulated Wound Assessment Using Digital Planimetry versus Three-Dimensional Cameras: Implications for Clinical Assessment.

BACKGROUND: Clinical management of wounds can benefit from objective measures of response to treatment. Wound surface area and volume are objective measures of wound healing. Using a synthetic wound model, we compare the accuracy and reproducibility of 2 commercially available 3-dimensional (3D) cameras against planimetry and water displacement. METHODS: Twelve ulcers of various sizes and colors were reproduced in modeling clay and cured. Five naive observers used digital planimetry, water displacement, Eykona camera (Fuel 3D, UK), and Silhouette camera (ARANZ, New Zealand) to measure the wounds. RESULTS: When compared with traditional planimetry, wound surface area measurement with Eykona and Silhouette tended to underestimate wounds by 1.7% and 3.7%, respectively. Spearman correlation coefficients were 0.94 (Eykona) and 0.92 (Silhouette). Intraclass correlations for planimetry and the 2 cameras were all 1. Eykona and Silhouette tended to underestimate wound volumes when compared with water displacement by 58% and 23%, respectively. Spearman correlation coefficients were 0.92 (Eykona) and 0.72 (Silhouette). Intraclass correlations for water displacement and the two cameras were all 1. DISCUSSION: Serial accurate objective area measurements are feasible as part of ongoing clinical assessment of wounds. 3D cameras are reliable but have not shown superior accuracy to manual planimetry, and financial concerns and IT integration may limit general clinical usage. Volume measurements of wounds are practicable as part of clinical care.

Electrically conductive synthetic vocal fold replicas for voice production research.

A method of fabricating electrically conductive synthetic vocal fold replicas and monitoring their vibration via resistance measurement is presented. Normally non-conductive silicone replicas were coated with conductive graphite and subjected to long-term vibration tests. Synchronized resistance and imaging data using hemilarynx and full larynx configurations showed an inverse correlation between replica contact area and resistance during vibration, similar to clinical electroglottography (EGG) used to estimate vocal fold contact area. This method has potential for long-term replica vibration monitoring and studying basic physical relationships between resistance and contact area in vocal folds and vocal fold replicas.

Evaluation of Bond Strength, Marginal Integrity, and Fracture Strength of Bulk- vs Incrementally-filled Restorations.

PURPOSE: This study evaluated the effect of application technique and preparation size on the fracture strength (FS), microtensile bond strength (µTBS) and marginal integrity (MI) of direct resin composite restorations. MATERIALS AND METHODS: Conservative (5 × 2 × 2 mm) or extended (5 × 4 × 2 mm) preparations below the cementoenamel junction were performed in 140 human maxillary premolars (n = 70 per group). After adhesive application (XP Bond), half of each group was restored with the bulk technique (one 4-mm increment of Surefill SDR Flow plus one 1-mm horizontal capping layer of TPH3 [Spectrum TPH3 resin composite]) and half incrementally (TPH3 in three horizontal incremental layers, 1.5 to 2 mm each), all using a metal matrix band. After storage (24 h at 37°C), the proximal surfaces of each tooth were polished with Sof-Lex disks. For FS measurement, 60 restorations were mounted in a universal testing machine and subjected to a compressive axial load applied parallel to the long axis of the tooth, running at a crosshead speed of 0.5 mm/min. For µTBS testing, 40 teeth were longitudinally sectioned to obtain resin-dentin bonded sticks from the cavity floor (bonded area: 0.8 mm2). Specimens were tested in tension at 0.5 mm/min. The external marginal integrity of both proximal surfaces was analyzed using SEM of epoxy resin replicas. The µTBS, marginal integrity, and fracture resistance data were subjected to two-way ANOVA, and Tukey's post-hoc test was used for pair-wise comparisons (a = 0.05). RESULTS: Fracture resistance, microtensile bond strength, and marginal integrity values were not statistically significantly affected by application technique or preparation size (p = 0.71, p = 0.82, and p = 0.77, respectively). CONCLUSIONS: The use of a bulk-fill flowable composite associated with a conventional resin composite as a final capping layer did not jeopardize the fracture strength, bond strength to dentin, or marginal integrity of posterior restorations.

Evaluation of the In Vitro Effects of Cervical Marginal Relocation Using Composite Resins on the Marginal Quality of CAD/CAM Crowns.

PURPOSE: To evaluate the effect of cervical margin relocation (CMR) for crowns designed using CAD/CAM technology, and made of pre-cured resin or lithium disilicate, before and after thermomechanical loading. The test hypothesis was that the marginal quality of the crowns would not be influenced by the CMR with composite resins before or after thermomechanical loading. MATERIALS AND METHODS: Standard crown preparations were created in 40 human molars. The margins were located in enamel, except for the mesial proximal box, where the cervical margin was 2.0 mm below the cementoenamel junction, with 4.0 mm in the buccolingual and 2.0 mm in the mesiodistal dimension. For the CMR technique, a 2-mm layer of conventional or flowable composite resin was applied to the mesial box. Using the Cerec CAD/CAM system, 40 standard crowns were prepared, and restorations were luted using a dual-curing adhesive cement. SEM analysis was performed using epoxy resin replicas before and after loading to assess the marginal quality of interfaces of the mesial proximal box with CMR/crown and the distal face of the tooth without CMR. Statistical differences between groups were analyzed using the Kruskal-Wallis test and Bonferroni's post-hoc test. RESULTS: The null hypothesis was accepted, since no statistically significant differences were found in marginal quality before and after thermomechanical cycling (p > 0.05). CONCLUSION: The implementation of CMR before and after thermomechanical cycling had no effect on the quality of cervical margins. To establish whether CMR is a suitable procedure for the adhesive luting of composite resin crowns in deep proximal boxes, additional studies are required.

Influence of Cavity Margin Design and Restorative Material on Marginal Quality and Seal of Extended Class II Resin Composite Restorations In Vitro.

PURPOSE: To investigate the influence of three cavity designs on the marginal seal of large Class II cavities restored with low-shrinkage resin composite limited to the enamel. MATERIALS AND METHODS: One hundred twenty (120) intact human molars were randomly divided into 12 groups, with three different cavity designs: 1. undermined enamel, 2. box-shaped, and 3. proximal bevel. The teeth were restored with 1. an extra-low shrinkage (ELS) composite free of diluent monomers, 2. microhybrid composite (Herculite XRV), 3. nanohybrid composite (Filtek Supreme XTE), and 4. silorane-based composite (Filtek Silorane). After artificial aging by thermocycling and storage in physiological saline, epoxy resin replicas were prepared. To determine the integrity of the restorations' approximal margins, two methods were sequentially employed: 1. replicas were made of the 120 specimens and examined using SEM, and 2. the same 120 specimens were immersed in AgNO3 solution, and the dye penetration depth was observed with a light microscope. Statistical analysis was performed using the Kruskal-Wallis and the Dunn-Bonferroni tests. RESULTS: After bevel preparation, SEM observations showed that restorations did not exhibit a higher percentage of continuous margin (SEM-analysis; p>0.05), but more leakage was found than with the other cavity designs (p<0.05). The lowest percentage of continuous margin was observed in ELS restorations (p<0.05). More fractured margins were observed in the undermined enamel cavity design groups (p<0.05). CONCLUSION: Bevel preparation failed to improve margin quality in large Class II composite restorations and is no longer recommended. However, undermined enamel should be removed to prevent enamel fractures.

Marginal fit of indirect composite inlays using a new system for manual fabrication.

AIM: This in vitro study compares a new system for manual chair side fabrication of indirect composite restorations, which uses silicone models after alginate impressions, to CAD/CAM-technology and laboratory manual production techniques. MATRIALS AND METHODS: and study design Each 10 composite inlays were fabricated using different types of production techniques: CAD/CAM- technology (A), the new inlay system (B), plaster model after alginate impression (C) or silicone impression (D). The inlays were adapted into a metal tooth and silicone replicas of the cement gaps were made and measured. Statistical analysis was performed using ANOVA and Tukey's test. Results and Statistics In group A the biggest marginal gaps (174.9µm ± 106.2µm) were found. In group B the gaps were significantly smaller (119.5 µm ± 90.6 µm) than in group A (p=0.035). Between groups C (64.6 µm ± 68.0µm) and D (58.2 µm ± 61.7 µm) no significant differences could be found (p=0.998), but the gaps were significantly smaller compared with group B. Conclusion Chairside manufacturing of composite inlays resulted in better marginal precision than CAD/CAM technology. In comparison to build restorations in a laboratory, the new system is a timesaving and inexpensive alternative. Nevertheless, production of indirect composite restorations in the dental laboratory showed the highest precision.

The Accuracy of Fit of Crowns Made From Wax Patterns Produced Conventionally (Hand Formed) and Via CAD/CAM Technology.

OBJECTIVE: The aim of this study was to compare the marginal and internal fit accuracy of crowns constructed using three different wax production methods; conventional, milled, and 3D printed and utilizing three different cement gap thicknesses. METHODS: 15 identical stone dies were made for each method. Wax patterns were produced and then cast into metal crowns, which were assessed for differences in accuracy of both marginal and internal fit. Measurement points were 4 points per crown; occlusal, axial, marginal gap, and marginal discrepancy points. A silicone impression technique and conventional cementation technique were employed to facilitate the measurements. RESULTS: There was no significant difference (p > 0.05) in accuracy between the digitally and manually produced crowns, with the 3D printed crowns being slightly more accurate. Whereas, statistically significant differences were noticed between the conventional versus printed groups, in occlusal and marginal gap points (p < 0.05). CONCLUSION: The marginal and internal fit of 3D printed wax patterns is more accurate than the other two production methods. The milling of wax crowns is as accurate as the conventional hand carved production in terms of internal and marginal fit. The manufacturer recommended offset/die-spacer of 30 µm produced the most accurate internal and marginal fits.

Microsphere skimming in the porcine coronary arteries: Implications for flow quantification.

Particle skimming is a phenomenon where particles suspended in fluid flowing through vessels distribute disproportionately to bulk fluid volume at junctions. Microspheres are considered a gold standard of intra-organ perfusion measurements and are used widely in studies of flow distribution and quantification. It has previously been hypothesised that skimming at arterial junctions is responsible for a systematic over-estimation of myocardial perfusion from microspheres at the subendocardium. Our objective is to integrate coronary arterial structure and microsphere distribution, imaged at high resolution, to test the hypothesis of microsphere skimming in a porcine left coronary arterial (LCA) network. A detailed network was reconstructed from cryomicrotome imaging data and a Poiseuille flow model was used to simulate flow. A statistical approach using Clopper-Pearson confidence intervals was applied to determine the prevalence of skimming at bifurcations in the LCA. Results reveal that microsphere skimming is most prevalent at bifurcations in the larger coronary arteries, namely the epicardial and transmural arteries. Bifurcations at which skimming was identified have significantly more asymmetric branching parameters. This finding suggests that when using thin transmural segments to quantify flow from microspheres, a skimming-related deposition bias may result in underestimation of perfusion in the subepicardium, and overestimation in the subendocardium.

Rate and pattern of interproximal dental attrition.

Although occlusal and interproximal attrition occur because of diverse etiology and present dissimilar features, both progress with age. The objectives of this study were to reveal the rate and pattern of development of interproximal attrition facets (PAF) with age and to compare those with occlusal attrition (OA) changes. Five-hundred and ninety-four teeth were collected from 198 skulls (of adults, 20-71 yr of age). Three mandibular teeth [first premolar (P1), second premolar (P2), and first molar (M1)] were examined for PAF size and OA rate. Interproximal attrition and OA followed similar patterns of development until subjects reached 40-45 yr of age, after which they took different paths: PAF did not increase in size and were not as large as in younger groups, regardless of facet location, whereas OA continued to progress. The PAF changes with age differed between premolars and molars, unlike OA, which presented a similar rate for all teeth studied. Although OA scores presented significantly moderate correlations with age, PAF area size demonstrated low correlations with age. Low, but significant, correlations were found between the rate of OA and that of PAF. However, PAF and OA exhibited different patterns of development with age. Premolars and molars presented dissimilar development of PAF, which is probably caused by a unique attrition pattern in the molar teeth, different morphology, and force vectors.

In vivo scanning electron microscope assessment of enamel permeability in primary teeth with and without early childhood caries.

BACKGROUND: Over the years, certain primary teeth have been shown to be highly sensitive to dental caries, while others have remained caries-free. It has been hypothesized that this may be attributed to differences in the permeability of the enamel surface. AIM: The aim of the study was to evaluate the hypothesized differences in the permeability of primary tooth enamel in children with and those without Severe Early Childhood Caries (S-ECC) using scanning electron microscopy. MATERIALS AND METHODS: Sixteen children between 3 and 6 years of age were randomly selected and divided into two groups: Group 1, children without S-ECC (n = 8), and Group 2, children with S-ECC (n = 8). In each child, 4 teeth (the maxillary right and left central and lateral incisors) were subjected to evaluation. An impression was made with polyvinylsiloxane impression material, and scanning electron microscopy was used to inspect the negative replicas for droplets. RESULTS: The results indicated higher significance when individual regions (cervical, middle, and incisal thirds) in the two groups were evaluated and compared. Similarly, the overall results showed high statistical significance between S-ECC and non-S-ECC teeth. CONCLUSION: There could be a positive relationship between the permeability of tooth enamel and the development of caries, which needs further research.

Comparison of marginal and internal fit of press-on-metal and conventional ceramic systems for three- and four-unit implant-supported partial fixed dental prostheses: An in vitro study.

STATEMENT OF PROBLEM: Adaptation is an important factor in the long-term clinical success of implant supported ceramic restorations. Ceramic firings may affect the adaptation of the restoration. PURPOSE: The purpose of this study was to compare the marginal and internal adaptation of 3 different restorative materials and the effect of veneering/pressing on the material used for 3- and 4-unit implant supported fixed dental prostheses. MATERIAL AND METHODS: One mandibular epoxy cast was prepared for 3-unit restorations and one for 4-unit restorations. Impressions of the casts were made and 60 stone die casts (30 3-unit, 30 4-unit) produced. The casts were divided into 3 subgroups: group MCR, conventional metal ceramic restorations; group POM, press-on-metal restorations; group ZIR, computer-aided design/computer-aided manufacturing CAD/CAM) zirconia restorations. A replica technique was used to examine the marginal and internal gap values. A total of 2400 measurements were made by making 40 measurements of each restoration. The data were evaluated statistically using analysis of variance and the least significant difference post hoc test (α=.05). RESULTS: The lowest marginal gaps were found in group POM (81.58 µm) and the highest in group MCR (103.82 µm). The differences in marginal adaptation measurements were found to be statistically significant. The highest values for internal adaptation were found at the occlusal surface in all groups. CONCLUSIONS: Although veneering metal ceramic restorations increased the misfit of the restoration, the marginal discrepancy of the materials (81 to 120 µm) can be considered clinically acceptable.

Clinical marginal and internal fit of metal ceramic crowns fabricated with a selective laser melting technology.

STATEMENT OF PROBLEM: Selective laser melting (SLM) technology has been introduced to fabricate dental restorations. However, the fit of these restorations still needs further study. PURPOSE: The purpose of this in vivo investigation was to compare the marginal and internal fit of SLM metal ceramic crowns with 2 lost-wax cast metal ceramic crowns and to evaluate the influence of tooth type on the marginal and internal fit of these crowns. MATERIAL AND METHODS: A total of 330 metal ceramic crowns were evaluated. The metal copings were fabricated with SLM Co-Cr, cast Au-Pt, and cast Co-Cr alloy (n=110). The marginal and internal gaps of crowns were recorded by using a replica technique. The anterior and premolar replicas were sectioned 2 times, and molar replicas were sectioned 4 times. The marginal and internal gap width of each cross section was examined by stereomicroscope at ×30 magnification. Two-way analysis of variance was performed to identify the statistical difference among the groups. RESULTS: The marginal fit of the SLM Co-Cr group (75.6 ±32.6 µm) was not different from the cast Au-Pt group (76.8 ±32.1 µm) (P>.05) but was better than the cast Co-Cr group (91.0 ±36.3 µm) (P<.01). No significant difference was found among the SLM Co-Cr group (127.3 ±45.8 µm), cast Au-Pt group (129.9 ±61.1 µm). and cast Co-Cr group (142.5 ±63.7 µm) (P>.05). The mean occlusal gap width of the SLM Co-Cr group (309.8 ±106.6 µm) was significantly higher than that of the cast Au-Pt group (254.6 ±109.6 µm) and the cast Co-Cr group (249.6 ±110.4 µm) (P<.005). No significant difference was found in the marginal fit among the anterior group (84.4 ±35.1 µm), the premolar group (80.6 ±26.3 µm), and the molar group (82.7 ±38.0 µm) (P>.05). Also, no significant difference was found in the axial fit among the anterior group (138.3 ±52.5 µm), the premolar group (132.9 ±50.4 µm), and the molar group (134.4 ±52.5 µm) (P>.05). The anterior group (267.6 ±110.2 µm) did not differ from the premolar group (270.2 ±112.8 µm) and the molar group (268.6 ±110.5 µm) in occlusal fit (P>.05). CONCLUSIONS: The marginal fit of SLM Co-Cr metal ceramic crowns was similar to that of the cast Au-Pt metal ceramic crowns and was better than that of the cast Co-Cr metal ceramic crowns. The SLM Co-Cr metal ceramic crowns were not significantly different from the 2 cast metal ceramic crowns in axial fit but were less accurate in occlusal fit. Tooth type did not influence the marginal and internal fit of the metal ceramic crowns.