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Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones / Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays
Chaves, Fernando; León, Guillermo; Hernández-Chavarría, Francisco.
Affiliation
  • Chaves, Fernando; Universidad de Costa Rica. Facultad de Microbiología. San José. CR
  • León, Guillermo; Universidad de Costa Rica. Facultad de Microbiología. San José. CR
  • Hernández-Chavarría, Francisco; Universidad de Costa Rica. Facultad de Microbiología. San José. CR
Rev. biol. trop ; 54(2): 253-256, jun. 2006.
Article in Es | LILACS | ID: lil-492073
Responsible library: BR1.1
ABSTRACT
Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice.
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Full text: 1 Database: LILACS Main subject: Tetanus Toxin / Immunoenzyme Techniques / Clostridium tetani / Antibodies, Bacterial / Antigens, Bacterial Type of study: Diagnostic_studies / Etiology_studies Limits: Animals Language: Es Year: 2006 Type: Article
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Full text: 1 Database: LILACS Main subject: Tetanus Toxin / Immunoenzyme Techniques / Clostridium tetani / Antibodies, Bacterial / Antigens, Bacterial Type of study: Diagnostic_studies / Etiology_studies Limits: Animals Language: Es Year: 2006 Type: Article