Subnanogram assay for pilocarpine in biological fluids.
J Pharm Sci
; 65(12): 1724-8, 1976 Dec.
Article
in En
| MEDLINE
| ID: mdl-1032652
ABSTRACT
A method for the determination of pilocarpine was developed in which the imidazole ring of pilocarpine was acylated with heptafluorobutyric anhydride, using triethylamine as a catalyst. After cleanup, the pilocarpine derivative was analyzed using GLC with electron-capture detection. The limit of sensitivity was 25-50 pg of pilocarpine, which had been subjected to the derivatization and cleanup procedures. The method was specific for pilocarpine, with the isopilocarpine derivative eluting prior to the pilocarpine derivative.
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Database:
MEDLINE
Main subject:
Pilocarpine
Limits:
Animals
Language:
En
Year:
1976
Type:
Article