Inhibition of DNA synthesis in human gliomas by roscovitine.
Neuroreport
; 10(12): 2563-7, 1999 Aug 20.
Article
in En
| MEDLINE
| ID: mdl-10574370
ABSTRACT
The early effect of 1-100 microM roscovitine, a purine analogue and cyclin-dependent kinase inhibitor, was studied on tissue specimens from eight human malignant gliomas. The tissue was incubated immediately after resection with DMEM containing [3H]methylthymidine plus vehicle alone or the proper concentration of roscovitine for 30-90 min. The DNA synthesis rate was assessed by measurement of [3H]methylthymidine incorporation into trichloroacetic acid insoluble material/mg protein/min. In all gliomas, 100 microM roscovitine inhibited DNA synthesis by 71-97% (average 89 +/- 8%, p<0.0001). This inhibitory effect of roscovitine appeared within 30 min of incubation and was concentration dependent.
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Database:
MEDLINE
Main subject:
Purines
/
DNA, Neoplasm
/
Glioblastoma
/
Antineoplastic Agents
Type of study:
Clinical_trials
Limits:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Year:
1999
Type:
Article