Molecular phylogeny of Anabaena circinalis and its identification in environmental samples by PCR.

Fergusson, K M; Saint, C P

Fergusson, K M; Saint, C P.

Affiliation

Fergusson KM; The Cooperative Research Centre for Water Quality and Treatment, Australian Water Quality Centre, SA Water Corporation, Salisbury, South Australia 5108, Australia.

Appl Environ Microbiol ; 66(9): 4145-8, 2000 Sep.

Article in En | MEDLINE | ID: mdl-10966445

ABSTRACT

ABSTRACT

Although the cyanobacterium Anabaena circinalis occurs worldwide, Australian isolates are believed to exclusively possess the saxitoxin group neurotoxins (paralytic shellfish poisons). Identification of A. circinalis in a mixed population is complicated due to limited morphological differences between Anabaena species. Sequence analysis of the DNA-dependent RNA polymerase (rpoC1) gene from 24 Anabaena isolates, including 12 designated A. circinalis, permitted a phylogenetic analysis to be performed. In addition, an A. circinalis-specific PCR was developed and tested successfully on environmental samples.

Subject(s)

Anabaena/classification; Anabaena/genetics; Phylogeny; Polymerase Chain Reaction/methods; Water Microbiology; DNA, Bacterial/genetics; DNA-Directed RNA Polymerases/genetics; Genes, Bacterial; Molecular Sequence Data; Sequence Analysis, DNA

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Database: MEDLINE Main subject: Phylogeny / Water Microbiology / Polymerase Chain Reaction / Anabaena Type of study: Diagnostic_studies Language: En Year: 2000 Type: Article

Fulltext

XML

PubMed Links

Search on Google