Mass spectral analysis of a protein complex using single-chain antibodies selected on a peptide target: applications to functional genomics.

Siegel, R W; Allen, B; Pavlik, P; Marks, J D; Bradbury, A

Siegel, R W; Allen, B; Pavlik, P; Marks, J D; Bradbury, A.

Affiliation

Siegel RW; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, 87545, USA.

J Mol Biol ; 302(2): 285-93, 2000 Sep 15.

Article in En | MEDLINE | ID: mdl-10970733

Subject(s)

Antibodies/immunology; Antigens, Nuclear; DNA Helicases; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/immunology; Epitopes/immunology; Genome; Nuclear Proteins/chemistry; Nuclear Proteins/immunology; Peptide Library; Antibodies/genetics; Antibody Affinity/immunology; Antibody Specificity/immunology; Biotinylation; Blotting, Western; DNA-Binding Proteins/metabolism; Dimerization; Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulin Variable Region/genetics; Immunoglobulin Variable Region/immunology; Ku Autoantigen; Macromolecular Substances; Nuclear Proteins/metabolism; Precipitin Tests; Protein Binding; Solubility; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thermodynamics

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PubMed Links

Database: MEDLINE Main subject: Nuclear Proteins / Genome / DNA Helicases / Peptide Library / Antigens, Nuclear / DNA-Binding Proteins / Antibodies / Epitopes Type of study: Prognostic_studies Limits: Humans Language: En Year: 2000 Type: Article

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