[Effect of cGMP on calcium-activated potassium channels in primary cultured porcine coronary artery smooth muscle cells].

ABSTRACT

Guanosine 3',5'-cyclic monophosphate (cGMP) is capable of relaxing vascular smooth muscle through activating calcium-activated potassium channels (KCa channel) in the vascular smooth muscle cell membrane. But regarding the mechanism it is still under debate. In the present work the mechanism of the effect of cGMP on KCa channel in primary cultured porcine coronary artery smooth muscle cells using patch clamp technique was investigated. Experimental results showed that (1) different concentrations of 8-bromo-cGMP (0.25 mmol/L, 0.50 mmol/L, 1.00 mmol/L), serving as a membrane permeable analogue, could activate KCa channels, and (2) 1.00 mmol/L 8-bromo-cGMP could not activate such kind of channels in inside-out patch under the same condition. These results suggested that the activating effect of cGMP on KCa channels was indirect, being mediated by some intracellular process.