Detex for detection of Escherichia coli O157 in raw ground beef and raw ground poultry.
J AOAC Int
; 84(3): 752-60, 2001.
Article
in En
| MEDLINE
| ID: mdl-11417639
ABSTRACT
A method for detection of Escherichia coli O157 in beef and poultry is presented. The method is antibody-based and uses a patented antibody-specific metal-plating procedure for the detection of E. coli O157 in enriched meat samples. Both raw ground beef and raw ground poultry were tested as matrixes for the organism. The sensitivity and specificity of the assay were 98 and 90%, respectively. The accuracy of the assay was 96%. Overall, the method agreement between the E. coli O157 Detex assay and the U.S. Department of Agriculture/Food Safety Inspection Service method was 96%. Sample temperature upon loading of the apparatus was critical to the observed false-positive rate of the system.
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Database:
MEDLINE
Main subject:
Poultry Products
/
Escherichia coli O157
/
Food Microbiology
/
Meat
Type of study:
Diagnostic_studies
Limits:
Animals
Country/Region as subject:
America do norte
Language:
En
Year:
2001
Type:
Article