Steps toward mapping the human vasculature by phage display.
Nat Med
; 8(2): 121-7, 2002 Feb.
Article
in En
| MEDLINE
| ID: mdl-11821895
ABSTRACT
The molecular diversity of receptors in human blood vessels remains largely unexplored. We developed a selection method in which peptides that home to specific vascular beds are identified after administration of a peptide library. Here we report the first in vivo screening of a peptide library in a patient. We surveyed 47,160 motifs that localized to different organs. This large-scale screening indicates that the tissue distribution of circulating peptides is nonrandom. High-throughput analysis of the motifs revealed similarities to ligands for differentially expressed cell-surface proteins, and a candidate ligand-receptor pair was validated. These data represent a step toward the construction of a molecular map of human vasculature and may have broad implications for the development of targeted therapies.
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Database:
MEDLINE
Main subject:
Blood Vessels
/
Peptide Library
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Year:
2002
Type:
Article