Decreased gene expression of calretinin and ryanodine receptor type 1 in tottering mice.
Brain Res Bull
; 59(1): 53-8, 2002 Oct 15.
Article
in En
| MEDLINE
| ID: mdl-12372549
ABSTRACT
Tottering mice are a spontaneously occurring animal model of human absence epilepsy. They carry a mutation in the P/Q-type calcium channel alpha1A subunit gene which is highly expressed by cerebellar Purkinje cells. In this study, we investigated the role of calretinin and ryanodine receptor type 1 (RyR1) gene expression in the cerebellum of tottering mice. Cerebellar tissue specimens from four experimental groups were processed for in situ hybridization histochemistry (ISHH) (1) wild-type (+/+); (2) heterozygous (tg/+) and two homozygous groups; either (3) without occurrence of an episode of paroxysmal dyskinesia (tg/tg-N); or (4) after an episode of paroxysmal dyskinesia (tg/tg-P) that lasted about 45 min on average. Quantitative analysis showed a statistically significant decrease (p = 0.0001, ANOVA) of calretinin gene expression at the level of the simple lobule of the cerebellum in both homozygous groups compared to the wild-type and heterozygous groups. RyR1 was decreased in the flocculus of the cerebellum in both the tg/tg-N and tg/tg-P groups compared to wild type (p = 0.0174, ANOVA). These results suggest that calretinin gene expression, as well as other genes involved in regulation of calcium homeostasis, such as RyR1, may play a role in the biochemical functional alterations present in tottering mice.
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Database:
MEDLINE
Main subject:
S100 Calcium Binding Protein G
/
Cerebellum
/
Gene Expression Regulation
/
Chorea
/
Epilepsy, Absence
/
Ryanodine Receptor Calcium Release Channel
/
Calcium Signaling
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Year:
2002
Type:
Article