Vaccination of mice with MUC1 cDNA suppresses the development of lung metastases.
Clin Exp Metastasis
; 19(8): 689-96, 2002.
Article
in En
| MEDLINE
| ID: mdl-12553374
ABSTRACT
C57BL/6 mice were immunized intradermally with various doses of purified pCEP4 plasmid DNA containing full-length MUC1 cDNA (22 tandem repeats). Mice immunized with MUC1 DNA three times at weekly intervals had serum antibodies to a synthetic peptide corresponding to the tandem repeats of MUC1. The antibody titer correlated with the plasmid DNA dose. After the third immunization mice were injected intravenously with 5 x 10(5) 16-F10 melanoma cells that had been stably transfected with MUC1 cDNA (F10-MUC1-C8 clone cells). The number of lung metastatic nodules three weeks after inoculation of F10-MUC1-C8 cells was significantly lower in mice immunized with MUC1 plasmid DNA than in mice immunized with the vector DNA alone. Thus, the suppression of lung metastasis was antigen-specific. In vivo depletion of lymphocyte subpopulations by specific antibodies revealed that natural killer cells are the major effector cells responsible for the suppression of lung metastasis. CD4+ cells and CD8+ cells apparently played some roles too.
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Database:
MEDLINE
Main subject:
Genetic Therapy
/
DNA, Complementary
/
Mucin-1
/
Cancer Vaccines
/
Lung Neoplasms
Limits:
Animals
/
Female
/
Humans
Language:
En
Year:
2002
Type:
Article