Effects of a point mutation in the 3' end of the S genome segment of naturally occurring and engineered Bunyamwera viruses.
J Gen Virol
; 84(Pt 4): 789-793, 2003 Apr.
Article
in En
| MEDLINE
| ID: mdl-12655079
ABSTRACT
The genome of Bunyamwera virus (BUN) consists of three segments of single-stranded RNA of negative polarity. The smallest segment, S, encodes the N protein and a nonstructural protein called NSs. We recently described a mutant virus (BUNdelNSs) that does not express NSs but overexpresses N and grows to lower titres than wild-type (wt) BUN. Here we report a BUNdelNSs variant that expresses lower levels of N protein and grows to higher titres. Sequencing of the 3' and 5' termini of the BUNdelNSs S RNA segment and analysis using a minireplicon system show that the N overexpressing phenotype results from a single nucleotide substitution at position 16 in the 3' terminus. This mutation could also be detected in wtBUN populations, and was isolated by plaquing a 'wt' variant carrying the mutation. This variant was found to express increased N and NSs levels, and grew to lower titres than wtBUN.
Full text:
1
Database:
MEDLINE
Main subject:
Bunyamwera virus
/
Point Mutation
/
3' Untranslated Regions
Language:
En
Year:
2003
Type:
Article